##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937414_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1386410 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.906118680621173 33.0 31.0 34.0 30.0 34.0 2 31.984071090081578 33.0 31.0 34.0 30.0 34.0 3 31.9979356755938 33.0 31.0 34.0 30.0 34.0 4 35.6410686593432 37.0 35.0 37.0 33.0 37.0 5 35.66444630376296 37.0 35.0 37.0 35.0 37.0 6 35.734010862587546 37.0 35.0 37.0 35.0 37.0 7 35.68979378394559 37.0 35.0 37.0 33.0 37.0 8 35.68915760849965 37.0 35.0 37.0 33.0 37.0 9 37.308456372934415 39.0 37.0 39.0 34.0 39.0 10-11 37.20168564854552 39.0 37.0 39.0 34.0 39.0 12-13 37.07879703695155 39.0 37.0 39.0 33.0 39.0 14-15 38.206953931376724 40.0 38.0 41.0 33.0 41.0 16-17 38.19809724396102 40.0 38.0 41.0 33.0 41.0 18-19 38.262889405010064 40.0 38.0 41.0 33.5 41.0 20-21 38.25058027567603 40.0 38.0 41.0 34.0 41.0 22-23 38.11345128785857 40.0 38.0 41.0 33.5 41.0 24-25 37.95723883988142 40.0 38.0 41.0 33.0 41.0 26-27 37.79574476525703 40.0 37.0 41.0 33.0 41.0 28-29 37.818358566369255 40.0 37.0 41.0 33.0 41.0 30-31 37.58782863655051 40.0 37.0 41.0 33.0 41.0 32-33 37.31009730166401 39.5 36.0 41.0 32.5 41.0 34-35 37.140983186791786 39.0 36.0 41.0 32.0 41.0 36-37 37.09590994006102 40.0 36.0 41.0 32.0 41.0 38-39 36.975105488275474 40.0 36.0 41.0 31.5 41.0 40-41 36.749067736095384 39.5 35.5 41.0 31.0 41.0 42-43 36.429539602282155 39.0 35.0 41.0 30.0 41.0 44-45 36.04834753067274 39.0 35.0 41.0 29.5 41.0 46-47 35.47960560007502 39.0 35.0 41.0 26.5 41.0 48-49 34.99922137030171 39.0 35.0 41.0 24.0 41.0 50-51 34.07730577534784 38.0 34.0 40.0 22.5 40.5 52-53 34.133325278957884 38.0 34.5 40.0 22.0 40.5 54-55 34.43960192150951 39.0 35.0 40.0 21.5 41.0 56-57 34.2657781608615 38.5 34.5 40.0 20.0 41.0 58-59 34.042701653911905 38.0 34.0 40.0 18.5 41.0 60-61 33.76460823277385 38.0 34.0 40.0 17.5 41.0 62-63 33.452680664449915 38.0 34.0 40.0 13.5 41.0 64-65 33.08539573430659 37.0 33.0 40.0 9.5 41.0 66-67 32.72019352139699 36.5 33.0 39.5 7.5 41.0 68-69 32.27112975238205 36.0 32.0 39.0 4.5 41.0 70-71 31.83790869944677 35.5 32.0 39.0 2.0 40.0 72-73 31.45733116466269 35.0 31.5 38.0 2.0 40.0 74-75 31.012992188457957 35.0 31.0 37.0 2.0 39.5 76-77 30.586752115175166 35.0 31.0 37.0 2.0 39.0 78-79 30.131374917953565 35.0 30.5 36.0 2.0 39.0 80-81 29.661784032140567 34.5 30.0 36.0 2.0 37.5 82-83 29.21689651690337 34.0 29.0 35.5 2.0 37.0 84-85 28.808675283646252 34.0 29.0 35.0 2.0 37.0 86-87 28.404326281547306 34.0 29.0 35.0 2.0 36.0 88-89 28.018208538599694 34.0 28.5 35.0 2.0 36.0 90-91 27.625585144365665 34.0 27.0 35.0 2.0 35.5 92-93 27.22832062665445 34.0 26.0 35.0 2.0 35.0 94-95 26.757781608615055 33.0 25.5 35.0 2.0 35.0 96-97 26.201997965969664 33.0 24.5 35.0 2.0 35.0 98-99 25.528773595112558 33.0 21.5 35.0 2.0 35.0 100-101 23.7288212000779 30.5 9.