##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937418_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 427852 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18109299477389 33.0 31.0 34.0 31.0 34.0 2 32.37661387582622 34.0 31.0 34.0 31.0 34.0 3 32.42911333825715 34.0 31.0 34.0 31.0 34.0 4 36.075794901040545 37.0 35.0 37.0 35.0 37.0 5 35.9499219356226 37.0 35.0 37.0 35.0 37.0 6 36.05969353888728 37.0 35.0 37.0 35.0 37.0 7 36.0222389985322 37.0 35.0 37.0 35.0 37.0 8 36.015643259818816 37.0 35.0 37.0 35.0 37.0 9 37.69643942297804 39.0 37.0 39.0 35.0 39.0 10-11 37.62515075306415 39.0 37.0 39.0 35.0 39.0 12-13 37.53620644521938 39.0 37.0 39.0 35.0 39.0 14-15 38.64364546618924 40.0 38.0 41.0 34.0 41.0 16-17 38.65759538345035 40.0 38.0 41.0 34.5 41.0 18-19 38.754141619064534 40.0 38.0 41.0 35.0 41.0 20-21 38.72133588250142 40.0 38.0 41.0 35.0 41.0 22-23 38.590006591064196 40.0 38.0 41.0 34.0 41.0 24-25 38.4192536204108 40.0 38.0 41.0 34.0 41.0 26-27 38.163236352757494 40.0 37.5 41.0 34.0 41.0 28-29 38.094446210371814 40.0 37.0 41.0 34.0 41.0 30-31 38.06803520843656 40.0 37.0 41.0 34.0 41.0 32-33 37.89881197236428 40.0 36.5 41.0 33.5 41.0 34-35 37.70747010648542 40.0 36.0 41.0 33.0 41.0 36-37 37.48481250525883 40.0 36.0 41.0 33.0 41.0 38-39 37.2677725007713 40.0 35.0 41.0 33.0 41.0 40-41 37.01182067630863 40.0 35.0 41.0 32.5 41.0 42-43 36.69206407823266 39.5 35.0 41.0 31.5 41.0 44-45 36.196875321372815 39.0 35.0 41.0 30.5 41.0 46-47 35.577863373316006 39.0 35.0 41.0 28.0 41.0 48-49 35.03762048558848 39.0 35.0 41.0 24.5 41.0 50-51 34.72426329665398 39.0 35.0 41.0 23.0 41.0 52-53 34.442600478670194 38.5 35.0 40.0 22.5 41.0 54-55 34.21090821125062 38.0 34.0 40.0 20.5 41.0 56-57 33.94361367949665 38.0 34.0 40.0 19.0 41.0 58-59 33.670576507764366 38.0 34.0 40.0 17.0 41.0 60-61 33.313123463253646 37.5 33.5 40.0 12.0 41.0 62-63 32.938960201191065 37.0 33.0 40.0 7.5 41.0 64-65 32.566888783972026 36.0 33.0 39.5 4.5 41.0 66-67 32.15280517562148 36.0 32.5 39.0 2.0 40.5 68-69 31.69751923562353 35.0 32.0 39.0 2.0 40.0 70-71 31.284351831941887 35.0 31.0 38.0 2.0 40.0 72-73 30.849482998793974 35.0 31.0 37.5 2.0 39.5 74-75 30.394579200284213 35.0 31.0 37.0 2.0 39.0 76-77 29.102655824911416 33.5 29.0 35.5 2.0 38.0 78-79 29.651766264970128 34.0 30.0 36.0 2.0 38.0 80-81 29.469483606480743 34.0 30.0 35.5 2.0 37.0 82-83 29.127255452820133 34.0 30.0 35.0 2.0 37.0 84-85 28.798725961313725 34.0 29.5 35.0 2.0 36.5 86-87 28.458748585959633 34.0 29.0 35.0 2.0 36.0 88-89 28.097075624281295 34.0 29.0 35.0 2.0 36.0 90-91 27.649482765068296 34.0 28.0 35.0 2.0 35.0 92-93 27.248820853940146 34.0 27.0 35.0 2.0 35.0 94-95 26.8555622037527 33.0 26.0 35.0 2.0 35.0 96-97 26.450641810719596 33.0 25.0 35.0 2.0 35.0 98-99 25.87248277441732 33.0 24.0 35.0 2.0 35.0 100-101 24.490896384731165 31.5 17.