##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937419_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 427780 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21171630277245 33.0 31.0 34.0 31.0 34.0 2 32.39265744074057 34.0 31.0 34.0 31.0 34.0 3 32.42773154425172 34.0 31.0 34.0 31.0 34.0 4 36.089309925662725 37.0 35.0 37.0 35.0 37.0 5 36.02832063210061 37.0 35.0 37.0 35.0 37.0 6 36.09067277572584 37.0 35.0 37.0 35.0 37.0 7 36.03403384917481 37.0 35.0 37.0 35.0 37.0 8 36.01925989994857 37.0 35.0 37.0 35.0 37.0 9 37.694574313899665 39.0 37.0 39.0 35.0 39.0 10-11 37.62078171022488 39.0 37.0 39.0 35.0 39.0 12-13 37.50605918930292 39.0 37.0 39.0 35.0 39.0 14-15 38.63344242367572 40.0 38.0 41.0 34.0 41.0 16-17 38.62663284866053 40.0 38.0 41.0 34.5 41.0 18-19 38.697468324839875 40.0 38.0 41.0 35.0 41.0 20-21 38.648406891392774 40.0 38.0 41.0 34.0 41.0 22-23 38.573995979241666 40.0 38.0 41.0 34.0 41.0 24-25 38.555694515872645 40.0 38.0 41.0 34.0 41.0 26-27 38.36232876712329 40.0 38.0 41.0 34.0 41.0 28-29 38.18116672121184 40.0 37.5 41.0 34.0 41.0 30-31 38.09024966104072 40.0 37.0 41.0 34.0 41.0 32-33 37.8660806956847 40.0 36.5 41.0 33.5 41.0 34-35 37.67281780354388 40.0 36.0 41.0 33.0 41.0 36-37 37.44172939361354 40.0 36.0 41.0 33.0 41.0 38-39 37.25048389359016 40.0 35.0 41.0 33.0 41.0 40-41 36.986142409649815 39.5 35.0 41.0 32.5 41.0 42-43 36.666465239141615 39.0 35.0 41.0 31.5 41.0 44-45 36.14650053765955 39.0 35.0 41.0 30.5 41.0 46-47 35.51039202393754 39.0 35.0 41.0 28.0 41.0 48-49 34.9723794941325 39.0 35.0 40.5 24.5 41.0 50-51 34.6138482397494 39.0 35.0 40.0 23.0 41.0 52-53 34.31925171817289 38.0 34.5 40.0 22.0 41.0 54-55 34.07902192715882 38.0 34.0 40.0 20.5 41.0 56-57 33.76331642433027 38.0 34.0 40.0 18.5 41.0 58-59 33.48261372668194 38.0 33.5 40.0 16.0 41.0 60-61 33.109767871335734 37.0 33.0 40.0 11.0 41.0 62-63 32.71412174482211 36.5 33.0 40.0 7.5 41.0 64-65 32.25660736827341 36.0 32.0 39.0 3.5 41.0 66-67 31.81346954041797 35.5 31.5 39.0 2.0 40.0 68-69 31.348173126373368 35.0 31.0 38.5 2.0 40.0 70-71 30.877514142783674 35.0 31.0 38.0 2.0 40.0 72-73 30.398326242461078 35.0 30.0 37.0 2.0 39.0 74-75 29.919465613165645 34.5 30.0 36.5 2.0 39.0 76-77 28.625800645191454 33.5 28.0 35.0 2.0 37.5 78-79 29.2120494179251 34.0 29.0 36.0 2.0 37.5 80-81 29.018906914769275 34.0 29.0 35.0 2.0 37.0 82-83 28.635936696432744 34.0 29.0 35.0 2.0 37.0 84-85 28.2218687175651 34.0 29.0 35.0 2.0 36.0 86-87 27.878495955865162 34.0 28.0 35.0 2.0 36.0 88-89 27.46596965730048 34.0 27.0 35.0 2.0 35.5 90-91 26.92694024966104 33.0 25.5 35.0 2.0 35.0 92-93 26.442345364439664 33.0 25.0 35.0 2.0 35.0 94-95 25.98350203375567 33.0 24.0 35.0 2.0 35.0 96-97 25.34263289541353 32.0 20.5 34.5 2.0 35.0 98-99 24.45461218383281 32.0 9.0 34.0 2.0 35.0 100-101 21.823735331245032 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20950049520684644 1101 2 0.2254937704401243 1101 3 0.1748701551118188 1101 4 0.10961763576998607 1101 5 0.040627005085539736 1101 6 0.005819653263465341 1101 7 0.10799976369889919 1101 8 0.04588298799795609 1101 9 0.1130978264547835 1101 10-11 0.09665219358903698 1101 12-13 -0.015242257340155163 1101 14-15 -0.06476461182240456 1101 16-17 0.12209611280326271 