##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937415_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1384558 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89812488895373 33.0 31.0 34.0 30.0 34.0 2 31.978827900311867 33.0 31.0 34.0 30.0 34.0 3 31.99220328798071 33.0 31.0 34.0 30.0 34.0 4 35.63557467437261 37.0 35.0 37.0 33.0 37.0 5 35.65801721560238 37.0 35.0 37.0 35.0 37.0 6 35.727772328786514 37.0 35.0 37.0 35.0 37.0 7 35.67521115041768 37.0 35.0 37.0 33.0 37.0 8 35.66736749200828 37.0 35.0 37.0 33.0 37.0 9 37.27482055645195 39.0 37.0 39.0 34.0 39.0 10-11 37.16926448729486 39.0 37.0 39.0 34.0 39.0 12-13 37.03553047254069 39.0 37.0 39.0 33.0 39.0 14-15 38.094680757324724 40.0 38.0 41.0 33.0 41.0 16-17 38.055035975379866 40.0 38.0 41.0 33.0 41.0 18-19 38.081162002602994 40.0 38.0 41.0 33.5 41.0 20-21 38.00400127694181 40.0 38.0 41.0 33.0 41.0 22-23 38.064757850519804 40.0 38.0 41.0 33.0 41.0 24-25 38.01958747845883 40.0 38.0 41.0 33.0 41.0 26-27 37.92062484922987 40.0 38.0 41.0 33.0 41.0 28-29 37.781476110065455 40.0 37.5 41.0 33.0 41.0 30-31 37.45939931732726 39.5 37.0 41.0 32.5 41.0 32-33 37.14414925196344 39.0 36.0 41.0 32.0 41.0 34-35 37.083334898213 39.0 36.0 41.0 32.0 41.0 36-37 36.913287489581506 39.0 35.5 41.0 31.0 41.0 38-39 36.74437690584287 39.0 35.0 41.0 31.0 41.0 40-41 36.466096039313626 39.0 35.0 41.0 30.5 41.0 42-43 36.11515335580019 39.0 35.0 41.0 30.0 41.0 44-45 35.71834007676096 39.0 35.0 41.0 28.5 41.0 46-47 35.096771677315076 39.0 35.0 40.0 25.0 41.0 48-49 34.59190911467775 38.5 34.5 40.0 23.0 41.0 50-51 33.58383361332642 37.5 33.5 39.5 20.5 40.5 52-53 33.66593309922733 38.0 33.5 39.5 20.0 40.5 54-55 33.961672967112975 38.0 34.0 40.0 18.5 41.0 56-57 33.71811437296235 38.0 34.0 40.0 16.5 41.0 58-59 33.46232841094414 38.0 33.5 40.0 13.5 41.0 60-61 33.12898087331842 37.5 33.0 40.0 9.5 41.0 62-63 32.753845992728365 37.0 32.5 40.0 7.5 41.0 64-65 32.326663093926 36.5 32.0 39.5 4.5 41.0 66-67 31.896257867131602 36.0 31.0 39.0 2.0 40.5 68-69 31.41470202042818 35.5 31.0 39.0 2.0 40.0 70-71 30.950827989871136 35.0 30.5 38.0 2.0 40.0 72-73 30.4734037866236 35.0 30.0 37.5 2.0 39.5 74-75 29.93934201384124 34.5 29.0 37.0 2.0 39.0 76-77 29.42201157336854 34.0 29.0 36.0 2.0 39.0 78-79 28.890423875345057 34.0 28.5 36.0 2.0 38.5 80-81 28.313448046235692 34.0 27.5 35.5 2.0 37.0 82-83 27.770122306180024 34.0 26.0 35.0 2.0 37.0 84-85 27.216384940175853 33.0 26.0 35.0 2.0 36.0 86-87 26.64548722408162 33.0 25.0 35.0 2.0 36.0 88-89 26.09555287680256 32.5 23.5 35.0 2.0 35.5 90-91 25.54142730026478 32.0 20.5 35.0 2.0 35.0 92-93 24.898231421146676 32.0 18.5 34.0 2.0 35.0 94-95 24.145358663197932 31.0 8.5 34.0 2.0 35.0 96-97 23.316157575197284 31.0 2.0 34.0 2.0 35.0 98-99 22.179009835629856 30.5 2.0 34.0 2.0 35.0 100-101 19.16720967991229 25.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23054719998709672 1101 2 0.30617650382675876 1101 3 0.21139488192081402 1101 4 0.12487215615571046 1101 5 0.18514861092800317 1101 6 0.23156984379944134 1101 7 0.22620765909901763 1101 8 0.23367538414051126 1101 9 0.24055328449680502 1101 10-11 0.3997586653070897 1101 12-13 0.4199402262680252 1101 14-15 0.5342946608661876 1101 16-17 0.4337316071386468 1101 18-19 0.3909556348315135 1101 20-21 0.5280371543157045 1101 22-23 0.5626123692775948 1101 24-25 0.507869219665416 1101 26-27 0.46861066578303223 