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0897528120795954 1101 2 0.14965471702476663 1101 3 0.30408336587308327 1101 4 0.19453184017923775 1101 5 -0.022308417625993116 1101 6 0.050054135185689574 1101 7 0.17150059133867046 1101 8 0.01750547982091888 1101 9 0.16876875215430687 1101 10-11 0.12856498372187275 1101 12-13 0.11296390009422907 1101 14-15 0.2572348894522918 1101 16-17 0.15639385676975337 1101 18-19 0.03920732431711116 1101 20-21 0.006328025724748443 1101 22-23 -0.009627738000084207 1101 24-25 0.07528885791509055 1101 26-27 -0.04917102479626578 1101 28-29 0.12034083119533534 1101 30-31 0.24419137462597007 1101 32-33 0.274501648149176 1101 34-35 0.47286569655840793 1101 36-37 0.3936038711662988 1101 38-39 0.5571782671768304 1101 40-41 0.731728493565079 1101 42-43 0.7516933131892358 1101 44-45 0.948421626383606 1101 46-47 1.1003511433334268 1101 48-49 1.1024605768833098 1101 50-51 1.1553822262912874 1101 52-53 1.3883085630210772 1101 54-55 1.3388695168060067 1101 56-57 1.4174064900249022 1101 58-59 1.5379596436292928 1101 60-61 1.7027575314602927 1101 62-63 1.6470822491590482 1101 64-65 1.8199521392211153 1101 66-67 1.6437480324647709 1101 68-69 1.459779907698266 1101 70-71 1.3432428797476241 1101 72-73 1.7571756663695197 1101 74-75 1.768786037520826 1101 76-77 1.415026254166957 1101 78-79 1.3148426434799703 1101 80-81 1.312452202901646 1101 82-83 1.3671282322849976 1101 84-85 1.4768947449667778 1101 86-87 1.2169564410360252 1101 88-89 1.0950912521894445 1101 90-91 0.9929596052869378 1101 92-93 1.254705921413418 1101 94-95 1.112447237245199 1101 96-97 1.0517104767088803 1101 98-99 1.360539457575328 1101 100-101 1.2519544919215377 1102 1 -0.15984146785548958 1102 2 -0.10610572389690986 1102 3 -0.09729137834688117 1102 4 -0.09536592620440132 1102 5 -0.27872937865334535 1102 6 -0.18788598288472969 1102 7 0.04166233056454871 1102 8 -0.11687802168044215 1102 9 -0.1369932567676173 1102 10-11 -0.14555968013807785 1102 12-13 -0.21604077254651344 1102 14-15 4.2486836269262085E-4 1102 16-17 -0.0018154085437203094 1102 18-19 -0.08815251606045393 1102 20-21 -0.14690577879265732 1102 22-23 -0.15366522104608293 1102 24-25 -0.1863447239462772 1102 26-27 -0.17202776445333967 1102 28-29 -0.4002185920941699 1102 30-31 -0.1711444518277574 1102 32-33 0.06341255437492777 1102 34-35 -0.1675791059127576 1102 36-37 0.05466003693528165 1102 38-39 0.08848045218517342 1102 40-41 0.09412471154848134 1102 42-43 0.03713011885912465 1102 44-45 -0.09521190059775364 1102 46-47 0.08429482076441275 1102 48-49 0.4545040732102734 1102 50-51 0.3579502187021717 1102 52-53 0.1371845028412224 1102 54-55 0.1664159492134374 1102 56-57 0.3204433145107899 1102 58-59 0.2744905214742346 1102 60-61 0.3146323893602698 1102 62-63 0.4131042717225739 1102 64-65 0.41547569450489164 1102 66-67 0.4149042758497714 1102 68-69 0.45612058711277825 1102 70-71 0.4196907319792835 1102 72-73 0.666981351938432 1102 74-75 0.5261115386929056 1102 76-77 0.16569755427436306 1102 78-79 0.4573836662865318 1102 80-81 0.7532082534226134 1102 82-83 0.766695856215815 1102 84-85 0.635725330379671 1102 86-87 0.762869448382105 1102 88-89 0.7710581203441471 1102 90-91 0.5409159129599459 1102 92-93 0.6713618080783625 1102 94-95 0.5338786646742264 1102 96-97 0.6961811198241854 1102 98-99 0.6130413979756391 1102 100-101 0.6313605726265124 1103 1 0.20775194673657893 1103 2 0.18910794843991852 1103 3 0.08800000449622303 1103 4 0.1883342214750101 1103 5 0.10101498028757305 1103 6 0.028805156771191776 1103 7 0.025297684427144418 1103 8 0.07685759430259509 1103 9 0.01582338093817981 1103 10-11 0.007042380641891555 1103 12-13 