##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937415_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1384558 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27437781588059 33.0 31.0 34.0 31.0 34.0 2 32.455494822174295 34.0 31.0 34.0 31.0 34.0 3 32.50405689035779 34.0 31.0 34.0 31.0 34.0 4 36.13318835325064 37.0 35.0 37.0 35.0 37.0 5 36.076708234685725 37.0 35.0 37.0 35.0 37.0 6 36.13462852404883 37.0 35.0 37.0 35.0 37.0 7 36.076381776711415 37.0 35.0 37.0 35.0 37.0 8 36.06920331253728 37.0 35.0 37.0 35.0 37.0 9 37.75438587621465 39.0 37.0 39.0 35.0 39.0 10-11 37.68003940607761 39.0 37.0 39.0 35.0 39.0 12-13 37.57784361507427 39.0 37.0 39.0 35.0 39.0 14-15 38.75115379781851 40.0 38.0 41.0 35.0 41.0 16-17 38.73440838159181 40.0 38.0 41.0 34.5 41.0 18-19 38.79631839186224 40.0 38.0 41.0 35.0 41.0 20-21 38.73259950106821 40.0 38.0 41.0 34.5 41.0 22-23 38.671435938400556 40.0 38.0 41.0 34.5 41.0 24-25 38.67231383589564 40.0 38.0 41.0 34.0 41.0 26-27 38.50401391635453 40.0 38.0 41.0 34.0 41.0 28-29 38.35084951298537 40.0 38.0 41.0 34.0 41.0 30-31 38.31816254718112 40.0 38.0 41.0 34.0 41.0 32-33 38.13627778684605 40.0 37.0 41.0 34.0 41.0 34-35 37.9710698287829 40.0 37.0 41.0 33.5 41.0 36-37 37.77526582490586 40.0 37.0 41.0 33.0 41.0 38-39 37.62873747434199 40.0 37.0 41.0 33.0 41.0 40-41 37.40607182942138 40.0 36.0 41.0 33.0 41.0 42-43 37.1436982054923 40.0 36.0 41.0 32.5 41.0 44-45 36.721975894112056 40.0 36.0 41.0 31.0 41.0 46-47 36.17989748352904 39.5 35.5 41.0 30.0 41.0 48-49 35.71294088077205 39.0 35.0 41.0 28.5 41.0 50-51 35.419560610678644 39.0 35.0 41.0 27.0 41.0 52-53 35.16189245954305 39.0 35.0 40.0 26.0 41.0 54-55 34.94292185665028 39.0 35.0 40.0 26.0 41.0 56-57 34.66009838518863 38.0 34.5 40.0 24.5 41.0 58-59 34.40718084760624 38.0 34.0 40.0 24.0 41.0 60-61 34.064185104560444 38.0 34.0 40.0 23.0 41.0 62-63 33.705597743106466 37.0 34.0 40.0 22.0 41.0 64-65 33.29014349705827 37.0 33.0 39.5 20.5 41.0 66-67 32.86475864499718 36.0 33.0 39.0 19.5 40.5 68-69 32.4196848380494 36.0 32.0 39.0 17.5 40.0 70-71 31.977233528678468 35.0 32.0 38.5 14.0 40.0 72-73 31.519253075710804 35.0 31.0 37.5 9.5 39.5 74-75 31.045406548515842 35.0 31.0 37.0 7.0 39.0 76-77 29.725165359631013 33.5 29.5 35.5 6.5 38.0 78-79 30.34562654652243 34.0 30.5 36.0 2.0 38.0 80-81 30.157739870774645 34.0 31.0 36.0 2.0 37.0 82-83 29.767803515634593 34.0 30.0 35.0 2.0 37.0 84-85 29.359084632063084 34.0 30.0 35.0 2.0 36.5 86-87 29.01423342322965 34.0 29.0 35.0 2.0 36.0 88-89 28.59302246637555 34.0 29.0 35.0 2.0 36.0 90-91 28.079449181616084 34.0 28.5 35.0 2.0 35.0 92-93 27.638744277957297 33.0 27.0 35.0 2.0 35.0 94-95 27.20635069097864 33.0 26.5 35.0 2.0 35.0 96-97 26.61912754828617 33.0 25.5 35.0 2.0 35.0 98-99 25.749265108431715 32.5 24.0 34.5 2.0 35.0 100-101 22.96262345094969 29.5 8.