##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937418_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 427852 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.907189869394088 33.0 31.0 34.0 30.0 34.0 2 31.966350513729047 33.0 31.0 34.0 30.0 34.0 3 31.966974561296897 33.0 31.0 34.0 30.0 34.0 4 35.634565223488494 37.0 35.0 37.0 33.0 37.0 5 35.65325392892869 37.0 35.0 37.0 35.0 37.0 6 35.72869122967755 37.0 35.0 37.0 35.0 37.0 7 35.67354832979628 37.0 35.0 37.0 33.0 37.0 8 35.678232192440376 37.0 35.0 37.0 33.0 37.0 9 37.305874461262306 39.0 37.0 39.0 34.0 39.0 10-11 37.200524947879174 39.0 37.0 39.0 34.0 39.0 12-13 37.067439207950414 39.0 37.0 39.0 33.0 39.0 14-15 38.142612398679915 40.0 38.0 41.0 33.0 41.0 16-17 38.15252587343287 40.0 38.0 41.0 33.5 41.0 18-19 38.24478441143199 40.0 38.0 41.0 33.5 41.0 20-21 38.25641109542552 40.0 38.0 41.0 34.0 41.0 22-23 38.12388045398876 40.0 38.0 41.0 33.5 41.0 24-25 37.95668946271141 40.0 38.0 41.0 33.0 41.0 26-27 37.775578938511444 40.0 37.0 41.0 33.0 41.0 28-29 37.7502757963034 40.0 37.0 41.0 33.0 41.0 30-31 37.44275941213317 39.0 36.5 41.0 33.0 41.0 32-33 37.079276245056704 39.0 35.0 41.0 32.5 41.0 34-35 36.8661826986902 39.0 35.0 41.0 32.0 41.0 36-37 36.76098160111441 39.0 35.0 41.0 31.0 41.0 38-39 36.593776586296194 39.0 35.0 41.0 31.0 41.0 40-41 36.30819418864468 39.0 35.0 41.0 30.5 41.0 42-43 35.91854075708422 39.0 35.0 41.0 30.0 41.0 44-45 35.45782186363509 39.0 35.0 41.0 28.0 41.0 46-47 34.77543052270411 39.0 35.0 41.0 23.5 41.0 48-49 34.16717112459449 39.0 34.5 40.5 17.0 41.0 50-51 33.16967432663631 37.5 33.5 39.5 10.0 40.5 52-53 33.1876104353842 38.0 33.5 39.5 7.5 40.5 54-55 33.45074348139076 38.0 34.0 40.0 4.5 41.0 56-57 33.2382716453353 38.0 34.0 40.0 2.0 41.0 58-59 32.993940661724146 38.0 33.0 40.0 2.0 41.0 60-61 32.687700419771325 37.5 33.0 40.0 2.0 41.0 62-63 32.34755359329861 37.0 33.0 40.0 2.0 41.0 64-65 31.955967250357602 36.5 32.0 40.0 2.0 41.0 66-67 31.568909342482915 36.0 32.0 39.0 2.0 41.0 68-69 31.104296579190937 35.0 31.0 39.0 2.0 40.5 70-71 30.6407496050036 35.0 31.0 38.5 2.0 40.0 72-73 30.248082280788687 35.0 30.5 37.5 2.0 40.0 74-75 29.809056636406982 35.0 30.0 37.0 2.0 39.0 76-77 29.388607509138673 35.0 29.5 36.5 2.0 39.0 78-79 28.9278500509522 34.5 29.0 36.0 2.0 39.0 80-81 28.457685134111795 34.0 28.5 36.0 2.0 37.5 82-83 27.999959098005853 34.0 27.5 35.0 2.0 37.0 84-85 27.588892888194984 34.0 26.5 35.0 2.0 36.5 86-87 27.180200162673074 34.0 26.0 35.0 2.0 36.0 88-89 26.789026345558746 34.0 25.0 35.0 2.0 36.0 90-91 26.389700176696614 33.5 24.5 35.0 2.0 35.0 92-93 25.97827636659406 33.0 23.5 35.0 2.0 35.0 94-95 25.492786056860783 33.0 19.5 35.0 2.0 35.0 96-97 24.92514467619644 32.5 12.5 35.0 2.0 35.0 98-99 24.23956882286398 32.0 2.0 35.0 2.0 35.0 100-101 22.565112468797622 30.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08042779939310307 1101 2 -0.012632972979520929 1101 3 0.08693990991110212 1101 4 0.06501006878514204 1101 5 0.16094647584028365 1101 6 0.01202081473782357 1101 7 0.17408283724954288 1101 8 0.20260102853218598 1101 9 0.35002317145832507 1101 10-11 0.17352379946836294 1101 12-13 0.19110544571957888 1101 14-15 0.18440102503202382 1101 16-17 0.2514279641743613 1101 18-19 0.2178187850787836 1101 20-21 0.11539407783936895 1101 22-23 0.1784341446401143 1101 24-25 -0.03558478085319905 1101 26-27 