FastQCFastQC Report
Thu 26 May 2016
SRR937419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937419_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427780
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51771.210201505446725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42050.9829819065874983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31060.7260741502641544No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17300.4044134835663191No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10760.2515311608770864No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9920.23189489924727663No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9440.2206741783159568No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8210.19192108092944973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7620.17812894478470243No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6960.16270045350413764No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT4390.10262284351769602No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT250.005019810556.998047
ATACGTC856.401843E-427.9402145
GCGGTTA458.941362E-426.39106626-27
GTATAGA1309.986434E-625.5820221
GGTATCA47750.024.4760841
TAGGACA1751.4820944E-724.427734
GAGTACT37000.024.32686412-13
GTATCAA63700.024.3141081
GTTGTAG1601.8517949E-623.7547361
GTGTAAG1201.7060628E-423.7547341
GGGCGCG1000.001652285523.7491847
TACTAAT1000.001652285523.7491842
AAGGGTA1401.7765678E-523.7491824
GTACATG36050.023.7217881
AGTACTT37200.023.55765512-13
TACATGG37000.023.1073112
ACATGGG36200.022.9619163
GTACTTT40000.022.38360414-15
CGGTTAC550.002876371121.59142728-29
GCATAAG1100.002875020721.5901646
Produced by FastQC (version 0.11.5)