1101 18-19 0.21872081187348869 1101 20-21 0.05745811052431549 1101 22-23 0.26355015211419897 1101 24-25 0.19473191586121175 1101 26-27 0.24304655112320717 1101 28-29 0.13632032893728763 1101 30-31 0.3956048277253785 1101 32-33 0.32209930135853426 1101 34-35 0.24176723316990234 1101 36-37 0.22808037792770364 1101 38-39 0.22589745333120703 1101 40-41 0.11229478926512826 1101 42-43 0.09810047183199799 1101 44-45 0.03476209054834811 1101 46-47 -0.054309821113015744 1101 48-49 -0.09155486044954131 1101 50-51 0.09155272097196132 1101 52-53 0.1781224140294526 1101 54-55 0.003965795624139901 1101 56-57 0.20913548868707466 1101 58-59 0.08839201751769821 1101 60-61 0.027716402169367882 1101 62-63 0.208304602044997 1101 64-65 0.3188156811854981 1101 66-67 0.32941694691176693 1101 68-69 0.3707118535561307 1101 70-71 0.40084890814046403 1101 72-73 0.24064814377878463 1101 74-75 0.47993086729957213 1101 76-77 0.3369855668017543 1101 78-79 0.578374718721193 1101 80-81 0.657168974249327 1101 82-83 0.6032196878536773 1101 84-85 0.5084153631825252 1101 86-87 0.5101246022277302 1101 88-89 0.7346608922780185 1101 90-91 1.0148287404595386 1101 92-93 1.188868093657021 1101 94-95 0.7740601153720981 1101 96-97 1.0812083800508923 1101 98-99 1.618844147798189 1101 100-101 1.8902461800670984 1102 1 0.23219424601548866 1102 2 0.29407745957627895 1102 3 0.31967543393184883 1102 4 0.08509096134034877 1102 5 0.1125703353999441 1102 6 0.17138225299607512 1102 7 0.14183984650392034 1102 8 0.05935389842190375 1102 9 0.1827575495754985 1102 10-11 0.09141757936554029 1102 12-13 0.23469089485718797 1102 14-15 0.14806154076310207 1102 16-17 0.13359393485873028 1102 18-19 0.2261473871096129 1102 20-21 0.2192276365518353 1102 22-23 0.2515628316328957 1102 24-25 0.18186371385146316 1102 26-27 0.21063830512328963 1102 28-29 -0.06631045555402437 1102 30-31 0.17318278088879424 1102 32-33 0.29840606808127035 1102 34-35 0.2665559078582973 1102 36-37 0.09243109168972552 1102 38-39 0.09249080305303181 1102 40-41 0.214083507081142 1102 42-43 0.5637202156539658 1102 44-45 0.6013381892243288 1102 46-47 0.5714794142343678 1102 48-49 0.3774406974061506 1102 50-51 0.2378329642117123 1102 52-53 0.2535429082719176 1102 54-55 0.3994492561839351 1102 56-57 0.47555093699862994 1102 58-59 0.4821244969242642 1102 60-61 0.5122799507094982 1102 62-63 0.449770725303722 1102 64-65 0.46602424632975925 1102 66-67 0.8615782873180287 1102 68-69 0.8635332621040241 1102 70-71 0.939181810197649 1102 72-73 0.8163090744727093 1102 74-75 0.6245043351910624 1102 76-77 0.6934536308031376 1102 78-79 0.6736037263547807 1102 80-81 0.7166311728951165 1102 82-83 0.5504228620461156 1102 84-85 0.7307015580330898 1102 86-87 0.7010462393519781 1102 88-89 0.6936982839198862 1102 90-91 0.8150282056771196 1102 92-93 0.9782888897927862 1102 94-95 0.8957576182832092 1102 96-97 1.2721785357415847 1102 98-99 1.5877653105186766 1102 100-101 1.8573247154533057 1103 1 0.05080012572704362 1103 2 0.1450243942549534 1103 3 0.19788933287265564 1103 4 0.06024614240693893 1103 5 0.0021395263452888003 1103 6 0.02389288455000127 1103 7 0.14895044107852584 1103 8 0.1756749845572969 1103 9 0.1870524332227177 1103 10-11 0.07766259376106177 1103 12-13 