FastQCFastQC Report
Thu 26 May 2016
SRR937417_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937417_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488451
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79611.629846187232701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57911.1855846338732032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42940.8791055807030798No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14860.3042270360793611No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.24935971059533096No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10590.21680782719249217No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9210.18855524914474533No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8600.1760667907323355No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT8110.16603507823712102No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA7680.15723173870050425No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6990.14310544967663083No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.1416723478916002No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6440.13184536422281867No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6380.13061699126422097No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.11505760045531692No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5270.10789209153016371No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5180.1060495320922672No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA250.005010545357.0258524
GGTATCA42100.034.096081
GTATCAA63350.032.412821
GAATAGT600.00393602831.6875291
AGGTAAG600.00394196431.6777762
GTACATG33800.027.5625251
TACATGG34850.026.4511742
TATCAAC77650.026.3131562
ATCAACG77850.026.245563
GAGTACT48300.026.108712-13
TCAACGC78850.025.9153614
CAACGCA79500.025.7061125
AACGCAG80550.025.2964526
ACATGGG35200.025.243233
GTACTTT52150.024.2267514-15
AGTACTT48450.024.01818312-13
CATGGGA24550.024.0027354
ACGCAGA86950.023.2706137
CGCAGAG86900.023.174698
ATGGGAG7450.022.9657865
Produced by FastQC (version 0.11.5)