FastQCFastQC Report
Thu 26 May 2016
SRR937416_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937416_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625891
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78291.2508567785764615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62681.0014523295589808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46530.7434201801911196No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20250.32353876313926866No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14960.23901925415128192No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14930.23853993746514968No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13380.21377524201498344No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12790.20434868052104918No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12440.19875665251617292No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9960.15913313979590696No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT7480.11950962707564096No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG405.387113E-447.4985245
TATAGGG608.28794E-539.58212
GTAAGGT1800.036.9432954
ATATACG600.003950190331.6656824
AGGTAAG1859.094947E-1230.8098532
TAAGGTG2200.030.2263325
GGTGTCG650.00583839229.2345338
GTATCAA91800.027.4922081
GGTAAGG2207.6397555E-1125.9082833
GGTATCA65550.025.667781
GAGTACT59450.024.37235312-13
AGTACTT60450.024.00846512-13
GGTACTC1000.001653106523.749263
GTACATG48200.023.6658421
ATGGGAG11600.023.339795
GTACTTT63600.022.8157214-15
AAGGGTA1252.2547836E-422.7992924
TATCAAC111450.022.5879022
ATCAACG112350.022.406963
TACATGG49350.022.32962
Produced by FastQC (version 0.11.5)