0.007977299675069105 1103 14-15 0.19826492701206888 1103 16-17 0.02798628542266357 1103 18-19 0.15517615173625643 1103 20-21 0.2897221028610417 1103 22-23 0.2388155324437946 1103 24-25 0.16017138177718948 1103 26-27 0.11263386760513328 1103 28-29 0.3268272395779164 1103 30-31 0.06580012418893944 1103 32-33 -0.003959287580535431 1103 34-35 0.26286325881844164 1103 36-37 0.1257745503125065 1103 38-39 0.2618191001554422 1103 40-41 0.024330740235747328 1103 42-43 0.18813184829085827 1103 44-45 0.32706654971985216 1103 46-47 0.31371111607541025 1103 48-49 0.32242867912780326 1103 50-51 0.06451618352547683 1103 52-53 0.24334586133459624 1103 54-55 0.2593002823600088 1103 56-57 0.3025537632750783 1103 58-59 0.27445392982000527 1103 60-61 0.46264446112974156 1103 62-63 0.6682458567123675 1103 64-65 0.7178758536093994 1103 66-67 0.9805737705584754 1103 68-69 0.9599343820490809 1103 70-71 0.881443887907043 1103 72-73 0.5766646042014365 1103 74-75 0.6986888091602665 1103 76-77 0.5648289972945904 1103 78-79 0.7751740266796716 1103 80-81 1.0991029761651454 1103 82-83 0.87916635764822 1103 84-85 1.1464864440575475 1103 86-87 1.0477128179726485 1103 88-89 1.1181574613941976 1103 90-91 1.1098207252893637 1103 92-93 1.0879893573170207 1103 94-95 1.1807664669057338 1103 96-97 1.1939461471417765 1103 98-99 1.5477021815400995 1103 100-101 2.099808669070679 1104 1 0.1328786366445982 1104 2 0.25383002604113614 1104 3 0.08392918491895074 1104 4 0.12369322509397307 1104 5 0.07461297820067614 1104 6 0.08222721219586049 1104 7 0.27088696416646485 1104 8 0.204484195868595 1104 9 0.27221530161551044 1104 10-11 0.10561868491963367 1104 12-13 0.2219046353152052 1104 14-15 0.28360378872308445 1104 16-17 0.20524436918354638 1104 18-19 0.18865067744918207 1104 20-21 0.32112945281927097 1104 22-23 0.29746306853733984 1104 24-25 0.13042254512285467 1104 26-27 0.31644904479162506 1104 28-29 0.29347501430647327 1104 30-31 0.3613704618574758 1104 32-33 0.3943523697396003 1104 34-35 0.42913816634737856 1104 36-37 0.43904862261891253 1104 38-39 0.41837261405593296 1104 40-41 0.48012062211142137 1104 42-43 0.5143190574544434 1104 44-45 0.7926911768526494 1104 46-47 0.862595497414155 1104 48-49 0.9595215081458193 1104 50-51 0.8047321482074992 1104 52-53 0.8927045297512635 1104 54-55 0.975804876083906 1104 56-57 1.1958391707443496 1104 58-59 1.3663045443051587 1104 60-61 1.275855891746609 1104 62-63 1.307101601850178 1104 64-65 0.9987104795490254 1104 66-67 1.242231090687337 1104 68-69 1.248351635360148 1104 70-71 1.2994608309925972 1104 72-73 1.353364878631698 1104 74-75 1.4852834875995917 1104 76-77 1.4010806379102725 1104 78-79 1.314581615272914 1104 80-81 1.2888804490204144 1104 82-83 1.1831485793401377 1104 84-85 1.2528192205758621 1104 86-87 1.4097788005522922 1104 88-89 1.2666451120325455 1104 90-91 0.7851190190490591 1104 92-93 0.8950827829225574 1104 94-95 1.209945562479053 1104 96-97 1.2676247057863321 1104 98-99 1.3688392773520555 1104 100-101 2.2279288217970823 1105 1 -0.0736075518774797 1105 2 0.034359210827076936 1105 3 -0.1285287069166614 1105 4 -0.10226684105412431 1105 5 -0.12265535033487396 1105 6 -0.24032438944558976 1105 7 -0.22036372487622913 1105 8 -0.2200977848994583 1105 9 -0.018912890948953986 1105 10-11 -0.21515978019948534 1105 12-13 -0.030360018326000215 1105 14-15 -0.23818986595934177 1105 16-17 -0.21548203493233586 1105 18-19 -0.04040854959804818 1105 20-21 0.0310685108213562 1105 22-23 -0.13306152263066195 1105 24-25 -0.09931806858450187 1105 26-27 -0.185050005860667 1105 28-29 -0.09087759180609822 1105 30-31 -0.3293703355946036 1105 32-33 -0.2853193396987521 1105 34-35 -0.16394996986870325 1105 36-37 -0.38398071528762756 1105 38-39 -0.480201833178711 1105 40-41 -0.4697829119119845 1105 42-43 -0.23933593121463304 1105 44-45 -0.23459797608420274 1105 46-47 -0.6710083489752563 1105 48-49 -0.25946085236132177 1105 50-51 -0.19558940504784772 1105 52-53 -0.3991234239450705 1105 54-55 -0.35445634765591194 1105 56-57 -0.06252886992448481 1105 58-59 -0.3650668037685918 1105 60-61 -0.042630358023707515 1105 62-63 -0.1634992433755258 1105 64-65 -0.20775580329534193 1105 66-67 -0.15434513274623995 1105 68-69 -0.34488809024774625 1105 70-71 -0.12898897607545123 1105 72-73 -0.09188991404301206 1105 74-75 0.14551500594343736 1105 76-77 0.11377280426048841 1105 78-79 0.06483120184730495 1105 80-81 0.02044932266529642 1105 82-83 -0.20355595915437874 1105 84-85 -0.012123818561761368 1105 86-87 0.3292633966201599 1105 88-89 0.5565743703163726 1105 90-91 0.505828579899184 1105 92-93 0.8346920191570142 1105 94-95 0.9859546272184438 1105 96-97 0.8848844595684078 1105 98-99 1.04912959400545 1105 100-101 1.6716156595504934 1106 1 -0.12262218437346206 1106 2 0.15368406727449013 1106 3 -0.06483928798745353 1106 4 0.14243980418537916 1106 5 0.0035675485345549873 1106 6 -0.08296162195848922 1106 7 0.03170615774427432 1106 8 -0.009336274848422477 1106 9 0.005977388825868957 1106 10-11 -0.15898631416811781 1106 12-13 -0.25997367156309537 1106 14-15 0.015317891988914312 1106 16-17 0.03203692528644808 1106 18-19 -0.04858705289199605 1106 20-21 -0.11405117115445051 1106 22-23 -0.18329318032235165 1106 24-25 -0.05958384057318966 1106 26-27 -0.07858379593087506 1106 28-29 -0.08473607855979282 1106 30-31 -0.15398713712082213 1106 32-33 -0.13775855892305344 1106 34-35 -0.5306410710180671 1106 36-37 0.025520069435188475 1106 38-39 0.14778786674704492 1106 40-41 0.18355521433910837 1106 42-43 0.5046447279525239 1106 44-45 0.6611184711420108 1106 46-47 0.5007637148927202 1106 48-49 0.7565977716013919 1106 50-51 0.723177687342691 1106 52-53 0.4938676386729881 1106 54-55 0.3300239966823355 1106 56-57 0.2666975907406979 1106 58-59 0.30002101940093695 1106 60-61 0.41432971039284183 1106 62-63 0.4787399890058026 1106 64-65 0.2617692279526054 1106 66-67 0.41675216348427213 1106 68-69 0.018927260246112354 1106 70-71 0.39074891710826876 1106 72-73 0.44011455934689536 1106 74-75 0.3759603713799251 1106 76-77 -0.0743116262751009 1106 78-79 0.2602530255994999 1106 80-81 -0.013578043955316588 1106 82-83 0.3064974207924287 1106 84-85 0.5345789359562723 1106 86-87 0.10240332162509347 1106 88-89 0.21793593487509355 1106 90-91 0.14473861401348032 1106 92-93 0.38714627498605125 1106 94-95 0.42709571756023834 1106 96-97 0.5382413815754212 1106 98-99 0.3017985913458503 1106 100-101 0.8075630126381697 1107 1 0.16565620390492342 1107 2 0.1074488210392417 1107 3 0.06483104168287923 