0.11630390238816801 1101 28-29 -0.013900634873429851 1101 30-31 0.05725321586769638 1101 32-33 0.07800207555872873 1101 34-35 0.2352170560281337 1101 36-37 0.6827603229859633 1101 38-39 0.5379802819297694 1101 40-41 0.701755336434978 1101 42-43 0.6784530717425739 1101 44-45 0.705975070099079 1101 46-47 0.7036769517192241 1101 48-49 0.704236527451215 1101 50-51 0.5599365868611983 1101 52-53 0.842740357160487 1101 54-55 1.0706325101594487 1101 56-57 1.0913515859885834 1101 58-59 1.0350941531339188 1101 60-61 1.1229904890118476 1101 62-63 1.2523475682250371 1101 64-65 1.2664166606408251 1101 66-67 1.1152058315023332 1101 68-69 1.2080849529953 1101 70-71 1.341087261805864 1101 72-73 1.32841791029648 1101 74-75 1.4104021751314058 1101 76-77 1.375233272456665 1101 78-79 1.2142794053466766 1101 80-81 1.3503173926890213 1101 82-83 1.3419876250714005 1101 84-85 1.365505620881585 1101 86-87 1.425431320178678 1101 88-89 1.399995735988309 1101 90-91 1.2036300789897325 1101 92-93 1.1410652180441971 1101 94-95 1.3048805963465888 1101 96-97 1.5612213902515713 1101 98-99 1.7174545967446342 1101 100-101 2.229237089467997 1102 1 -0.05998074307226631 1102 2 -0.1649367573481264 1102 3 0.10627727300507672 1102 4 0.018959861417108925 1102 5 0.20120880228225246 1102 6 -0.044946594376966686 1102 7 -0.038977036840037727 1102 8 -0.07515257590839042 1102 9 0.11711045419228583 1102 10-11 0.10762858892513094 1102 12-13 0.10585817208001203 1102 14-15 0.18633456534139015 1102 16-17 0.3446837990953 1102 18-19 0.12946883094279116 1102 20-21 -0.1430594035131918 1102 22-23 0.06501284649271355 1102 24-25 0.01822800775684641 1102 26-27 -0.03140172124472684 1102 28-29 0.21528122179567077 1102 30-31 0.2593655082056685 1102 32-33 0.1732825908036162 1102 34-35 0.36655079704153337 1102 36-37 0.5245824176775002 1102 38-39 0.8738155556634126 1102 40-41 0.8154539746267631 1102 42-43 0.9081014084863099 1102 44-45 1.1097419947017855 1102 46-47 1.2563588201483213 1102 48-49 1.3179929856522534 1102 50-51 0.9998537572479407 1102 52-53 1.2322325994792465 1102 54-55 1.056447987889932 1102 56-57 0.9800078881735885 1102 58-59 1.2599218691063498 1102 60-61 1.2492213692087901 1102 62-63 1.21327006197264 1102 64-65 1.180862646952189 1102 66-67 1.1789538617020234 1102 68-69 1.042501186501898 1102 70-71 1.155486992109818 1102 72-73 1.4425889085638381 1102 74-75 1.4130373955530366 1102 76-77 1.4985280721838343 1102 78-79 1.2104721047375087 1102 80-81 1.3270796289709814 1102 82-83 1.4974156499398852 1102 84-85 1.461724976276681 1102 86-87 1.4052276369460905 1102 88-89 1.414068538239956 1102 90-91 1.2638991086327778 1102 92-93 1.4458227268054031 1102 94-95 1.4456497788696616 1102 96-97 1.5138746426760896 1102 98-99 1.2839338873813233 1102 100-101 1.612739570828392 1103 1 0.19822261694994836 1103 2 0.17137260367544727 1103 3 0.1583226959951034 1103 4 0.07779390930562613 1103 5 0.0886021975203306 1103 6 0.1397831018073532 1103 7 0.14140011366253447 1103 8 0.10144002665727925 1103 9 0.13439651889354565 1103 10-11 0.3480546713815684 1103 12-13 0.25380475504765343 1103 14-15 0.21131279209451037 1103 16-17 0.15381446802835086 1103 18-19 0.200998680276129 1103 20-21 -0.00346238739467708 1103 22-23 -0.034604491322895115 1103 24-25 0.04109622807265367 1103 26-27 0.4113377949894854 1103 28-29 0.27482947425594517 1103 30-31 0.1730309028378798 1103 32-33 0.286235253450819 1103 34-35 