##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937416_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 625891 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19080319097095 33.0 31.0 34.0 31.0 34.0 2 32.37726696821012 34.0 31.0 34.0 31.0 34.0 3 32.40178721215036 34.0 31.0 34.0 30.0 34.0 4 36.07018634235035 37.0 35.0 37.0 35.0 37.0 5 36.006686467771544 37.0 35.0 37.0 35.0 37.0 6 36.06619203663258 37.0 35.0 37.0 35.0 37.0 7 36.0076834464787 37.0 35.0 37.0 35.0 37.0 8 35.99749317373153 37.0 35.0 37.0 35.0 37.0 9 37.67787362336254 39.0 37.0 39.0 35.0 39.0 10-11 37.604483847826536 39.0 37.0 39.0 35.0 39.0 12-13 37.500004793166866 39.0 37.0 39.0 35.0 39.0 14-15 38.598355783994336 40.0 38.0 41.0 34.0 41.0 16-17 38.60416669995255 40.0 38.0 41.0 34.5 41.0 18-19 38.68544522928114 40.0 38.0 41.0 34.5 41.0 20-21 38.634713552359756 40.0 38.0 41.0 34.0 41.0 22-23 38.485375249044964 40.0 38.0 41.0 34.0 41.0 24-25 38.38896628965746 40.0 38.0 41.0 34.0 41.0 26-27 38.27985463922632 40.0 38.0 41.0 34.0 41.0 28-29 38.11731595437544 40.0 37.0 41.0 34.0 41.0 30-31 38.065409152711894 40.0 37.0 41.0 34.0 41.0 32-33 37.88897667485233 40.0 36.5 41.0 33.5 41.0 34-35 37.698943586023766 40.0 36.0 41.0 33.0 41.0 36-37 37.46948749862196 40.0 36.0 41.0 33.0 41.0 38-39 37.28021812104664 40.0 35.0 41.0 33.0 41.0 40-41 37.02525120188659 40.0 35.0 41.0 32.5 41.0 42-43 36.71253061635333 39.0 35.0 41.0 31.5 41.0 44-45 36.24498514917134 39.0 35.0 41.0 30.5 41.0 46-47 35.6237843330548 39.0 35.0 41.0 28.0 41.0 48-49 35.09014189371632 39.0 35.0 41.0 25.0 41.0 50-51 34.754020268704934 39.0 35.0 40.5 23.5 41.0 52-53 34.464389166803805 38.5 34.5 40.0 23.0 41.0 54-55 34.21195463746882 38.0 34.0 40.0 21.5 41.0 56-57 33.934961518858714 38.0 34.0 40.0 20.0 41.0 58-59 33.64573703727965 38.0 34.0 40.0 18.5 41.0 60-61 33.30530395867651 37.0 33.0 40.0 16.0 41.0 62-63 32.92724771565656 37.0 33.0 40.0 11.0 41.0 64-65 32.50849748598398 36.0 32.5 39.0 7.5 41.0 66-67 32.0943327192754 36.0 32.0 39.0 4.5 40.5 68-69 31.653042622437454 35.0 31.5 39.0 2.0 40.0 70-71 31.22361641244242 35.0 31.0 38.0 2.0 40.0 72-73 30.761202030385483 35.0 31.0 37.0 2.0 39.5 74-75 30.28983321377045 35.0 30.0 37.0 2.0 39.0 76-77 29.03800342232114 33.5 28.5 35.5 2.0 37.5 78-79 29.5908568744398 34.0 30.0 36.0 2.0 38.0 80-81 29.38100803494538 34.0 30.0 35.5 2.0 37.0 82-83 29.03516506867809 34.0 29.0 35.0 2.0 37.0 84-85 28.68234405032186 34.0 29.0 35.0 2.0 36.0 86-87 28.271965885433726 34.0 29.0 35.0 2.0 36.0 88-89 27.810400692772383 34.0 28.0 35.0 2.0 35.5 90-91 27.30815030093099 33.0 26.5 35.0 2.0 35.0 92-93 26.998578027164477 33.0 26.0 35.0 2.0 35.0 94-95 26.58198632030178 33.0 25.0 35.0 2.0 35.0 96-97 26.130694481946538 33.0 24.5 35.0 2.0 35.0 98-99 25.44630374937489 33.0 21.5 35.0 2.0 35.0 100-101 23.232139461982996 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.12134797014138599 1101 2 -0.07573949865900431 1101 3 -0.1398892049435716 1101 4 -0.08786852762845143 1101 5 -0.021034272122442133 1101 6 -0.19618304034415956 1101 7 -0.1795564896101638 1101 8 -0.17385485173355875 1101 9 -0.2366472265406756 1101 10-11 -0.13439435796859556 1101 12-13 -0.2595373292902323 1101 14-15 -0.42363883027568505 1101 16-17 -0.2708832414139124 