##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937417_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 488451 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.844733658033253 33.0 31.0 34.0 30.0 34.0 2 31.89830914462249 33.0 31.0 34.0 30.0 34.0 3 31.90333523731142 33.0 31.0 34.0 30.0 34.0 4 35.58076040380714 37.0 35.0 37.0 33.0 37.0 5 35.57666173270195 37.0 35.0 37.0 33.0 37.0 6 35.67106219457018 37.0 35.0 37.0 33.0 37.0 7 35.62483852013815 37.0 35.0 37.0 33.0 37.0 8 35.61990864999765 37.0 35.0 37.0 33.0 37.0 9 37.242484916603715 39.0 37.0 39.0 34.0 39.0 10-11 37.158166325793175 39.0 37.0 39.0 34.0 39.0 12-13 37.041444279978954 39.0 37.0 39.0 33.0 39.0 14-15 38.10968039782905 40.0 38.0 41.0 33.0 41.0 16-17 38.10664017475652 40.0 38.0 41.0 33.0 41.0 18-19 38.17410037035445 40.0 38.0 41.0 33.5 41.0 20-21 38.16963114007341 40.0 38.0 41.0 34.0 41.0 22-23 38.040074644130115 40.0 38.0 41.0 33.5 41.0 24-25 37.885203428798384 40.0 37.5 41.0 33.0 41.0 26-27 37.70059023320661 40.0 37.0 41.0 33.0 41.0 28-29 37.49264921148692 39.5 37.0 41.0 33.0 41.0 30-31 37.289889876364256 39.0 36.5 41.0 32.5 41.0 32-33 36.98433415020135 39.0 35.0 41.0 32.0 41.0 34-35 36.705902946252536 39.0 35.0 41.0 31.0 41.0 36-37 36.60744168811201 39.0 35.0 41.0 31.0 41.0 38-39 36.51479063406565 39.0 35.0 41.0 31.0 41.0 40-41 36.254799355513654 39.0 35.0 41.0 30.5 41.0 42-43 35.87346325424659 39.0 35.0 41.0 30.0 41.0 44-45 35.41941361569533 39.0 35.0 41.0 27.5 41.0 46-47 34.77318195683907 39.0 35.0 41.0 23.5 41.0 48-49 34.214563999254786 38.5 34.5 40.0 18.5 41.0 50-51 33.16747125095455 37.5 33.0 39.5 13.0 40.5 52-53 33.24106205126 38.0 33.5 39.5 9.0 40.5 54-55 33.55198371996372 38.0 34.0 40.0 7.5 41.0 56-57 33.31523939965319 38.0 34.0 40.0 3.5 41.0 58-59 33.07000292762222 38.0 33.0 40.0 2.0 41.0 60-61 32.78134756608135 37.5 33.0 40.0 2.0 41.0 62-63 32.452838667542906 37.0 33.0 40.0 2.0 41.0 64-65 32.07812554381095 36.0 32.0 40.0 2.0 41.0 66-67 31.67196402505062 36.0 32.0 39.0 2.0 41.0 68-69 31.190779627843938 35.0 31.0 39.0 2.0 40.5 70-71 30.738732237215196 35.0 31.0 38.5 2.0 40.0 72-73 30.324057070207658 35.0 30.5 37.5 2.0 40.0 74-75 29.879478187167187 35.0 30.0 37.0 2.0 39.0 76-77 29.443450827206824 35.0 29.5 36.5 2.0 39.0 78-79 28.990665389158792 34.5 29.0 36.0 2.0 39.0 80-81 28.55129173653038 34.0 29.0 36.0 2.0 37.5 82-83 28.1370894931119 34.0 28.0 35.0 2.0 37.0 84-85 27.696045253259793 34.0 27.0 35.0 2.0 36.5 86-87 27.28767675775052 34.0 26.0 35.0 2.0 36.0 88-89 26.915839050385813 34.0 25.5 35.0 2.0 36.0 90-91 26.48438942698449 33.5 25.0 35.0 2.0 35.0 92-93 26.078217671782838 33.0 23.5 35.0 2.0 35.0 94-95 25.590974324957877 33.0 20.5 35.0 2.0 35.0 96-97 25.035929909038984 33.0 16.5 35.0 2.0 35.0 98-99 24.409110637505094 32.0 2.0 35.0 2.0 35.0 100-101 22.81156349357459 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03521939857686007 1101 2 0.0755120041798989 1101 3 0.051896352619579034 1101 4 -0.014200689153724966 1101 5 0.1345767240344884 1101 6 0.06587041634989532 1101 7 0.018613033799653067 1101 8 0.09408080883073211 1101 9 -0.00423694803102137 1101 10-11 -0.04828775198092927 1101 12-13 0.11065933916112414 1101 14-15 -0.04020721203169586 1101 16-17 -0.09893021813413583 1101 18-19 0.06930640288936729 1101 20-21 0.12496661518282792 1101 22-23 0.04918911101155743 1101 24-25 0.014340288407133528 1101 26-27 