1101 28-29 0.43191283023586635 1101 30-31 0.5244189349771844 1101 32-33 0.5130988726613381 1101 34-35 0.6084826772261422 1101 36-37 0.516341054942707 1101 38-39 0.5746553339335776 1101 40-41 0.4861549159773233 1101 42-43 0.6112079009668889 1101 44-45 0.5769492874869542 1101 46-47 0.7010935764281925 1101 48-49 0.9019627318391699 1101 50-51 0.796001686510408 1101 52-53 0.8338385296348818 1101 54-55 0.9199775590094745 1101 56-57 1.0543100010187842 1101 58-59 1.0277745334700228 1101 60-61 1.0225010377299668 1101 62-63 1.08930204483773 1101 64-65 0.9920693885942669 1101 66-67 1.20781665803813 1101 68-69 1.0763240300428834 1101 70-71 1.1326591562694013 1101 72-73 1.176359798296044 1101 74-75 1.1837427982221982 1101 76-77 1.0897515523971926 1101 78-79 1.3544334952021835 1101 80-81 1.460218628002817 1101 82-83 1.3314202732849942 1101 84-85 1.5382599216865493 1101 86-87 1.5012052920304662 1101 88-89 1.4291791092808879 1101 90-91 1.2516167680513988 1101 92-93 1.507555509017191 1101 94-95 1.692213366087156 1101 96-97 1.8832804905309963 1101 98-99 2.279055980119118 1101 100-101 2.2751410709094344 1102 1 0.18757886895661713 1102 2 0.15487823065152995 1102 3 0.11707567485957426 1102 4 0.23937208990923153 1102 5 0.17819391405294738 1102 6 0.14531220179539162 1102 7 0.07001937084483245 1102 8 0.12235173225626994 1102 9 0.14532042813808488 1102 10-11 0.20037530957614536 1102 12-13 0.25908530762500703 1102 14-15 0.5056621852669636 1102 16-17 0.5485144685447096 1102 18-19 0.4226687686683164 1102 20-21 0.3319130857095942 1102 22-23 0.43130199823424675 1102 24-25 0.3218434008905362 1102 26-27 0.4836190412301917 1102 28-29 0.3548581248433962 1102 30-31 0.3612337571527249 1102 32-33 0.3917018133738921 1102 34-35 0.6429832892173835 1102 36-37 0.7275941622178337 1102 38-39 0.6931046222571595 1102 40-41 0.7338370780098913 1102 42-43 0.711417129416283 1102 44-45 0.732232601577266 1102 46-47 0.7143351820534676 1102 48-49 0.6680702861455075 1102 50-51 0.5270449423038173 1102 52-53 0.4077177418065716 1102 54-55 0.5629743208186966 1102 56-57 0.6647677168403376 1102 58-59 0.779526121808118 1102 60-61 0.759124178885429 1102 62-63 0.7682367305455315 1102 64-65 0.6195435761285708 1102 66-67 0.7528818082914448 1102 68-69 0.7814651113746223 1102 70-71 0.8766149540977786 1102 72-73 0.9717532234911594 1102 74-75 1.0275328221340239 1102 76-77 1.239205176710275 1102 78-79 1.251455716162571 1102 80-81 1.2044265961918903 1102 82-83 1.1455407488084965 1102 84-85 1.1108772962441193 1102 86-87 1.2644033248256257 1102 88-89 1.203819446128442 1102 90-91 1.2224535320057441 1102 92-93 1.4305913010411153 1102 94-95 1.449890662158424 1102 96-97 1.3806943149084354 1102 98-99 1.4420704155414263 1102 100-101 1.2733121950174713 1103 1 0.22610800606770098 1103 2 0.13598698487485805 1103 3 0.14617836026865305 1103 4 0.07238940788087689 1103 5 0.05205905197210825 1103 6 0.036415328347899845 1103 7 0.0718284711802255 1103 8 -0.05819755315322084 1103 9 -0.04303132396404408 1103 10-11 0.07000443988454919 1103 12-13 0.10413677092249429 1103 14-15 0.1672785842585185 1103 16-17 0.1475173607139979 1103 18-19 0.08954549381741117 1103 20-21 -0.13633365776549766 1103 22-23 -0.10437557951728849 1103 24-25 -0.06749144140069063 1103 26-27 -0.09424937022057378 1103 28-29 0.012213985351372969 1103 30-31 -0.04192144906121342 1103 32-33 0.01906321909597608 1103 34-35 0.2076188408425068 