##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937417_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 488451 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11885941476218 33.0 31.0 34.0 31.0 34.0 2 32.31125128211428 33.0 31.0 34.0 31.0 34.0 3 32.3587667954411 34.0 31.0 34.0 30.0 34.0 4 36.01847268200905 37.0 35.0 37.0 35.0 37.0 5 35.924393644398315 37.0 35.0 37.0 35.0 37.0 6 36.03220589168617 37.0 35.0 37.0 35.0 37.0 7 35.98226638905438 37.0 35.0 37.0 35.0 37.0 8 35.97036345508557 37.0 35.0 37.0 35.0 37.0 9 37.63813770470323 39.0 37.0 39.0 35.0 39.0 10-11 37.57452743468639 39.0 37.0 39.0 35.0 39.0 12-13 37.48643159702816 39.0 37.0 39.0 35.0 39.0 14-15 38.58999776845579 40.0 38.0 41.0 34.0 41.0 16-17 38.593055393478565 40.0 38.0 41.0 34.5 41.0 18-19 38.69248297167986 40.0 38.0 41.0 34.5 41.0 20-21 38.632977514633 40.0 38.0 41.0 34.0 41.0 22-23 38.50685329746484 40.0 38.0 41.0 34.0 41.0 24-25 38.3520138151012 40.0 38.0 41.0 34.0 41.0 26-27 38.226059522858996 40.0 38.0 41.0 34.0 41.0 28-29 38.09614884604597 40.0 37.0 41.0 34.0 41.0 30-31 38.025018886234236 40.0 37.0 41.0 34.0 41.0 32-33 37.81312455087614 40.0 36.5 41.0 33.0 41.0 34-35 37.624670642500476 40.0 36.0 41.0 33.0 41.0 36-37 37.390196764875085 40.0 35.5 41.0 33.0 41.0 38-39 37.19467459376683 40.0 35.0 41.0 33.0 41.0 40-41 36.9433259426227 39.5 35.0 41.0 32.0 41.0 42-43 36.62515482617499 39.0 35.0 41.0 31.0 41.0 44-45 36.11739867458558 39.0 35.0 41.0 30.5 41.0 46-47 35.48042280597235 39.0 35.0 41.0 27.5 41.0 48-49 34.95653299921589 39.0 35.0 41.0 24.5 41.0 50-51 34.619546279974855 39.0 35.0 40.5 23.0 41.0 52-53 34.33626402648372 38.5 34.5 40.0 21.5 41.0 54-55 34.07841216416795 38.0 34.0 40.0 20.0 41.0 56-57 33.80521280537864 38.0 34.0 40.0 18.5 41.0 58-59 33.541793342628026 38.0 34.0 40.0 15.0 41.0 60-61 33.181143041983745 37.0 33.0 40.0 10.5 41.0 62-63 32.8136803896399 37.0 33.0 40.0 7.5 41.0 64-65 32.43129095856084 36.0 32.5 39.0 3.5 41.0 66-67 31.987549416420478 35.5 32.0 39.0 2.0 40.5 68-69 31.577024102724735 35.0 31.5 39.0 2.0 40.0 70-71 31.146778284822837 35.0 31.0 38.0 2.0 40.0 72-73 30.70056361845917 35.0 31.0 37.0 2.0 39.5 74-75 30.253740907480996 35.0 30.5 37.0 2.0 39.0 76-77 28.931350329920505 33.5 28.5 35.5 2.0 38.0 78-79 29.5079434784656 34.0 30.0 36.0 2.0 38.0 80-81 29.34381954382323 34.0 30.0 35.5 2.0 37.0 82-83 29.001514993315602 34.0 29.5 35.0 2.0 37.0 84-85 28.636017737705522 34.0 29.0 35.0 2.0 36.5 86-87 28.25042327684865 34.0 29.0 35.0 2.0 36.0 88-89 27.849092334748008 34.0 28.5 35.0 2.0 36.0 90-91 27.392675007319056 34.0 27.0 35.0 2.0 35.0 92-93 27.04035614626646 33.5 26.0 35.0 2.0 35.0 94-95 26.617710886045888 33.0 25.0 35.0 2.0 35.0 96-97 26.135554026913653 33.0 24.5 35.0 2.0 35.0 98-99 25.691662009085867 33.0 23.5 35.0 2.0 35.0 100-101 24.024240916693792 31.