0.1906416746193358 1101 28-29 0.09760366195354919 1101 30-31 0.3003346344407518 1101 32-33 0.1399091328017903 1101 34-35 0.10327143019863172 1101 36-37 0.23632939465171177 1101 38-39 0.3822721792371482 1101 40-41 0.4124081010125238 1101 42-43 0.6974115575988549 1101 44-45 0.7103469078857074 1101 46-47 0.9377993430920029 1101 48-49 1.2274635007317087 1101 50-51 1.1781524391035774 1101 52-53 1.2871005898185501 1101 54-55 1.481006547685844 1101 56-57 1.5216837893563877 1101 58-59 1.4989043910570174 1101 60-61 1.6352756246832314 1101 62-63 1.8573324772725357 1101 64-65 1.8912701120998534 1101 66-67 2.065203966701503 1101 68-69 1.8123093788652334 1101 70-71 2.0568625415119826 1101 72-73 1.8463641854344495 1101 74-75 1.803349271080343 1101 76-77 1.77108551473356 1101 78-79 1.8919555580124054 1101 80-81 2.0297658163009693 1101 82-83 1.8803539181510978 1101 84-85 1.9544224860324668 1101 86-87 2.0915585184966616 1101 88-89 1.9193153996111363 1101 90-91 2.2152740518639185 1101 92-93 2.210679573025928 1101 94-95 2.0980624738766096 1101 96-97 1.970710646892325 1101 98-99 2.1361504688827537 1101 100-101 2.3341284848708668 1102 1 -0.03512526102610991 1102 2 -0.11911309223547661 1102 3 -0.02068920334193436 1102 4 -0.04111580302536311 1102 5 0.034345327127901726 1102 6 0.2011848319712115 1102 7 0.08251917596665947 1102 8 0.08247848674248104 1102 9 0.29182118536726875 1102 10-11 0.102721057339906 1102 12-13 0.03862182850468798 1102 14-15 0.2604301443601358 1102 16-17 0.41686160584183085 1102 18-19 0.13858744864099748 1102 20-21 0.11653889626917646 1102 22-23 0.10024257587576102 1102 24-25 0.3332255236606514 1102 26-27 0.46303237002793196 1102 28-29 0.42989367940980827 1102 30-31 0.17639114400109435 1102 32-33 0.25313935242586894 1102 34-35 0.34269821540546275 1102 36-37 0.4975115486727404 1102 38-39 0.6346206548592832 1102 40-41 0.878599795081449 1102 42-43 0.9559874517246172 1102 44-45 1.1057473869178978 1102 46-47 1.4274960913565238 1102 48-49 1.7846428942282344 1102 50-51 1.5299163330669643 1102 52-53 1.4626252924532253 1102 54-55 1.4764638610459713 1102 56-57 1.6549643479566356 1102 58-59 1.6767552415421463 1102 60-61 1.7171292356928518 1102 62-63 1.5736358101999102 1102 64-65 1.5182785966530972 1102 66-67 1.789545297436689 1102 68-69 1.88632797180966 1102 70-71 2.1156941901134516 1102 72-73 1.984012868095924 1102 74-75 1.9303049809005017 1102 76-77 1.8861628500154985 1102 78-79 1.9729525945081612 1102 80-81 1.9604969493338906 1102 82-83 1.9698273886987359 1102 84-85 1.8481057564420809 1102 86-87 1.8653091781808264 1102 88-89 2.044152000918327 1102 90-91 2.1896986042935858 1102 92-93 1.981641291046472 1102 94-95 1.862959157187614 1102 96-97 2.1022009241626556 1102 98-99 2.044740571590502 1102 100-101 1.889558192985998 1103 1 -0.09010565318297381 1103 2 -0.17061636020933335 1103 3 -0.08257155628310997 1103 4 -0.1664491363586933 1103 5 -0.369497810127001 1103 6 -0.02268444907453926 1103 7 -0.055258601811118524 1103 8 0.02697521876862652 1103 9 0.12698458406007518 1103 10-11 -0.020664563575131467 1103 12-13 -0.06945660286785937 1103 14-15 -0.05256331969214756 1103 16-17 0.15738448165882346 1103 18-19 0.04130640288936149 1103 20-21 -0.04918659392690472 1103 22-23 -0.004541737849727667 1103 24-25 -0.20683983581647425 1103 26-27 -0.2804970417367727 1103 28-29 -0.3922762552307191 1103 30-31 -0.30741277756752794 1103 32-33 -0.23161881750877455 1103 34-35 -0.11076583688211628 1103 36-37 -0.09027930100045722 1103 38-39 -0.09472575036947717 1103 40-41 0.14632528527753408 1103 42-43 0.30631424911023686 1103 44-45 0.007812746395025272 1103 46-47 0.21118236586632833 1103 48-49 0.2924076001105931 1103 50-51 0.4016679670538892 1103 52-53 0.5666906519303438 1103 54-55 0.5210520963400853 1103 56-57 0.6355264394599018 1103 58-59 0.8531735421957407 1103 60-61 0.6159919807908878 1103 62-63 0.7968645683698412 1103 64-65 0.511899511685769 1103 66-67 0.7730093497242763 1103 68-69 0.9085371221364582 1103 70-71 0.985040595342209 1103 72-73 1.2059997962004978 1103 74-75 1.3371546541031165 1103 76-77 1.387731477466481 1103 78-79 1.4848872350310387 1103 80-81 1.2763662303796437 1103 82-83 1.4065071272608272 1103 84-85 1.315896606115281 1103 86-87 1.5552438187036977 1103 88-89 1.3333153996111378 1103 90-91 1.5390711533131913 1103 92-93 1.4962033825497372 1103 94-95 1.6452467388869607 1103 96-97 1.4276257607639593 1103 98-99 1.2018647545970396 1103 100-101 1.3690353171690042 1104 1 0.18641752885704577 1104 2 0.15932019420745291 1104 3 0.10349188930009845 1104 4 -0.010759531673478762 1104 5 -0.04494729768190808 1104 6 0.058582510417899414 1104 7 0.03905081741777394 1104 8 0.03958429636745109 1104 9 0.0923628173446076 1104 10-11 0.0765897068006609 1104 12-13 0.07909781392081783 1104 14-15 0.15607699265533626 1104 16-17 0.09901454510440999 1104 18-19 0.19800332821878897 1104 20-21 0.13848934555576875 1104 22-23 -0.044045886214782115 1104 24-25 0.1016960353401899 1104 26-27 -0.011546334074495235 1104 28-29 -0.02349343434247686 1104 30-31 -0.038342011340098736 1104 32-33 -0.04296874430232833 1104 34-35 0.07324587614863276 1104 36-37 0.2912023973890001 1104 38-39 0.5766602916022165 1104 40-41 0.32312079528241355 1104 42-43 0.5558994126046244 1104 44-45 0.7021377829982498 1104 46-47 0.7041389300439747 1104 48-49 0.755558405381457 1104 50-51 0.6291086698357304 1104 52-53 0.694740432311022 1104 54-55 0.8619374062473639 1104 56-57 0.8928431793330134 1104 58-59 0.9077191742113584 1104 60-61 1.0461247284726838 1104 62-63 1.1778884824742804 1104 64-65 1.2467496825008055 1104 66-67 1.143819501017589 1104 68-69 1.0755288254063444 1104 70-71 1.1000088725506032 1104 72-73 1.1933536273376362 1104 74-75 1.13643039836861 1104 76-77 1.2342322095309015 1104 78-79 1.389739358017863 1104 80-81 1.2641046393596334 1104 82-83 1.2152938524926462 1104 84-85 1.281891725685803 1104 86-87 1.3431013101629468 1104 88-89 1.3109698652041075 1104 90-91 1.5366885276421343 1104 92-93 1.1671531141261156 1104 94-95 1.0644902723179008 1104 96-97 1.317049870085583 1104 98-99 1.1363596301460888 1104 100-101 1.356502374270029 1105 1 0.1091324420551203 1105 2 0.031983382390407655 1105 3 0.35051982080826605 1105 4 0.13211457020501172 1105 5 0.15044041381122497 1105 6 -0.004064114454202183 1105 7 -0.003109309661823545 1105 8 0.0782339060273145 1105 9 0.2992883163638069 1105 10-11 0.08094676203619144 1105 12-13 0.153482908071652 1105 14-15 0.22284465971582534 1105 16-17 0.2647847390590812 1105 18-19 0.3972986808816472 1105 20-21 0.056223959483652663 1105 22-23 0.11661398930599631 1105 24-25 0.1118474228707882 1105 26-27 0.1440074627677319 1105 28-29 0.12474819779373547 1105 30-31 0.250628406473659 1105 32-33 0.27631168442172793 1105 34-35 0.49402180790552563 1105 36-37 0.21916471444355778 1105 38-39 