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2066258198540325 1101 2 0.22989660333541195 1101 3 0.21546663385570497 1101 4 0.12065646823246112 1101 5 0.18251222437807257 1101 6 0.22300482171302605 1101 7 0.157609110146538 1101 8 0.1488235398045319 1101 9 0.2349662089560809 1101 10-11 0.2156944211500047 1101 12-13 0.211105830287174 1101 14-15 0.32443811346122686 1101 16-17 0.2632160873780478 1101 18-19 0.12742530322007184 1101 20-21 0.3158100006934248 1101 22-23 0.2601322255867089 1101 24-25 0.2299085240939931 1101 26-27 0.20005095841337805 1101 28-29 0.08070744209683767 1101 30-31 0.47930947586795014 1101 32-33 0.43312791904384795 1101 34-35 0.3681536280032276 1101 36-37 0.44201615091494517 1101 38-39 0.3525219932545838 1101 40-41 0.4845645400070637 1101 42-43 0.5446940849543296 1101 44-45 0.6498555037591984 1101 46-47 0.7113197660036903 1101 48-49 0.6492558752643092 1101 50-51 0.6031184023612681 1101 52-53 0.45331775869748014 1101 54-55 0.4434986442066986 1101 56-57 0.7254199950413636 1101 58-59 0.7044737843063942 1101 60-61 0.6253261948591344 1101 62-63 0.5644293497177983 1101 64-65 0.5759143869852679 1101 66-67 0.5512409105756433 1101 68-69 0.6038953779922309 1101 70-71 0.5428459165808626 1101 72-73 0.5993989051386848 1101 74-75 0.39787638900599376 1101 76-77 0.34829758563639857 1101 78-79 0.5358885563051423 1101 80-81 0.5329996018986982 1101 82-83 0.4091488615901291 1101 84-85 0.4750031170969571 1101 86-87 0.5925036190957336 1101 88-89 0.6173074960032601 1101 90-91 0.7680697398816001 1101 92-93 0.721608739152952 1101 94-95 1.0983075510911853 1101 96-97 1.192831142067444 1101 98-99 1.5634641249950647 1101 100-101 2.2770390035013612 1102 1 0.23542095420819464 1102 2 0.2429392723743362 1102 3 0.17498175697951268 1102 4 0.12805596286649745 1102 5 0.12745075711329434 1102 6 0.1681468257635217 1102 7 0.1471161417366389 1102 8 0.08728017044573733 1102 9 -0.01575816469927105 1102 10-11 0.12702095160130256 1102 12-13 0.25684566933978914 1102 14-15 0.21948476990700527 1102 16-17 0.23713331240785607 1102 18-19 0.25365835516361557 1102 20-21 0.23772768629397234 1102 22-23 0.19899303858868933 1102 24-25 0.1123756453376572 1102 26-27 0.25878516178374156 1102 28-29 0.1679331501454726 1102 30-31 0.19174969631097838 1102 32-33 0.24037335119168546 1102 34-35 0.2570298985159738 1102 36-37 0.020788138435371195 1102 38-39 0.009963246827155103 1102 40-41 0.01139357362873028 1102 42-43 0.07422916877189323 1102 44-45 -0.08551743234765041 1102 46-47 -0.19930644457176783 1102 48-49 -0.14643270163522715 1102 50-51 -0.2168359033441405 1102 52-53 -0.2798374978025606 1102 54-55 -0.17120062045742657 1102 56-57 -0.13764873883077655 1102 58-59 -0.12148351668604818 1102 60-61 0.008326048170509637 1102 62-63 0.13011923577386142 1102 64-65 0.12632657412840587 1102 66-67 -6.643853568277791E-4 1102 68-69 -0.03922737041627755 1102 70-71 -0.22563172365661543 1102 72-73 -0.033301458901469516 1102 74-75 -0.020536148297082946 1102 76-77 -0.08938163473722938 1102 78-79 0.06605179865998778 1102 80-81 0.06893831444578424 1102 82-83 -0.045476246454718705 1102 84-85 0.215854202908222 1102 86-87 0.2826057648505973 1102 88-89 0.21656423421611848 1102 90-91 0.48982330772745186 1102 92-93 0.516643920762931 1102 94-95 0.5902745745424376 1102 96-97 0.804495292945365 1102 98-99 1.1436296544305797 1102 100-101 1.9001091806002712 1103 1 0.11887704497654283 1103 2 0.12965157883296285 1103 3 0.07814665185750158 1103 4 0.022212873873030503 1103 5 0.07218744189982829 1103 6 -0.03332956172531709 1103 7 0.02455330456597693 1103 8 0.0183492423747893 1103 9 0.031404602795461756 1103 10-11 0.14522924963285533 1103 12-13 0.05688978368252151 1103 14-15 0.07835350500037919 