FastQCFastQC Report
Thu 26 May 2016
SRR937416_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937416_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625891
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87991.4058358404258888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65601.0481058203425198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46740.7467753969940453No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17530.2800807169299447No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13970.2232018035089177No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12570.20083369148941269No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.1906082688519247No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA10160.16232858437012196No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9450.1509847561316587No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT9040.14443409475451796No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8880.14187773909514595No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8360.13356958320218695No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8190.13085345531410422No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6400.10225422637487996No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG6380.10193468191745847No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA500.0016147938.002219
GACGCGC250.001617315737.99308460-61
AAGACCG550.002572934834.56135
GTATCAA81350.029.9068281
GGTATCA55850.028.7575241
TGCGATT350.00839765727.13900656-57
GTACATG46000.025.101891
ATAGTAC1351.3391835E-524.6350083
TATCAAC98950.024.3433632
TACATGG47100.024.1082362
AGTACTT57600.023.99879312-13
ATCAACG100250.023.9802973
GAGTACT56950.023.93905612-13
TCAACGC100450.023.9363844
CAACGCA101150.023.772645
GTATAGG1601.8511855E-623.7589911
AACGCAG102350.023.4399786
CTGGACG2503.8016879E-1022.808634
CCTATAC1252.250972E-422.8049763
ACATGGG47100.022.6960373
Produced by FastQC (version 0.11.5)