1103 36-37 0.0869712429615177 1103 38-39 0.13980872427260493 1103 40-41 0.1889426947551982 1103 42-43 0.004878518028597512 1103 44-45 0.11441803436164832 1103 46-47 0.1498690789784476 1103 48-49 0.06017917848383547 1103 50-51 0.035038090150763423 1103 52-53 -0.24784901411948823 1103 54-55 0.0909265289064578 1103 56-57 -0.024069086890001756 1103 58-59 0.06350560657733695 1103 60-61 -0.017647352109015912 1103 62-63 0.24297178681192833 1103 64-65 0.13947975463086948 1103 66-67 0.29914641601392944 1103 68-69 0.26453097573745055 1103 70-71 0.27367825817959357 1103 72-73 0.2135247647926981 1103 74-75 0.23083500744312246 1103 76-77 0.29652722996494774 1103 78-79 0.3046510169543595 1103 80-81 0.3550774888889059 1103 82-83 0.5629840546631293 1103 84-85 0.5237590966040422 1103 86-87 0.537131384639725 1103 88-89 0.6991754839183528 1103 90-91 0.5340331346880589 1103 92-93 0.6973694904153334 1103 94-95 0.667427762526799 1103 96-97 0.7875977972616717 1103 98-99 1.051195694090513 1103 100-101 1.1556316199643426 1104 1 0.09497926932422729 1104 2 0.14303286125273118 1104 3 0.12865975488189108 1104 4 0.10259011330404633 1104 5 0.11802506991205775 1104 6 0.11823486654041204 1104 7 0.1427658723344436 1104 8 0.10878840403201195 1104 9 0.04986132877320415 1104 10-11 0.14517736404857828 1104 12-13 0.15127539075192686 1104 14-15 0.26327581508467546 1104 16-17 0.3212454206809383 1104 18-19 0.33966515316128465 1104 20-21 0.23661079762036508 1104 22-23 0.07971718780646597 1104 24-25 0.2493133855557872 1104 26-27 0.28141890185076335 1104 28-29 0.21741991281572837 1104 30-31 0.2780458460408042 1104 32-33 0.2974923097446478 1104 34-35 0.41771080844794284 1104 36-37 0.5285637566432584 1104 38-39 0.5259831464164577 1104 40-41 0.41020326565858056 1104 42-43 0.3253029655366433 1104 44-45 0.3277255857316064 1104 46-47 0.5018412201347004 1104 48-49 0.6290259727473497 1104 50-51 0.6133090275519208 1104 52-53 0.5984928714253002 1104 54-55 0.7440152916706282 1104 56-57 0.6649085269654122 1104 58-59 0.7229336289455759 1104 60-61 0.6118718023380652 1104 62-63 0.696261672762553 1104 64-65 0.7715418939370267 1104 66-67 0.7725331036881222 1104 68-69 0.7509620239055579 1104 70-71 0.9558869130414642 1104 72-73 1.0495777371785415 1104 74-75 1.0033927333784654 1104 76-77 1.0882674528118237 1104 78-79 0.9447055649046341 1104 80-81 1.212825506005263 1104 82-83 1.3365444125072337 1104 84-85 1.3270069078227813 1104 86-87 1.3534551870018774 1104 88-89 1.3567910103896246 1104 90-91 1.5427816948967035 1104 92-93 1.6876612682368766 1104 94-95 1.6390843133536706 1104 96-97 1.7383655865179897 1104 98-99 1.8367965869103564 1104 100-101 1.889469253275589 1105 1 0.10783542791596545 1105 2 0.1480231586948655 1105 3 0.14010992267399658 1105 4 0.0896254699853003 1105 5 0.10341124321030293 1105 6 0.14932005147813499 1105 7 0.18552897114678757 1105 8 0.1620375216057468 1105 9 0.26509323104211546 1105 10-11 0.23873017419814602 1105 12-13 0.35341494665424733 1105 14-15 0.3098146853060513 1105 16-17 0.36295869626591326 1105 18-19 0.5162671528724729 1105 20-21 0.4365768871626514 1105 22-23 0.518181920571223 1105 24-25 0.3241326435346181 1105 26-27 0.39573576059287063 1105 28-29 0.21105218263951997 1105 30-31 0.38770540516174634 1105 32-33 0.5045968380120982 1105 34-35 0.5590062382234819 1105 36-37 0.4220349877150156 1105 38-39 0.4285748178068758 1105 40-41 