1103 16-17 0.12131502227153845 1103 18-19 0.08980779146889262 1103 20-21 0.12806685137849882 1103 22-23 0.1363498473488889 1103 24-25 0.04282794103568932 1103 26-27 -0.01310410709317722 1103 28-29 -0.03920079872724358 1103 30-31 0.03463625854621455 1103 32-33 0.00835231648358814 1103 34-35 0.024559268567280412 1103 36-37 0.03128570287013588 1103 38-39 0.02493986004125759 1103 40-41 -0.023968813328274052 1103 42-43 0.024922732819113946 1103 44-45 -0.016348616652841486 1103 46-47 -0.021005341507063235 1103 48-49 0.14723379805658254 1103 50-51 -0.034174076886664295 1103 52-53 -0.09471454453284167 1103 54-55 -0.08990407106482223 1103 56-57 0.04453628167002677 1103 58-59 -0.12654244231627132 1103 60-61 -0.12818228098845452 1103 62-63 -0.06357282549971899 1103 64-65 -0.12969554900833202 1103 66-67 -0.10137310082549789 1103 68-69 -0.05051631317688532 1103 70-71 -0.12292941095189036 1103 72-73 -0.22771130588241562 1103 74-75 -0.2955573293658418 1103 76-77 -0.24745136425859116 1103 78-79 -0.1309762551760052 1103 80-81 -0.0537840764691353 1103 82-83 0.07092253895786271 1103 84-85 -0.06794305252000754 1103 86-87 0.0728416528991147 1103 88-89 0.21174381463512404 1103 90-91 0.25172748065567774 1103 92-93 0.3282000232813651 1103 94-95 0.3077191172478031 1103 96-97 0.5560255865118862 1103 98-99 0.6733532389064543 1103 100-101 1.1114424438453945 1104 1 0.11871232687725097 1104 2 0.12859881532876472 1104 3 0.12436917646078172 1104 4 0.01798988376208399 1104 5 0.009321227611131633 1104 6 0.05862252679275315 1104 7 0.03177344791846792 1104 8 0.012851477408418077 1104 9 0.09247324130488721 1104 10-11 -0.036281143721133446 1104 12-13 0.0489319897114342 1104 14-15 0.003434222837086054 1104 16-17 0.1309468534474192 1104 18-19 0.127458410056974 1104 20-21 0.07756464021705511 1104 22-23 -0.008147314844727305 1104 24-25 -0.022307921555999144 1104 26-27 0.05249606956102326 1104 28-29 -0.06180835785566785 1104 30-31 -0.03367639550399559 1104 32-33 0.035687762505588694 1104 34-35 -0.037128853228622916 1104 36-37 0.05688146316949627 1104 38-39 0.08669064633146206 1104 40-41 0.016582276138727536 1104 42-43 0.0023909678885516428 1104 44-45 -0.047513758483482604 1104 46-47 0.0902885907794726 1104 48-49 0.01995027404414884 1104 50-51 -0.0036260311236802067 1104 52-53 -0.0034995098286501047 1104 54-55 0.006753822572633794 1104 56-57 0.02097443181415315 1104 58-59 0.03962171478659826 1104 60-61 0.18052271247653806 1104 62-63 0.16442626735712906 1104 64-65 0.01711721545076017 1104 66-67 0.11536428719901437 1104 68-69 0.13697824098015587 1104 70-71 -0.06623654455855998 1104 72-73 0.10656139806066989 1104 74-75 0.05486729718361261 1104 76-77 0.22039160448674622 1104 78-79 0.058256791094265736 1104 80-81 0.25688858211679744 1104 82-83 0.18666863436306613 1104 84-85 0.279392512862934 1104 86-87 0.3405628734841031 1104 88-89 0.5468806917948115 1104 90-91 0.5930550130472518 1104 92-93 0.4197206219958467 1104 94-95 0.5861613376000356 1104 96-97 0.6987018975426302 1104 98-99 0.9214180996420751 1104 100-101 1.8793916438286864 1105 1 0.11385063053533884 1105 2 0.1062495246823758 1105 3 0.1461358107257169 1105 4 0.151070110013805 1105 5 0.12353373266716261 1105 6 0.00952241119545505 1105 7 0.016365024474559675 1105 8 0.10143141233194797 