0.5500256389488243 1103 36-37 0.6255755722528207 1103 38-39 0.5756367247289873 1103 40-41 0.5826685136860732 1103 42-43 0.42314283024777666 1103 44-45 0.6240218787460989 1103 46-47 0.8477265166439878 1103 48-49 1.032762399604799 1103 50-51 0.9301131008108499 1103 52-53 1.1579395463020319 1103 54-55 1.3543848938589562 1103 56-57 1.520537682810854 1103 58-59 1.423981706616928 1103 60-61 1.5206296249508213 1103 62-63 1.3518720301900125 1103 64-65 1.4755051660011453 1103 66-67 1.6508832027825626 1103 68-69 1.878671703632289 1103 70-71 1.7618803326518169 1103 72-73 1.964314822303912 1103 74-75 1.983269264854652 1103 76-77 2.022984582489837 1103 78-79 2.0664920393546566 1103 80-81 2.058744872137126 1103 82-83 1.9509807824516585 1103 84-85 1.9107818896507176 1103 86-87 2.062251665354392 1103 88-89 1.9817065511734775 1103 90-91 2.2964401201736493 1103 92-93 2.1448691217026763 1103 94-95 2.1000547065656505 1103 96-97 1.9892677181524725 1103 98-99 1.9529721199406325 1103 100-101 1.8962903561378646 1104 1 0.18437712875226708 1104 2 0.23103319691615454 1104 3 0.2387034469205389 1104 4 0.12002745576974405 1104 5 0.20590425030625426 1104 6 0.18353099854308397 1104 7 0.1765666921924165 1104 8 0.23874111795096553 1104 9 0.35859247101122804 1104 10-11 0.39731912982107787 1104 12-13 0.44290624055316385 1104 14-15 0.5305883076947211 1104 16-17 0.46684028409487865 1104 18-19 0.3621928536331751 1104 20-21 0.4472370982069833 1104 22-23 0.6834018545258616 1104 24-25 0.4056099533743236 1104 26-27 0.5663784180364573 1104 28-29 0.6662409448583233 1104 30-31 0.6895805879491732 1104 32-33 0.5548401282164477 1104 34-35 0.49614601777676626 1104 36-37 0.4409570442974413 1104 38-39 0.7478039282288265 1104 40-41 0.729139837180135 1104 42-43 1.0406612187867879 1104 44-45 1.0085706985143759 1104 46-47 0.9318811245955274 1104 48-49 0.9924878935714361 1104 50-51 0.9869733479143505 1104 52-53 0.9522162637675393 1104 54-55 1.1489981336070443 1104 56-57 1.063780796122714 1104 58-59 0.9292819325676049 1104 60-61 1.0686561621365342 1104 62-63 1.0227772751301565 1104 64-65 1.2425716531892697 1104 66-67 1.3329778136185801 1104 68-69 1.6210879336212294 1104 70-71 1.743720148045309 1104 72-73 1.6373473688161013 1104 74-75 1.4610107195924051 1104 76-77 1.3258666554670988 1104 78-79 1.6202033993854243 1104 80-81 1.6752056192165945 1104 82-83 1.6417889166759743 1104 84-85 1.6099790436336967 1104 86-87 1.8278158209238349 1104 88-89 1.520959471706142 1104 90-91 1.589683233485509 1104 92-93 1.8476598529175234 1104 94-95 2.0320291308721714 1104 96-97 1.577676929248092 1104 98-99 1.515517189889195 1104 100-101 1.2651908897660569 1105 1 -0.06833086352543205 1105 2 0.04866848266911816 1105 3 -0.06594030755482905 1105 4 0.1661563109108144 1105 5 0.11267998730270534 1105 6 0.06183897462722854 1105 7 0.06615702059261963 1105 8 0.16039974266283252 1105 9 0.08431162925008095 1105 10-11 -0.1290637110848607 1105 12-13 -0.036286308388064015 1105 14-15 -0.00797259145233653 1105 16-17 -0.24096096027849256 1105 18-19 -0.24312849984426066 1105 20-21 -0.14360747151259545 1105 22-23 0.1447055873929557 1105 24-25 0.04028591783959712 1105 26-27 0.34250001138089914 1105 28-29 -0.030040104382734967 1105 30-31 -0.15445018992334525 1105 32-33 0.0750529722345803 1105 34-35 0.33621949602553514 1105 36-37 0.28250565405557637 1105 38-39 0.424957607581284 1105 40-41 0.05268703506282435 