##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937416_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 625891 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89786720051894 33.0 31.0 34.0 30.0 34.0 2 31.949599850453193 33.0 31.0 34.0 30.0 34.0 3 31.96215794762986 33.0 31.0 34.0 30.0 34.0 4 35.60706257159793 37.0 35.0 37.0 33.0 37.0 5 35.645580460495516 37.0 35.0 37.0 33.0 37.0 6 35.70295786327012 37.0 35.0 37.0 35.0 37.0 7 35.59196729142934 37.0 35.0 37.0 33.0 37.0 8 35.61190047468329 37.0 35.0 37.0 33.0 37.0 9 37.25352177935136 39.0 37.0 39.0 34.0 39.0 10-11 37.147754960528275 39.0 37.0 39.0 34.0 39.0 12-13 37.00014219728355 39.0 37.0 39.0 33.0 39.0 14-15 38.098402117940665 40.0 38.0 41.0 33.0 41.0 16-17 38.071051508968814 40.0 38.0 41.0 33.0 41.0 18-19 38.13804001016152 40.0 38.0 41.0 33.5 41.0 20-21 38.096980145105135 40.0 38.0 41.0 33.5 41.0 22-23 37.94938974997244 40.0 38.0 41.0 33.0 41.0 24-25 37.85745521184999 40.0 37.5 41.0 33.0 41.0 26-27 37.824550920208154 40.0 37.0 41.0 33.0 41.0 28-29 37.717442014663895 40.0 37.0 41.0 33.0 41.0 30-31 37.39952004422496 39.0 36.5 41.0 33.0 41.0 32-33 37.04743317925964 39.0 35.5 41.0 32.0 41.0 34-35 36.99880250714581 39.0 35.0 41.0 32.0 41.0 36-37 36.806709155428024 39.0 35.0 41.0 31.0 41.0 38-39 36.635951787132264 39.0 35.0 41.0 31.0 41.0 40-41 36.35136788993611 39.0 35.0 41.0 30.5 41.0 42-43 35.962729932208646 39.0 35.0 41.0 30.0 41.0 44-45 35.53949409721501 39.0 35.0 41.0 28.0 41.0 46-47 34.920124270839494 39.0 35.0 40.5 24.0 41.0 48-49 34.38529312611941 38.5 35.0 40.0 21.0 41.0 50-51 33.29998194573815 37.5 33.0 39.5 16.5 40.5 52-53 33.374126645054815 38.0 33.5 39.5 15.0 40.5 54-55 33.716655136437495 38.0 34.0 40.0 12.5 41.0 56-57 33.49385835552836 38.0 34.0 40.0 8.5 41.0 58-59 33.21880487177479 38.0 33.0 40.0 7.0 41.0 60-61 32.90240153636975 37.5 33.0 40.0 2.0 41.0 62-63 32.577328160973714 37.0 33.0 40.0 2.0 41.0 64-65 32.1447272767942 36.0 32.0 39.5 2.0 41.0 66-67 31.77982987453087 36.0 32.0 39.0 2.0 41.0 68-69 31.306341679301987 35.0 31.0 39.0 2.0 40.0 70-71 30.826767759881513 35.0 31.0 38.5 2.0 40.0 72-73 30.377830165316325 35.0 30.5 37.5 2.0 40.0 74-75 29.91989739427472 35.0 30.0 37.0 2.0 39.0 76-77 29.45171203931675 34.5 29.0 36.5 2.0 39.0 78-79 28.964772620152708 34.0 29.0 36.0 2.0 38.5 80-81 28.489247329007767 34.0 28.5 35.5 2.0 37.0 82-83 28.02922713379806 34.0 27.5 35.0 2.0 37.0 84-85 27.53537357143656 34.0 26.0 35.0 2.0 36.0 86-87 27.111583326809303 34.0 26.0 35.0 2.0 36.0 88-89 26.660198820561405 33.0 25.0 35.0 2.0 35.5 90-91 26.16670234913108 33.0 24.0 35.0 2.0 35.0 92-93 25.714242575783963 33.0 22.0 35.0 2.0 35.0 94-95 25.2333537309212 32.5 19.0 35.0 2.0 35.0 96-97 24.558730673551786 32.0 8.5 34.5 2.0 35.0 98-99 23.73187663666677 32.0 2.0 34.0 2.0 35.0 100-101 21.40506094511664 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.04561098602960101 1101 2 -0.1399694535666569 1101 3 -0.07777053745848761 1101 4 -0.004344743319862232 1101 5 0.007696312205226263 1101 6 -0.13371363526358238 1101 7 -0.21051922120841482 1101 8 -0.38184062254195084 1101 9 -0.33426168021562574 1101 10-11 -0.29871426570912973 1101 12-13 -0.19119148248726958 1101 14-15 -0.3815079338313083 1101 16-17 -0.37165803648306195 