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09679349911513668 1101 2 0.15170959953270113 1101 3 0.12605773818968302 1101 4 0.031154998294461222 1101 5 0.077499217896694 1101 6 0.12288952542814258 1101 7 0.03905078785236071 1101 8 0.1334068534037982 1101 9 0.0860916687737614 1101 10-11 0.08258030430582863 1101 12-13 0.11550480801908947 1101 14-15 0.25398581469968917 1101 16-17 0.3004824891276243 1101 18-19 0.18377582745325327 1101 20-21 0.1662766578555619 1101 22-23 0.20839040836502676 1101 24-25 0.3017994669682267 1101 26-27 0.19050508653629805 1101 28-29 0.2067779136697041 1101 30-31 0.15352809269016632 1101 32-33 0.22293464739083646 1101 34-35 0.29819460562264055 1101 36-37 0.37095726370377946 1101 38-39 0.3460360698458089 1101 40-41 0.49081129472539686 1101 42-43 0.5640483050707275 1101 44-45 0.6287253161742399 1101 46-47 0.5678141596001396 1101 48-49 0.6725974531226484 1101 50-51 0.610160712315583 1101 52-53 0.6461889965590544 1101 54-55 0.6963766360985417 1101 56-57 0.6788905029559587 1101 58-59 0.577008122057812 1101 60-61 0.6239257783549945 1101 62-63 0.8308577919050677 1101 64-65 0.9055231819674958 1101 66-67 0.8229135856819028 1101 68-69 0.8159919299395781 1101 70-71 0.8171898963899906 1101 72-73 0.7445979296485916 1101 74-75 0.7126251648409969 1101 76-77 0.6179992868422026 1101 78-79 0.740410714662147 1101 80-81 0.897677998061706 1101 82-83 0.7615553167179208 1101 84-85 0.9659749247224312 1101 86-87 0.8905146816569491 1101 88-89 0.8573236613726145 1101 90-91 0.9185571261896612 1101 92-93 1.153959756509785 1101 94-95 1.2892623816955933 1101 96-97 1.2107960225036152 1101 98-99 1.0987311764612855 1101 100-101 1.314297431356664 1102 1 0.15797243748450995 1102 2 0.12108524356115424 1102 3 0.208745665965953 1102 4 0.11395933777007627 1102 5 0.103718657989468 1102 6 -0.004909955603359606 1102 7 0.09136884958655145 1102 8 0.06548374828881265 1102 9 0.18746275622061148 1102 10-11 0.19505824300349417 1102 12-13 0.1999297305520571 1102 14-15 0.38156000053388794 1102 16-17 0.352754279965545 1102 18-19 0.21632464875725788 1102 20-21 0.26676969996134403 1102 22-23 0.3030443204224156 1102 24-25 0.267244839033971 1102 26-27 0.33034858808269973 1102 28-29 0.43101640855824286 1102 30-31 0.3047285286648389 1102 32-33 0.33825108523689806 1102 34-35 0.5545100321191185 1102 36-37 0.5513841971845892 1102 38-39 0.577475197543933 1102 40-41 0.645227190761311 1102 42-43 0.6824199073731165 1102 44-45 0.787867270142776 1102 46-47 0.8959662542395463 1102 48-49 0.9636621391011744 1102 50-51 0.8281910116735887 1102 52-53 1.031655124426841 1102 54-55 0.9914283309720915 1102 56-57 0.818765769839878 1102 58-59 0.9929544683829832 1102 60-61 1.0979790060437935 1102 62-63 0.8364121356038154 1102 64-65 0.982639873495593 1102 66-67 1.0148766615066833 1102 68-69 0.995352429430234 1102 70-71 0.6631601420003221 1102 72-73 0.489379417177112 1102 74-75 0.6669919623147536 1102 76-77 0.5447039837788488 1102 78-79 0.7296367568039166 1102 80-81 0.8162175343391915 1102 82-83 0.7651111983731269 1102 84-85 1.0511234726609153 1102 86-87 0.9468695118611237 1102 88-89 0.9670411371556753 1102 90-91 1.0251862032615513 1102 92-93 1.1402575119306384 1102 94-95 1.0850722215321902 1102 96-97 1.0229012968716695 1102 98-99 1.15310719139136 1102 100-101 1.377971325831112 1103 1 0.2388241131687039 1103 2 0.18006176705273447 1103 3 0.2049751542663536 