1105 42-43 -0.16089791678108867 1105 44-45 -0.09090326402284887 1105 46-47 0.2953264549060677 1105 48-49 0.3854016957924813 1105 50-51 0.4106270690158169 1105 52-53 0.616768069222239 1105 54-55 0.5958187571094484 1105 56-57 0.8720579791528849 1105 58-59 0.9487154618260618 1105 60-61 0.8446765457719394 1105 62-63 0.6760732958174209 1105 64-65 0.7150097191729365 1105 66-67 0.6793984057459816 1105 68-69 0.8926399086021881 1105 70-71 0.9258331031410911 1105 72-73 0.8690454906169265 1105 74-75 0.6640842054319052 1105 76-77 0.4820446226194868 1105 78-79 0.5923062082078729 1105 80-81 0.6603037546807293 1105 82-83 0.6524007546695927 1105 84-85 0.8122327308299084 1105 86-87 0.6876568204709237 1105 88-89 0.35877091295770214 1105 90-91 0.7693140204669078 1105 92-93 0.7877538261323735 1105 94-95 0.5067532711589529 1105 96-97 0.8370837391433632 1105 98-99 0.6417358180421928 1105 100-101 0.7437205151130151 1106 1 -0.23934038708230787 1106 2 -0.20762528855329165 1106 3 -0.24117441727469213 1106 4 -0.1948302659143124 1106 5 -0.35837131787556586 1106 6 -0.23409428089059503 1106 7 -0.2514529380236823 1106 8 -0.3445941558940433 1106 9 -0.28445428755807 1106 10-11 -0.7283663559278111 1106 12-13 -0.7982537585153935 1106 14-15 -0.9752536027002137 1106 16-17 -0.6230223858939397 1106 18-19 -0.34384873541301886 1106 20-21 -0.46754436136828303 1106 22-23 -0.29126573582436066 1106 24-25 -0.2388965551018316 1106 26-27 -0.31096620519380025 1106 28-29 -0.16092932339801536 1106 30-31 -0.22179818440552168 1106 32-33 0.11484996661064173 1106 34-35 0.2140795474898809 1106 36-37 0.04701813548596334 1106 38-39 0.059854214648346726 1106 40-41 0.1291855362300609 1106 42-43 0.35768164073164854 1106 44-45 0.6174494126537198 1106 46-47 1.1034111986454533 1106 48-49 1.5836123812763532 1106 50-51 1.2476820615879731 1106 52-53 0.9576812297697757 1106 54-55 0.8153281214982471 1106 56-57 1.1565891628328444 1106 58-59 1.0645351111257213 1106 60-61 1.0302864248041956 1106 62-63 1.049521407022855 1106 64-65 0.9696056975260738 1106 66-67 1.0299727372400405 1106 68-69 0.9887426118067744 1106 70-71 0.6911182129670657 1106 72-73 1.0563465305150572 1106 74-75 1.1355185088062676 1106 76-77 1.3599444661042064 1106 78-79 1.286192827477823 1106 80-81 1.3699446980168908 1106 82-83 1.0786412709047362 1106 84-85 0.9324295023706561 1106 86-87 1.0177564994819583 1106 88-89 1.0958899043626253 1106 90-91 1.2932870547411 1106 92-93 1.2623830971425605 1106 94-95 1.1469869829586123 1106 96-97 1.3615757276832632 1106 98-99 1.5485893303511915 1106 100-101 1.4038336570909458 1107 1 -0.12356420559461156 1107 2 0.10213946256527962 1107 3 -0.02962956967961361 1107 4 -0.06363444656307848 1107 5 0.02306918827655835 1107 6 0.18419780523915819 1107 7 -0.07412928729661417 1107 8 0.10740353705897832 1107 9 0.26360919118913273 1107 10-11 0.20645738218740206 1107 12-13 0.22664749288281172 1107 14-15 0.21933794511857485 1107 16-17 0.20811095093491616 1107 18-19 -0.011345589439869741 1107 20-21 -0.08022700258752025 1107 22-23 -0.13703877133890074 1107 24-25 0.08967193972479492 1107 26-27 0.2977686766574337 1107 28-29 0.24182515087491652 1107 30-31 0.3249806886369839 1107 32-33 0.2960907908556081 1107 34-35 0.38295058006818294 1107 36-37 0.6430511507264569 1107 38-39 0.7460018831326849 1107 40-41 0.8218042967166426 1107 42-43 1.0139272713244907 1107 44-45 0.8915794636883945 