1105 9 0.23571942045124672 1105 10-11 0.13896353962919505 1105 12-13 0.06964089788809247 1105 14-15 0.20960082520319645 1105 16-17 0.23302253986476984 1105 18-19 0.21970311711588408 1105 20-21 0.30668079143757865 1105 22-23 0.21464457193457775 1105 24-25 0.12419818771470403 1105 26-27 0.04291870019351052 1105 28-29 0.15272755382195413 1105 30-31 0.05588798443394438 1105 32-33 0.07431820389474098 1105 34-35 0.10926859170459835 1105 36-37 0.15851027872461998 1105 38-39 0.1314942997522195 1105 40-41 0.026757927999021547 1105 42-43 -0.16434844102055735 1105 44-45 -0.0879350959205496 1105 46-47 -0.02614012792968623 1105 48-49 0.1289285706327732 1105 50-51 0.13508210975702895 1105 52-53 0.1917855715097545 1105 54-55 0.2900929393632694 1105 56-57 0.3671445258079018 1105 58-59 0.3019070712882481 1105 60-61 0.3585933870431859 1105 62-63 0.3344263248324779 1105 64-65 0.2564770909016687 1105 66-67 0.21274495579542219 1105 68-69 0.10283967796453908 1105 70-71 0.18328712846128425 1105 72-73 -0.04082785591644367 1105 74-75 0.16766267559055237 1105 76-77 0.23595808514880545 1105 78-79 0.2701971517952053 1105 80-81 0.12514573546318886 1105 82-83 0.04574877009110878 1105 84-85 0.10992015096363161 1105 86-87 0.20193825147434552 1105 88-89 0.3575635277148912 1105 90-91 0.4796164495287272 1105 92-93 0.2223457727788407 1105 94-95 0.41794876357358035 1105 96-97 0.5574558606477602 1105 98-99 0.8334084279772078 1105 100-101 1.2188227527940505 1106 1 0.10397357486074554 1106 2 0.1827926154799826 1106 3 0.18802093408341847 1106 4 0.15090476040271739 1106 5 0.11015936047896702 1106 6 0.16127115557184624 1106 7 0.10608957958076104 1106 8 0.12537007451580706 1106 9 0.13818554100489422 1106 10-11 0.01307392427373344 1106 12-13 -0.13092904155260499 1106 14-15 0.053210665571924665 1106 16-17 0.11593219352623407 1106 18-19 0.09360078637531899 1106 20-21 0.26486886973061985 1106 22-23 0.18489283748605345 1106 24-25 0.26746070778056463 1106 26-27 0.27901253954258465 1106 28-29 0.3274486985924341 1106 30-31 0.23613714562914367 1106 32-33 0.2972478028530361 1106 34-35 -0.09995593584333307 1106 36-37 0.3129235631879652 1106 38-39 0.269697724556039 1106 40-41 0.10054734493495943 1106 42-43 0.06732100443818467 1106 44-45 0.11444723027289427 1106 46-47 0.1596159503909078 1106 48-49 0.29548514775802914 1106 50-51 0.3703901078555063 1106 52-53 0.15740706213649958 1106 54-55 0.3066916289676058 1106 56-57 0.24803596689866936 1106 58-59 0.3489623841779448 1106 60-61 0.46069031291833795 1106 62-63 0.23274363418093458 1106 64-65 0.1515059237400962 1106 66-67 0.1889948955117049 1106 68-69 0.32184437664932375 1106 70-71 0.16597752164888746 1106 72-73 0.40791031986397286 1106 74-75 0.40785150140162685 1106 76-77 0.43718683944617 1106 78-79 0.3661218867191103 1106 80-81 0.2865028423961604 1106 82-83 0.45653748773598224 1106 84-85 0.6078653375675565 1106 86-87 0.5912409928331073 1106 88-89 0.8237633113824891 1106 90-91 0.6692728840399447 1106 92-93 0.5313773063275882 1106 94-95 0.3140360061621301 1106 96-97 0.5442481298644886 1106 98-99 0.5406401482332228 1106 100-101 1.200106382493594 1107 1 0.086763864889015 1107 2 0.03931653658420231 1107 3 0.08129031413128729 1107 4 0.12056064743864425 1107 5 -0.0074649205881414105 1107 6 