0.25779677215304986 1105 40-41 0.23543982839207445 1105 42-43 -0.18699334419735436 1105 44-45 -0.06341505283277371 1105 46-47 0.1337872564712157 1105 48-49 -0.021932168229177762 1105 50-51 -0.011204619818698802 1105 52-53 0.0486752079148971 1105 54-55 0.1794201787081704 1105 56-57 0.33953673547019747 1105 58-59 0.2360203890425865 1105 60-61 0.6057809125798244 1105 62-63 0.2055698450751251 1105 64-65 0.19958805737432073 1105 66-67 0.3784404951554201 1105 68-69 0.17194510154443776 1105 70-71 0.26800069830231266 1105 72-73 0.37469220289290206 1105 74-75 0.10268618563464571 1105 76-77 0.3179631376981398 1105 78-79 0.3419219840657881 1105 80-81 0.3664537464671618 1105 82-83 0.403729778483477 1105 84-85 0.19762376184243635 1105 86-87 -0.02891319545331683 1105 88-89 0.24846597876171472 1105 90-91 0.4798871121291519 1105 92-93 0.2873204996668548 1105 94-95 0.029017652657874038 1105 96-97 0.08385484699304868 1105 98-99 0.11298444571673016 1105 100-101 -0.06196854383485473 1106 1 0.16707786741627828 1106 2 0.12945854615770713 1106 3 0.2075108407206372 1106 4 0.3244272681356861 1106 5 0.43998533594041334 1106 6 0.3167462625359505 1106 7 0.436576604181397 1106 8 0.4044583648360387 1106 9 0.41005574510876386 1106 10-11 0.18136539897168547 1106 12-13 0.20621380911715903 1106 14-15 0.319092111019458 1106 16-17 0.17686013708953396 1106 18-19 0.23896932423767936 1106 20-21 0.35974224502440677 1106 22-23 0.3772859775060837 1106 24-25 0.02787926530712781 1106 26-27 0.25223704066023345 1106 28-29 0.4398885387767777 1106 30-31 0.37033254078453126 1106 32-33 0.32775196900807657 1106 34-35 0.29754876985946055 1106 36-37 0.008776878774817476 1106 38-39 0.06074241442453143 1106 40-41 0.08820543509026635 1106 42-43 0.07560263862334438 1106 44-45 0.3672734205523298 1106 46-47 0.48850071792625016 1106 48-49 0.49865479112182953 1106 50-51 0.5756679670538887 1106 52-53 0.6792075058629337 1106 54-55 0.9657786881003858 1106 56-57 0.7770470387108332 1106 58-59 0.6416079991245738 1106 60-61 0.43653879727029476 1106 62-63 0.6837334822275238 1106 64-65 0.8349856540078733 1106 66-67 0.760911971447122 1106 68-69 0.4462824404885204 1106 70-71 0.695190408076293 1106 72-73 0.9198724553765274 1106 74-75 1.0777089612192228 1106 76-77 0.8920011403878298 1106 78-79 0.9125501563793534 1106 80-81 1.0277070543496798 1106 82-83 0.999510872579183 1106 84-85 1.003881624841874 1106 86-87 0.6820602981044495 1106 88-89 0.8361693321954036 1106 90-91 0.9656404417027069 1106 92-93 0.8400535698156588 1106 94-95 1.049329135890705 1106 96-97 1.060172577243364 1106 98-99 1.1106287995408586 1106 100-101 1.2422937441352957 1107 1 -0.029821500177508398 1107 2 -0.14504258971753003 1107 3 -0.21509614644704556 1107 4 0.25888419697464116 1107 5 0.20792295490031876 1107 6 0.002845605570271914 1107 7 -0.20509466738488413 1107 8 0.22704079253911402 1107 9 0.039662179688491506 1107 10-11 0.008067676862026474 1107 12-13 0.2053849271867847 1107 14-15 0.4374257513461046 1107 16-17 0.1385320226424298 1107 18-19 0.11657416245220986 1107 20-21 0.08478061918785329 1107 22-23 0.042463726631147836 1107 24-25 -0.047113059860187434 1107 26-27 -0.17880305266573515 1107 28-29 0.0556035261900476 1107 30-31 0.15675115685869656 1107 32-33 0.12000959779177123 1107 34-35 -0.03874397895862103 1107 36-37 -0.15010443761247672 1107 38-39 -0.07483503998696506 1107 40-41 -0.19428673658039486 1107 