0.2836967827980885 1105 42-43 0.17188544518138826 1105 44-45 0.24843169198485526 1105 46-47 0.2822925597055459 1105 48-49 0.5175972504459807 1105 50-51 0.35609156021767774 1105 52-53 0.16495323750018542 1105 54-55 0.5615380537061441 1105 56-57 0.45065801633283087 1105 58-59 0.3583011945985959 1105 60-61 0.5100339201898407 1105 62-63 0.3580777789367673 1105 64-65 0.3418240117025846 1105 66-67 0.47992051845396944 1105 68-69 0.40882777728888087 1105 70-71 0.32540906296448213 1105 72-73 0.4264102130514651 1105 74-75 0.4869341274862329 1105 76-77 0.5451036430293748 1105 78-79 0.5717602189353421 1105 80-81 0.6001553160995172 1105 82-83 0.8239795821062224 1105 84-85 0.5809570810031985 1105 86-87 0.47371194376834325 1105 88-89 0.6183476871935056 1105 90-91 0.6858559868183249 1105 92-93 0.9903904134849917 1105 94-95 0.9998235110408658 1105 96-97 0.8174211371641924 1105 98-99 0.9821582182332769 1105 100-101 0.8977753396998409 1106 1 0.0709271556127149 1106 2 -0.5169828352285393 1106 3 0.07508963289880555 1106 4 0.031116682342428703 1106 5 0.04951846554352812 1106 6 0.11480245896847663 1106 7 0.044955104652821376 1106 8 0.14131025889965798 1106 9 0.21282294643409472 1106 10-11 -0.17591497864690098 1106 12-13 -0.08145194690882818 1106 14-15 0.014536019539626466 1106 16-17 0.1320496867789771 1106 18-19 0.19652578722247682 1106 20-21 0.1970666837137358 1106 22-23 0.34066748692809057 1106 24-25 0.26498700153030796 1106 26-27 0.271093587485602 1106 28-29 0.3062504988293071 1106 30-31 0.40287838496842454 1106 32-33 0.19940193215803248 1106 34-35 0.3802611556520148 1106 36-37 0.2839904535530948 1106 38-39 0.5029976443206863 1106 40-41 0.6162200178632347 1106 42-43 0.20345990073334264 1106 44-45 0.404910985390849 1106 46-47 0.5589879099366826 1106 48-49 0.7011960330594675 1106 50-51 0.6534672379788447 1106 52-53 0.5987729168597369 1106 54-55 0.7966324752236389 1106 56-57 0.7251039228965226 1106 58-59 0.40020913964740146 1106 60-61 0.669146320032759 1106 62-63 0.8851671262880743 1106 64-65 0.8716071798011384 1106 66-67 1.1690602354305533 1106 68-69 1.0603569167130473 1106 70-71 1.0772933595741243 1106 72-73 1.33306777400691 1106 74-75 1.198339633628283 1106 76-77 1.1961332888367302 1106 78-79 1.1510156461918477 1106 80-81 1.246924195235838 1106 82-83 1.0299974467537858 1106 84-85 0.9693172434591908 1106 86-87 0.9308631422085476 1106 88-89 1.1500930901298396 1106 90-91 1.0969103833955884 1106 92-93 1.5555170609527345 1106 94-95 1.3092964074837816 1106 96-97 1.2523341047074403 1106 98-99 1.551254117562923 1106 100-101 1.0361324879702174 1107 1 0.1850804304806637 1107 2 0.15362901141355323 1107 3 0.2838464431294021 1107 4 0.26185803619280534 1107 5 0.10074232129841931 1107 6 0.184662607791644 1107 7 0.19993817159436134 1107 8 0.09549351863977762 1107 9 0.17717551870648407 1107 10-11 0.15009423524759313 1107 12-13 0.22972865553256128 1107 14-15 0.23770465872106428 1107 16-17 0.3027305834728793 1107 18-19 0.32647888734415176 1107 20-21 0.3103640538545065 1107 22-23 0.35322579586073033 1107 24-25 0.3099758181297645 1107 26-27 0.26844102748878385 1107 28-29 0.30239091684839536 1107 30-31 0.2731638008753947 1107 32-33 0.2773982530990651 1107 34-35 0.3728396290050213 1107 36-37 0.42247236334213056 1107 38-39 0.546321361794945 1107 40-41 0.4652549418449894 1107 42-43 0.29730095202715745 1107 44-45 0.4836379732199845 1107 46-47 0.6059654131590193 