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09424109788481161 1101 2 0.06087707872833903 1101 3 0.1683330505014098 1101 4 0.07690408812492677 1101 5 -0.01500325601163155 1101 6 -0.006398394216340364 1101 7 -0.061868951345182666 1101 8 -0.02710835865723027 1101 9 0.24564178413718452 1101 10-11 0.03755317566173488 1101 12-13 0.09263084685328948 1101 14-15 0.07816332161491601 1101 16-17 -0.04059761126861616 1101 18-19 -0.05653467387735844 1101 20-21 0.10583335051173748 1101 22-23 -0.0028142181065433647 1101 24-25 0.0283509151839354 1101 26-27 -0.03146806513483824 1101 28-29 -0.04731515484336768 1101 30-31 0.04763308803353539 1101 32-33 0.10130126020653307 1101 34-35 0.02438936727509855 1101 36-37 0.05959684753116079 1101 38-39 -0.06572291612253167 1101 40-41 -0.010730256630587576 1101 42-43 0.2982578699537939 1101 44-45 0.4244778448516868 1101 46-47 0.5755090111379957 1101 48-49 0.6525331520562361 1101 50-51 0.6221394423927435 1101 52-53 0.545513229301811 1101 54-55 0.8279554666568671 1101 56-57 0.8031395368981435 1101 58-59 0.7655589676774497 1101 60-61 0.7867964600985857 1101 62-63 0.8010762215664826 1101 64-65 0.8485909644130913 1101 66-67 0.7207365731311057 1101 68-69 1.0654054737599203 1101 70-71 0.8226837811315697 1101 72-73 0.9467952981316188 1101 74-75 1.0217666599515063 1101 76-77 1.1684451369784838 1101 78-79 0.8519040935851052 1101 80-81 0.8375346517292321 1101 82-83 0.9283747257432466 1101 84-85 1.0389526522177448 1101 86-87 0.9977903355023052 1101 88-89 1.0474065298302442 1101 90-91 1.0509851867718254 1101 92-93 0.9289905221142085 1101 94-95 0.7880409718979813 1101 96-97 0.6490840946171943 1101 98-99 1.1268518281888085 1101 100-101 1.2246734174795506 1102 1 0.14745426721051302 1102 2 0.20357995698109477 1102 3 0.19555669358182826 1102 4 0.16831804501202896 1102 5 -0.05489283503164444 1102 6 0.01902278874960217 1102 7 0.01829180861096802 1102 8 0.09585676942298704 1102 9 0.03817212960871075 1102 10-11 0.035206729837035766 1102 12-13 0.1629714468614054 1102 14-15 0.12082560391914399 1102 16-17 0.15798153053302855 1102 18-19 0.25679297602317774 1102 20-21 0.07281666557690158 1102 22-23 0.17892166465234993 1102 24-25 0.2030312220893009 1102 26-27 0.24271291160368236 1102 28-29 0.2094663983576197 1102 30-31 0.28428311036130793 1102 32-33 0.3762373215876025 1102 34-35 0.2227086950061974 1102 36-37 0.1846810841243567 1102 38-39 0.08734816738333961 1102 40-41 0.36364558066085095 1102 42-43 0.35329785696308846 1102 44-45 0.4664743536001126 1102 46-47 0.5247356583186118 1102 48-49 0.3233081287135704 1102 50-51 0.5000633655852127 1102 52-53 0.5665500498271214 1102 54-55 0.5827762355441237 1102 56-57 0.6349424029721149 1102 58-59 0.6584627958753941 1102 60-61 0.5135329517967193 1102 62-63 0.5002561834063002 1102 64-65 0.4622271435224192 1102 66-67 0.6332523040611591 1102 68-69 0.6923530643903781 1102 70-71 0.6687764210861005 1102 72-73 0.8641013509875464 1102 74-75 0.885035445726686 1102 76-77 0.9536073177928373 1102 78-79 0.9890412672951676 1102 80-81 1.169727238909033 1102 82-83 0.9805405195970209 1102 84-85 0.9124800341850232 1102 86-87 0.6624109663633462 1102 88-89 0.6040054012628744 1102 90-91 0.6883781149575903 1102 92-93 0.6026478111167677 1102 94-95 0.27544404907088094 1102 96-97 0.4290459344255808 1102 98-99 0.6983737702989856 1102 100-101 1.0123687854818222 1103 1 0.061205901197439516 1103 2 0.07301786547782285 1103 3 0.15134754325503508 1103 4 0.015612162659088824 1103 5 0.0042182760794631236 1103 6 