1101 18-19 -0.15869742922708951 1101 20-21 -0.09449772029949344 1101 22-23 -0.08118661365384128 1101 24-25 -0.20798967805388457 1101 26-27 -0.22563760702664126 1101 28-29 -0.2926703493559444 1101 30-31 -0.034857095600060006 1101 32-33 -0.2832165784388607 1101 34-35 -0.32295478172932235 1101 36-37 -0.22218449661433937 1101 38-39 -0.14678797277743882 1101 40-41 -0.22742015346607047 1101 42-43 0.04041098019278877 1101 44-45 0.3180570562045233 1101 46-47 0.5761285898249398 1101 48-49 0.461876957388327 1101 50-51 0.6705610949161738 1101 52-53 0.5102122749900957 1101 54-55 0.6055251011677285 1101 56-57 0.5399476280820608 1101 58-59 0.37028173124305397 1101 60-61 0.5544855479044415 1101 62-63 0.4821507712577997 1101 64-65 0.4512193867191634 1101 66-67 0.35308343088537697 1101 68-69 0.46680305029522984 1101 70-71 0.7812643210149481 1101 72-73 0.5597094667217171 1101 74-75 0.9024657402819507 1101 76-77 0.798548709742466 1101 78-79 0.5479664182577615 1101 80-81 0.7621869165827384 1101 82-83 0.3953808470858533 1101 84-85 0.22407011151508982 1101 86-87 0.24735155115009988 1101 88-89 0.2497263914090233 1101 90-91 0.4021103841234037 1101 92-93 0.42377824195698466 1101 94-95 0.4400128515264221 1101 96-97 0.541644191040767 1101 98-99 0.46355261236989875 1101 100-101 0.27084357395277436 1102 1 -0.22595119934186414 1102 2 -0.3059489453040989 1102 3 -0.2247889389332407 1102 4 -0.43917422518173765 1102 5 -0.31790527446933936 1102 6 -0.3904495954979623 1102 7 -0.34636588655833833 1102 8 -0.22411545959508317 1102 9 -0.3098607979982333 1102 10-11 -0.38822394376562386 1102 12-13 -0.23668698910389452 1102 14-15 -0.1251279055213459 1102 16-17 -0.30232743539016127 1102 18-19 -0.3095467280617683 1102 20-21 -0.36522201090453876 1102 22-23 -0.4865848459271973 1102 24-25 -0.4455876107683494 1102 26-27 -0.6068448128993111 1102 28-29 -0.4934015528584794 1102 30-31 -0.5219982833016843 1102 32-33 -0.4270459944636187 1102 34-35 -0.1943193383163333 1102 36-37 -0.4488555249901367 1102 38-39 -0.14196951863299745 1102 40-41 -0.1382190869047406 1102 42-43 -0.2931548404668973 1102 44-45 0.23341109531176585 1102 46-47 0.3778621634637602 1102 48-49 0.43012083453626104 1102 50-51 0.15713443746670208 1102 52-53 0.3179576005546494 1102 54-55 0.5710593693881449 1102 56-57 0.5691031847348995 1102 58-59 0.46929664335049637 1102 60-61 0.5916053385424007 1102 62-63 0.5805896085742788 1102 64-65 0.5885021242265687 1102 66-67 0.4274197104297883 1102 68-69 0.4093971973753554 1102 70-71 0.3640092350975088 1102 72-73 0.39907924090040225 1102 74-75 0.21292067477401488 1102 76-77 0.42023833710436875 1102 78-79 0.43000481775739985 1102 80-81 0.4193174880489323 1102 82-83 0.5626894256624553 1102 84-85 0.42009396101143537 1102 86-87 0.4091935914424134 1102 88-89 0.5316244755488633 1102 90-91 0.32244335535258273 1102 92-93 0.4928352075241733 1102 94-95 0.3690795280686565 1102 96-97 0.2700290419196918 1102 98-99 0.2783054762845758 1102 100-101 0.4855692800558771 1103 1 -0.06030752129139927 1103 2 -0.09022887342663921 1103 3 -0.2264261211060905 1103 4 -0.08056286316148231 1103 5 -0.1277410998109545 1103 6 -0.10359197629475858 1103 7 -0.06144814205004678 1103 8 -0.05204076661178192 1103 9 -0.031179480372877322 1103 10-11 0.09640960609696236 