FastQCFastQC Report
Thu 26 May 2016
SRR937415_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937415_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384558
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138090.9973580016149558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100890.7286801997460561No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71340.5152546877776156No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25350.183090921434855No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23470.16951258091029772No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21230.15333413262571882No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20340.14690608844122097No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20280.14647273714788403No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18010.13007761321663663No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16430.11866602915876404No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14730.10638774251421754No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA163700.031.1804891
GGTATCA110450.028.1840171
GTACATG113700.026.5006241
TACATGG113900.026.2359622
TATCAAC198450.025.5436742
ATCAACG198350.025.4837743
TCAACGC199500.025.3387094
GAGTACT99950.025.19111612-13
CAACGCA200700.025.11715
ACATGGG115050.024.7340093
AACGCAG204400.024.660656
AGTACTT102050.023.81151212-13
GTACTTT107650.023.18897814-15
ACGCAGA222250.022.4457077
CGCAGAG222450.022.3828128
CATGGGA80500.022.1320254
ATGGGAT31350.021.8236055
GCAGAGT224600.021.5551099
TACTTTT115900.020.73910314-15
CGGGTCG2751.2569217E-920.7309236
Produced by FastQC (version 0.11.5)