1101 18-19 -0.01018667922730998 1101 20-21 -0.19117620875081798 1101 22-23 -0.20795430202630172 1101 24-25 -0.24917538199752443 1101 26-27 -0.3948745115931587 1101 28-29 -0.5439881857849045 1101 30-31 -0.4016870840018001 1101 32-33 -0.3229336237991802 1101 34-35 -0.34581463026822234 1101 36-37 -0.16082616186833576 1101 38-39 -0.21488753270050154 1101 40-41 -0.2565051825706419 1101 42-43 -0.4104973998570003 1101 44-45 -0.24613725578297618 1101 46-47 -0.3298997116546758 1101 48-49 -0.5545587818525703 1101 50-51 -0.577078273464906 1101 52-53 -0.4968887003452167 1101 54-55 -0.4547083333574591 1101 56-57 -0.28066710821394736 1101 58-59 -0.2617067762757799 1101 60-61 -0.36692952749341146 1101 62-63 -0.38073262512501316 1101 64-65 -0.5275253931474992 1101 66-67 -0.4356794554604271 1101 68-69 -0.38165353797697676 1101 70-71 -0.35716052973170065 1101 72-73 -0.3171326950312583 1101 74-75 -0.5734516483163894 1101 76-77 -0.3425933494671085 1101 78-79 -0.33823779477326 1101 80-81 -0.17642126377413803 1101 82-83 -0.11143660225760144 1101 84-85 -0.22230702155144044 1101 86-87 -0.3415511977160932 1101 88-89 -0.1583063850479398 1101 90-91 -0.15394477995912936 1101 92-93 -0.035721829783224024 1101 94-95 0.1255886433819775 1101 96-97 0.1374483702980669 1101 98-99 0.09914139724510207 1101 100-101 0.4818465556707494 1102 1 -0.30476606876539236 1102 2 -0.19413932748249607 1102 3 -0.1830865846262384 1102 4 -0.11391533782003194 1102 5 -0.21798189869430473 1102 6 -0.1785098887212726 1102 7 -0.2804313003284449 1102 8 -0.3507550755797624 1102 9 -0.4191929833383625 1102 10-11 -0.23686571882185348 1102 12-13 -0.24249785993740858 1102 14-15 -0.3567380303546912 1102 16-17 -0.3329758742403044 1102 18-19 -0.46430026471680463 1102 20-21 -0.22337055992601051 1102 22-23 -0.29942469178204334 1102 24-25 -0.3876534746961866 1102 26-27 -0.2609967875823571 1102 28-29 -0.41777842872387794 1102 30-31 -0.5285593270724434 1102 32-33 -0.44508205358389574 1102 34-35 -0.47295022181474167 1102 36-37 -0.5185480329592664 1102 38-39 -0.4334585380333067 1102 40-41 -0.38094145223947606 1102 42-43 -0.39045411195127855 1102 44-45 -0.23373518849744102 1102 46-47 -0.1630293614012075 1102 48-49 -0.10411561574552053 1102 50-51 -0.4490209622528454 1102 52-53 -0.150060815033477 1102 54-55 -0.19962035850723936 1102 56-57 -0.4357114659728438 1102 58-59 -0.25410876126492354 1102 60-61 -0.3210904334121736 1102 62-63 -0.27971173829902085 1102 64-65 -0.3371758803421798 1102 66-67 -0.43263243129820594 1102 68-69 -0.3341088510324788 1102 70-71 -0.36270319218479585 1102 72-73 -0.2767292780847832 1102 74-75 -0.308782315246404 1102 76-77 -0.23119488676383781 1102 78-79 -0.4383945694128002 1102 80-81 -0.18357693564193767 1102 82-83 0.013044930430528012 1102 84-85 -0.3358842814105465 1102 86-87 -0.0342008948653465 1102 88-89 -0.11753263430727401 1102 90-91 -0.1615818857593787 1102 92-93 -0.23856805073259224 1102 94-95 -0.15111859540925465 1102 96-97 -0.18890622842469185 1102 98-99 -0.15100879368253928 1102 100-101 0.0996867689830161 1103 1 -0.004073479447072259 1103 2 -0.04302128700818031 1103 3 0.0014957957861909676 1103 4 -0.062094858508828565 1103 5 -0.11081072847540696 1103 6 -0.13248981492195355 1103 7 -0.12761601964118796 1103 8 -0.10718488935179238 1103 9 -0.07817982822525948 1103 10-11 -0.06541562945547952 1103 12-13 -0.10047140557191625 1103 14-15 -0.16791134824910614 1103 16-17 -0.14741574893520237 1103 18-19 -0.11386340253319105 1103 20-21 -0.20115869437914569 1103 22-23 -0.06257560082054425 1103 24-25 -0.07626274669664923 1103 26-27 -0.11884601307387754 1103 28-29 -0.16102550269616245 1103 30-31 -0.4923263114861811 1103 32-33 -0.12586516238023648 1103 34-35 -0.18945825517818093 1103 36-37 -0.43113836025155905 1103 38-39 -0.4318874197045517 1103 40-41 -0.3085462895542648 1103 42-43 -0.24781266325525309 1103 44-45 -0.15760081223621825 1103 46-47 -0.23712356604056595 1103 48-49 -0.2689226852975324 1103 50-51 -0.02252359519732039 1103 52-53 0.10853769456620199 1103 54-55 -0.08368732081961383 1103 56-57 -0.19436763129100854 1103 58-59 -0.29336329684810636 1103 60-61 0.028010137379446576 1103 62-63 0.11183965696936582 1103 64-65 -0.1626249943659701 1103 66-67 0.08083678535276562 1103 68-69 0.06964874030379065 1103 70-71 -0.1508052139786571 1103 72-73 -0.043919090319327836 1103 74-75 0.1945653199082109 1103 76-77 0.16723435525565122 1103 78-79 0.11266016988725625 1103 80-81 0.2508528973486648 1103 82-83 0.5151541687984853 1103 84-85 0.46085528770904816 1103 86-87 0.4155863795566539 1103 88-89 0.20146564563046 1103 90-91 0.4435701331516988 1103 92-93 0.5181854758755229 1103 94-95 0.6357600441079754 1103 96-97 0.498107301450581 1103 98-99 0.6504038915364525 1103 100-101 1.1594973699477862 1104 1 -0.12165069442618659 1104 2 -0.03816545393529225 1104 3 -0.12108874115594404 1104 4 0.032168079802438854 1104 5 -0.07634632358791293 1104 6 -0.014395354092755497 1104 7 -0.07205781037635006 1104 8 -0.14129854969186795 1104 9 0.08875683440625437 1104 10-11 -0.06746482376659912 1104 12-13 -0.21280382951698584 1104 14-15 -0.14308354744471075 1104 16-17 -0.13962771874115276 1104 18-19 -0.11504324168202373 1104 20-21 -0.23528395521981338 1104 22-23 -0.2374763483022022 1104 24-25 -0.19813828833766678 1104 26-27 -0.3176301441349665 1104 28-29 -0.2647530847287598 1104 30-31 -0.23261498653159407 1104 32-33 -0.07978524448449065 1104 34-35 0.01675497294088757 1104 36-37 0.13622233479585333 1104 38-39 -0.04545017909515536 1104 40-41 0.0014620764538122444 1104 42-43 -0.17755047829414394 1104 44-45 0.09314127861071597 1104 46-47 0.23817760342636518 1104 48-49 0.19131762248421325 1104 50-51 0.34345718618509835 1104 52-53 0.32747394230922566 1104 54-55 0.5831742797492225 1104 56-57 0.2797059322599438 1104 58-59 0.2759210341510183 1104 60-61 0.30881185479943696 1104 62-63 0.4355482141809901 1104 64-65 0.42727752048607925 1104 66-67 0.49860015424732396 1104 68-69 0.6610586378509034 1104 70-71 0.35092326061010937 1104 72-73 0.6904587884813722 1104 74-75 0.6102774473444867 1104 76-77 0.6588799476795693 1104 78-79 0.4729867308028517 1104 80-81 0.6044402498005326 1104 82-83 0.6044350332950863 1104 84-85 0.44223648425291984 1104 86-87 0.3351614736913042 1104 88-89 0.5131103646049198 1104 90-91 0.4438041903107752 1104 92-93 0.6258920296124515 1104 94-95 0.6213931653113711 1104 96-97 0.8534538356986694 1104 98-99 0.8945091312778608 1104 100-101 1.150801771177715 1105 1 -0.05362933440441253 1105 2 -0.00827561326669013 1105 