1107 4 -0.029110867365290005 1107 5 0.07878122374823704 1107 6 -0.04596528496215058 1107 7 -0.0224248963867808 1107 8 0.16001659450444805 1107 9 0.0018666847151678212 1107 10-11 0.10635760117579451 1107 12-13 0.005146393556962892 1107 14-15 -0.05786078118975979 1107 16-17 0.0910723343218578 1107 18-19 0.08256882826388079 1107 20-21 0.05719425009096568 1107 22-23 0.20099660396742536 1107 24-25 0.19015160916226392 1107 26-27 -0.06997573732281381 1107 28-29 -0.02279061661433701 1107 30-31 -0.07759316072684186 1107 32-33 -0.06775448891898606 1107 34-35 -0.2954355358125369 1107 36-37 -0.17474448082936078 1107 38-39 -0.07557395661477528 1107 40-41 -0.12297714219324263 1107 42-43 0.028026901334698096 1107 44-45 -0.03684652682298406 1107 46-47 0.05556631969532333 1107 48-49 0.08978865479227949 1107 50-51 -0.13329361912861515 1107 52-53 -0.2536293088239603 1107 54-55 -0.04282907617073306 1107 56-57 -0.31116158711847675 1107 58-59 -0.3141425947626786 1107 60-61 0.09286043892357299 1107 62-63 -0.3135880533222348 1107 64-65 -0.017005700822323888 1107 66-67 0.0837240804561894 1107 68-69 0.2844543257731793 1107 70-71 0.09426947062882007 1107 72-73 0.26315078238311784 1107 74-75 0.3600466554662063 1107 76-77 0.38714543518196365 1107 78-79 0.40007801542449073 1107 80-81 0.49995471957744186 1107 82-83 0.2620326263276347 1107 84-85 0.20991471129204697 1107 86-87 0.4138807331025056 1107 88-89 0.7977080146471742 1107 90-91 0.8968753661502369 1107 92-93 0.7324454202851953 1107 94-95 0.7388580293225537 1107 96-97 0.8684204617544999 1107 98-99 0.9241632637105219 1107 100-101 1.6639936190687763 1108 1 -0.04096295818298756 1108 2 0.07365761224803435 1108 3 0.08304017629826177 1108 4 -0.04400612835430451 1108 5 -0.03524693559242564 1108 6 0.14787915185103628 1108 7 0.0808546365802556 1108 8 0.1475337648341153 1108 9 -0.02196573286725112 1108 10-11 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0.47730868044858 2316 66-67 0.267718347059148 2316 68-69 0.3596334944306072 2316 70-71 -0.07842522597891843 2316 72-73 0.1784398411504391 2316 74-75 0.13288468699989053 2316 76-77 0.29519608018913246 2316 78-79 0.329461675242932 2316 80-81 0.38457010419282867 2316 82-83 0.16009760482739566 2316 84-85 0.32864380828201334 2316 86-87 0.5642390704173614 2316 88-89 0.4687065812979121 2316 90-91 0.40067374168773995 2316 92-93 0.6885610437921237 2316 94-95 0.5144433136034898 2316 96-97 0.641162936670888 2316 98-99 0.7491990974420091 2316 100-101 1.150305133641158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 6.0 5 17.0 6 38.0 7 55.0 8 150.0 9 266.0 10 485.0 11 926.0 12 1496.0 13 2186.0 14 2668.0 15 4027.0 16 5727.0 17 8769.0 18 7357.0 19 3866.0 20 3132.0 21 3125.0 22 3144.0 23 3439.0 24 3775.0 25 4171.0 26 4800.0 27 5751.0 28 6660.0 29 8083.0 30 9902.0 31 12236.0 32 15469.0 33 20327.0 34 28362.0 35 42235.0 36 61249.0 37 86149.0 38 62880.0 39 4848.