0.034484099443616856 1103 14-15 0.029026710358337482 1103 16-17 -0.027355321955624845 1103 18-19 -0.023255618169621073 1103 20-21 0.05146007065848579 1103 22-23 0.1131327863336864 1103 24-25 0.11266085926118308 1103 26-27 -0.019701287764391395 1103 28-29 -0.08342942023488575 1103 30-31 0.21876391120274263 1103 32-33 0.26616671232878275 1103 34-35 0.4562338839852913 1103 36-37 0.3417734303221067 1103 38-39 0.40199942962094326 1103 40-41 0.7794665830800085 1103 42-43 0.7797608740436885 1103 44-45 0.8043860832206136 1103 46-47 0.43426137200832926 1103 48-49 0.42090279921237084 1103 50-51 0.4825499906615889 1103 52-53 0.9175639674086824 1103 54-55 0.7212529759345756 1103 56-57 0.7402315144628275 1103 58-59 0.7877753576173987 1103 60-61 0.7160100994458105 1103 62-63 0.8226527345523778 1103 64-65 0.9342795754020727 1103 66-67 0.8461902227440845 1103 68-69 1.0347682896670882 1103 70-71 0.9724514113887359 1103 72-73 1.167386313293548 1103 74-75 1.0550362979249712 1103 76-77 0.85578431067065 1103 78-79 1.2894993106273844 1103 80-81 1.2295174882244666 1103 82-83 1.215889509787683 1103 84-85 1.0380696884791085 1103 86-87 1.1511863658793793 1103 88-89 1.3433084853352888 1103 90-91 1.414672103083518 1103 92-93 1.1408792624386912 1103 94-95 0.9683075324233137 1103 96-97 1.1870184747217962 1103 98-99 0.9585575817039746 1103 100-101 1.248699440081083 1104 1 0.12104938435977175 1104 2 0.2273993767366349 1104 3 0.1807599679595171 1104 4 0.09853084663726008 1104 5 0.021283236621400192 1104 6 0.05079929166855379 1104 7 0.0035919972005657996 1104 8 0.1465417441030823 1104 9 -0.012925236916736083 1104 10-11 0.08677412430805731 1104 12-13 0.11339857470922965 1104 14-15 0.04753543590038589 1104 16-17 0.13416335503106325 1104 18-19 0.1643315170642623 1104 20-21 -0.017454885353245686 1104 22-23 0.05302750293384406 1104 24-25 -0.0761641972264897 1104 26-27 -0.13933495922249506 1104 28-29 -0.053094340190654066 1104 30-31 0.1142857611138055 1104 32-33 0.19607392832801196 1104 34-35 0.03365804628517566 1104 36-37 0.3256083421051912 1104 38-39 0.1780799065210914 1104 40-41 0.1326226813445075 1104 42-43 0.2875850171137273 1104 44-45 0.40768640532051137 1104 46-47 0.5976189028962651 1104 48-49 0.6511441337635944 1104 50-51 0.6127841140210464 1104 52-53 0.5701888412128255 1104 54-55 0.7601934114905546 1104 56-57 0.49651726995535483 1104 58-59 0.44990698590449796 1104 60-61 0.4917540540633709 1104 62-63 0.5532546286920805 1104 64-65 0.6457718113522688 1104 66-67 0.4526153946108238 1104 68-69 0.7035702703410927 1104 70-71 0.5983347823805154 1104 72-73 0.6979978223556103 1104 74-75 0.8668362113906696 1104 76-77 0.7491668403567218 1104 78-79 0.8417924696101231 1104 80-81 0.7228471358375614 1104 82-83 0.722692067357027 1104 84-85 0.7764476510770599 1104 86-87 0.6937323973201757 1104 88-89 0.8587524543752387 1104 90-91 0.8074777374280231 1104 92-93 0.8248127271759635 1104 94-95 0.8926260748010861 1104 96-97 0.8982703376130026 1104 98-99 1.080919487381589 1104 100-101 1.3678884213800586 1105 1 -0.04637677683187036 1105 2 -0.06872505047966371 1105 3 -0.02011853231500993 1105 4 -0.14242669842003863 1105 5 -0.18382835973403644 1105 6 0.0023350224912093154 1105 7 0.08587802089882501 1105 8 -0.03619997631015792 