0.06820928672541982 1107 7 0.06092001431628091 1107 8 0.025443818165911125 1107 9 4.8168539441917346E-4 1107 10-11 0.02271114523028217 1107 12-13 0.07602118464272678 1107 14-15 0.023981959163720035 1107 16-17 0.05630882771080081 1107 18-19 0.09344598789316194 1107 20-21 0.1371655376368821 1107 22-23 0.22772508106213962 1107 24-25 -0.02816151893467378 1107 26-27 0.04516867208980102 1107 28-29 0.06893252553584972 1107 30-31 0.03466037713546655 1107 32-33 -0.07817755449226382 1107 34-35 -0.059959483120501034 1107 36-37 0.01610356629295495 1107 38-39 -0.09684499801981161 1107 40-41 0.05667777100536142 1107 42-43 0.04362329744147786 1107 44-45 0.018792374023362868 1107 46-47 -0.19961874938126556 1107 48-49 -0.18484619320300055 1107 50-51 -0.23057731496187728 1107 52-53 -0.26422225732785876 1107 54-55 -0.11529805955799333 1107 56-57 -0.23571244901191335 1107 58-59 -0.20674272967792007 1107 60-61 -0.11753279005559847 1107 62-63 -0.14158594848596806 1107 64-65 0.029512083185245785 1107 66-67 -0.11746638410760113 1107 68-69 -0.011432242371306245 1107 70-71 -0.05136563995892374 1107 72-73 -0.049229004185662006 1107 74-75 -0.033652950295831374 1107 76-77 0.027108059204053347 1107 78-79 -0.030450387473681673 1107 80-81 0.11547173106039921 1107 82-83 0.16657871919175093 1107 84-85 0.21522959328923363 1107 86-87 0.41377378483810645 1107 88-89 0.49482781947533283 1107 90-91 0.5297983233426926 1107 92-93 0.4510599107691782 1107 94-95 0.5412427194518692 1107 96-97 0.778886298566853 1107 98-99 0.6451911784780506 1107 100-101 1.1661928782892161 1108 1 0.13443865255118226 1108 2 0.13541061231985907 1108 3 0.14678333557728962 1108 4 0.1531163498247352 1108 5 0.11814262867814307 1108 6 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62-63 0.5185552607266217 2316 64-65 0.6754820573599076 2316 66-67 0.5300719424130023 2316 68-69 0.7228459597592689 2316 70-71 0.9179817687736822 2316 72-73 0.7894481315803148 2316 74-75 0.5530315801580201 2316 76-77 0.3919900482370906 2316 78-79 0.48655423204993653 2316 80-81 0.5164096517644445 2316 82-83 0.5530722729186017 2316 84-85 0.3145177252033342 2316 86-87 0.3592214301173442 2316 88-89 0.23524717762964897 2316 90-91 0.5410247648144697 2316 92-93 0.5192329240397946 2316 94-95 0.5510909227827874 2316 96-97 0.7047022263277682 2316 98-99 0.7923151350960751 2316 100-101 1.0961158478946622 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 7.0 5 31.0 6 74.0 7 159.0 8 402.0 9 713.0 10 1305.0 11 2293.0 12 3817.0 13 5578.0 14 7035.0 15 10338.0 16 15099.0 17 22680.0 18 19595.0 19 10009.0 20 7680.0 21 7570.0 22 7670.0 23 8297.0 24 9329.0 25 10827.0 26 13035.0 27 15620.0 28 19155.0 29 23991.0 30 29872.0 31 37467.0 32 48462.0 33 64694.0 34 90236.0 35 131604.0 36 201549.0 37 301938.0 38 237276.0 39 19146.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.162650384768234 20.322699519491312 15.432566205426495 20.082083890313957 2 15.223053133202077 22.301918735076466 39.993846411634614 22.481181720086845 3 19.221152165528636 27.71007065070586 30.76685844868904 22.301918735076466 4 12.121774602436302 17.7529579837031 38.08175605500095 32.04351135885965 5 12.254450904626053 40.073742371167526 33.1724386984941 14.499368025712325 6 29.21264403513612 37.93022755276413 17.815866146452514 