FastQCFastQC Report
Thu 26 May 2016
SRR937417_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937417_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488451
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62691.2834450129081525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52301.0707317622443193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37360.7648668955534946No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19490.3990164827178161No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13030.2667616608421316No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11720.23994218457941535No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10850.22213077667974884No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10660.21824092897752284No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9990.2045240976065153No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.18527958792181815No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5520.11301031219098742No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT5160.1056400744394013No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT1750.035.2937364
TAAGGTG1800.034.313365
AGGTAAG1553.6379788E-1133.7173422
ACATACG600.00394435331.6738684
ATACGCC650.005843102529.2284376
TACGCCT700.00839019427.1406947
ATGGGAG8350.025.6046245
GGTAAGG2059.513315E-1025.49363
GGTATCA54900.025.4585861
GTATCAA74750.025.1214771
TATACTA950.00122392125.0056865
GTACATG44400.024.6265831
GTATAGG1551.397575E-624.536781
AGGTGTG3100.024.5141747
AGTACTT47650.023.07528512-13
AAGGTGT3100.022.9820386
GAGTACT47750.022.7782912-13
TACATGG45900.022.772062
ACATGGG43950.022.5933043
TTAGGCT1302.9336417E-421.9280624
Produced by FastQC (version 0.11.5)