1105 9 -0.06026860856637484 1105 10-11 -0.05160864575107382 1105 12-13 -0.07618112667152133 1105 14-15 -0.23752677011704293 1105 16-17 -0.045297905687675666 1105 18-19 -0.18786435408565438 1105 20-21 -0.13985031674697979 1105 22-23 0.0169373858089088 1105 24-25 -0.06047542720278187 1105 26-27 0.17870896073074505 1105 28-29 -0.013356878175926568 1105 30-31 -0.2502513689330783 1105 32-33 -0.2403157695356981 1105 34-35 -0.08783155821257793 1105 36-37 -0.08958171707718776 1105 38-39 0.06981082026764796 1105 40-41 0.32553850775974524 1105 42-43 0.19267181709045644 1105 44-45 0.43006134174816424 1105 46-47 0.42415178719586066 1105 48-49 0.3305725013268912 1105 50-51 0.5259208812311229 1105 52-53 0.44970627332008917 1105 54-55 0.36857509354532425 1105 56-57 0.3485043960803651 1105 58-59 0.3478926596701868 1105 60-61 0.5218057469222686 1105 62-63 0.26581525963340624 1105 64-65 0.20619653789628956 1105 66-67 0.269210750603321 1105 68-69 0.651182859816803 1105 70-71 0.400295214444764 1105 72-73 0.7875051587943958 1105 74-75 0.8373715348442516 1105 76-77 0.7494021529302586 1105 78-79 0.4069942944211782 1105 80-81 0.6573998774820673 1105 82-83 0.6285303494234249 1105 84-85 0.4855924543380361 1105 86-87 0.7136188400266263 1105 88-89 0.6447053058322787 1105 90-91 0.36416276522273705 1105 92-93 0.41044246663686934 1105 94-95 0.3457963424248547 1105 96-97 0.4208122700605834 1105 98-99 0.6743702071091313 1105 100-101 0.601107219059319 1106 1 -0.3370210096417168 1106 2 -0.26208110947796825 1106 3 -0.31041458494659224 1106 4 -0.14491701569507853 1106 5 -0.3682676901669737 1106 6 -0.2569066742405397 1106 7 -0.26233863338810437 1106 8 -0.2459636117091364 1106 9 -0.5151345094577167 1106 10-11 -0.37195264776720194 1106 12-13 -0.46067318153145465 1106 14-15 -0.366607110206175 1106 16-17 -0.4021892204160338 1106 18-19 -0.3725872026637589 1106 20-21 -0.6543511921714256 1106 22-23 -0.3633781649126533 1106 24-25 -0.19376211544556554 1106 26-27 -0.13699761291101709 1106 28-29 -0.2684751267964671 1106 30-31 -0.37983739928112925 1106 32-33 -0.2710285772606582 1106 34-35 -0.09316700494432695 1106 36-37 -0.1250148532189499 1106 38-39 -0.01188551356087686 1106 40-41 -0.16309092241852596 1106 42-43 -0.09825759186540495 1106 44-45 0.29994143512677596 1106 46-47 0.13413593670774304 1106 48-49 -0.17628664818499118 1106 50-51 -0.05992045471963792 1106 52-53 0.3175851596740813 1106 54-55 0.10440913429234655 1106 56-57 0.06942471919156645 1106 58-59 0.11480924233147505 1106 60-61 0.08179719163788945 1106 62-63 -0.11258198410597942 1106 64-65 -0.17990377778707511 1106 66-67 -0.011157192945063343 1106 68-69 0.21139150264362527 1106 70-71 0.1692760081629281 1106 72-73 -0.14114443267419574 1106 74-75 -0.07129251439174311 1106 76-77 -0.017148011543426378 1106 78-79 0.06552570358925891 1106 80-81 -0.11971672640163433 1106 82-83 -0.206967780351615 1106 84-85 -0.2358517970414198 1106 86-87 -0.15621317132736579 1106 88-89 -0.006177745419844172 1106 90-91 -0.029159802158417847 1106 92-93 -0.05012032517128162 1106 94-95 0.2551534360156893 1106 96-97 0.4232578213127063 1106 98-99 0.13111130696057316 1106 100-101 0.04928308652536373 1107 1 -0.0771155636126366 1107 2 -0.00410257200593378 1107 3 -0.032834886960017684 