1103 12-13 -0.012218262526538126 1103 14-15 -0.06999282297757503 1103 16-17 -0.04130438271204895 1103 18-19 -0.19687063788822456 1103 20-21 -0.19704448414267972 1103 22-23 -0.06419484823342714 1103 24-25 -0.023028849981919564 1103 26-27 -0.12594987603232255 1103 28-29 -0.3401880873654264 1103 30-31 -0.3906789763234215 1103 32-33 -0.262465343722738 1103 34-35 -0.07014753868917012 1103 36-37 -0.10219751468898153 1103 38-39 -0.27439629587051684 1103 40-41 -0.20229621103750617 1103 42-43 0.03126007410654097 1103 44-45 0.0036158211588315226 1103 46-47 0.06823594068206518 1103 48-49 -0.05363526736061175 1103 50-51 -0.1678288070127678 1103 52-53 -0.09955328196221558 1103 54-55 0.0974176420228261 1103 56-57 -0.11153638534563015 1103 58-59 -0.04277141922516847 1103 60-61 -0.05344323640042603 1103 62-63 0.009285099897098803 1103 64-65 0.12951816212559564 1103 66-67 0.16691495863765482 1103 68-69 0.08931066575119218 1103 70-71 0.28364076700994545 1103 72-73 0.38943752314436253 1103 74-75 0.21700702382173276 1103 76-77 0.21586863422350788 1103 78-79 -0.1672249450854153 1103 80-81 -0.11399130156535264 1103 82-83 -0.01766582965628416 1103 84-85 -0.18585622925243328 1103 86-87 0.21408483834342107 1103 88-89 0.3136450202032144 1103 90-91 0.17910523339151752 1103 92-93 0.21924380879644545 1103 94-95 -0.19424987346498668 1103 96-97 -0.13747557053778792 1103 98-99 -0.5165512732782069 1103 100-101 -0.029605584933648288 1104 1 -0.03237761015123297 1104 2 -0.11013533244889118 1104 3 -0.16398267354649576 1104 4 -0.016563583757282174 1104 5 0.11888144855971916 1104 6 0.09709668401119131 1104 7 -0.1387688904324591 1104 8 0.06849389363685532 1104 9 -0.05150901647568418 1104 10-11 0.048665357556920696 1104 12-13 0.06397177823898659 1104 14-15 -0.0032142001071164827 1104 16-17 -0.2063010322145331 1104 18-19 -0.14140182071621155 1104 20-21 -0.0633036775394018 1104 22-23 -0.34702528849289394 1104 24-25 -0.11580078277544459 1104 26-27 -0.23128242144145617 1104 28-29 -0.20158598863014987 1104 30-31 -0.26568494716931923 1104 32-33 -0.1819196561747205 1104 34-35 -0.04294300002743512 1104 36-37 0.04663759033648773 1104 38-39 0.011755981853660558 1104 40-41 -0.1442855194702517 1104 42-43 -0.22622190758649907 1104 44-45 -0.18646452254353107 1104 46-47 -0.2613335988599488 1104 48-49 -0.11342424743094881 1104 50-51 -0.20058589116552383 1104 52-53 -0.1927421503947926 1104 54-55 -0.05690108787248249 1104 56-57 -0.05016018384083765 1104 58-59 -0.22427694584013125 1104 60-61 -0.2921173947158664 1104 62-63 0.04958114467229535 1104 64-65 0.4008613772657448 1104 66-67 0.23787112648734166 1104 68-69 0.10371507557913873 1104 70-71 0.22026245557881552 1104 72-73 0.306962740585508 1104 74-75 0.24152570540437068 1104 76-77 0.23020548819503972 1104 78-79 0.33228646912478865 1104 80-81 0.11482038409656425 1104 82-83 0.01928449427063228 1104 84-85 -0.009763359738958144 1104 86-87 0.008453000018914025 1104 88-89 -0.05838439866629486 1104 90-91 0.0769102822023342 1104 92-93 0.04899622681843496 1104 94-95 0.22731573009540895 1104 96-97 -0.003245495762321582 1104 98-99 0.07858374972490623 1104 100-101 0.352677311697704 1105 1 0.004245154952236874 1105 2 0.037441683518999014 1105 3 -0.07267553056524179 1105 4 