3 -0.09454364359369549 1105 4 -0.058077722617362326 1105 5 -0.02989059292805507 1105 6 -0.11957811317278555 1105 7 -0.1088557763091984 1105 8 -0.16400950017808213 1105 9 -0.09383718038591127 1105 10-11 -0.1498367244494503 1105 12-13 -0.23248134415566568 1105 14-15 -0.21725358781329618 1105 16-17 -0.36203141620262613 1105 18-19 -0.1608864934094143 1105 20-21 -0.170091871401425 1105 22-23 -0.09755749090120247 1105 24-25 -0.137339980690804 1105 26-27 -0.23109251794693364 1105 28-29 -0.23212880797516533 1105 30-31 -0.04850570175640456 1105 32-33 -0.08410517567833864 1105 34-35 0.09646193869419051 1105 36-37 0.01055338617457835 1105 38-39 -0.040413517235656116 1105 40-41 -0.03078139188551887 1105 42-43 0.017857812035352083 1105 44-45 0.1650597711306503 1105 46-47 -0.011356883626149283 1105 48-49 0.22538187630243556 1105 50-51 0.08268016326709215 1105 52-53 0.2165920884817396 1105 54-55 0.28107809594182953 1105 56-57 0.4321393414653727 1105 58-59 0.32360461855727607 1105 60-61 0.44898337785934217 1105 62-63 0.35972172960734383 1105 64-65 0.39560743667927056 1105 66-67 0.32346158631862565 1105 68-69 0.4705537988329489 1105 70-71 0.3653114477303845 1105 72-73 0.2176482196723306 1105 74-75 0.19140629090317773 1105 76-77 0.4904402775783865 1105 78-79 0.2659550961935935 1105 80-81 0.051880000986397334 1105 82-83 -0.12382196460250583 1105 84-85 -0.03496062102395925 1105 86-87 0.1999281525206733 1105 88-89 0.34969577283733244 1105 90-91 0.6051887691627336 1105 92-93 0.6471289764266928 1105 94-95 0.6187028914189021 1105 96-97 0.5472592556304434 1105 98-99 0.8008736995888093 1105 100-101 0.6627118077237348 1106 1 -0.06513935188457509 1106 2 0.0454153911705788 1106 3 -0.07510031967276376 1106 4 -0.04245986595472573 1106 5 -0.08026036568588779 1106 6 -0.1383625692706758 1106 7 -0.06767653736876866 1106 8 -0.10328609020094603 1106 9 -0.07165753805701058 1106 10-11 -0.16960275914085088 1106 12-13 -0.3140364066808772 1106 14-15 -0.22826273128512753 1106 16-17 -0.08333032705377263 1106 18-19 -0.2595788424749017 1106 20-21 -0.20522700653656045 1106 22-23 -0.15838847113517573 1106 24-25 -0.11103905289733973 1106 26-27 -0.14297230818493034 1106 28-29 -0.23275406009295097 1106 30-31 -0.19140270323548947 1106 32-33 -0.11952268273764588 1106 34-35 0.03745629125570815 1106 36-37 0.027757904125358834 1106 38-39 0.10002617618911813 1106 40-41 0.07071450699844917 1106 42-43 -0.17239363343997383 1106 44-45 0.13850672554775656 1106 46-47 0.3585839527326016 1106 48-49 0.3698957932044138 1106 50-51 0.4838600745216368 1106 52-53 0.797505091036534 1106 54-55 0.5071336290869155 1106 56-57 0.24495700988005353 1106 58-59 0.3675100911940987 1106 60-61 0.6035551137743624 1106 62-63 0.4630967464152036 1106 64-65 0.64422919276371 1106 66-67 0.5514365762339146 1106 68-69 0.8245170904828036 1106 70-71 0.956564641222382 1106 72-73 1.1436129904132208 1106 74-75 0.9816308438142975 1106 76-77 0.8718373462888884 1106 78-79 0.9061695643931351 1106 80-81 0.7856133612122953 1106 82-83 0.8591348728319446 1106 84-85 0.8128308271406262 1106 86-87 0.8273215907350071 1106 88-89 1.0559886328615349 1106 90-91 1.0525297668768623 1106 92-93 1.072633212005556 1106 94-95 0.94038838288051 1106 96-97 0.9601744095903726 1106 98-99 