15.041262265647232 7 27.055854648198196 35.585435929733535 20.46848163818345 16.89022778388482 8 27.32019893713373 33.810284581794335 19.697260786474818 19.17225569459712 9 28.046062353473094 16.43441444850992 20.61647110485801 34.90305209315897 10-11 24.480086785819015 28.070438363723298 26.590038120468773 20.85943672998892 12-13 26.48002788025215 26.02896007020623 27.64059754747678 19.850414502064837 14-15 23.205672857330644 26.259210520613795 27.84867083935812 22.68644578269744 16-17 20.43547471467429 30.01116601832498 28.115940249523675 21.43741901747706 18-19 20.37715285311269 27.66843281393773 32.25870638860921 19.69570794434036 20-21 21.91437751276674 26.249959824622287 32.191329413179474 19.644333249431494 22-23 21.314911456759038 26.701176086517677 31.948839676732394 20.03507277999089 24-25 20.971480759362294 26.73148405283569 32.43739060125029 19.859644586551724 26-27 20.91049276827793 26.911865080081615 32.30226973149637 19.875372420144092 28-29 20.371792186405298 27.150869260604836 32.533098352485716 19.944240200504147 30-31 20.509449988155012 27.010217023268172 32.49083458042746 19.989498408149352 32-33 20.40482394130956 27.12269848922305 32.09062133794132 20.381856231526072 34-35 20.819351735355255 26.814080739123963 32.211434985027715 20.155132540493067 36-37 21.168668990392604 26.665657921156065 31.973380674554626 20.19229241389671 38-39 20.87674220825065 26.860386802281592 32.10346988117142 20.159401108296333 40-41 20.723039410411122 27.122265733902083 32.075543241958805 20.07915161372799 42-43 20.640481869229625 27.42920974659827 31.442992917001938 20.487315467170166 44-45 20.799200900215087 27.528929810091018 30.994765116376488 20.677104173317407 46-47 21.162313171423662 27.9132762946731 30.150524571740583 20.773885962162655 48-49 21.301057810507036 28.203513323385515 29.61399955798168 20.88142930812577 50-51 21.446447169421575 28.503500756198008 29.38912634934759 20.66092572503283 52-53 21.47273714788402 28.74545522831113 29.046526039356962 20.73528158444789 54-55 20.818412807553024 28.609563485242223 29.27060477061994 21.301418936584817 56-57 21.332222991019517 28.985965196113128 28.725737744464297 20.95607406840306 58-59 21.47587894476071 28.303075783029673 28.93233797356268 21.288707298646933 60-61 21.188928163356103 28.43058940109407 29.577706387164714 20.802776048385116 62-63 21.389425361739992 28.34597756107003 29.545746729281113 20.718850347908862 64-65 21.143570727986837 28.554058407087318 29.40487144633883 20.897499418587014 66-67 20.963802166467566 28.658929420074852 29.104884013526338 21.27238439993124 68-69 21.136709332509003 28.600968684591038 29.100478275377412 21.161843707522547 70-71 21.009845741384613 28.847328894853085 28.899908851777965 21.24291651198433 72-73 20.974751861154196 28.65316175023428 28.75608271956925 21.616003669042275 74-75 20.931445269898408 29.20715491875385 28.486311154895645 21.375088656452096 76-77 20.957157446636398 28.6298587708135 28.925584915908182 21.487398866641918 78-79 20.885871158882473 28.583165168956448 28.778462151820293 21.752501520340786 80-81 20.932275859877304 29.124276483903166 28.606891152266645 21.336556503952885 82-83 20.93482055058143 29.046563353049663 28.464173855200354 21.55444224116855 84-85 20.806316539857015 