0.05961257850474766 1105 5 -0.07583183021520057 1105 6 -0.2087301191094113 1105 7 0.04168362000719128 1105 8 -0.12012210668748935 1105 9 -0.016318264762773538 1105 10-11 0.0035620035469960953 1105 12-13 0.025631031449073305 1105 14-15 -0.021335902422087827 1105 16-17 0.13331708789505115 1105 18-19 0.11195233400494686 1105 20-21 -0.16611253777836765 1105 22-23 -0.004469296624527885 1105 24-25 0.07758545831247687 1105 26-27 0.08498548234577186 1105 28-29 -9.707116856816356E-5 1105 30-31 0.18029455578158604 1105 32-33 0.10108180914131282 1105 34-35 0.3986405548408385 1105 36-37 0.3073965139952577 1105 38-39 0.34730032069495564 1105 40-41 0.35476680331628074 1105 42-43 0.388572282116904 1105 44-45 0.6287550721488913 1105 46-47 0.43337099227054665 1105 48-49 0.33122749386258477 1105 50-51 0.23013521228114797 1105 52-53 0.22432993967870374 1105 54-55 0.5843433592747829 1105 56-57 0.6226291002393367 1105 58-59 0.9094982990066498 1105 60-61 0.8665472274584829 1105 62-63 0.7963703360842658 1105 64-65 0.7031899555616938 1105 66-67 0.6743535861332361 1105 68-69 0.5794027026359352 1105 70-71 0.5725413695074231 1105 72-73 0.8523202081950743 1105 74-75 0.8609714047307904 1105 76-77 0.7236521084117662 1105 78-79 0.8940447614814033 1105 80-81 0.7250608001768128 1105 82-83 0.9440306702591421 1105 84-85 0.9202953108197711 1105 86-87 0.7775694376213558 1105 88-89 0.7236969003102978 1105 90-91 0.6389907413732985 1105 92-93 0.7478281730245406 1105 94-95 0.5510615238275669 1105 96-97 0.7066624731243252 1105 98-99 0.6228914602989271 1105 100-101 0.825562449454786 1106 1 -0.03825181963429003 1106 2 -0.007737824347078259 1106 3 -0.23165092386899389 1106 4 0.13290153910714508 1106 5 0.07002671489707524 1106 6 -0.01310688393393633 1106 7 -0.2406614115915673 1106 8 -0.2328826284032317 1106 9 -0.373673381073111 1106 10-11 -0.5948311657417023 1106 12-13 -0.15749724608219395 1106 14-15 -0.16042088292497425 1106 16-17 -0.22120758603260526 1106 18-19 -0.388467862310776 1106 20-21 -0.4473415279625854 1106 22-23 -0.2457258516870624 1106 24-25 -0.41118353456099044 1106 26-27 -0.4923205550348868 1106 28-29 -0.03617680762143749 1106 30-31 -0.2245292979228708 1106 32-33 0.034983247893499936 1106 34-35 0.2469328770137551 1106 36-37 -0.00793560917267655 1106 38-39 0.012666059568154253 1106 40-41 0.23443266320602163 1106 42-43 0.08666627162880047 1106 44-45 0.07310427209510806 1106 46-47 0.43801668273713545 1106 48-49 0.6183325093258034 1106 50-51 0.3879441406124684 1106 52-53 0.6273755225750364 1106 54-55 0.5394798390381297 1106 56-57 0.5056699769369359 1106 58-59 0.35862429066995816 1106 60-61 0.26668841342055316 1106 62-63 0.629011185889297 1106 64-65 0.602497472033388 1106 66-67 0.8166892053345265 1106 68-69 0.6796951592716738 1106 70-71 0.8453395408130575 1106 72-73 0.8613023246398761 1106 74-75 1.061048720852881 1106 76-77 0.9486084079949357 1106 78-79 0.8538168469997558 1106 80-81 0.6557015155190271 1106 82-83 0.8523438341693215 1106 84-85 0.7371603101474591 1106 86-87 0.6771626875877423 1106 88-89 1.1613431286281717 1106 90-91 1.5378343611124414 1106 92-93 1.4598794033593556 1106 94-95 1.465072549778892 1106 96-97 1.3518688735546078 1106 98-99 1.3853696100905104 1106 100-101 1.598985872878206 1107 1 -0.0184128317101937 1107 2 -0.11038302273695777 1107 3 -0.021357651873593397 1107 4 -0.039985177396594906 1107 5 0.036415444051762336 1107 6 0.012731720628075038 1107 7 -0.10070549823139885 1107 8 -0.027410134632866345 1107 9 -0.10197508703438984 1107 10-11 0.1159892291212472 1107 12-13 0.1371452687730894 1107 14-15 0.08090554989081511 1107 16-17 0.0653987011055932 1107 18-19 -0.042464412052005684 1107 20-21 0.015074611581219699 1107 22-23 -0.05856848155095662 1107 24-25 -0.08821056158802065 1107 26-27 -0.025208229943842753 1107 28-29 -0.1845762709145191 1107 30-31 0.13089527558680913 1107 32-33 0.25055254059044785 1107 34-35 0.10655047333347767 1107 36-37 0.27872339025227433 1107 38-39 0.12250408908703747 1107 40-41 0.3865890749369072 1107 42-43 0.3471464326408835 1107 44-45 0.4585317208204316 1107 46-47 0.7994025366761761 1107 48-49 0.7447317809378475 1107 50-51 0.6459947444077514 1107 52-53 0.7886942881491166 1107 54-55 0.7058925283231545 1107 56-57 0.7279720662603069 1107 58-59 0.7844533111798242 1107 60-61 1.0494227434953132 1107 62-63 0.9116688435469626 1107 64-65 0.91831115805984 1107 66-67 1.088948166423279 1107 68-69 1.1816838500901525 1107 70-71 1.3213266981831957 1107 72-73 1.1140673236814713 1107 74-75 1.1467684831683904 1107 76-77 1.2698466675310662 1107 78-79 1.3940315297676307 1107 80-81 1.4197469439261567 1107 82-83 1.3819633472994717 1107 84-85 1.3403757596395032 1107 86-87 1.44963728984958 1107 88-89 1.5599438570161333 1107 90-91 1.6162798278557808 1107 92-93 1.1858905208598323 1107 94-95 1.494998752577434 1107 96-97 1.4918535390711867 1107 98-99 1.5461660448231136 1107 100-101 1.6041085487455646 1108 1 -0.14037894676141605 1108 2 0.01970628643036676 1108 3 0.02049288660814952 1108 4 -0.08312281858388104 1108 5 -0.17146477659441928 1108 6 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72-73 0.0743071834312552 2316 74-75 -0.2558811303466335 2316 76-77 -0.46378151001296075 2316 78-79 0.1598296013271181 2316 80-81 -0.4597175552371269 2316 82-83 -0.6555137425084787 2316 84-85 -0.4621184630077195 2316 86-87 -0.3407085544408055 2316 88-89 0.004616739317508944 2316 90-91 0.13059172521811746 2316 92-93 0.05801180542433926 2316 94-95 0.2788062272766112 2316 96-97 0.6003960524188656 2316 98-99 0.8845117297382465 2316 100-101 0.443071812750663 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2204.0 3 400.0 4 313.0 5 347.0 6 422.0 7 514.0 8 594.0 9 801.0 10 1165.0 11 1740.0 12 2747.0 13 3978.0 14 4599.0 15 6385.0 16 8851.0 17 12675.0 18 10278.0 19 5497.0 20 5053.0 21 5146.0 22 5465.0 23 5692.0 24 6185.0 25 6925.0 26 7609.0 27 8862.0 28 10189.0 29 12345.0 30 14933.0 31 18511.0 32 23065.0 33 30343.0 34 41416.0 35 60412.0 36 82988.0 37 113865.0 38 92103.0 39 11272.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.71834837998917 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19.86103105987832 26.39369196285623 34.00696445725264 19.73831252001281 20-21 21.254195581121508 25.059096356078257 33.970098404538476 19.716609658261763 22-23 20.798510527086748 25.39492083973358 33.90527983248233 19.90128880069735 24-25 20.556482261147416 25.435667244034487 34.213445733294485 19.79440476152361 26-27 20.286297029766374 25.706537139527175 34.21764209589341 19.789523734813034 