FastQCFastQC Report
Thu 26 May 2016
SRR937418_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937418_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427852
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74491.741022596598824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53921.2602488711049615No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39950.9337340949674187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12180.2846778792666623No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11740.27439394930957434No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10340.24167235399156717No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8200.19165505829118482No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA7460.1743593579088096No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT7010.16384170227087871No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.14701345325018933No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA6220.14537737348428897No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5800.1355608948888868No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5510.1287828501444425No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.12434206220842722No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.12247225676168395No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4900.11452558361302508No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG4370.10213812252835093No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35400.034.51581
GTATCAA55150.033.0172841
TATGACG600.003935190431.688014
AAGTGCG600.00394876531.665737
GTACATG29600.027.1445751
TATCAAC68450.026.662022
ATCAACG68900.026.4189053
TGCTAGG908.9092925E-426.4035782
TCAACGC69300.026.2694954
CAACGCA69800.026.1494165
GTATAGC1109.404673E-525.9326341
AACGCAG71100.025.7200556
TACATGG31000.025.449642
TAGACTG950.001220486825.0168485
GGGAACG950.001225502524.999267
AGTACTT44450.024.84459712-13
ACATGGG31200.024.8295173
CATGGGG8250.024.7742614
GAGTACT44300.024.66067112-13
ACGCAGA76700.023.6564067
Produced by FastQC (version 0.11.5)