42-43 -0.2589862973378487 1107 44-45 -0.24232933010770807 1107 46-47 -0.07648976528120954 1107 48-49 -0.19075086983476552 1107 50-51 -0.08280197830130476 1107 52-53 -0.21769186173085586 1107 54-55 -0.2513085593976143 1107 56-57 -0.28434241299911633 1107 58-59 -0.40590842501737967 1107 60-61 -0.35742878423642566 1107 62-63 -0.40993324583780577 1107 64-65 -0.3529310894071216 1107 66-67 -0.5033294480899357 1107 68-69 -0.33068692157938884 1107 70-71 0.1254231090034139 1107 72-73 0.26854624428792917 1107 74-75 0.16678306940366383 1107 76-77 0.43778612227522373 1107 78-79 0.2117396940474343 1107 80-81 0.04845366207363355 1107 82-83 0.16370931305317882 1107 84-85 0.26320808152512143 1107 86-87 0.534424146572551 1107 88-89 0.6775121209226143 1107 90-91 0.5934755248978938 1107 92-93 0.5704766065934521 1107 94-95 0.2860117662093664 1107 96-97 0.4675711159552165 1107 98-99 0.3644877054167104 1107 100-101 0.5408385958575295 1108 1 0.10982146364446521 1108 2 0.01821536540695945 1108 3 -0.02871517900551268 1108 4 0.01719073502394508 1108 5 0.008495759004830461 1108 6 0.12540987032524953 1108 7 -0.1703925782312652 1108 8 0.07586267857569595 1108 9 0.26098244043735264 1108 10-11 -0.0045788186662321095 1108 12-13 0.09725936712142413 1108 14-15 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-0.020100017265164638 2316 96-97 -0.6052825166409619 2316 98-99 -0.9053164534566527 2316 100-101 -1.1992868304820021 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1635.0 3 308.0 4 236.0 5 261.0 6 352.0 7 411.0 8 472.0 9 662.0 10 924.0 11 1428.0 12 2180.0 13 3181.0 14 3767.0 15 5204.0 16 7494.0 17 10518.0 18 8424.0 19 4440.0 20 4200.0 21 4000.0 22 4230.0 23 4619.0 24 4812.0 25 5155.0 26 5795.0 27 6736.0 28 7583.0 29 8986.0 30 10960.0 31 13284.0 32 16888.0 33 21666.0 34 30182.0 35 45127.0 36 62635.0 37 88508.0 38 79202.0 39 11983.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.79738702956782 18.73025647341359 15.424120200745097 20.04823629627349 2 15.130720295505848 21.28688692797045 41.0336548327519 22.548737943771805 3 18.358962150891585 27.345226637005236 31.850261760764408 22.445549451338767 4 11.639871778559439 17.336778292651893 38.45904424953107 32.56430567925759 5 12.175372644297065 38.096723356753564 34.012000131328385 15.715903867620987 6 28.51827542355499 37.41293022111007 18.17081675349715 15.897977601837798 7 25.798187117829137 35.88206214834127 21.114190243912446 17.20556048991715 8 26.294306711873304 32.5340126636023 20.686345092392607 20.48533553213179 9 27.648830035115356 15.87328935709363 21.136490433526962 35.341390174264056 10-11 24.02321273623513 27.27927284811412 27.142347243333724 21.555167172317027 12-13 26.706345584019576 25.511362645680535 28.22563854304595 19.55665322725394 14-15 23.053580877919117 25.46076191335666 29.36293230399416 22.122724904730067 16-17 19.64267216401799 29.410932139505547 29.41636795331354 21.530027743162925 18-19 19.42861479235268 26.09781784955786 35.030082890746854 19.443484467342603 20-21 20.67503235585492 24.768176196346197 35.25465237197645 19.30213907582243 22-23 20.315651839507755 25.10078195103192 35.125035251890786 19.458530957569543 24-25 20.023503738858285 25.28723373709448 35.33959416057196 19.349668363475274 26-27 19.74754969576949 25.52685258588243 35.309199319442556 19.416398398905528 28-29 19.227459888523683 25.954189079012814 35.61294027761397 