28-29 19.737217091696234 26.044295082807118 34.48063030753269 19.73785751796396 30-31 19.714165178360577 26.070159456220587 34.20155413578315 20.01412122963569 32-33 19.671658709497688 26.19189425450773 33.86239701309971 20.274050022894873 34-35 20.23852411900427 26.131496487865647 33.466469404322154 20.163509988807924 36-37 20.422767455486788 25.818143396419735 33.47976293772793 20.27932621036555 38-39 20.1326348060306 26.11560920359082 33.700527097282865 20.051228893095708 40-41 20.009029193592156 26.219561491770065 33.669766849892056 20.101642464745723 42-43 19.945295921528384 26.60360035124597 32.88776538562213 20.563338341603515 44-45 19.99803059463233 26.88470492543479 32.45556028782139 20.66170419211149 46-47 20.311811996932157 27.050419266567715 31.58117215307833 21.056596583421797 48-49 20.725097394331353 27.315327057610368 30.797744838861018 21.16183070919726 50-51 20.711298913509044 27.652559870687483 30.671212993378255 20.96492822242522 52-53 20.83816408101564 27.8363961874992 30.279019715855952 21.046420015629202 54-55 20.209469856483846 27.59271386917181 30.525003642745176 21.67281263159917 56-57 20.778377686079413 28.20506457797185 29.834742828783323 21.181814907165414 58-59 20.853145431538913 27.308001059668797 30.241378785974916 21.597474722817374 60-61 20.89221830055944 27.08876563662993 30.96512921478707 21.053886848023563 62-63 21.04803570342339 27.18007946188743 30.83582252017762 20.93606231451156 64-65 20.66780314853498 27.40730069549489 30.811624128630537 21.11327202733959 66-67 20.505405974303915 27.594679583272974 30.553178956197563 21.34673548622555 68-69 20.485449404073478 27.593106765732966 30.626405175608433 21.295038654585124 70-71 20.46211092230892 27.809895493586108 30.175836823683746 21.552156760421227 72-73 20.601945601905584 27.478945590700043 30.121692164606177 21.797416642788196 74-75 20.305095083085146 27.85355307948496 30.078094672481814 21.763257164948083 76-77 20.45042304705948 27.546407101747423 30.267035948994213 21.736133902198883 78-79 20.255294352130747 27.522706661234302 30.36557184285958 21.85642714377537 80-81 20.058477613063257 27.840578468936673 30.250919065414728 21.85002485258535 82-83 20.183080904087024 27.793816677378537 29.978637626431393 22.044464792103042 84-85 20.093567906768264 27.3343952103477 29.678315297432285 22.89372158545175 86-87 20.420612476697343 27.195732722014597 29.812994418568167 22.570660382719893 88-89 20.436788877371743 26.75834137087834 29.902068683990414 22.90280106775951 90-91 20.04048826056573 26.859934472736064 30.428072501042625 22.67150476565558 92-93 20.188003115242356 26.955811103613826 30.412353392708635 22.443832388435183 94-95 19.89657236892657 26.867092052352536 30.257744666137338 22.978590912583563 96-97 20.0610907484177 26.86712201900441 30.045649965700488 23.0261372668774 98-99 20.21756705916255 26.7863632361299 29.632105149407256 23.363964555300296 100-101 20.483074233906596 26.302967051260705 30.349768219128965 22.864190495703728 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1573.0 1 1019.0 2 558.5 3 679.0 4 582.0 5 549.0 6 1015.5 7 2229.5 8 3613.0 9 4004.5 10 3181.5 11 2382.0 12 2341.5 13 2410.0 14 2395.0 15 2347.5 16 2314.0 17 2348.0 18 2412.5 19 2483.0 20 2603.5 