1107 4 -0.027375593715213142 1107 5 -0.060770661363839906 1107 6 -0.12959525785351644 1107 7 0.023739561522411634 1107 8 -0.034562954550303004 1107 9 -0.12577226812661735 1107 10-11 -0.08610384673252724 1107 12-13 -0.03229868838268857 1107 14-15 0.0409340496092625 1107 16-17 0.06611073064660644 1107 18-19 -0.08779142875436463 1107 20-21 0.1184003109046401 1107 22-23 -0.08967413107595945 1107 24-25 0.0799419183911283 1107 26-27 0.2122622095162754 1107 28-29 0.005829700524564885 1107 30-31 0.152028687128265 1107 32-33 0.010404937840014838 1107 34-35 -0.0553323083559647 1107 36-37 -0.033657103638411456 1107 38-39 -0.20645084493671817 1107 40-41 -0.25400517717243787 1107 42-43 0.08426565338068315 1107 44-45 0.11827896309097952 1107 46-47 -0.2051463444723467 1107 48-49 -0.0792792551480801 1107 50-51 -0.04409883139749127 1107 52-53 0.06219549594365503 1107 54-55 0.1838973893258995 1107 56-57 0.2759076085931369 1107 58-59 0.31812431154288845 1107 60-61 0.2758403788582484 1107 62-63 0.5310032892441328 1107 64-65 0.20236036161449533 1107 66-67 0.487872798665677 1107 68-69 0.2889965431917254 1107 70-71 0.16177810547836557 1107 72-73 0.33537000384705706 1107 74-75 0.32352711648074006 1107 76-77 0.3975830077518765 1107 78-79 0.5607717886004444 1107 80-81 0.6936593397337134 1107 82-83 0.5265792169917844 1107 84-85 0.5771441306711083 1107 86-87 0.6604137337341527 1107 88-89 0.6064328005812776 1107 90-91 0.6696154530149592 1107 92-93 0.7273239211933706 1107 94-95 0.3075732137002518 1107 96-97 1.091075826765728 1107 98-99 0.8588295151540848 1107 100-101 0.7952348307927011 1108 1 -0.13760484700731013 1108 2 -0.0424658541016214 1108 3 -0.1799374344089557 1108 4 0.00573402597159145 1108 5 -0.1293858017261158 1108 6 -0.07666893902548821 1108 7 -0.07450681215154731 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2316 58-59 -0.5014634540618061 2316 60-61 -0.3336201440834543 2316 62-63 -0.7203473929498543 2316 64-65 -0.5932290309985362 2316 66-67 -0.22282035994111027 2316 68-69 -0.33329877650657025 2316 70-71 -0.04240259855643913 2316 72-73 0.025380703195367005 2316 74-75 -0.21772285036012562 2316 76-77 -0.3203918974129891 2316 78-79 0.036375505960801746 2316 80-81 -0.612602873881869 2316 82-83 -0.5370111659741461 2316 84-85 -0.13539709718963877 2316 86-87 0.3345345744730288 2316 88-89 0.29648329089438263 2316 90-91 0.22427939621114845 2316 92-93 0.14030550239789008 2316 94-95 0.4327025343358457 2316 96-97 0.3243702533779249 2316 98-99 0.1209635488084011 2316 100-101 -0.29455569188372976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 10.0 6 30.0 7 56.0 8 159.0 9 291.0 10 582.0 11 935.0 12 1495.0 13 2180.0 14 2763.0 15 4004.0 16 5710.0 17 8520.0 18 7196.0 19 3698.0 20 3052.0 21 2918.0 22 3028.0 23 3059.0 24 3431.0 25 3880.0 26 4152.0 27 5109.0 28 6006.0 29 7235.0 30 9157.0 31 10924.0 32 13960.0 33 18215.0 34 25404.0 35 38583.0 36 57430.0 37 87357.0 38 78879.0 39 8440.