1103 4 0.16308460224702515 1103 5 0.11939594702441525 1103 6 0.11774705113215589 1103 7 0.15036701825037113 1103 8 0.19552678352946629 1103 9 0.224144752307474 1103 10-11 0.3334640451465205 1103 12-13 0.3456809765022797 1103 14-15 0.2799334044923256 1103 16-17 0.2442825914643123 1103 18-19 0.19388575578413025 1103 20-21 0.04963847378552799 1103 22-23 0.059428100148771534 1103 24-25 0.22254427293540857 1103 26-27 0.2500654846906656 1103 28-29 0.21283171958004488 1103 30-31 0.039956664915010265 1103 32-33 0.19171694113725835 1103 34-35 0.40584209920987746 1103 36-37 0.4034168334660748 1103 38-39 0.3999289329208864 1103 40-41 0.49932597468914963 1103 42-43 0.6587192168148164 1103 44-45 0.5160953190392163 1103 46-47 0.46395412675875747 1103 48-49 0.6385827670276143 1103 50-51 0.6897001749253491 1103 52-53 0.777188875048175 1103 54-55 0.580251807635122 1103 56-57 0.4176476807375238 1103 58-59 0.5969398084170408 1103 60-61 0.5020709872032185 1103 62-63 0.4496330068065433 1103 64-65 0.3922272160172966 1103 66-67 0.5064858718366807 1103 68-69 0.5636779279842585 1103 70-71 0.5128218588799172 1103 72-73 0.39663758455768416 1103 74-75 0.4390974809833281 1103 76-77 0.4399869596189738 1103 78-79 0.46407155883948903 1103 80-81 0.45612361748293395 1103 82-83 0.4836103221301684 1103 84-85 0.4599395126611938 1103 86-87 0.5598830145833915 1103 88-89 0.611943583137446 1103 90-91 0.4839506040652033 1103 92-93 0.5059845848617321 1103 94-95 0.5397565073690558 1103 96-97 0.7065659946007052 1103 98-99 1.0517532858678997 1103 100-101 1.1605142625089329 1104 1 0.1995766999375519 1104 2 0.09539796152171931 1104 3 0.1529088826878855 1104 4 -0.08956669105467796 1104 5 0.10051178992887344 1104 6 0.054848392027530224 1104 7 0.09275936443675192 1104 8 0.10317298656862306 1104 9 0.24989276721538545 1104 10-11 0.1549331511684784 1104 12-13 0.2758241546129412 1104 14-15 0.32678036461841486 1104 16-17 0.32920890674293446 1104 18-19 0.3736012525727972 1104 20-21 0.2179904971510993 1104 22-23 0.2528420835356542 1104 24-25 0.1977508890634354 1104 26-27 0.12316187540223922 1104 28-29 0.09925544340213577 1104 30-31 0.15700512884694717 1104 32-33 0.033602797928260486 1104 34-35 0.14969435254722185 1104 36-37 0.21095858409725565 1104 38-39 0.30397996569820407 1104 40-41 0.2478934766441867 1104 42-43 0.4105824293826359 1104 44-45 0.6219975970282192 1104 46-47 0.734384940550008 1104 48-49 0.8291300998513691 1104 50-51 0.7195270177603206 1104 52-53 0.5152405453491653 1104 54-55 0.70782161813176 1104 56-57 0.6993063011495266 1104 58-59 0.7683614310581746 1104 60-61 0.7788362273830884 1104 62-63 0.5249131918428205 1104 64-65 0.6525955238110797 1104 66-67 0.8192982225894099 1104 68-69 0.912399516543239 1104 70-71 0.7074667703270023 1104 72-73 0.7565726409697078 1104 74-75 0.6582501039299906 1104 76-77 0.7244041383450899 1104 78-79 0.7886428021099192 1104 80-81 0.7348593352430406 1104 82-83 0.8047148925572358 1104 84-85 0.964755132652968 1104 86-87 1.0212587862572633 1104 88-89 0.8041023728572085 1104 90-91 1.0183222847783497 1104 92-93 1.077548271601568 1104 94-95 