19.205410754849527 30-31 19.259686197853902 25.943596017939168 35.36166010134626 19.435057682860666 32-33 19.012699952720034 26.15915400654125 34.974765215482805 19.85338082525591 34-35 19.744050439230694 25.934711223508227 34.62436186948398 19.6968764677771 36-37 19.93293167542623 25.731675426227408 34.585412126650425 19.749980771695935 38-39 19.705142219606188 26.033499387806586 34.65794077413242 19.60341761845481 40-41 19.494989478520182 26.249143719722547 34.652893714595116 19.602973087162155 42-43 19.401205096022117 26.643471234382567 33.80985487985956 20.145468789735766 44-45 19.498207297162033 27.020277643313666 33.325607344748654 20.155907714775644 46-47 19.87300506482546 27.145885226686573 32.31807801205344 20.663031696434523 48-49 20.121524751053727 27.541097927413315 31.66904246700372 20.66833485452923 50-51 20.157484998620625 27.88025971282268 31.363266695860943 20.598988592695754 52-53 20.27950297819274 28.06895377647966 30.92920844067407 20.722334804653528 54-55 19.594922376190098 27.89595732613487 31.273621237652854 21.23549906002217 56-57 20.178851217771506 28.305496993511237 30.551750162014063 20.963901626703198 58-59 20.38381918234641 27.499454015828928 30.907420565711373 21.20930623611329 60-61 20.312416686832726 27.149736063886078 31.82665529164223 20.71119195763897 62-63 20.519645913164027 27.233678649687615 31.644895979557873 20.60177945759048 64-65 20.178413046710382 27.421162453281926 31.676826298493232 20.72359820151446 66-67 20.00496322191037 27.63714745998915 31.41914306487157 20.938746253228917 68-69 20.007916008281047 27.545658038696157 31.542524447853292 20.903901505169504 70-71 19.96115624766071 27.775846528211456 31.10279135634221 21.160205867785628 72-73 20.05384063173008 27.41349605168701 31.093118654496976 21.43954466208594 74-75 19.732110170768998 27.74583485869745 31.061027895448724 21.461027075084832 76-77 19.94864269240275 27.37945441064721 31.169414508234293 21.502488388715747 78-79 19.728599218797267 27.279952172098408 31.386137497013323 21.605311112091005 80-81 19.56601763450695 27.53353765763955 31.18799647678998 21.712448231063515 82-83 19.609035541011583 27.576920749810025 30.96519735378058 21.84884635539781 84-85 19.622027447152337 27.022443337954343 30.71603763524654 22.63949157964678 86-87 19.924202573544115 26.99821888352606 30.72554092312488 22.35203761980495 88-89 19.893846436236593 26.545876653906774 30.750386334574458 22.809890575282175 90-91 19.387790677404734 26.52852376176911 31.520002625043325 22.56368293578283 92-93 19.653383103964558 26.576081383186008 31.46932748118219 22.301208031667247 94-95 19.27534909190472 26.658141683578197 31.092431804408037 22.974077420109047 96-97 19.345180111358346 26.665094387990525 30.964285348071733 23.025440152579392 98-99 19.5591159348508 26.839948029947386 30.50194990242797 23.098986132773845 100-101 19.72239966286847 26.523782230256327 31.01405022316508 22.73976788371013 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1255.0 1 838.0 2 534.0 3 638.0 4 518.0 5 485.0 6 904.0 7 1998.0 8 3327.0 9 3700.5 10 2853.5 11 2136.5 12 2123.0 13 2188.0 14 2103.0 15 2044.0 16 2089.5 17 2088.5 18 2105.5 19 2187.5 20 2296.5 21 2436.5 22 2720.5 23 3071.5 24 3386.5 25 3828.0 26 4379.5 27 4911.0 28 5461.5 29 6057.5 30 6751.0 31 7562.5 32 8470.0 33 9312.5 34 10190.0 35 10830.5 36 11607.5 37 12660.5 38 13243.5 39 13187.0 40 13185.0 41 