1.3375517955952638 1106 100-101 1.9454333352213844 1107 1 -0.05848801970132911 1107 2 -0.2712321073918105 1107 3 -0.15348828210327525 1107 4 -0.17033028956983287 1107 5 -0.14474186846526038 1107 6 -0.25012220124307305 1107 7 -0.22672388258632026 1107 8 -0.18403172945722446 1107 9 -0.03727954304071801 1107 10-11 -0.19602599088323558 1107 12-13 -0.09265917838875737 1107 14-15 -0.2254546040089167 1107 16-17 -0.3171811992025084 1107 18-19 -0.29022384918373234 1107 20-21 -0.14030463735886656 1107 22-23 -0.3284191401020067 1107 24-25 -0.20030970652970126 1107 26-27 -0.24441950005765278 1107 28-29 -0.0971732665334315 1107 30-31 -0.1964918349203728 1107 32-33 -0.2500335127165556 1107 34-35 -0.10631407485507793 1107 36-37 -0.23484991646117948 1107 38-39 -0.2544735362893249 1107 40-41 -0.07213579010717552 1107 42-43 -0.09357438866329204 1107 44-45 -0.2174928910901599 1107 46-47 -0.3855841515418845 1107 48-49 -0.37040514603269514 1107 50-51 -0.45322541522342874 1107 52-53 -0.6651746099410403 1107 54-55 -0.4755192365446774 1107 56-57 -0.42744571199075665 1107 58-59 -0.39578778114633195 1107 60-61 -0.25210426901268335 1107 62-63 -0.3020412639355783 1107 64-65 -0.2972553769123465 1107 66-67 -0.4629203338676753 1107 68-69 -0.009812984272794978 1107 70-71 0.039562532730911926 1107 72-73 0.1274926147183777 1107 74-75 0.2613941177265069 1107 76-77 0.17080706068413676 1107 78-79 0.09302982891297518 1107 80-81 0.2500804879134648 1107 82-83 -0.08329851800570154 1107 84-85 0.05022779858014914 1107 86-87 0.17908026430219337 1107 88-89 0.20122535894932625 1107 90-91 0.35190201146187405 1107 92-93 0.39313829155318913 1107 94-95 0.14202246376991212 1107 96-97 0.06904924186946104 1107 98-99 0.24104518259928653 1107 100-101 0.531244146032197 1108 1 -0.05943364617886715 1108 2 -0.14412414836895948 1108 3 -0.1719805332196458 1108 4 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0.46061876022798387 2316 74-75 0.564617482138015 2316 76-77 0.29700436724242607 2316 78-79 0.4971656166251428 2316 80-81 0.5244609166328829 2316 82-83 0.15487583851673037 2316 84-85 0.5087464056404727 2316 86-87 0.2656528995719327 2316 88-89 0.722159297908604 2316 90-91 0.5117235111829679 2316 92-93 0.6516568986135134 2316 94-95 0.7586771772929026 2316 96-97 0.6593393048226837 2316 98-99 0.9848415010310347 2316 100-101 0.8312441460321942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 5.0 5 24.0 6 44.0 7 97.0 8 223.0 9 445.0 10 762.0 11 1323.0 12 2139.0 13 2973.0 14 3990.0 15 5579.0 16 8164.0 17 11979.0 18 10418.0 19 5444.0 20 4140.0 21 4248.0 22 4345.0 23 4629.0 24 5028.0 25 5803.0 26 6561.0 27 7748.0 28 9628.0 29 11705.0 30 14309.0 31 17854.0 32 21899.0 33 28703.0 34 39891.0 35 58789.0 36 85639.0 37 126580.0 38 104863.0 39 9912.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.1059700371059 19.603937286073535 15.280404535840525 20.009688140980035 2 15.751624484135416 22.394154892784847 39.092749376488875 22.76147124659086 3 19.25686740982056 27.23317638374733 31.09503092391487 22.414925282517242 4 12.42884144363795 17.93794766181332 37.10102877338067 32.53218212116806 5 12.735528752362676 39.169871492732625 32.82327734274516 15.271322412159538 6 29.475579613702706 37.58881338763459 17.26482726225493 