FastQCFastQC Report
Thu 26 May 2016
SRR937418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937418_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427852
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53071.2403821882333144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44211.0333012350064976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32680.7638155249946244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18800.4394042799846676No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10780.25195628394865516No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10760.2514888325869693No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9560.22344175088582033No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8580.20053663416321532No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8220.19212250965287062No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6930.16197189682413546No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT4660.10891616727279527No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4460.1042416536559371No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGAC902.4086186E-531.6682623
GCTTATA2300.030.990691
ATAGGAG2101.8189894E-1229.4062445
CCGCCGA350.00838354927.14739890-91
AGCGTCG350.00839311427.14105252-53
GTGCTAT908.917417E-426.3994771
TTATAGG2355.456968E-1226.2779223
GTATGTA1457.8406447E-726.2174111
GGTATCA49750.025.5982071
GTACATG34100.025.5014291
CTTATAG3200.025.2356472
TATAGGA2900.024.5702044
TACATGG35400.024.2879492
GAGTACT39350.023.05437912-13
ACATGGG35650.022.9184073
GTATCAA65800.022.8929211
AGTACTT39650.021.9216212-13
CATGGGG9450.021.6148474
TCCTACG1100.002867761821.5995711
ACGTCTA1553.8975344E-521.4526945
Produced by FastQC (version 0.11.5)