1107 46-47 0.9384158302170462 1107 48-49 1.1629870317237803 1107 50-51 1.3852703236125805 1107 52-53 1.2528193904241363 1107 54-55 1.3317265065653672 1107 56-57 1.3126447242142802 1107 58-59 1.1398178117688929 1107 60-61 1.3398414443791395 1107 62-63 1.6967354730183821 1107 64-65 1.6274024676602998 1107 66-67 1.6646291270610725 1107 68-69 1.1707000188257872 1107 70-71 1.3881588134983858 1107 72-73 1.3529943213427984 1107 74-75 1.4245263912167836 1107 76-77 1.259502719040448 1107 78-79 1.5708597038760352 1107 80-81 1.6278140003099573 1107 82-83 1.560108430440529 1107 84-85 1.6685413417162955 1107 86-87 1.753116800796274 1107 88-89 1.7276078678693345 1107 90-91 1.8814595065028428 1107 92-93 1.9148622987644828 1107 94-95 1.9830905204306468 1107 96-97 1.7908350257816998 1107 98-99 2.043966497577145 1107 100-101 2.1564247155361365 1108 1 -0.0813472755231679 1108 2 0.13129440230692424 1108 3 -0.027796561091896876 1108 4 0.032143860429776794 1108 5 -0.04238509475728591 1108 6 0.1560098857760721 1108 7 0.1706278751483623 1108 8 -0.13863817471124662 1108 9 0.02794490599031718 1108 10-11 0.005288297970039935 1108 12-13 0.10717718906524709 1108 14-15 0.3796901136976345 1108 16-17 0.16158572902541124 1108 18-19 0.00627727265150213 1108 20-21 0.19165002707258338 1108 22-23 0.021162859605745155 1108 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2316 62-63 -0.9053637214356449 2316 64-65 -0.6594240702604885 2316 66-67 -0.32052442185086605 2316 68-69 -0.7229475747050849 2316 70-71 -1.0117713312523016 2316 72-73 -0.5471922540699232 2316 74-75 -1.0660740986542088 2316 76-77 -0.9113198928384811 2316 78-79 -0.8600985509806769 2316 80-81 -1.5374555613704608 2316 82-83 -1.071714614717795 2316 84-85 -1.3785844242597776 2316 86-87 -1.7142369231959087 2316 88-89 -1.6044212060622733 2316 90-91 -1.3917850487668062 2316 92-93 -1.8360374191617872 2316 94-95 -2.080424701823997 2316 96-97 -1.8661447515657485 2316 98-99 -2.3049648939571057 2316 100-101 -2.954468282138304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1385.0 3 271.0 4 226.0 5 225.0 6 276.0 7 301.0 8 431.0 9 503.0 10 769.0 11 1176.0 12 1929.0 13 2975.0 14 3327.0 15 4727.0 16 6705.0 17 9612.0 18 7885.0 19 4048.0 20 3705.0 21 3585.0 22 3723.0 23 3997.0 24 4246.0 25 4504.0 26 5060.0 27 5666.0 28 6445.0 29 7720.0 30 9278.0 31 11292.0 32 14291.0 33 18488.0 34 25892.0 35 38674.0 36 54852.0 37 78422.0 38 70923.0 39 10318.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.863972742057186 18.55072667538396 15.525685549447202 20.05961503311165 2 15.107510886956032 21.189214007453963 41.20879764434148 22.494477461248525 3 18.256373977493137 27.417941774500342 32.00130243715038 22.324381810856142 4 11.550922401171302 17.301786237188875 38.464187408491945 32.68310395314788 5 11.990891925954731 38.070015976611366 34.12013849519999 15.818953602233915 6 28.495467061269597 37.50842901830034 18.207381946915916 15.788721973514154 7 25.560352295021328 36.181410397479056 21.03281812605644 17.225419181443176 8 26.318870031629938 32.337454668049546 20.723578701416674 20.62009659890384 9 27.494855425156096 15.887196271090415 21.12073304631405 35.49721525743944 10-11 23.941420899129252 27.343860680277203 27.11777288890086 21.596945531692686 12-13 26.732494754982767 25.607625880413607 28.290194627603775 19.36968473699985 14-15 23.124893177151502 