15.670779736407777 7 26.81185701663708 36.06027247555884 19.96401929409434 17.163851213709734 8 27.41004424093013 33.32880645351986 19.51969272604974 19.741456579500262 9 28.10649138588029 16.28238782791253 20.19185449223587 35.41926629397131 10-11 24.795011903050057 27.828691942673633 26.198852833565006 21.177443320711305 12-13 27.094529549222074 25.981825081387683 27.28898120593581 19.63466416345443 14-15 23.349033617674646 26.130827891757512 27.971004535933574 22.549133954634275 16-17 20.39907907287371 29.941235774280184 28.062873567442253 21.59681158540385 18-19 20.25280759748902 27.08115310812905 33.098335013604604 19.567704280777324 20-21 21.725508115630358 25.635853527211605 33.11774733939296 19.520891017765074 22-23 21.064681855256502 26.189333050001878 32.8911871401604 19.854797954581223 24-25 21.14884863493839 26.31263605679934 33.156198198558045 19.38231710970422 26-27 20.899153044709205 26.44853959727683 33.19773953528501 19.45456782272895 28-29 20.13259931567313 26.840616389492716 33.453166429796575 19.573617865037576 30-31 20.148301056577125 26.89518428986609 33.182321465946835 19.774193187609942 32-33 20.116282228055685 26.996393940797997 32.89957836108843 19.987745470057884 34-35 20.73867494499841 26.747309036237937 32.708890206122156 19.805125812641496 36-37 20.94126613100364 26.602475510911646 32.486966580442925 19.969291777641793 38-39 20.664606663481862 26.770881098725173 32.717382794712805 19.847129443080163 40-41 20.442872994557355 27.202122415981712 32.534524809052066 19.820479780408874 42-43 20.406726795248836 27.58300836255474 31.77515138005722 20.235113462139207 44-45 20.628129041741328 27.723938931809954 31.210011974938105 20.43792005151061 46-47 20.950373148040153 27.984824833717052 30.363433888648345 20.70136812959445 48-49 21.35028303650316 28.214337640260045 29.73912390496109 20.696255418275705 50-51 21.40452570815046 28.580535588465082 29.344486500045537 20.67045220333892 52-53 21.349564061473963 29.01935001461916 28.893848928966865 20.737236994940016 54-55 20.540078064710947 28.716501755097934 29.238158081838534 21.50526209835259 56-57 21.161911578853186 29.30038936492137 28.60865550071818 20.929043555507267 58-59 21.286134486675795 28.437140013197187 28.930995972142114 21.345729527984904 60-61 21.118932849329994 28.246531744345262 29.733851421413632 20.90068398491111 62-63 21.387030649106634 28.43626126593928 29.59548867135012 20.581219413603964 64-65 20.94230465049026 28.60585948671574 29.57871258733549 20.873123275458504 66-67 20.755690687356104 28.77521804914913 29.174249190354228 21.29484207314053 68-69 20.86305762504973 28.775697365835267 29.163464564916254 21.19778044419875 70-71 20.843725185375728 29.14580973364372 28.88146658124178 21.12899849973877 72-73 20.901163301597244 28.84751498264075 28.6728839366599 21.57843777910211 74-75 20.768472465652966 29.302706062237675 28.345670412260283 21.58315105984908 76-77 20.78924285538536 28.857900177506945 28.905592187777106 21.447264779330585 78-79 20.584255085949472 28.860696191509383 28.77961178543868 21.775436937102466 80-81 20.276773431795633 29.281296583590432 28.70795394086191 21.733976043752026 82-83 20.42555401108821 29.24946875649076 28.357379092172746 21.96759814024829 