1107 48-49 0.4585137589749948 1107 50-51 0.6048088398678431 1107 52-53 0.6360125575467279 1107 54-55 0.6872716349973373 1107 56-57 0.8368476668715701 1107 58-59 1.0331176271173064 1107 60-61 0.9727406685793696 1107 62-63 0.8438144944430945 1107 64-65 0.9037409527681746 1107 66-67 1.0583159119766385 1107 68-69 1.2093227003814917 1107 70-71 0.9987261346099601 1107 72-73 1.1547638418546775 1107 74-75 1.2711305735393914 1107 76-77 1.324464389702154 1107 78-79 1.2951783894917384 1107 80-81 1.2169187885716504 1107 82-83 1.2414183253231776 1107 84-85 1.1951995948075158 1107 86-87 1.1229292461248122 1107 88-89 1.3359243805444265 1107 90-91 1.2580562802880664 1107 92-93 1.4749385839892746 1107 94-95 1.290927514125947 1107 96-97 1.4865656453269231 1107 98-99 1.8109023955539136 1107 100-101 1.6771223136932996 1108 1 0.31277857543826215 1108 2 0.30936715065493203 1108 3 0.2694172651360027 1108 4 0.28868574188445706 1108 5 0.24488316728852766 1108 6 0.19891034391629603 1108 7 0.2127975295256448 1108 8 0.2812867808842938 1108 9 0.40997078858654135 1108 10-11 0.30271334059467137 1108 12-13 0.4329438161514432 1108 14-15 0.4243106627877111 1108 16-17 0.4480221669786957 1108 18-19 0.49082320513610256 1108 20-21 0.5000751759199247 1108 22-23 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-1.1357282603613115 2316 50-51 -1.1816386408134676 2316 52-53 -0.9042744034938721 2316 54-55 -0.8857178443916283 2316 56-57 -1.025978644934419 2316 58-59 -0.8121915833948279 2316 60-61 -1.0258343733427893 2316 62-63 -1.0409842037238306 2316 64-65 -0.600505889503161 2316 66-67 -0.6964514982078747 2316 68-69 -0.5108331429529009 2316 70-71 -0.4578343867557848 2316 72-73 -0.6704069195149778 2316 74-75 -0.4930728263782349 2316 76-77 -0.40529383821781906 2316 78-79 -0.3422964626037235 2316 80-81 -0.5160911469620224 2316 82-83 -0.5404351480166305 2316 84-85 -0.3430572052793117 2316 86-87 -0.041963740062616495 2316 88-89 0.037321450413735135 2316 90-91 0.39038978301112337 2316 92-93 0.12943889988792634 2316 94-95 0.15294808671111682 2316 96-97 0.01259979113194376 2316 98-99 0.22879066877785803 2316 100-101 0.6315373117993452 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4514.0 3 684.0 4 670.0 5 769.0 6 914.0 7 1135.0 8 1446.0 9 1857.0 10 2607.0 11 3881.0 12 5832.0 13 8460.0 14 9388.0 15 13132.0 16 18041.0 17 25917.0 18 20521.0 19 11381.0 20 10581.0 21 10732.0 22 11634.0 23 13002.0 24 14427.0 25 16312.0 26 18766.0 27 21957.0 28 26210.0 29 30991.0 30 37753.0 31 46366.0 32 58946.0 33 75932.0 34 101186.0 35 139743.0 36 190363.0 37 241852.0 38 170181.0 39 16473.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.172898505908364 19.31702392530918 15.271367625045354 20.238709943737103 2 14.885732156870693 21.254513811466747 41.3837239270377 22.476030104624865 3 18.605361632567963 27.65770455060438 31.113496890322605 22.62343692650505 4 11.635898995164046 17.110517476037412 39.0160136680856 32.23756986071294 5 12.01508410059349 39.128096128201825 33.9101237799731 14.946695991231588 6 28.67021877144073 37.660199346320084 18.496203271873526 15.173378610365662 7 26.58098397104409 35.34925059003957 21.056216060511534 17.013549378404804 8 26.586189526343368 33.407397971738675 20.293397294300544 19.713015207617417 9 27.768629378353594 16.267776345399813 21.051709648863117 34.91188462738348 10-11 23.996939441035376 27.675039288514146 