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19.572675885205662 40-41 19.34458351427498 26.101012844028105 35.135492615825086 19.418911025871825 42-43 19.27506069253874 26.527380119067406 34.20764924980042 19.98990993859343 44-45 19.405675650049574 26.772572490784647 33.705888343530994 20.115863515634786 46-47 19.69552773436814 26.98179136450633 32.80153114080525 20.521149760320277 48-49 20.01095701828628 27.201500268657657 32.15369793514054 20.63384477791552 50-51 19.91725920532313 27.712313019617575 31.88810091848369 20.4823268565756 52-53 20.170862319671997 27.859666704911852 31.359686608018844 20.609784367397303 54-55 19.59271923591544 27.58264257226506 31.618692866616488 21.20594532520301 56-57 20.084393124476932 28.030105286567913 31.000836908238572 20.88466468071658 58-59 20.29403846378949 27.193992812913347 31.389425384228208 21.122543339068955 60-61 20.166886911819613 26.88204262650831 32.22118309687704 20.729887364795033 62-63 20.49196639064669 26.933837145873863 32.10938725438554 20.46480920909391 64-65 20.072711609276933 27.0975988313704 32.06216772363034 20.767521835722327 66-67 19.951075633792925 27.272173974109883 31.87949156113205 20.897258830965143 68-69 19.97134727500647 27.133358068937426 31.981497456017248 20.913797200038857 70-71 19.821412266551793 27.401557843180868 31.652966668149435 21.124063222117908 72-73 19.97260963461955 27.14654430742759 31.48124520814922 21.399600849803644 74-75 19.75486678738773 27.487413834072132 31.419939577039273 21.337779801500858 76-77 19.797828931812912 27.147833496815032 31.568043112348214 21.48629445902384 78-79 19.61435189628655 26.984130699920645 31.70828485616033 21.69323254763248 80-81 19.49633559210534 27.14869150028269 31.696045529726714 21.65892737788526 82-83 19.50207420309398 27.271673783571188 31.432019515301345 21.794232498033487 84-85 19.455193256222802 26.795090582636316 31.002577373887764 22.747138787253117 86-87 19.857980879970786 26.59446717665896 31.030136049963016 22.517415893407243 88-89 19.705834504697357 26.25544669640234 31.18687947454229 22.85183932435801 90-91 19.339751280175566 26.269729334308707 31.884125822970006 22.50639356254572 92-93 19.522943382445888 26.43791170976305 31.718213782415926 22.320931125375132 94-95 19.12097069629224 26.492017177026604 31.509942099515797 22.87707002716536 96-97 19.297112186306936 26.636025926420086 31.207207713320646 22.85965417395233 98-99 19.606053051397566 26.69084797262105 30.52392439420913 23.17917458177226 100-101 19.846017535461264 26.277793448605603 31.063703280613026 22.81248573532011 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1040.0 1 699.0 2 469.5 3 579.0 4 446.5 5 418.5 6 826.5 7 1853.5 8 3067.5 9 3451.0 10 2678.0 11 1892.5 12 1784.0 13 1853.5 14 1894.0 15 1892.5 16 1848.0 17 1853.0 18 1900.5 19 1925.0 20 2035.5 21 2259.5 22 2507.0 23 2750.0 24 2975.5 25 3374.5 26 3911.0 27 4399.5 28 4869.5 29 5434.0 30 6042.5 31 6646.5 32 7468.0 33 8146.5 34 8980.0 35 9639.0 36 10216.0 37 11099.0 38 11467.0 39 11387.0 40 11488.5 41 12180.5 42 13038.0 43 13702.5 44 14268.5 45 14642.5 46 14737.5 47 15581.0 48 19069.5 49 18648.5 50 14569.0 51 12880.0 52 11708.5 53 11151.5 54 10849.0 55 9989.5 56 8773.5 57 7579.5 58 6837.5 59 6090.5 60 5202.0 61 4430.5 62 3547.5 63 2633.0 64 1854.0 65 1370.0 66 958.0 67 681.5 68 504.0 69 344.5 70 258.0 71 194.0 72 149.0 73 121.5 74 101.0 75 81.0 76 54.0 77 35.0 78 28.0 79 16.0 80 9.5 81 6.5 82 2.5 83 2.5 84 2.0 85 1.0 86 1.5 87 1.0 88 0.0 89 2.5 90 2.5 91 0.5 92 1.5 93 1.5 94 0.5 95 1.0 96 2.5 97 3.