28.836402729973624 28.316308013654922 22.040972716514435 86-87 20.74477920029352 28.613824772960033 28.471793886568854 22.169602140177588 88-89 20.842499916941 28.291194735070686 28.5269017260382 22.33940362195011 90-91 20.575645681826717 28.242891649114295 28.768126619802548 22.413336049256444 92-93 20.60216324321084 28.41276335884479 28.705062289573842 22.28001110837053 94-95 20.230499552925917 28.318640316982023 28.747802547816704 22.703057582275356 96-97 20.238624889675982 28.163031090066287 28.509928800382507 23.088415219875223 98-99 20.12500054169068 28.43453559774483 28.12140227105624 23.319061589508248 100-101 20.11336417247188 28.366325047602263 28.5935734779556 22.926737301970256 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 925.0 1 646.0 2 455.5 3 572.5 4 554.0 5 592.0 6 1013.5 7 2042.5 8 3203.5 9 3785.0 10 3629.0 11 3396.0 12 3537.0 13 3703.0 14 3877.5 15 4045.0 16 4123.0 17 4166.0 18 4344.0 19 4548.0 20 4821.0 21 5283.0 22 5935.0 23 6793.5 24 7775.0 25 9055.5 26 10761.5 27 12682.0 28 14240.0 29 16324.0 30 19239.0 31 21674.0 32 24950.5 33 28439.0 34 32057.5 35 34900.5 36 38292.0 37 42423.5 38 43862.5 39 44003.5 40 44292.5 41 45590.5 42 46564.5 43 47815.5 44 50499.0 45 51903.0 46 50550.5 47 51464.0 48 61400.0 49 60177.0 50 48142.5 51 42213.0 52 37736.0 53 35501.0 54 34067.0 55 31315.0 56 27709.5 57 24679.5 58 22827.0 59 20757.5 60 17699.0 61 14581.5 62 11634.5 63 8461.0 64 5594.5 65 4055.0 66 2917.5 67 2158.5 68 1594.5 69 1045.5 70 751.5 71 593.5 72 494.0 73 449.5 74 375.5 75 274.0 76 198.5 77 107.5 78 60.5 79 43.0 80 25.0 81 12.5 82 6.5 83 3.0 84 3.5 85 3.0 86 1.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04391293105814274 2 0.0 3 0.0 4 0.0 5 5.778017244492467E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0047307516189282065 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.527882544465454E-4 22-23 6.139143322273246E-4 24-25 1.0833782333423375E-4 26-27 0.0023834321133531424 28-29 0.00346681034669548 30-31 4.33351293336935E-4 32-33 3.972386855588571E-4 34-35 0.0 36-37 0.0 38-39 3.6112607778077916E-5 40-41 0.0 42-43 0.008378125004514077 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.6112607778077916E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.527882544465454E-4 84-85 1.8056303889038956E-4 86-87 0.0 88-89 0.0 90-91 0.009389278022300258 92-93 0.0011917160566765712 94-95 0.0 96-97 0.0 98-99 2.889008622246233E-4 100-101 0.01227828664454649 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1384558.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.772062272276465 #Duplication Level Percentage of deduplicated Percentage of total 1 71.77288184870488 32.134199355909566 2 14.311655234387471 12.815246387466948 3 5.397760488091273 7.250066061109677 4 2.5969181739401637 4.65077528798622 5 1.469844195998482 3.2903977936894084 6 0.9152191950218306 2.4585750475380084 7 0.6061643828519546 1.899746064739862 8 0.4364859474851612 1.5633900817423385 9 0.3349414581930025 1.3496417841405772 >10 1.9104984908619238 15.95763658986312 >50 0.15731165679789344 4.861606077599268 >100 0.08269777694964363 6.693623673297173 >500 0.0058374771443490655 1.8113415169399123 >1k 0.0012972171431886812 1.1758111105554638 >5k 3.243042857971703E-4 1.205068450488401 >10k+ 