1.2297755117512068 1104 96-97 1.2018849384291315 1104 98-99 1.1718542528523344 1104 100-101 1.446034974858911 1105 1 0.0319631837771297 1105 2 -0.03177777133634763 1105 3 0.04243244453444106 1105 4 0.1602416170723373 1105 5 0.11871090799146344 1105 6 0.04431247693404572 1105 7 0.17348013275911 1105 8 0.15515046582651593 1105 9 0.14214353080090802 1105 10-11 0.12213810306233341 1105 12-13 0.020689237589976983 1105 14-15 0.004503068607291993 1105 16-17 0.07781993615495253 1105 18-19 0.17630619775872702 1105 20-21 0.14846560059935854 1105 22-23 0.13375026097852327 1105 24-25 0.29709715871722864 1105 26-27 0.24351461596823043 1105 28-29 0.13677957903433224 1105 30-31 0.07192661687811608 1105 32-33 0.18131217894497098 1105 34-35 0.21973507137541048 1105 36-37 0.06307410902338972 1105 38-39 0.13223419538927317 1105 40-41 0.05035607651917218 1105 42-43 0.23724309675586852 1105 44-45 -0.060389179208115706 1105 46-47 -0.1856557266787533 1105 48-49 0.0068999313173208066 1105 50-51 -0.013601259448314806 1105 52-53 -0.035831879951203405 1105 54-55 -0.14280268334098878 1105 56-57 -0.03218455422793909 1105 58-59 -0.09145324443818481 1105 60-61 -0.04641199166267285 1105 62-63 -0.010332829067330351 1105 64-65 0.09124002061174252 1105 66-67 0.1561853804895108 1105 68-69 0.019805788763243015 1105 70-71 0.20934206551872592 1105 72-73 0.15769179815153933 1105 74-75 0.13610896160810526 1105 76-77 0.12147308446806804 1105 78-79 0.024011010464278115 1105 80-81 -0.05865833188492431 1105 82-83 0.14955919816481966 1105 84-85 0.15871445648031113 1105 86-87 0.05501272372357491 1105 88-89 -0.0401070877946168 1105 90-91 0.060622463415565875 1105 92-93 0.2518931050712965 1105 94-95 0.22984276300498507 1105 96-97 -0.019686443014816035 1105 98-99 0.32699790737834533 1105 100-101 0.704753988112742 1106 1 0.09357301524737593 1106 2 0.1571908277342473 1106 3 0.2066548750955839 1106 4 0.2607083494310203 1106 5 0.13901090153827056 1106 6 5.199737998964338E-4 1106 7 -0.007752252390432091 1106 8 0.03322506614569676 1106 9 -0.008434392185712625 1106 10-11 -0.22010045032113368 1106 12-13 -0.12816245724231834 1106 14-15 -0.0781022869598118 1106 16-17 -0.14234729570959104 1106 18-19 0.044211586751906395 1106 20-21 0.11426297190354262 1106 22-23 0.15866065953184716 1106 24-25 0.03065040804308694 1106 26-27 0.05354395985288818 1106 28-29 0.20875040481779905 1106 30-31 0.20732486204565248 1106 32-33 0.27923678974152466 1106 34-35 0.23466459747203317 1106 36-37 0.2387937302719294 1106 38-39 0.17914851951194066 1106 40-41 0.05810516056508419 1106 42-43 0.22158087988955089 1106 44-45 0.36284433271179495 1106 46-47 0.366580303435164 1106 48-49 0.5397983269384383 1106 50-51 0.5129139167853864 1106 52-53 0.3991935666421327 1106 54-55 0.6151847699186987 1106 56-57 0.512561788300097 1106 58-59 0.4784625305644781 1106 60-61 0.3318397088252354 1106 62-63 0.3996796205125932 1106 64-65 0.4350862519854175 1106 66-67 0.556334057621946 1106 68-69 0.4086443451920374 1106 70-71 0.4927576226356152 1106 72-73 0.3321147996386742 1106 74-75 0.25974572535539053 1106 76-77 0.459503107025224 1106 78-79 