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26037040845899984 2 0.2255452820134065 3 0.22437665360919196 4 0.22834999018352142 5 0.22858371586436432 6 0.17669661471723866 7 0.16594523339846487 8 0.1694511186111085 9 0.16430915363256454 10-11 0.18826603591896263 12-13 0.18230603105746848 14-15 0.17365818086628088 16-17 0.17377504370670233 18-19 0.16851621588773688 20-21 0.16547778203677907 22-23 0.16804876452605105 24-25 0.16033581705823508 26-27 0.15682993184559146 28-29 0.16384170227087871 30-31 0.17377504370670233 32-33 0.16734758748352233 34-35 0.17517739779175978 36-37 0.16150444546244963 38-39 0.16489346783467182 40-41 0.16092013126034235 42-43 0.16337425090919291 44-45 0.16629582191972925 46-47 0.1681656273664725 48-49 0.1702691584940587 50-51 0.1717883754195376 52-53 0.1830072080999972 54-55 0.1698017071323729 56-57 0.15998522853697072 58-59 0.1629067995475071 60-61 0.15811542309022747 62-63 0.165711507717622 64-65 0.16010209137739217 66-67 0.1547264007180053 68-69 0.15601189196264129 70-71 0.16898366724942268 72-73 0.1623224853453998 74-75 0.16278993670708564 76-77 0.1610369941007638 78-79 0.15776483456896312 80-81 0.1641922907921431 82-83 0.16360797659003581 84-85 0.15729738320727726 86-87 0.15332404663294785 88-89 0.1504024756224115 90-91 0.15531071492011256 92-93 0.15659620616474856 94-95 0.15273973243084057 96-97 0.15028561278199004 98-99 0.15484326355842676 100-101 0.15437581219674093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 427852.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.38597416595373 #Duplication Level Percentage of deduplicated Percentage of total 1 80.11379025945462 45.17294107906244 2 11.461504633134242 12.925362082937328 3 3.568901638648377 6.03707986792972 4 1.576479359888861 3.5556529783941033 5 0.8590335329191519 2.4218721297433636 6 0.4770572188000378 1.6139601608964402 7 0.3619890397671925 1.428777325126992 8 0.2098455756607192 0.9465877766436003 9 0.17184811130608216 0.8720840848115421 >10 1.068654746178692 11.445209331659765 >50 0.08036013146630186 3.1097003983221376 >100 0.04432234054921433 4.405759246787985 >500 0.003728047335915224 1.332495175895958 >1k 0.001656909927073433 1.733294776568607 >5k 8.284549635367165E-4 2.9992235852199816 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7449 1.741022596598824 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5392 1.2602488711049615 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3995 0.9337340949674187 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1218 0.2846778792666623 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1174 0.27439394930957434 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1034 0.24167235399156717 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 820 0.19165505829118482 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 746 0.1743593579088096 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 701 0.16384170227087871 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 629 0.14701345325018933 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 622 0.14537737348428897 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 580 0.1355608948888868 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 551 0.1287828501444425 