0.022421481560058965 1103 7 -0.133943485506677 1103 8 -0.10314976652472296 1103 9 -0.13016773967233775 1103 10-11 -0.08190438127407873 1103 12-13 0.03286687169800473 1103 14-15 -0.015919494120069544 1103 16-17 -0.09975703155846816 1103 18-19 -0.0719259782251811 1103 20-21 -0.12255588344270762 1103 22-23 -0.005679642537195662 1103 24-25 -0.08524328771461143 1103 26-27 -0.009012578592397347 1103 28-29 -0.09909569314564237 1103 30-31 -0.09663192231842999 1103 32-33 -0.060350913706514575 1103 34-35 -0.056467775582042634 1103 36-37 0.14936082268643958 1103 38-39 0.2804592784944475 1103 40-41 0.157998521837321 1103 42-43 0.4779514517343344 1103 44-45 0.37446128170468285 1103 46-47 0.3917160504754733 1103 48-49 0.4426283901514765 1103 50-51 0.31067774467017983 1103 52-53 0.3668507034218962 1103 54-55 0.28350826168792054 1103 56-57 0.19524305656688057 1103 58-59 0.2212732533917361 1103 60-61 0.1277943897052154 1103 62-63 0.16842611804681695 1103 64-65 0.2312990389472489 1103 66-67 0.021539885760510913 1103 68-69 0.025699469619137716 1103 70-71 0.006162042001133017 1103 72-73 0.30731703726205595 1103 74-75 -0.009082201332137885 1103 76-77 -0.05728157109605192 1103 78-79 0.06965564638013788 1103 80-81 -0.004481911417766327 1103 82-83 0.04170391828983355 1103 84-85 0.17807480542685283 1103 86-87 0.21958743695158134 1103 88-89 0.07261978034784278 1103 90-91 0.1600121018856946 1103 92-93 0.15362820327363025 1103 94-95 0.11879045429963853 1103 96-97 0.21831390828178954 1103 98-99 0.19199468533166453 1103 100-101 0.22583283776940632 1104 1 0.118340113008081 1104 2 0.07136242379895918 1104 3 0.05481850469963945 1104 4 -0.011029125774292936 1104 5 0.05846327363924786 1104 6 0.03952250645024691 1104 7 -0.12367701405718634 1104 8 0.005187959195140479 1104 9 0.009289556569733293 1104 10-11 0.0035490106244040476 1104 12-13 0.04291958033636689 1104 14-15 0.09019373184381863 1104 16-17 -0.05914112135837257 1104 18-19 -0.0018908391329475194 1104 20-21 -0.164021964457838 1104 22-23 -0.26187681188809364 1104 24-25 -0.10466837312213073 1104 26-27 -0.03050501392670668 1104 28-29 -0.04158959260880124 1104 30-31 0.14018603766205473 1104 32-33 0.046791106791289394 1104 34-35 -0.004997790223328025 1104 36-37 -0.15643127101780152 1104 38-39 -0.22750460632741465 1104 40-41 -0.06415277147648624 1104 42-43 0.07654784021652716 1104 44-45 -0.09768415509375217 1104 46-47 0.139796089518903 1104 48-49 0.40727065467075363 1104 50-51 0.35733855482146737 1104 52-53 0.3521518259206715 1104 54-55 0.38726814455760916 1104 56-57 0.2181527686215432 1104 58-59 0.224040456905648 1104 60-61 0.07908477525914748 1104 62-63 0.03992929032597914 1104 64-65 -0.036267578915122556 1104 66-67 0.2923724870294251 1104 68-69 0.47598448670063576 1104 70-71 0.5474211928064072 1104 72-73 0.614151590702761 1104 74-75 0.7788631378577122 1104 76-77 0.732068355697507 1104 78-79 0.7087859538471939 1104 80-81 0.7248894892654967 1104 82-83 0.7289103604567408 1104 84-85 0.9295203886381742 1104 86-87 0.9603468317783239 1104 88-89 0.6305826890838091 1104 90-91 0.5288359183815459 1104 92-93 0.45338418179974127 1104 94-95 0.6937265206734757 1104 96-97 0.6882436300973076 1104 