84-85 20.22497581442092 28.954312222844447 28.21712139283372 22.603590569900916 86-87 20.5022120465065 28.498252890679048 28.19316781995587 22.80636724285858 88-89 20.561886973929965 28.43721989931154 28.121669747607807 22.87922337915068 90-91 20.1560953317605 28.52083334997775 28.510127661342317 22.81294365691943 92-93 20.01701610938243 28.518360222247964 28.51196919524987 22.95265447311974 94-95 20.082889832255137 28.412535089975734 28.205390395452245 23.29918468231689 96-97 20.341001867737354 28.439057279941714 27.953429590775393 23.266511261545542 98-99 20.121091468875147 28.548204518202464 27.480046429846755 23.850657583075634 100-101 19.88448792148566 28.382511321110478 27.83531099897415 23.897689758429713 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1212.0 1 826.5 2 535.0 3 644.5 4 596.5 5 632.5 6 1076.0 7 2061.0 8 2978.5 9 3264.5 10 2863.5 11 2420.0 12 2413.0 13 2457.0 14 2500.5 15 2453.5 16 2441.5 17 2476.0 18 2464.0 19 2575.5 20 2689.0 21 2767.5 22 3052.0 23 3414.0 24 3751.0 25 4244.0 26 4881.5 27 5629.5 28 6379.5 29 7177.5 30 8036.5 31 8910.5 32 9960.0 33 10837.0 34 12182.5 35 13394.5 36 14408.0 37 15818.5 38 16569.5 39 16460.0 40 16393.0 41 17454.5 42 18913.5 43 19966.5 44 21073.5 45 21838.5 46 21755.5 47 23006.0 48 28783.0 49 28344.5 50 22593.5 51 20161.5 52 18181.0 53 17504.0 54 16858.5 55 15532.5 56 13950.5 57 12395.0 58 11401.0 59 10594.0 60 9365.5 61 7930.5 62 6375.5 63 4626.5 64 3105.0 65 2303.5 66 1701.5 67 1307.5 68 948.5 69 607.5 70 459.0 71 360.0 72 305.5 73 281.0 74 216.0 75 151.0 76 105.0 77 62.5 78 43.0 79 32.5 80 17.5 81 13.0 82 10.0 83 6.0 84 5.5 85 2.0 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06135253582492798 2 0.0 3 0.0 4 0.0 5 0.0019172667445289994 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.5977222871074997E-4 12-13 0.004553508518256374 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.189750291983748E-4 24-25 5.592028004876248E-4 26-27 3.1954445742149993E-4 28-29 0.0032753306885703742 30-31 3.1954445742149993E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.1954445742149993E-4 40-41 7.988611435537498E-5 42-43 0.010465080980554122 44-45 7.988611435537498E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5977222871074997E-4 84-85 2.3965834306612492E-4 86-87 0.0 88-89 0.0 90-91 0.008867358693446622 92-93 0.0021569250875951244 94-95 0.0 96-97 0.0 98-99 7.988611435537498E-5 100-101 0.011024283781041746 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 625891.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.555218288837224 #Duplication Level Percentage of deduplicated Percentage of total 1 75.93559352441119 38.38940506519022 2 13.016554856328716 13.161095442606449 3 4.5089965539787835 6.8385991515003655 4 2.1659901859325403 4.380084266451948 5 1.1333899752231935 2.8649388801894182 6 0.6985546812213217 2.118935063749981 7 0.47010425420216045 1.6636356232790874 8 0.30596307987288185 1.2374424233038785 9 0.240869297684356 1.0959479931160379 >10 1.3250142650265782 12.428279383663108 >50 0.12589792619469134 4.354608575132989 >100 0.06801017741807731 6.321298207857863 >500 0.0022142848461698247 0.7279535883972884 >1k 0.0022142848461698247 2.1633910685058253 >5k 6.326528131913785E-4 2.2543852670554863 