FastQCFastQC Report
Thu 26 May 2016
SRR937414_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937414_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386410
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140431.0129038307571354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104140.7511486501107176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72100.5200481819952251No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25910.18688555333559337No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23930.17260406373295056No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21540.15536529598026558No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21360.15406697874366168No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18120.13069726848479166No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16870.12168117656393129No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16170.11663216508824951No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15350.11071760878816513No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATA405.375142E-447.5240332
GTATCAA170800.029.227221
GGTATCA118550.028.0725941
CGCACGT350.00839877327.13941482-83
GTACATG116950.026.6679521
TACATGG115900.026.3247852
CCGTTGC1109.478019E-525.909089
ACATGGG118050.024.7583893
GAGTACT103900.024.59442312-13
ATCAACG206800.024.0607643
TATCAAC208650.023.9385382
TCAACGC208050.023.8731084
CAACGCA210100.023.6627965
AGTACTT105950.023.49092912-13
AACGCAG212900.023.4041736
CATGGGG30550.023.1811754
GTACTTT113100.022.40442714-15
CATGGGA85000.021.9193444
ATGGGAG27450.021.6435285
ACGCAGA231100.021.437687
Produced by FastQC (version 0.11.5)