98-99 0.7585188434575798 1104 100-101 1.2266722716395861 1105 1 0.09063189862343535 1105 2 0.14867294913290863 1105 3 0.23831794222542868 1105 4 0.15226081130773395 1105 5 0.08620540606659688 1105 6 0.10404310318168086 1105 7 0.1902881747249836 1105 8 -0.019453498828454485 1105 9 0.1471861856815906 1105 10-11 0.13213937676967902 1105 12-13 0.15359274042387483 1105 14-15 0.26837651617594105 1105 16-17 0.24748363768220116 1105 18-19 0.1783906240914206 1105 20-21 0.05334887846205305 1105 22-23 0.24161250675495438 1105 24-25 0.3219047174333909 1105 26-27 0.28162577404595623 1105 28-29 -0.03398629803625397 1105 30-31 -0.032256117942623064 1105 32-33 0.21240584305024157 1105 34-35 0.012806355692084992 1105 36-37 0.010217965543581897 1105 38-39 -0.09581356577839983 1105 40-41 -0.17066814482934234 1105 42-43 0.0683015160843965 1105 44-45 0.052520483763885295 1105 46-47 -0.35813208337266644 1105 48-49 -0.5140691645460791 1105 50-51 -0.3926247006322683 1105 52-53 -0.45749163892045175 1105 54-55 -0.37859984642019384 1105 56-57 -0.2833721300483063 1105 58-59 -0.4641707620522837 1105 60-61 -0.29451963860881847 1105 62-63 -0.10399344437274038 1105 64-65 -0.15481679116857805 1105 66-67 -0.13305728283665985 1105 68-69 -0.24137646345679897 1105 70-71 -0.03480320896411726 1105 72-73 -0.24919261524759406 1105 74-75 -0.18057512282506138 1105 76-77 -0.5524630382775193 1105 78-79 -0.2925142377897494 1105 80-81 -0.34677792171377675 1105 82-83 -0.15221113962522992 1105 84-85 -0.2568338174817697 1105 86-87 -0.19821436485022303 1105 88-89 -0.12819360446554384 1105 90-91 -0.26719510532151247 1105 92-93 -0.21368196403653883 1105 94-95 -0.273214693705512 1105 96-97 -0.4734775975097172 1105 98-99 -0.2640748127378316 1105 100-101 0.02578135771792489 1106 1 0.038898785092570165 1106 2 0.12943734113325434 1106 3 0.13784941591421074 1106 4 0.1384436233332451 1106 5 0.03299730229669251 1106 6 0.05882597594208505 1106 7 0.13342730101017963 1106 8 0.1338240162468125 1106 9 0.07164499441005745 1106 10-11 -0.16504295805310676 1106 12-13 -0.0010881844817660635 1106 14-15 0.061825824231988236 1106 16-17 0.17674484110070665 1106 18-19 0.20247851615683743 1106 20-21 0.15567632629511508 1106 22-23 0.2853915185114957 1106 24-25 0.4377304950569183 1106 26-27 0.15108479968475308 1106 28-29 0.09974325816521912 1106 30-31 0.15288118629580794 1106 32-33 0.005649086293480821 1106 34-35 -0.08925429243597449 1106 36-37 0.03758554178757123 1106 38-39 0.181230814074965 1106 40-41 -0.19145466168327374 1106 42-43 -0.28387626362147245 1106 44-45 -0.26171100293951355 1106 46-47 -0.4021865712473769 1106 48-49 -0.39176861359383963 1106 50-51 -0.6452623302361928 1106 52-53 -0.5436137160537626 1106 54-55 -0.7238625248289381 1106 56-57 -0.5688543217102691 1106 58-59 -0.548726746608267 1106 60-61 -0.7040333256505917 1106 62-63 -0.5700383014288377 1106 64-65 -0.3864987138617373 1106 66-67 -0.6822503764117727 1106 68-69 -0.40305708468797974 1106 70-71 -0.36080425013369677 1106 72-73 -0.3135368953222155 1106 74-75 -0.3375466208077924 1106 76-77 -0.2892141553657126 1106 78-79 -0.44092862328278315 1106 80-81 -0.37337329718555523 1106 82-83 -0.5465432727214043 