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.048892464643295 19.875061685661564 15.32447242680163 19.751573422893507 2 15.708775538828371 22.63406423862733 39.06704380756464 22.590116414979665 3 19.22039366029267 27.55411660199168 31.16508485670204 22.060404881013604 4 12.470194960026182 18.193697695076906 37.01575576230773 32.32035158258918 5 12.662613171754575 39.40305625100811 32.748451887540064 15.18587868969725 6 29.188601617653937 37.90079012576558 17.193650942073027 15.716957314507457 7 26.641264201224928 36.30978540371218 20.037168638084996 17.011781756977886 8 27.31006592173547 33.6614614989013 19.333769694702884 19.69470288466034 9 28.251437654869328 16.455187245780543 20.141427836738508 35.15194726261162 10-11 24.81836457992426 28.126373369488988 26.007059703585956 21.048202347000792 12-13 27.046550363811694 26.324420677361854 27.23695976154993 19.392069197276527 14-15 23.260788255645426 26.466524849221564 27.985529945298985 22.287156949834028 16-17 20.20407686193838 30.360699424938055 28.074711300201038 21.36051241292253 18-19 20.17134975922203 27.368624059095797 33.11889288886811 19.341133292814064 20-21 21.51791172300618 26.018839188972798 33.22490494496568 19.238344143055343 22-23 21.031075807109552 26.64674193182655 32.87522806886306 19.44695419220083 24-25 20.952733826656022 26.88803460661392 33.05160719482817 19.10762437190188 26-27 20.740351123267175 26.98120485307525 33.1116020291278 19.166841994529772 28-29 20.036024705326795 27.36603531870526 33.37065938535915 19.227280590608796 30-31 20.0743377106089 27.387251550163345 33.04404743119963 19.494363308028124 32-33 19.89943393602063 27.66396628161537 32.788697889211356 19.647901893152646 34-35 20.414932909439433 27.312052924400394 32.77794660806957 19.495067558090607 36-37 20.74304635916401 27.192280134976084 32.405246160697274 19.659427345162637 38-39 20.408995755991114 27.386422210507984 32.65747891144854 19.547103122052363 40-41 20.241596147552478 27.800855579971014 32.535766047968586 19.421782224507925 42-43 20.16330013559639 28.198438303642398 31.61546733997288 20.022794220788327 44-45 20.312193183412035 28.40712515779139 31.146383655149844 20.134298003646734 46-47 20.706321006124643 28.79786338772266 30.209336574874936 20.28647903127776 48-49 21.073215204076863 28.97552480246856 29.47753518163542 20.47372481181916 50-51 20.972930010753192 29.32278273879097 29.232783206321006 20.471504044134836 52-53 21.19605872177287 29.873533124503247 28.599864416288746 20.330543737435132 54-55 20.112674739352006 29.529314133433072 29.08317359390341 21.274837533311516 56-57 20.82694375613633 30.127168170554956 28.456683341904714 20.589204731403992 58-59 20.922553649071954 29.088199541820565 28.79505820748983 21.194188601617654 60-61 20.610009818130813 28.9796156903081 29.788092009911637 20.622282481649446 62-63 20.811164617326664 29.17480948150919 29.613820187947077 20.400205713217073 64-65 20.575529477768946 29.24388704474262 29.53305437374351 20.647529103744915 66-67 20.229206601524147 29.41102903361541 29.153653747253262 21.206110617607184 68-69 20.52059469820936 29.483729954649583 29.009654495301323 20.986020851839733 70-71 20.419257562298377 29.734092290429658 28.81866847444948 21.02798167282248 72-73 20.35838557013602 29.397271257739092 28.63098862848734 21.613354543637552 74-75 20.368179905558932 29.80749450652204 28.275982981906587 21.548342606012437 76-77 20.315933423722473 29.235822151573238 28.98008322034691 21.46816120435738 78-79 20.133596708588527 29.2411987470195 28.84543456917107 21.77976997522091 80-81 