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7829 1.2508567785764615 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6268 1.0014523295589808 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4653 0.7434201801911196 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2025 0.32353876313926866 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1496 0.23901925415128192 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1493 0.23853993746514968 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1338 0.21377524201498344 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1279 0.20434868052104918 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1244 0.19875665251617292 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 996 0.15913313979590696 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 748 0.11950962707564096 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.5977222871074997E-4 0.0 14-15 0.0 0.0 0.0 1.5977222871074997E-4 0.0 16-17 0.0 0.0 0.0 3.1954445742149993E-4 0.0 18-19 0.0 0.0 0.0 5.592028004876248E-4 0.0 20-21 0.0 0.0 0.0 6.390889148429999E-4 0.0 22-23 0.0 0.0 0.0 6.390889148429999E-4 0.0 24-25 0.0 0.0 0.0 0.0011982917153306246 0.0 26-27 0.0 0.0 0.0 0.0022368112019504993 0.0 28-29 0.0 0.0 0.0 0.004553508518256374 0.0 30-31 0.0 0.0 0.0 0.008467928121669747 0.0 32-33 0.0 0.0 0.0 0.014778931155744371 0.0 34-35 0.0 0.0 0.0 0.020770389732397497 0.0 36-37 0.0 0.0 0.0 0.025962987165496866 0.0 38-39 0.0 0.0 0.0 0.03155501517037312 0.0 40-41 0.0 0.0 0.0 0.04249941283705949 0.0 42-43 0.0 0.0 0.0 0.06023413022395273 0.0 44-45 0.0 0.0 0.0 0.09091039813641671 0.0 46-47 0.0 0.0 0.0 0.12278495776421133 0.0 48-49 0.0 0.0 0.0 0.20195209709038794 0.0 50-51 0.0 0.0 0.0 0.2755272084116883 0.0 52-53 0.0 0.0 0.0 0.3594875145991874 0.0 54-55 0.0 0.0 0.0 0.4982496952344737 0.0 56-57 0.0 0.0 0.0 0.641884928845438 0.0 58-59 0.0 0.0 0.0 0.8197114194005026 0.0 60-61 0.0 0.0 0.0 1.0213439720334692 0.0 62-63 0.0 0.0 0.0 1.2783376019147104 0.0 64-65 0.0 0.0 0.0 1.482366737978338 0.0 66-67 0.0 0.0 0.0 1.7566157685603403 0.0 68-69 0.0 0.0 0.0 2.0994070852592546 0.0 70-71 0.0 0.0 0.0 2.361593312573595 0.0 72-73 0.0 0.0 0.0 2.7589468453772303 0.0 74-75 0.0 0.0 0.0 3.2472107763172824 0.0 76-77 0.0 0.0 0.0 3.627468680648867 0.0 78-79 0.0 0.0 0.0 4.004770798749303 0.0 80-81 0.0 0.0 0.0 4.536173231441257 0.0 82-83 0.0 0.0 0.0 5.149618703576182 0.0 84-85 0.0 0.0 0.0 5.786151262759809 0.0 86-87 0.0 0.0 0.0 6.6202421827442794 0.0 88-89 0.0 0.0 0.0 7.591737219419995 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACGG 40 5.387113E-4 47.498524 5 TATAGGG 60 8.28794E-5 39.5821 2 GTAAGGT 180 0.0 36.943295 4 ATATACG 60 0.0039501903 31.665682 4 AGGTAAG 185 9.094947E-12 30.809853 2 TAAGGTG 220 0.0 30.226332 5 GGTGTCG 65 0.005838392 29.234533 8 GTATCAA 9180 0.0 27.492208 1 GGTAAGG 220 7.6397555E-11 25.908283 3 GGTATCA 6555 0.0 25.66778 1 GAGTACT 5945 0.0 24.372353 12-13 AGTACTT 6045 0.0 24.008465 12-13 GGTACTC 100 0.0016531065 23.74926 3 GTACATG 4820 0.0 23.665842 1 ATGGGAG 1160 0.0 23.33979 5 GTACTTT 6360 0.0 22.81572 14-15 AAGGGTA 125 2.2547836E-4 22.799292 4 TATCAAC 11145 0.0 22.587902 2 ATCAACG 11235 0.0 22.40696 3 TACATGG 4935 0.0 22.3296 2 >>END_MODULE