1106 84-85 -0.24928099981659102 1106 86-87 -0.2597084431982353 1106 88-89 -0.06341767283567634 1106 90-91 -0.31766580073603023 1106 92-93 0.13040722949085648 1106 94-95 -0.5124754633033568 1106 96-97 -0.19240519284180735 1106 98-99 0.17388232578110774 1106 100-101 0.44890399881809273 1107 1 0.03309661157994981 1107 2 -0.1071132820631604 1107 3 0.10983934653371819 1107 4 0.0773826544623688 1107 5 -0.03285276217189903 1107 6 0.02067284768028088 1107 7 -0.289615616654217 1107 8 -0.11775085942090158 1107 9 0.04696887234952385 1107 10-11 0.12272949850733994 1107 12-13 -0.07715498622548722 1107 14-15 -0.04929654330040023 1107 16-17 -0.1649264304655773 1107 18-19 -0.0796636831432167 1107 20-21 -0.29735369765035813 1107 22-23 -0.15904576275577398 1107 24-25 -0.20236897077472094 1107 26-27 0.008222394085201756 1107 28-29 0.05997534510571967 1107 30-31 0.1141877498127215 1107 32-33 0.05947422290550719 1107 34-35 0.08781637742342241 1107 36-37 0.08184169700338373 1107 38-39 0.35911501619936814 1107 40-41 0.2610258442416935 1107 42-43 0.3277219435376182 1107 44-45 0.6195869647647925 1107 46-47 0.0393772526612608 1107 48-49 0.18953549397661362 1107 50-51 0.14830615997072272 1107 52-53 0.07883977446014256 1107 54-55 -0.07240430661808972 1107 56-57 -0.08036350081016508 1107 58-59 0.2124754391840824 1107 60-61 0.23516050992379434 1107 62-63 -0.07845912785482057 1107 64-65 0.3774848312969752 1107 66-67 0.18854535024138386 1107 68-69 0.32284701060274656 1107 70-71 0.0836483807989481 1107 72-73 0.2940274197757162 1107 74-75 0.23135495713780685 1107 76-77 0.22482771852143557 1107 78-79 0.3443878868172945 1107 80-81 0.5583268317516357 1107 82-83 0.6488733171969336 1107 84-85 0.5860201606181121 1107 86-87 0.6213469997931114 1107 88-89 0.6254722393642353 1107 90-91 0.545203358716293 1107 92-93 0.6093659081916627 1107 94-95 0.5264297985619315 1107 96-97 0.6183794820522799 1107 98-99 0.623338947626749 1107 100-101 1.0727727284797872 1108 1 0.05515659787482008 1108 2 0.13843694372005189 1108 3 0.07300241999672608 1108 4 0.06952856137291263 1108 5 -0.023417308057723574 1108 6 -0.14941131586759582 1108 7 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-0.7948672997719051 2316 98-99 -1.0025824958763891 2316 100-101 -1.8325340526109066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 9.0 5 19.0 6 43.0 7 76.0 8 176.0 9 327.0 10 595.0 11 1061.0 12 1725.0 13 2541.0 14 3192.0 15 4606.0 16 6704.0 17 9811.0 18 8333.0 19 4359.0 20 3478.0 21 3542.0 22 3587.0 23 3588.0 24 3893.0 25 4423.0 26 5141.0 27 6039.0 28 7236.0 29 8861.0 30 10877.0 31 13043.0 32 16649.0 33 21700.0 34 30153.0 35 45007.0 36 65338.0 37 97902.0 38 85628.0 39 8787.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.19628067429679 19.744834099145145 15.421394521799517 19.637490704758548 2 15.707819208067953 22.634000135121028 39.18284536217553 22.47533529463549 3 19.364685505813277 27.584343158269714 31.118576888981696 21.932394446935312 4 12.340848928551686 18.222912840796724 37.1445651662091 32.29167306444249 5 12.438033028574939 39.53907425798327 32.71507990949392 15.307812803947868 6 29.090942591989783 37.78987042712575 17.340326870044283 