14036.0 42 14891.5 43 15565.0 44 16209.0 45 16646.5 46 16945.0 47 17881.5 48 21811.5 49 21379.0 50 16777.0 51 14875.0 52 13476.5 53 12866.0 54 12483.0 55 11312.0 56 9951.5 57 8721.5 58 7842.0 59 7079.0 60 6119.5 61 5230.5 62 4192.0 63 3033.5 64 2143.0 65 1612.5 66 1153.0 67 846.0 68 597.0 69 379.0 70 291.0 71 253.0 72 219.5 73 175.0 74 132.5 75 100.0 76 68.5 77 38.5 78 22.0 79 15.5 80 9.5 81 10.0 82 7.5 83 3.0 84 1.5 85 1.0 86 2.0 87 2.0 88 1.0 89 1.0 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2591866942641125 2 0.23564287922432345 3 0.24035164223228123 4 0.23932799810011648 5 0.22991047208420087 6 0.18650796088041585 7 0.19244509684697134 8 0.1867126897068488 9 0.18732687618614766 10-11 0.1965396733756303 12-13 0.1954136648302491 14-15 0.18200392669889098 16-17 0.19316164773948666 18-19 0.18046846050064388 20-21 0.1850748590953852 22-23 0.1819015622856745 24-25 0.17790935017023202 26-27 0.18620086764076643 28-29 0.18497249468216873 30-31 0.18722451177293115 32-33 0.1902954441694254 34-35 0.18302757083105572 36-37 0.1819015622856745 38-39 0.1760667907323355 40-41 0.1795471807816956 42-43 0.17504314660017076 44-45 0.18814579149187943 46-47 0.17862590106274734 48-49 0.1834370284839216 50-51 0.18681505412006527 52-53 0.18568904557468405 54-55 0.19234273243375488 56-57 0.17176748537724357 58-59 0.16368069673314212 60-61 0.1705391124186459 62-63 0.1700272903525635 64-65 0.16757054443536812 66-67 0.17698807045128376 68-69 0.17023201917899647 70-71 0.17535023983982018 72-73 0.18446067261608637 74-75 0.17657861279841786 76-77 0.1814921046328086 78-79 0.1780117145834485 80-81 0.16941310387326466 82-83 0.18169683345924156 84-85 0.1684918241543164 86-87 0.17105093448472825 88-89 0.1751455110133872 90-91 0.17197221420367653 92-93 0.1803660960874274 94-95 0.17698807045128376 96-97 0.17115329889794473 98-99 0.17657861279841786 100-101 0.1653185273446057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 488451.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.14626939906427 #Duplication Level Percentage of deduplicated Percentage of total 1 79.21655510115068 43.68497488473874 2 11.798920439434383 13.013328903423485 3 3.7928495072425155 6.274845021495118 4 1.7143769667423405 3.7816597623809503 5 0.8897867758059737 2.4534210623160515 6 0.5306799332928003 1.7559011139625331 7 0.3472118027833863 1.3403204930379176 8 0.2655670487190294 1.1716025609739218 9 0.2017864692745563 1.0014993896130617 >10 1.0991673802493291 11.520932660300446 >50 0.0877175682902391 3.283827526909334 >100 0.049062368630508724 4.681519295075053 >500 0.00408853071920906 1.5659723036065416 >1k 0.0014867384433487492 1.6514448756751183 >5k 7.433692216743746E-4 2.818750146491775 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7961 1.629846187232701 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5791 1.1855846338732032 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4294 0.8791055807030798 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1486 0.3042270360793611 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1218 0.24935971059533096 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1059 0.21680782719249217 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 921 0.18855524914474533 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 860 0.1760667907323355 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 