21 2828.0 22 3121.5 23 3475.5 24 3894.0 25 4446.5 26 5148.0 27 5834.0 28 6516.5 29 7267.0 30 8082.0 31 9117.0 32 10315.0 33 11328.0 34 12504.0 35 13454.5 36 14505.0 37 15858.5 38 16512.5 39 16449.0 40 16648.5 41 17840.5 42 19235.5 43 20358.5 44 21248.0 45 21769.0 46 21864.0 47 23124.0 48 28352.0 49 28076.0 50 22467.5 51 19897.5 52 17883.0 53 17277.0 54 16937.0 55 15390.5 56 13698.0 57 12063.5 58 10870.0 59 9919.0 60 8659.0 61 7346.0 62 5942.5 63 4389.0 64 3095.5 65 2314.5 66 1669.5 67 1234.5 68 888.0 69 586.5 70 428.5 71 343.5 72 288.5 73 236.5 74 183.0 75 150.0 76 100.0 77 57.5 78 40.5 79 30.5 80 22.0 81 14.5 82 11.0 83 8.0 84 6.0 85 4.5 86 1.5 87 1.0 88 1.5 89 1.5 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22192362567923168 2 0.20163255263296645 3 0.1941232578835612 4 0.20003483034585892 5 0.19731870245777622 6 0.21489364761595867 7 0.21633159767435545 8 0.20898207515366093 9 0.20259118600523093 10-11 0.21409478647240496 12-13 0.2177695477327522 14-15 0.20323027492007395 16-17 0.2144942170441818 18-19 0.20626594726557818 20-21 0.20378947772056155 22-23 0.19819744971568531 24-25 0.1944428023409827 26-27 0.20131300817554493 28-29 0.2088223029249502 30-31 0.20762401120961957 32-33 0.20722458063784271 34-35 0.21441433092982642 36-37 0.1989164247448837 38-39 0.19867676640181756 40-41 0.19979517200279284 42-43 0.20091357760376807 44-45 0.21361546978627266 46-47 0.21537296430209096 48-49 0.2185684088763059 50-51 0.21689080047484305 52-53 0.22671679254055419 54-55 0.21760977550404145 56-57 0.20107334983247882 58-59 0.18653407701980057 60-61 0.18469669638962694 62-63 0.1899691799370817 64-65 0.18469669638962694 66-67 0.18621453256237908 68-69 0.1903686105088586 70-71 0.1905283827375693 72-73 0.19172667445289993 74-75 0.1918065605672553 76-77 0.20067391926070194 78-79 0.1980376774869746 80-81 0.19292496616823057 82-83 0.19084792719499083 84-85 0.1934841689687182 86-87 0.19659972742857781 88-89 0.19468246068404882 90-91 0.20219175543345408 92-93 0.1950020051414703 94-95 0.1922059911390322 96-97 0.19915608308794983 98-99 0.20067391926070194 100-101 0.1858151019906022 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 625891.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.38969475519942 #Duplication Level Percentage of deduplicated Percentage of total 1 77.06822659130007 40.37580866442754 2 12.69825420178642 13.305153231110381 3 4.331446805743946 6.807695280039266 4 1.9051391890061424 3.9923864231279986 5 1.0358668910770297 2.7134375115271494 6 0.6412669938152927 2.0157469237540524 7 0.4176810190797006 1.5317526766238299 8 0.29703867147879703 1.2449412263411308 9 0.22408363255466576 1.0565705798257685 >10 1.2038009770387053 11.907989024352016 >50 0.1134536233000113 4.001200280164807 >100 0.057641747951389044 5.40885747943433 >500 0.003659793520722896 1.3804953510076496 >1k 0.001829896760361448 1.8039111704718014 >5k 6.099655867871493E-4 2.454054177792418 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8799 1.4058358404258888 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6560 1.0481058203425198 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4674 0.7467753969940453 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1753 0.2800807169299447 