15.778860110840187 7 26.57441585747598 36.41982512063646 20.01347115677929 16.99228786510827 8 27.31471529385752 33.4549422562345 19.48527078458228 19.745071665325693 9 28.190954670990536 16.36745548683491 20.254232256664434 35.187357585510114 10-11 24.706572409514976 28.102204724731855 26.165265297849732 21.025957567903433 12-13 27.113380005589207 26.291480146547748 27.307671440694538 19.287468407168515 14-15 23.276234463641185 26.47420109693705 28.157686236695184 22.091878202726576 16-17 20.08768535636123 30.250321936079565 28.308161924123404 21.3538307834358 18-19 19.973241942385215 27.400496672132928 33.301088543170145 19.325172842311716 20-21 21.47288059600656 25.840053557060994 33.520148387453396 19.166917459479045 22-23 20.806394156160557 26.534765357890127 33.19384333170913 19.464997154240184 24-25 20.80280314709152 26.67637081303959 33.38072805665256 19.140097983216332 26-27 20.566368202798472 26.774020847771148 33.57887420397977 19.08073674545062 28-29 19.83518705245863 27.271117298705555 33.65654414888444 19.237151499951374 30-31 19.88827947941554 27.190035438559857 33.5338652188244 19.3878198632002 32-33 19.808230508280257 27.463553150674276 33.142526067097826 19.58569027394764 34-35 20.429889589743905 27.155845724545554 32.995837862958616 19.418426822751925 36-37 20.687540817809772 26.965550280376128 32.80329040169843 19.543618500115674 38-39 20.397849528407146 27.17068856446194 33.03350796702228 19.39795394010863 40-41 19.990950985871663 27.618123414631153 32.87248874503277 19.51843685446442 42-43 20.109974262214187 28.02367794392562 32.012113344540296 19.854234449319904 44-45 20.197931829395372 28.219821435517584 31.49834886201482 20.083897873072225 46-47 20.57698725153598 28.57819924618846 30.527422402656562 20.317391099619 48-49 21.003846854648675 28.879969536350625 29.781083465895247 20.33510014310545 50-51 21.05339123064545 29.223402142691896 29.442769080214802 20.280437546447853 52-53 21.03598928039865 29.706050351007573 28.957868854808368 20.300091513785414 54-55 20.132520969350047 29.455769360693296 29.23875680467437 21.17295286528229 56-57 20.82634696213131 29.964520494379173 28.736864086673997 20.472268456815527 58-59 20.944066037330252 29.124518119524783 28.918458555720022 21.012957287424943 60-61 20.696344157346385 28.934939226247874 29.850281809229585 20.518434807176156 62-63 20.911514153927417 29.093399337906977 29.80595801830685 20.189128489858756 64-65 20.57668015829633 29.130557619904557 29.7534450743268 20.539317147472318 66-67 20.257098460234495 29.389232492102586 29.340814124651192 21.012854923011727 68-69 20.43541726805759 29.4439974531734 29.25411146665684 20.866473812112165 70-71 20.401432282869724 29.725601953931918 29.07671393855269 20.796251824645665 72-73 20.31675773724585 29.364682904458807 28.835866180501956 21.482693177793383 74-75 20.238058679376234 29.77002810926787 28.544521354240242 21.447391857115658 76-77 20.27726424963814 29.229851100724535 29.21900047292359 21.273884176713732 78-79 20.001699249259396 29.222890320625815 29.05327248792612 21.722137942188674 80-81 19.89319297124993 29.64258441481336 28.900954241060006 21.563268372876706 82-83 