FastQCFastQC Report
Thu 26 May 2016
SRR937415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937415_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1384558
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121610.878330846378411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96980.7004401404635991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69010.4984262125530313No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29120.21031982769952576No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28660.20699746778394262No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24510.17702400332813795No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21630.15622314124796505No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16460.1188827048054325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14800.10689331902311063No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT14430.10422098604753287No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG1252.1483356E-730.4098851
GTATCAA176450.025.9052731
GTACATG120150.025.3494781
TACATGG121700.024.393122
GGTATCA128150.023.766991
TAAGGTG5000.023.7491445
GAGTACT97300.023.53201912-13
ACATGGG122800.023.1689513
GTAAGGT3700.023.1072734
AGTACTT99050.022.9963612-13
GTACTTT102650.022.14118814-15
CCGTTGC1302.9404927E-421.924669
ATCAACG211700.021.4045183
TCAACGC213650.021.1869264
TATCAAC214250.021.149762
CAACGCA215500.021.0270825
ATGGGAG31200.020.8566195
AACGCAG218700.020.7411356
CATGGGA90050.020.6766554
TACTTTT109050.020.55863414-15
Produced by FastQC (version 0.11.5)