27.250158710395695 21.077862560054783 12-13 26.33249091501974 25.718465319641837 28.008919042067955 19.94012472327047 14-15 23.194409362024277 25.69320248656152 28.468292287504877 22.64409586390932 16-17 20.089981660618676 29.56693295197456 28.68546296443723 21.65762242296953 18-19 19.982798995003968 27.088833023613933 33.159405628209356 19.768962353172743 20-21 21.57239848067023 25.733910312937496 32.937193582109046 19.756497624283224 22-23 20.983488753450093 26.007384325026088 32.875515795537275 20.13361112598654 24-25 20.539759158211634 26.088998567107147 33.3433081009104 20.027934173770824 26-27 20.477201701898554 26.17796121700437 33.222046815597864 20.122790265499212 28-29 19.92239985785611 26.446760997424544 33.48588711647295 20.144952028246394 30-31 20.14123277567426 26.311263661296124 33.45053269916593 20.096970863863678 32-33 19.899321815536723 26.463561156025854 32.94228556125736 20.69483146718007 34-35 20.507281830959613 26.258596555732318 32.94321025897871 20.290911354329364 36-37 20.712969610300885 25.939437712406573 32.857885168738385 20.489707508554158 38-39 20.48953186932068 26.209601409620227 32.94961946404763 20.35124725701147 40-41 20.34977349060369 26.37458695185757 32.96855931630017 20.307080241238566 42-43 20.17207060416488 26.684404188255094 32.314276171980865 20.829249035599155 44-45 20.357139497304015 26.882432415807866 31.897741392273797 20.862686694614325 46-47 20.768680178211117 27.056440756963003 31.057578459498657 21.117300605327227 48-49 20.903602118901986 27.316906704186778 30.472822889005176 21.30666828790606 50-51 21.049368574316702 27.585483054310245 30.351248066888814 21.01390030448424 52-53 21.01349766617635 27.81151912808812 30.062855430338548 21.11212777539698 54-55 20.55688024888875 27.689922604053162 30.26034234868671 21.49285479837138 56-57 20.991251007196094 28.087953771915448 29.595120738705745 21.325674482182716 58-59 21.196441802310773 27.401653198731157 29.823240582092662 21.578664416865408 60-61 20.983042164490136 27.51256135963694 30.432281536537925 21.072114939335002 62-63 21.16577679799963 27.431906685176806 30.46093351727691 20.941382999546654 64-65 20.97864047040964 27.5398969414184 30.43491473951895 21.046547848653013 66-67 20.722078948282448 27.772603996619317 30.023669144292775 21.48164791080546 68-69 20.833799147966246 27.69967166852088 30.093578994021293 21.372950189491583 70-71 20.792973696588156 27.801874163319944 29.826115271898406 21.579036868193494 72-73 20.75365817389256 27.695206628075976 29.702060039531396 21.849075158500064 74-75 20.642953024990867 28.125775650279866 29.64121991019643 21.590051414532837 76-77 20.74211308096881 27.69989813992282 29.809939589556357 21.748049189552017 78-79 20.611331716605044 27.680356751287995 29.875894031204158 21.832417500902807 80-81 20.721716889016093 28.062811918474328 29.556185216932256 21.659285975577326 82-83 20.73882878032684 27.832350659650462 29.530103969480674 21.89871659054202 84-85 20.623789659290335 27.590287392269953 29.42503095831309 22.36089199012662 86-87 20.850302395102347 27.56341216432552 29.52282099881372 22.06346444175842 88-89 20.995673893246433 27.10077762827176 29.53818801360533 22.365360464876478 90-91 20.590248632377204 27.116812353468987 29.978436423630207 22.314502590523606 92-93 20.62705357113782 27.225979753113755 29.9232971618973 22.223669513851124 94-95 20.428195410512224 