19.86283140546627 29.749820861910127 28.62288872965503 21.764459002968575 84-85 19.716816188483477 29.495072126703874 28.498646728604594 22.289464956208054 86-87 19.895649717167128 29.093092244667325 28.24551490323492 22.765743134930627 88-89 19.908957090885266 28.795621259860255 28.47174025644333 22.823681392811153 90-91 19.602463514111154 28.818426397602003 28.799384940459618 22.779725147827225 92-93 19.52451389350954 28.81138709892056 28.7144473049816 22.9496517025883 94-95 19.47513670767385 28.94435675226379 28.357399206880523 23.223107333181833 96-97 19.75622938636629 28.89235563034982 28.082243664154642 23.26917131912925 98-99 19.49278329409356 29.107073395434536 27.586242194697512 23.81390111577439 100-101 19.12792078559202 29.22966199559374 27.660238822594778 23.98217839621946 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1206.0 1 819.0 2 483.0 3 561.0 4 530.5 5 592.0 6 996.5 7 1775.0 8 2463.0 9 2684.5 10 2378.0 11 2061.5 12 2096.0 13 2094.0 14 2145.5 15 2166.0 16 2085.5 17 2041.5 18 2086.0 19 2147.5 20 2212.5 21 2415.0 22 2663.0 23 2919.0 24 3159.5 25 3584.0 26 4148.5 27 4816.5 28 5425.0 29 5921.0 30 6597.0 31 7320.0 32 8202.5 33 8992.5 34 10122.5 35 10969.5 36 11717.5 37 12849.0 38 13225.0 39 12934.5 40 12870.5 41 13737.5 42 14654.5 43 15578.5 44 16520.5 45 16791.0 46 16572.0 47 17585.5 48 22018.5 49 21375.0 50 16934.5 51 15303.0 52 13722.5 53 13073.5 54 12543.5 55 11485.0 56 10250.5 57 9142.0 58 8562.5 59 7837.5 60 6907.0 61 5879.5 62 4622.5 63 3306.0 64 2132.0 65 1583.5 66 1176.0 67 892.5 68 611.5 69 375.0 70 304.0 71 250.5 72 220.0 73 183.5 74 139.0 75 111.0 76 82.5 77 50.5 78 28.0 79 12.5 80 7.0 81 5.5 82 5.5 83 4.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06100919027701857 2 0.0 3 0.0 4 0.0 5 0.017606679073233548 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0019449238511130083 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010236441321647412 24-25 0.0 26-27 0.0011260085453812154 28-29 0.0037874832890095423 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.013102644891708686 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.0945765286589644E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.0472882643294822E-4 84-85 0.0010236441321647412 86-87 0.0 88-89 0.0 90-91 0.008803339536616774 92-93 7.165508925153187E-4 94-95 0.0 96-97 0.0 98-99 2.0472882643294822E-4 100-101 0.011260085453812154 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 488451.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.26748151551301 #Duplication Level Percentage of deduplicated Percentage of total 1 77.89144368867387 41.490810369030584 2 12.247125413645797 13.047470531790944 3 4.087717622181394 6.532272686405527 4 1.8438149959547587 3.9286152486018326 5 1.0213199169670928 2.7201569899234954 6 0.6007912321059413 1.9201581510531311 7 0.42160329848512906 1.572042213625515 8 0.28963033108995206 1.234230264613275 9 0.22618544969385998 1.0843496334581118 >10 1.2090992364039104 12.161561901712213 >50 0.09722426157824393 3.599124119628927 >100 0.05941482648125641 5.616220173137516 >500 0.0015432422462664003 0.6107803847542702 >1k 0.0023148633693996007 2.1190297938093137 >5k 7.716211231332001E-4 2.3631775384553677 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6269 1.2834450129081525 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5230 1.0707317622443193 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3736 0.7648668955534946 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1949 0.3990164827178161 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1303 0.2667616608421316 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1172 0.23994218457941535 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1085 0.22213077667974884 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1066 0.21824092897752284 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 999 0.2045240976065153 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 905 0.18527958792181815 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 552 0.11301031219098742 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 516 0.1056400744394013 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.0709323964942236E-4 0.0 22-23 0.0 0.0 0.0 4.094576528658965E-4 0.0 24-25 0.0 0.0 0.0 0.0013307373718141635 0.0 26-27 0.0 0.0 0.0 0.002661474743628327 0.0 28-29 0.0 0.0 0.0 0.006141864792988448 0.0 30-31 0.0 0.0 0.0 0.01044117014808036 0.0 32-33 0.0 0.0 0.0 0.016787763767501754 0.0 34-35 0.0 0.0 0.0 0.022827264147273728 0.0 36-37 0.0 0.0 0.0 0.02733129832879859 0.0 38-39 0.0 0.0 0.0 0.034906264906817676 0.0 40-41 0.0 0.0 0.0 0.04555216388133099 0.0 42-43 0.0 0.0 0.0 0.06336357178099748 0.0 44-45 0.0 0.0 0.0 0.09243506513447614 0.0 46-47 0.0 0.0 0.0 0.12590822825626316 0.0 48-49 0.0 0.0 0.0 0.21138251329201907 0.0 50-51 0.0 0.0 0.0 0.29317167945198186 0.0 52-53 0.0 0.0 0.0 0.3768034050498412 0.0 54-55 0.0 0.0 0.0 0.5338304149239126 0.0 56-57 0.0 0.0 0.0 0.6833848226331812 0.0 58-59 0.0 0.0 0.0 0.8873970981736141 0.0 60-61 0.0 0.0 0.0 1.119252494108928 0.0 62-63 0.0 0.0 0.0 1.421739335163609 0.0 64-65 0.0 0.0 0.0 1.6367046029182046 0.0 66-67 0.0 0.0 0.0 1.9592548689633147 0.0 68-69 0.0 0.0 0.0 2.3774134969526113 0.0 70-71 0.0 0.0 0.0 2.655844700901421 0.0 72-73 0.0 0.0 0.0 3.1188389418795337 0.0 74-75 0.0 0.0 0.0 3.704568114304198 0.0 76-77 0.0 0.0 0.0 4.153538430671654 0.0 78-79 0.0 0.0 0.0 4.5594133290749745 0.0 80-81 0.0 0.0 0.0 5.170528875977324 0.0 82-83 0.0 0.0 0.0 5.9040722610865775 0.0 84-85 0.0 0.0 0.0 6.639151112394078 0.0 86-87 0.0 0.0 0.0 7.637101776841485 0.0 88-89 0.0 0.0 0.0 8.86527000661274 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 175 0.0 35.293736 4 TAAGGTG 180 0.0 34.31336 5 AGGTAAG 155 3.6379788E-11 33.717342 2 ACATACG 60 0.003944353 31.673868 4 ATACGCC 65 0.0058431025 29.228437 6 TACGCCT 70 0.008390194 27.140694 7 ATGGGAG 835 0.0 25.604624 5 GGTAAGG 205 9.513315E-10 25.4936 3 GGTATCA 5490 0.0 25.458586 1 GTATCAA 7475 0.0 25.121477 1 TATACTA 95 0.001223921 25.005686 5 GTACATG 4440 0.0 24.626583 1 GTATAGG 155 1.397575E-6 24.53678 1 AGGTGTG 310 0.0 24.514174 7 AGTACTT 4765 0.0 23.075285 12-13 AAGGTGT 310 0.0 22.982038 6 GAGTACT 4775 0.0 22.77829 12-13 TACATGG 4590 0.0 22.77206 2 ACATGGG 4395 0.0 22.593304 3 TTAGGCT 130 2.9336417E-4 21.928062 4 >>END_MODULE