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 532 0.12434206220842722 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 524 0.12247225676168395 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 490 0.11452558361302508 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 437 0.10213812252835093 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 2.3372568084290829E-4 0.0 0.0 0.0 0.0 16-17 2.3372568084290829E-4 0.0 0.0 0.0 0.0 18-19 2.3372568084290829E-4 0.0 0.0 0.0 0.0 20-21 2.3372568084290829E-4 0.0 0.0 1.1686284042145414E-4 0.0 22-23 2.3372568084290829E-4 0.0 0.0 9.349027233716333E-4 0.0 24-25 2.3372568084290829E-4 0.0 0.0 0.0025709824892719913 0.0 26-27 2.3372568084290829E-4 0.0 0.0 0.0029215710105363535 0.0 28-29 2.3372568084290829E-4 0.0 0.0 0.004090199414750895 0.0 30-31 2.3372568084290829E-4 0.0 0.0 0.007362358946551612 0.0 32-33 2.3372568084290829E-4 0.0 0.0 0.01378981516973159 0.0 34-35 2.3372568084290829E-4 0.0 0.0 0.019516094350382845 0.0 36-37 2.3372568084290829E-4 0.0 0.0 0.02454119648850537 0.0 38-39 2.3372568084290829E-4 0.0 0.0 0.030384338509578078 0.0 40-41 2.3372568084290829E-4 0.0 0.0 0.03844787449865841 0.0 42-43 2.3372568084290829E-4 0.0 0.0 0.05550984920019072 0.0 44-45 2.3372568084290829E-4 0.0 0.0 0.08519301066724008 0.0 46-47 2.3372568084290829E-4 0.0 0.0 0.11884950870861886 0.0 48-49 2.3372568084290829E-4 0.0 0.0 0.19539466918467135 0.0 50-51 2.3372568084290829E-4 0.0 0.0 0.26691472752260126 0.0 52-53 2.3372568084290829E-4 0.0 0.0 0.3529257780727916 0.0 54-55 2.3372568084290829E-4 0.0 0.0 0.5168843431840917 0.0 56-57 2.3372568084290829E-4 0.0 0.0 0.6682217215298748 0.0 58-59 2.3372568084290829E-4 0.0 0.0 0.8790422856501781 0.0 60-61 2.3372568084290829E-4 0.0 0.0 1.1041201163018988 0.0 62-63 2.3372568084290829E-4 0.0 0.0 1.4101838953656873 0.0 64-65 2.3372568084290829E-4 0.0 0.0 1.650220169591354 0.0 66-67 2.3372568084290829E-4 0.0 0.0 1.9817600478670196 0.0 68-69 2.3372568084290829E-4 0.0 0.0 2.403167450426783 0.0 70-71 2.3372568084290829E-4 0.0 0.0 2.688429643895553 0.0 72-73 2.3372568084290829E-4 0.0 0.0 3.1827594588783037 0.0 74-75 2.3372568084290829E-4 0.0 0.0 3.807625066611819 0.0 76-77 2.3372568084290829E-4 0.0 0.0 4.2664285781064475 0.0 78-79 2.3372568084290829E-4 1.1686284042145414E-4 0.0 4.693562259846862 0.0 80-81 2.3372568084290829E-4 2.3372568084290829E-4 0.0 5.346007497919841 0.0 82-83 2.3372568084290829E-4 2.3372568084290829E-4 0.0 6.117886558903546 0.0 84-85 2.3372568084290829E-4 2.3372568084290829E-4 0.0 6.867327954526331 0.0 86-87 2.3372568084290829E-4 2.3372568084290829E-4 0.0 7.940596280956966 0.0 88-89 2.3372568084290829E-4 2.3372568084290829E-4 0.0 9.25249852752821 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3540 0.0 34.5158 1 GTATCAA 5515 0.0 33.017284 1 TATGACG 60 0.0039351904 31.68801 4 AAGTGCG 60 0.003948765 31.66573 7 GTACATG 2960 0.0 27.144575 1 TATCAAC 6845 0.0 26.66202 2 ATCAACG 6890 0.0 26.418905 3 TGCTAGG 90 8.9092925E-4 26.403578 2 TCAACGC 6930 0.0 26.269495 4 CAACGCA 6980 0.0 26.149416 5 GTATAGC 110 9.404673E-5 25.932634 1 AACGCAG 7110 0.0 25.720055 6 TACATGG 3100 0.0 25.44964 2 TAGACTG 95 0.0012204868 25.016848 5 GGGAACG 95 0.0012255025 24.99926 7 AGTACTT 4445 0.0 24.844597 12-13 ACATGGG 3120 0.0 24.829517 3 CATGGGG 825 0.0 24.774261 4 GAGTACT 4430 0.0 24.660671 12-13 ACGCAGA 7670 0.0 23.656406 7 >>END_MODULE