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.25343984136041 19.803056805320413 15.351373598106802 19.592129755212376 2 15.72646616119593 22.80274487439582 39.131054663762235 22.339734300646015 3 19.27956396136982 27.658629619588083 31.136467750530556 21.92533866851154 4 12.41807914886456 18.215410936492056 37.10512046221591 32.26138945242747 5 12.508327119646392 39.56135655499171 32.71570453860161 15.214611786760287 6 29.11777904509036 37.86916971289138 17.312762357076746 15.700288884941521 7 26.618784065517982 36.46821798191898 19.89122406813571 17.021773884427326 8 27.32954386096127 33.477931621214815 19.48454138346905 19.70798313435487 9 28.189420640782327 16.395622785449174 20.217972569954096 35.1969840038144 10-11 24.739863317221843 28.23873675944018 25.939927825509756 21.08147209782822 12-13 27.06028368379903 26.385608487374235 27.274384664199207 19.279723164627526 14-15 23.365556313865543 26.590970709497675 28.00886755233118 22.0346054243056 16-17 20.106251694511187 30.384689098099344 28.15179080616662 21.357268401222854 18-19 19.96438020623954 27.59026485794153 33.187176874246234 19.258178061572693 20-21 21.482779091835493 26.025237699017417 33.27832988977497 19.21365331937212 22-23 20.829807921541864 26.65865755435367 33.050560248313644 19.46097427579082 24-25 20.937779741078085 26.867414123140996 33.188656798745356 19.00614933703556 26-27 20.528510391638076 27.025154028103703 33.36484793522873 19.081487645029497 28-29 19.891361637428815 27.54571494350127 33.492543383472615 19.070380035597296 30-31 19.974734017688526 27.475996148164768 33.14436435369571 19.404905480451003 32-33 19.78686555163935 27.70888064096931 32.957891981339344 19.546361826052 34-35 20.300711460972487 27.438927479595748 32.84535306601348 19.415007993418286 36-37 20.590204778994583 27.33343227732052 32.5935517346556 19.4828112090293 38-39 20.30384480542246 27.490943087530678 32.755755521795024 19.44945658525184 40-41 20.03017398539682 27.970887129194207 32.64212858652058 19.356810298888398 42-43 20.085296269544628 28.403727884096185 31.756205543205635 19.754770303153553 44-45 20.225802380262333 28.48800519805914 31.25146078550527 20.034731636173255 46-47 20.617176032833783 28.82433645279209 30.254971345231528 20.3035161691426 48-49 20.976646129970177 29.156110056748595 29.56080607312809 20.30643774015314 50-51 20.900568420855812 29.549002926245528 29.29716350513729 20.253265147761375 52-53 21.054593644531288 29.927404803530194 28.761464244645346 20.256537307293176 54-55 20.135817993137813 29.67638342230491 29.11976571337752 21.068032871179753 56-57 20.78966558529585 30.26642390359283 28.435650645550332 20.508259865560987 58-59 20.74549143161654 29.320068621859896 28.906491029608368 21.027948916915197 60-61 20.59053130521769 29.18813047502407 29.81393098548096 20.407407234277272 62-63 20.789198133934164 29.357114142273495 29.633845348391503 20.21984237540084 64-65 20.542033226442786 29.330469414657408 29.61070650598805 20.516790852911754 66-67 20.217738844273253 29.51815113637426 29.24469208978806 21.01941792956443 68-69 20.418041752755624 29.583477464169857 29.163472415695146 20.835008367379373 70-71 20.325953834503522 29.982447201368696 28.812065854547836 20.87953310957995 72-73 20.310177351046622 29.54830174920299 28.74019520768864 21.40132569206174 74-75 20.271028299505435 29.94820638912521 28.491394220431367 21.28937109093799 76-77 20.20885726840122 29.43552910819629 29.009564054860093 21.346049568542394 78-79 19.930139393996054 29.412507128633266 28.812883894430787 