27.109093047025517 29.851352695894423 22.611358846567832 96-97 20.394381444772378 27.07561516493226 29.70622385139724 22.82377953889812 98-99 20.408367074454805 27.223422308580197 29.306615430818937 23.061595186146057 100-101 20.81363376531284 26.427088081851796 30.186648432355774 22.572629720479593 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2250.0 1 1361.5 2 535.0 3 609.5 4 544.0 5 555.0 6 916.5 7 2114.0 8 3693.0 9 4348.5 10 3890.0 11 3414.0 12 3443.5 13 3599.5 14 3810.0 15 3901.5 16 3961.0 17 4120.0 18 4265.0 19 4586.5 20 4962.0 21 5373.5 22 5961.5 23 6814.0 24 7848.5 25 9196.0 26 11055.5 27 13063.0 28 14479.5 29 16227.0 30 19234.0 31 22151.0 32 25480.0 33 29094.0 34 32674.0 35 35271.0 36 38416.5 37 42332.0 38 43974.0 39 43997.0 40 44234.0 41 45690.0 42 46840.5 43 48030.5 44 50163.0 45 51865.0 46 51229.0 47 52223.0 48 60221.5 49 58856.5 50 48176.0 51 42104.5 52 37610.5 53 35149.0 54 33620.0 55 30879.5 56 27314.0 57 24122.5 58 22160.0 59 19931.5 60 16916.0 61 14102.0 62 11392.5 63 8375.0 64 5691.5 65 4122.5 66 2970.0 67 2166.0 68 1608.5 69 1085.0 70 760.5 71 587.5 72 459.0 73 396.0 74 320.5 75 241.0 76 177.5 77 122.5 78 72.0 79 49.0 80 35.5 81 23.5 82 14.5 83 8.5 84 7.0 85 6.0 86 4.5 87 3.5 88 3.0 89 2.0 90 2.5 91 3.5 92 3.0 93 2.0 94 2.5 95 2.0 96 1.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26860557665334356 2 0.2341541488330572 3 0.23292632016860254 4 0.23386524797083258 5 0.23372079753972028 6 0.20526406261059485 7 0.20735859386172337 8 0.2081530712328411 9 0.21790347533292212 10-11 0.22469264559520077 12-13 0.21808403837181253 14-15 0.20880309817284648 16-17 0.2045418104550333 18-19 0.1922635238104868 20-21 0.19966660840499278 22-23 0.1958747845882946 24-25 0.19775264019275465 26-27 0.1942497172382811 28-29 0.20652800388282758 30-31 0.21732567360847288 32-33 0.21147543114842424 34-35 0.2094531251128519 36-37 0.20035274795277627 38-39 0.18962730344268713 40-41 0.18825502434712016 42-43 0.1980054284472012 44-45 0.19421360463050302 46-47 0.20410845916169637 48-49 0.20938089989729572 50-51 0.21967299311404795 52-53 0.22635382555299235 54-55 0.20920033685840536 56-57 0.18977175387379944 58-59 0.18128529104595112 60-61 0.1835964979437481 62-63 0.1886883756404571 64-65 0.18262145753374 66-67 0.1870633082904436 68-69 0.18565491658709854 70-71 0.1883633621704544 72-73 0.18912172693379403 74-75 0.1916134968704814 76-77 0.19937770754276815 78-79 0.1989804688572093 80-81 0.19403304159161264 82-83 0.18879671346379134 84-85 0.17749346722925297 86-87 0.1811769532226169 88-89 0.18774944783822708 90-91 0.18764111001489284 92-93 0.1925885372804895 94-95 0.18746054697600245 96-97 0.19302188857382643 98-99 0.19594700980385074 100-101 0.18402984923708504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1384558.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.14407240706741 #Duplication Level Percentage of deduplicated Percentage of total 1 72.98390433598034 33.67774566229958 2 13.953269528582435 12.877213588844702 3 5.054295829465274 6.996773781647532 4 2.4555002074219354 4.532271174673873 5 1.387768979645995 3.2018656140533426 6 0.8300792277448156 2.2981941593195625 7 0.5842081837447806 1.8870421312064345 8 0.41839984206816877 1.5445338085999318 9 0.2966292468051489 1.2318913298367593 >10 1.8047861202379927 15.600104095275768 >50 0.14670501918322834 4.675022687093602 >100 0.0777160202661942 