1.6215214289858515E-4 0.8828747169341191 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12161 0.878330846378411 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9698 0.7004401404635991 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6901 0.4984262125530313 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2912 0.21031982769952576 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2866 0.20699746778394262 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2451 0.17702400332813795 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2163 0.15622314124796505 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1646 0.1188827048054325 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1480 0.10689331902311063 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1443 0.10422098604753287 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.4445043111231166E-4 0.0 10-11 0.0 0.0 0.0 1.4445043111231166E-4 0.0 12-13 0.0 0.0 0.0 1.4445043111231166E-4 0.0 14-15 0.0 0.0 0.0 1.4445043111231166E-4 0.0 16-17 0.0 0.0 0.0 1.4445043111231166E-4 0.0 18-19 0.0 0.0 0.0 1.4445043111231166E-4 0.0 20-21 0.0 0.0 0.0 1.8056303889038956E-4 0.0 22-23 0.0 0.0 0.0 4.3335129333693493E-4 0.0 24-25 0.0 0.0 0.0 9.028151944519479E-4 0.0 26-27 0.0 0.0 0.0 0.001300053880010805 0.0 28-29 0.0 0.0 0.0 0.00260010776002161 0.0 30-31 0.0 0.0 0.0 0.004116837286700883 0.0 32-33 0.0 0.0 0.0 0.006066918106717089 0.0 34-35 0.0 0.0 0.0 0.008053111534511374 0.0 36-37 0.0 0.0 0.0 0.011014345372313763 0.0 38-39 0.0 0.0 0.0 0.013903353994559997 0.0 40-41 0.0 0.0 0.0 0.019356357769049762 0.0 42-43 0.0 0.0 0.0 0.027481694519117292 0.0 44-45 0.0 0.0 0.0 0.04647692621038628 0.0 46-47 0.0 0.0 0.0 0.0917260237563179 0.0 48-49 0.0 0.0 0.0 0.12404680771769763 0.0 50-51 0.0 0.0 0.0 0.1699459322036347 0.0 52-53 0.0 0.0 0.0 0.23000119893857823 0.0 54-55 0.0 0.0 0.0 0.29727898722913737 0.0 56-57 0.0 0.0 0.0 0.37455996787422413 0.0 58-59 0.0 0.0 0.0 0.489000821922953 0.0 60-61 0.0 0.0 0.0 0.6338846043286016 0.0 62-63 0.0 0.0 0.0 0.7969691410544015 0.0 64-65 0.0 0.0 0.0 0.9402639687178147 0.0 66-67 0.0 0.0 0.0 1.1344414607405395 0.0 68-69 0.0 0.0 0.0 1.3806572205714747 0.0 70-71 0.0 0.0 0.0 1.5868602109842997 0.0 72-73 0.0 0.0 0.0 1.9215157472637476 0.0 74-75 0.0 0.0 0.0 2.2092971186472505 0.0 76-77 0.0 0.0 0.0 2.4622298235249085 0.0 78-79 0.0 0.0 0.0 2.8001354945043833 0.0 80-81 0.0 0.0 0.0 3.1533529111817638 0.0 82-83 0.0 0.0 0.0 3.538891111820523 0.0 84-85 0.0 0.0 0.0 4.05620421824149 0.0 86-87 0.0 0.0 0.0 4.699622551023504 0.0 88-89 0.0 0.0 0.0 5.436139186657403 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAG 125 2.1483356E-7 30.409885 1 GTATCAA 17645 0.0 25.905273 1 GTACATG 12015 0.0 25.349478 1 TACATGG 12170 0.0 24.39312 2 GGTATCA 12815 0.0 23.76699 1 TAAGGTG 500 0.0 23.749144 5 GAGTACT 9730 0.0 23.532019 12-13 ACATGGG 12280 0.0 23.168951 3 GTAAGGT 370 0.0 23.107273 4 AGTACTT 9905 0.0 22.99636 12-13 GTACTTT 10265 0.0 22.141188 14-15 CCGTTGC 130 2.9404927E-4 21.92466 9 ATCAACG 21170 0.0 21.404518 3 TCAACGC 21365 0.0 21.186926 4 TATCAAC 21425 0.0 21.14976 2 CAACGCA 21550 0.0 21.027082 5 ATGGGAG 3120 0.0 20.856619 5 AACGCAG 21870 0.0 20.741135 6 CATGGGA 9005 0.0 20.676655 4 TACTTTT 10905 0.0 20.558634 14-15 >>END_MODULE