21.844469582939897 80-81 19.809069491319427 29.77957331039705 28.858460402195153 21.552896796088366 82-83 19.899357368987534 29.869428690629086 28.568974443175826 21.66223949720755 84-85 19.603975206379776 29.69543206529361 28.327903106681752 22.372689621644867 86-87 19.713826276375944 29.224124229873883 28.258837167992674 22.803212325757507 88-89 19.753209053597974 28.95288557725569 28.364364312893244 22.929541056253097 90-91 19.445765756500176 29.011922195432376 28.753750156317388 22.788561891750064 92-93 19.395549301963573 29.06171188964176 28.449805185547035 23.09293362284763 94-95 19.37842992436637 29.213957162757215 28.10773351532773 23.299879397548683 96-97 19.612038742368856 29.192454400119665 27.894341968718155 23.30116488879332 98-99 19.555774222236767 29.426627135262613 27.115746221474808 23.901852421025815 100-101 19.161618558183076 29.501134290399378 27.1873221279545 24.14992502346304 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1085.0 1 706.5 2 409.0 3 473.5 4 417.0 5 485.0 6 833.0 7 1544.0 8 2141.5 9 2300.5 10 2071.0 11 1806.0 12 1855.5 13 1970.0 14 1979.0 15 1889.0 16 1857.0 17 1861.5 18 1859.0 19 1910.0 20 1959.0 21 2080.0 22 2299.0 23 2577.5 24 2849.0 25 3157.0 26 3626.0 27 4247.5 28 4774.0 29 5203.0 30 5802.5 31 6465.5 32 7233.0 33 7938.0 34 8953.5 35 9593.5 36 10270.0 37 11334.0 38 11553.5 39 11340.0 40 11372.0 41 12071.0 42 12829.0 43 13576.5 44 14424.5 45 14790.0 46 14664.0 47 15331.5 48 19235.5 49 18862.5 50 14903.0 51 13319.5 52 11923.5 53 11402.0 54 10851.5 55 9957.0 56 8918.0 57 8052.0 58 7439.5 59 6744.0 60 5950.5 61 5041.0 62 3985.0 63 2810.5 64 1875.5 65 1404.5 66 1001.0 67 765.5 68 543.5 69 356.0 70 285.5 71 221.5 72 194.5 73 166.0 74 127.0 75 98.5 76 71.5 77 47.5 78 34.0 79 20.0 80 11.0 81 5.5 82 1.0 83 1.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050484747062068194 2 0.0 3 0.0 4 0.0 5 0.007712947467815973 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0038564737339079865 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014023540850574498 24-25 2.3372568084290829E-4 26-27 0.0018698054467432663 28-29 0.0024541196488505374 30-31 9.349027233716331E-4 32-33 0.0 34-35 0.0 36-37 1.1686284042145414E-4 38-39 4.6745136168581657E-4 40-41 0.0 42-43 0.011452558361302505 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.5058852126436244E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.008530987350766153 92-93 0.0021035311275861748 94-95 0.0 96-97 0.0 98-99 0.001519216925478904 100-101 0.012036872563409778 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 427852.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.84800271740416 #Duplication Level Percentage of deduplicated Percentage of total 1 78.97419271976612 43.31576736898531 2 11.685321262879933 12.818330647603581 3 3.9126017259496617 6.437951702910216 4 1.765262989321947 3.8728459694105246 5 0.9403699811373432 2.5788707640393147 6 0.5635462707402901 1.8545632433367845 7 0.3980384386869608 1.5282129356713612 8 0.25484728305024623 1.1182291578610375 9 0.2002817178774022 0.9886546985747516 >10 1.1560786559514675 11.966278174039715 >50 0.09095644953782153 3.5031538191782943 >100 0.05423224925403605 5.248758246091802 >500 0.0017081023387097972 0.7797017492744135 >1k 0.0021351279233872466 2.745702495267032 >5k 4.270255846774493E-4 1.2429790277558763 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5307 1.2403821882333144 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4421 1.0333012350064976 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3268 0.7638155249946244 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1880 0.4394042799846676 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1078 0.25195628394865516 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1076 0.2514888325869693 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 956 0.22344175088582033 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 858 0.20053663416321532 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 822 0.19212250965287062 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 693 0.16197189682413546 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 466 0.10891616727279527 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 446 0.1042416536559371 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.1686284042145414E-4 0.0 22-23 0.0 0.0 0.0 9.349027233716333E-4 0.0 24-25 0.0 0.0 0.0 0.0025709824892719913 0.0 26-27 0.0 0.0 0.0 0.0029215710105363535 0.0 28-29 0.0 0.0 0.0 0.0042070622551723495 0.0 30-31 0.0 0.0 0.0 0.007829810308237428 0.0 32-33 0.0 0.0 0.0 0.01414040369099595 0.0 34-35 0.0 0.0 0.0 0.019749820031225752 0.0 36-37 0.0 0.0 0.0 0.02454119648850537 0.0 38-39 0.0 0.0 0.0 0.030618064190420985 0.0 40-41 0.0 0.0 0.0 0.03879846301992278 0.0 42-43 0.0 0.0 0.0 0.0563278890831409 0.0 44-45 0.0 0.0 0.0 0.08671222759271897 0.0 46-47 0.0 0.0 0.0 0.12188794255957668 0.0 48-49 0.0 0.0 0.0 0.19971859428026514 0.0 50-51 0.0 0.0 0.0 0.27240728102240963 0.0 52-53 0.0 0.0 0.0 0.3578340173704926 0.0 54-55 0.0 0.0 0.0 0.5233117994072717 0.0 56-57 0.0 0.0 0.0 0.6766358460402195 0.0 58-59 0.0 0.0 0.0 0.8896768041285305 0.0 60-61 0.0 0.0 0.0 1.1166244402269943 0.0 62-63 0.0 0.0 0.0 1.4257266531417405 0.0 64-65 0.0 0.0 0.0 1.6702037153034226 0.0 66-67 0.0 0.0 0.0 2.0038471247066743 0.0 68-69 0.0 0.0 0.0 2.4281760982769742 0.0 70-71 0.0 0.0 0.0 2.7156586857137515 0.0 72-73 0.0 0.0 0.0 3.2153641913558895 0.0 74-75 0.0 0.0 0.0 3.847475295195535 0.0 76-77 0.0 0.0 0.0 4.313056851434608 0.0 78-79 0.0 0.0 0.0 4.748721520525789 0.0 80-81 0.0 0.0 0.0 5.410749511513327 0.0 82-83 0.0 0.0 0.0 6.1899909314435835 0.0 84-85 0.0 0.0 0.0 6.950066845544721 0.0 86-87 0.0 0.0 0.0 8.04296812916616 0.0 88-89 0.0 0.0 0.0 9.378827258023803 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGGAC 90 2.4086186E-5 31.668262 3 GCTTATA 230 0.0 30.99069 1 ATAGGAG 210 1.8189894E-12 29.406244 5 CCGCCGA 35 0.008383549 27.147398 90-91 AGCGTCG 35 0.008393114 27.141052 52-53 GTGCTAT 90 8.917417E-4 26.399477 1 TTATAGG 235 5.456968E-12 26.277922 3 GTATGTA 145 7.8406447E-7 26.217411 1 GGTATCA 4975 0.0 25.598207 1 GTACATG 3410 0.0 25.501429 1 CTTATAG 320 0.0 25.235647 2 TATAGGA 290 0.0 24.570204 4 TACATGG 3540 0.0 24.287949 2 GAGTACT 3935 0.0 23.054379 12-13 ACATGGG 3565 0.0 22.918407 3 GTATCAA 6580 0.0 22.892921 1 AGTACTT 3965 0.0 21.92162 12-13 CATGGGG 945 0.0 21.614847 4 TCCTACG 110 0.0028677618 21.599571 1 ACGTCTA 155 3.8975344E-5 21.452694 5 >>END_MODULE