811 0.16603507823712102 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 768 0.15723173870050425 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 699 0.14310544967663083 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 692 0.1416723478916002 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 644 0.13184536422281867 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 638 0.13061699126422097 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 562 0.11505760045531692 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 527 0.10789209153016371 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 518 0.1060495320922672 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.0709323964942236E-4 0.0 22-23 0.0 0.0 0.0 4.094576528658965E-4 0.0 24-25 0.0 0.0 0.0 0.0013307373718141635 0.0 26-27 0.0 0.0 0.0 0.002661474743628327 0.0 28-29 0.0 0.0 0.0 0.006141864792988448 0.0 30-31 0.0 0.0 0.0 0.01044117014808036 0.0 32-33 0.0 0.0 0.0 0.016583034941068806 0.0 34-35 0.0 0.0 0.0 0.022827264147273728 0.0 36-37 0.0 0.0 0.0 0.027638391568448013 0.0 38-39 0.0 0.0 0.0 0.03500862932003415 0.0 40-41 0.0 0.0 0.0 0.045142706228465085 0.0 42-43 0.0 0.0 0.0 0.06285174971491511 0.0 44-45 0.0 0.0 0.0 0.09223033630804317 0.0 46-47 1.0236441321647412E-4 0.0 0.0 0.124577490884449 0.0 48-49 2.0472882643294825E-4 0.0 0.0 0.20943758944090607 0.0 50-51 2.0472882643294825E-4 0.0 0.0 0.2904078402951371 0.0 52-53 2.0472882643294825E-4 0.0 0.0 0.37301592176083165 0.0 54-55 2.0472882643294825E-4 0.0 0.0 0.5284051010234394 0.0 56-57 2.0472882643294825E-4 0.0 0.0 0.6769358646005433 0.0 58-59 2.0472882643294825E-4 0.0 0.0 0.8793103095295127 0.0 60-61 2.0472882643294825E-4 0.0 0.0 1.1084018663079818 0.0 62-63 2.0472882643294825E-4 0.0 0.0 1.4087390546851168 0.0 64-65 2.0472882643294825E-4 0.0 0.0 1.6228854071339809 0.0 66-67 2.0472882643294825E-4 0.0 0.0 1.9410340034107822 0.0 68-69 2.0472882643294825E-4 0.0 0.0 2.3547909616317706 0.0 70-71 2.0472882643294825E-4 0.0 0.0 2.630970148489818 1.0236441321647412E-4 72-73 2.0472882643294825E-4 0.0 0.0 3.090074541765704 2.0472882643294825E-4 74-75 2.0472882643294825E-4 0.0 0.0 3.6718115020749265 2.0472882643294825E-4 76-77 2.0472882643294825E-4 0.0 0.0 4.1144352248229605 2.0472882643294825E-4 78-79 2.0472882643294825E-4 0.0 0.0 4.513144614301128 2.0472882643294825E-4 80-81 2.0472882643294825E-4 0.0 0.0 5.114637906361129 2.0472882643294825E-4 82-83 2.0472882643294825E-4 0.0 0.0 5.838251943388385 2.0472882643294825E-4 84-85 2.0472882643294825E-4 0.0 0.0 6.564629819572485 2.0472882643294825E-4 86-87 2.0472882643294825E-4 0.0 0.0 7.549477839128183 2.0472882643294825E-4 88-89 2.0472882643294825E-4 0.0 0.0 8.757992101561877 2.0472882643294825E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 25 0.0050105453 57.025852 4 GGTATCA 4210 0.0 34.09608 1 GTATCAA 6335 0.0 32.41282 1 GAATAGT 60 0.003936028 31.687529 1 AGGTAAG 60 0.003941964 31.677776 2 GTACATG 3380 0.0 27.562525 1 TACATGG 3485 0.0 26.451174 2 TATCAAC 7765 0.0 26.313156 2 ATCAACG 7785 0.0 26.24556 3 GAGTACT 4830 0.0 26.1087 12-13 TCAACGC 7885 0.0 25.915361 4 CAACGCA 7950 0.0 25.706112 5 AACGCAG 8055 0.0 25.296452 6 ACATGGG 3520 0.0 25.24323 3 GTACTTT 5215 0.0 24.22675 14-15 AGTACTT 4845 0.0 24.018183 12-13 CATGGGA 2455 0.0 24.002735 4 ACGCAGA 8695 0.0 23.270613 7 CGCAGAG 8690 0.0 23.17469 8 ATGGGAG 745 0.0 22.965786 5 >>END_MODULE