6.326265685668414 >500 0.00423049741974553 1.3452301957081516 >1k 0.0020369061650626625 1.5622051402056345 >5k 1.566850896202048E-4 0.5157944865195074 >10k+ 3.133701792404096E-4 1.7278464590472646 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13809 0.9973580016149558 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10089 0.7286801997460561 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7134 0.5152546877776156 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2535 0.183090921434855 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2347 0.16951258091029772 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2123 0.15333413262571882 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2034 0.14690608844122097 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2028 0.14647273714788403 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1801 0.13007761321663663 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1643 0.11866602915876404 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1473 0.10638774251421754 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.222521555615583E-5 0.0 16-17 0.0 0.0 0.0 7.222521555615583E-5 0.0 18-19 0.0 0.0 0.0 7.222521555615583E-5 0.0 20-21 0.0 0.0 0.0 1.0833782333423375E-4 0.0 22-23 0.0 0.0 0.0 3.6112607778077913E-4 0.0 24-25 0.0 0.0 0.0 8.30589978895792E-4 0.0 26-27 0.0 0.0 0.0 0.001227828664454649 0.0 28-29 0.0 0.0 0.0 0.0023834321133531424 0.0 30-31 0.0 0.0 0.0 0.003936274247810493 0.0 32-33 0.0 0.0 0.0 0.0058863550678267 0.0 34-35 0.0 0.0 0.0 0.00780032328006483 0.0 36-37 0.0 0.0 0.0 0.010653219294532983 0.0 38-39 0.0 0.0 0.0 0.013470002701223062 0.0 40-41 0.0 0.0 0.0 0.018886893867934747 0.0 42-43 0.0 0.0 0.0 0.027084455833558434 0.0 44-45 0.0 0.0 0.0 0.045899124485937026 0.0 46-47 0.0 0.0 0.0 0.09085932116964403 0.0 48-49 0.0 0.0 0.0 0.12267452862213069 0.0 50-51 0.0 0.0 0.0 0.1675625000902815 0.0 52-53 0.0 0.0 0.0 0.22696773988521968 0.0 54-55 0.0 0.0 0.0 0.293378825589105 0.0 56-57 0.0 0.0 0.0 0.3692153019230686 0.0 58-59 0.0 0.0 0.0 0.4822477642684525 0.0 60-61 0.0 0.0 0.0 0.6264454071263176 0.0 62-63 0.0 0.0 0.0 0.7886271286576655 0.0 64-65 0.0 0.0 0.0 0.9304052267943994 0.0 66-67 0.0 0.0 0.0 1.1224881875659958 0.0 68-69 0.0 0.0 0.0 1.3666094161458024 0.0 70-71 0.0 0.0 0.0 1.5705373122686084 0.0 72-73 0.0 0.0 0.0 1.9013287995158024 0.0 74-75 0.0 0.0 0.0 2.186257274884837 0.0 76-77 0.0 0.0 0.0 2.4366259846102514 0.0 78-79 0.0 0.0 0.0 2.769295327461905 0.0 80-81 0.0 0.0 0.0 3.116626389071458 0.0 82-83 0.0 0.0 0.0 3.4954476446634954 0.0 84-85 0.0 0.0 0.0 4.004202063041057 0.0 86-87 0.0 0.0 0.0 4.635125433531856 0.0 88-89 0.0 3.6112607778077916E-5 0.0 5.353838553531164 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16370 0.0 31.180489 1 GGTATCA 11045 0.0 28.184017 1 GTACATG 11370 0.0 26.500624 1 TACATGG 11390 0.0 26.235962 2 TATCAAC 19845 0.0 25.543674 2 ATCAACG 19835 0.0 25.483774 3 TCAACGC 19950 0.0 25.338709 4 GAGTACT 9995 0.0 25.191116 12-13 CAACGCA 20070 0.0 25.1171 5 ACATGGG 11505 0.0 24.734009 3 AACGCAG 20440 0.0 24.66065 6 AGTACTT 10205 0.0 23.811512 12-13 GTACTTT 10765 0.0 23.188978 14-15 ACGCAGA 22225 0.0 22.445707 7 CGCAGAG 22245 0.0 22.382812 8 CATGGGA 8050 0.0 22.132025 4 ATGGGAT 3135 0.0 21.823605 5 GCAGAGT 22460 0.0 21.555109 9 TACTTTT 11590 0.0 20.739103 14-15 CGGGTCG 275 1.2569217E-9 20.730923 6 >>END_MODULE