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1397 0.2232018035089177 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1257 0.20083369148941269 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1193 0.1906082688519247 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1016 0.16232858437012196 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 945 0.1509847561316587 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 904 0.14443409475451796 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 888 0.14187773909514595 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 836 0.13356958320218695 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 819 0.13085345531410422 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 640 0.10225422637487996 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 638 0.10193468191745847 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.5977222871074997E-4 0.0 14-15 0.0 0.0 0.0 1.5977222871074997E-4 0.0 16-17 0.0 0.0 0.0 2.3965834306612495E-4 0.0 18-19 0.0 0.0 0.0 3.9943057177687486E-4 0.0 20-21 0.0 0.0 0.0 4.7931668613224984E-4 0.0 22-23 0.0 0.0 0.0 4.7931668613224984E-4 0.0 24-25 0.0 0.0 0.0 0.0010385194866198747 0.0 26-27 0.0 0.0 0.0 0.0019172667445289996 0.0 28-29 0.0 0.0 0.0 0.004233964060834874 0.0 30-31 0.0 0.0 0.0 0.007908725321182123 0.0 32-33 0.0 0.0 0.0 0.013900183897835247 0.0 34-35 0.0 0.0 0.0 0.019971528588843747 0.0 36-37 0.0 0.0 0.0 0.025164126021943116 0.0 38-39 0.0 0.0 0.0 0.030596381798108616 0.0 40-41 0.0 0.0 0.0 0.04050225997817511 0.0 42-43 0.0 0.0 0.0 0.057917432907646865 0.0 44-45 0.0 0.0 0.0 0.08851381470575548 0.0 46-47 0.0 0.0 0.0 0.12046826044790547 0.0 48-49 0.0 0.0 0.0 0.19867676640181758 0.0 50-51 0.0 0.0 0.0 0.2721719916087626 0.0 52-53 0.0 0.0 0.0 0.3559725255675509 0.0 54-55 0.0 0.0 0.0 0.4917789199716884 0.0 56-57 0.0 0.0 0.0 0.6342158618673219 0.0 58-59 0.0 0.0 0.0 0.8103647440209238 0.0 60-61 0.0 0.0 0.0 1.0097604854519397 0.0 62-63 0.0 0.0 0.0 1.2653161652747844 0.0 64-65 0.0 0.0 0.0 1.4683866679661475 0.0 66-67 0.0 0.0 0.0 1.7406385456892655 0.0 68-69 0.0 0.0 0.0 2.0811131650718737 0.0 70-71 0.0 0.0 0.0 2.34026372004071 0.0 72-73 0.0 0.0 0.0 2.7341821499270638 0.0 74-75 0.0 0.0 0.0 3.2185316612637025 0.0 76-77 0.0 0.0 0.0 3.5931176514760557 0.0 78-79 0.0 0.0 0.0 3.9659461471725908 0.0 80-81 0.0 0.0 0.0 4.490158829572561 0.0 82-83 0.0 0.0 0.0 5.09577546250066 0.0 84-85 0.0 0.0 0.0 5.726795879793766 0.0 86-87 0.0 0.0 0.0 6.54978262988284 0.0 88-89 0.0 0.0 0.0 7.506179190945389 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCTA 50 0.00161479 38.00221 9 GACGCGC 25 0.0016173157 37.993084 60-61 AAGACCG 55 0.0025729348 34.5613 5 GTATCAA 8135 0.0 29.906828 1 GGTATCA 5585 0.0 28.757524 1 TGCGATT 35 0.008397657 27.139006 56-57 GTACATG 4600 0.0 25.10189 1 ATAGTAC 135 1.3391835E-5 24.635008 3 TATCAAC 9895 0.0 24.343363 2 TACATGG 4710 0.0 24.108236 2 AGTACTT 5760 0.0 23.998793 12-13 ATCAACG 10025 0.0 23.980297 3 GAGTACT 5695 0.0 23.939056 12-13 TCAACGC 10045 0.0 23.936384 4 CAACGCA 10115 0.0 23.77264 5 GTATAGG 160 1.8511855E-6 23.758991 1 AACGCAG 10235 0.0 23.439978 6 CTGGACG 250 3.8016879E-10 22.80863 4 CCTATAC 125 2.250972E-4 22.804976 3 ACATGGG 4710 0.0 22.696037 3 >>END_MODULE