19.846767029781663 29.516340174856236 28.857940997709104 21.778951797652997 82-83 19.817194686490055 29.6432140540351 28.576070503941885 21.963520755532958 84-85 19.695287297208846 29.502080508672684 28.296437421104304 22.506194773014165 86-87 19.868507176586096 28.987329935948384 28.183061386694096 22.961101500771424 88-89 19.853312450324935 28.756019449249614 28.254593482631257 23.136074617794193 90-91 19.559624104712018 28.78540091717702 28.62303042488699 23.031944553223973 92-93 19.40854144604732 28.87991995754758 28.471058248183034 23.24048034822207 94-95 19.499041563420448 28.909486184487353 28.11024358315022 23.48122866894198 96-97 19.85868904577119 29.005096077422976 27.778180373088972 23.358034503716866 98-99 19.695145612911368 29.323153598361767 26.91115485134813 24.070545937378736 100-101 19.33946438445361 29.084889768501576 27.23458576827713 24.34106007876768 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 878.0 1 601.5 2 371.0 3 427.0 4 393.5 5 481.5 6 795.0 7 1419.0 8 1995.0 9 2135.5 10 1966.5 11 1729.5 12 1716.0 13 1849.5 14 1885.0 15 1792.5 16 1818.5 17 1833.0 18 1840.0 19 1843.0 20 1853.0 21 2042.0 22 2266.5 23 2481.0 24 2738.5 25 3161.5 26 3590.5 27 4073.5 28 4683.0 29 5242.0 30 5841.0 31 6453.0 32 7105.5 33 7746.0 34 8815.0 35 9658.0 36 10311.5 37 11213.0 38 11571.5 39 11499.0 40 11367.0 41 12036.0 42 13036.5 43 13774.0 44 14547.5 45 14924.5 46 14740.0 47 15549.5 48 19172.5 49 18763.5 50 15026.0 51 13490.5 52 12212.0 53 11546.0 54 10954.5 55 10082.0 56 9013.5 57 8032.0 58 7505.5 59 6833.0 60 5948.5 61 5100.0 62 4151.5 63 2986.0 64 1911.0 65 1383.0 66 1007.5 67 780.0 68 580.0 69 371.0 70 289.5 71 229.5 72 199.5 73 159.0 74 118.5 75 101.5 76 77.0 77 47.0 78 28.5 79 16.5 80 5.5 81 3.5 82 2.0 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0488568890551218 2 0.0 3 0.0 4 0.0 5 2.337650194024966E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004090887839543691 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.506475291037449E-4 22-23 8.181775679087382E-4 24-25 1.168825097012483E-4 26-27 0.002337650194024966 28-29 0.004207770349244939 30-31 5.844125485062415E-4 32-33 3.506475291037449E-4 34-35 0.0 36-37 1.168825097012483E-4 38-39 1.168825097012483E-4 40-41 0.0 42-43 0.009350600776099864 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.168825097012483E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.844125485062415E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012740193557436065 92-93 0.0016363551358174763 94-95 0.0 96-97 0.0 98-99 4.675300388049932E-4 100-101 0.015545373790266026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 427780.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.06447801838353 #Duplication Level Percentage of deduplicated Percentage of total 1 78.83623776588944 43.41076281511877 2 11.894885541914059 13.099713269078295 3 3.918456796091302 6.473033343430651 4 1.708600316794286 3.7633273834528835 5 0.9584830746733032 2.6389185098170382 6 0.5411656819673533 1.7879403479396916 7 0.37147861849646435 1.4318693355748628 8 0.2798908422028706 1.2329634502421458 9 0.19643726067292344 0.9735043700084103 >10 1.1396799569840625 11.782247663375282 >50 0.0971569832063035 3.7022236655879976 >100 0.05326588991453129 5.125812902356216 >500 0.0021306355965812515 0.989027300683518 >1k 0.001704508477265001 2.3739001663143893 >5k 4.2612711931625023E-4 1.2147554770198274 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5177 1.210201505446725 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4205 0.9829819065874983 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3106 0.7260741502641544 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1730 0.4044134835663191 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1076 0.2515311608770864 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 992 0.23189489924727663 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 944 0.2206741783159568 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 821 0.19192108092944973 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 762 0.17812894478470243 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 696 0.16270045350413764 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 439 0.10262284351769602 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.337650194024966E-4 0.0 24-25 0.0 0.0 0.0 0.0014025901164149797 0.0 26-27 0.0 0.0 0.0 0.0021038851746224695 0.0 28-29 0.0 0.0 0.0 0.005376595446257421 0.0 30-31 0.0 0.0 0.0 0.010519425873112347 0.0 32-33 0.0 0.0 0.0 0.01601290382907102 0.0 34-35 0.0 0.0 0.0 0.02349338444995091 0.0 36-37 0.0 0.0 0.0 0.028636214876805836 0.0 38-39 0.0 0.0 0.0 0.03716863808499696 0.0 40-41 0.0 0.0 0.0 0.04955818411332928 0.0 42-43 0.0 0.0 0.0 0.07246715601477395 0.0 44-45 0.0 0.0 0.0 0.10683061386694095 0.0 46-47 0.0 0.0 0.0 0.14458366450044413 0.0 48-49 0.0 0.0 0.0 0.24895974566365886 0.0 50-51 0.0 0.0 0.0 0.3429332834634625 0.0 52-53 0.0 0.0 0.0 0.4433353592968348 0.0 54-55 0.0 0.0 0.0 0.6200617139651223 0.0 56-57 0.0 0.0 0.0 0.7962036560849035 0.0 58-59 0.0 0.0 0.0 1.0302024405068027 0.0 60-61 0.0 0.0 0.0 1.2811491888353828 0.0 62-63 0.0 0.0 0.0 1.6218617046145214 0.0 64-65 0.0 0.0 0.0 1.8750292206274253 0.0 66-67 0.0 0.0 0.0 2.2328065828229464 0.0 68-69 0.0 0.0 0.0 2.6919210809294496 0.0 70-71 0.0 0.0 0.0 2.9968675487400067 0.0 72-73 0.0 0.0 0.0 3.5143064191874327 0.0 74-75 0.0 0.0 0.0 4.176212071625602 0.0 76-77 0.0 0.0 0.0 4.694936649679741 0.0 78-79 0.0 0.0 0.0 5.184323717798868 0.0 80-81 0.0 0.0 0.0 5.849852728037776 0.0 82-83 0.0 0.0 0.0 6.64044602365702 0.0 84-85 0.0 0.0 0.0 7.412104352704661 0.0 86-87 0.0 0.0 0.0 8.475384543456917 0.0 88-89 0.0 0.0 0.0 9.771027163495255 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGGT 25 0.0050198105 56.99804 7 ATACGTC 85 6.401843E-4 27.940214 5 GCGGTTA 45 8.941362E-4 26.391066 26-27 GTATAGA 130 9.986434E-6 25.582022 1 GGTATCA 4775 0.0 24.476084 1 TAGGACA 175 1.4820944E-7 24.42773 4 GAGTACT 3700 0.0 24.326864 12-13 GTATCAA 6370 0.0 24.314108 1 GTTGTAG 160 1.8517949E-6 23.754736 1 GTGTAAG 120 1.7060628E-4 23.754734 1 GGGCGCG 100 0.0016522855 23.749184 7 TACTAAT 100 0.0016522855 23.749184 2 AAGGGTA 140 1.7765678E-5 23.749182 4 GTACATG 3605 0.0 23.721788 1 AGTACTT 3720 0.0 23.557655 12-13 TACATGG 3700 0.0 23.107311 2 ACATGGG 3620 0.0 22.961916 3 GTACTTT 4000 0.0 22.383604 14-15 CGGTTAC 55 0.0028763711 21.591427 28-29 GCATAAG 110 0.0028750207 21.590164 6 >>END_MODULE