0.6796936600133208 1106 80-81 0.7495423666546976 1106 82-83 0.504530111682655 1106 84-85 0.4113200223198774 1106 86-87 0.5353823392077466 1106 88-89 0.4164083857208425 1106 90-91 0.3674302881614473 1106 92-93 0.6333016115349999 1106 94-95 0.7013136841361352 1106 96-97 0.9064928773410763 1106 98-99 1.2286058028917815 1106 100-101 1.3943296582837093 1107 1 0.15583772286524322 1107 2 0.08740266463753699 1107 3 0.21999882977783258 1107 4 0.12263789448324047 1107 5 0.22361584636626475 1107 6 0.1726849587529884 1107 7 0.19324143380771375 1107 8 0.1940842152576323 1107 9 0.22656531277682035 1107 10-11 0.2530989360009315 1107 12-13 0.14711163256708915 1107 14-15 0.26291205186122113 1107 16-17 0.3083035569486938 1107 18-19 0.27815151712307085 1107 20-21 0.12704399509348718 1107 22-23 0.12034355325935309 1107 24-25 0.18270091845790404 1107 26-27 0.2948256026215219 1107 28-29 0.2095552670352916 1107 30-31 0.32782229521966144 1107 32-33 0.3069478890511306 1107 34-35 0.27476195983705054 1107 36-37 0.3112407710461085 1107 38-39 0.17824216440484975 1107 40-41 0.2360298670027987 1107 42-43 0.3537452747677037 1107 44-45 0.3545431584626684 1107 46-47 0.5106577212672292 1107 48-49 0.5811230451776481 1107 50-51 0.41892737049401063 1107 52-53 0.37410003976421535 1107 54-55 0.47681632712647115 1107 56-57 0.5338977179631641 1107 58-59 0.5694196321163787 1107 60-61 0.5350267035735641 1107 62-63 0.4063946733243071 1107 64-65 0.6244944068210785 1107 66-67 0.7428490750291488 1107 68-69 0.6595247370017958 1107 70-71 0.8015290866115379 1107 72-73 0.7028748161607723 1107 74-75 0.5478327024014753 1107 76-77 0.5977021983098219 1107 78-79 0.691041391175176 1107 80-81 0.7851238855075913 1107 82-83 0.7701352335331322 1107 84-85 0.8034029868563763 1107 86-87 0.7699595505135832 1107 88-89 0.7558484046032419 1107 90-91 0.9006503271063906 1107 92-93 0.8179950676039205 1107 94-95 1.1268007909987077 1107 96-97 1.1980636568300724 1107 98-99 1.110290466844102 1107 100-101 1.4269873558112955 1108 1 0.010395947334970401 1108 2 0.004694419097429403 1108 3 0.048839959149933776 1108 4 0.04793077269647483 1108 5 0.04589425625529486 1108 6 0.06399553608126496 1108 7 0.09189573918768446 1108 8 0.033926889790571124 1108 9 -0.00963078218033786 1108 10-11 0.051045276779127846 1108 12-13 0.06337234334107222 1108 14-15 0.12726940542211196 1108 16-17 0.0930542241772443 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-2.150537192372912 2316 76-77 -2.2718109103044135 2316 78-79 -2.5505958479901523 2316 80-81 -2.6650277107114206 2316 82-83 -2.3936350536079445 2316 84-85 -2.3440519801491533 2316 86-87 -2.5606593187569686 2316 88-89 -2.4891917238076715 2316 90-91 -2.8727365107748284 2316 92-93 -2.6902089630720205 2316 94-95 -2.744231846775506 2316 96-97 -3.0635735823352555 2316 98-99 -2.9327735602648666 2316 100-101 -3.2371289274061112 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4072.0 3 795.0 4 632.0 5 718.0 6 888.0 7 1102.0 8 1296.0 9 1675.0 10 2279.0 11 3453.0 12 5328.0 13 7732.0 14 8857.0 15 12193.0 16 17083.0 17 24802.0 18 19262.0 19 9850.0 20 8967.0 21 8920.0 22 9396.0 23 10011.0 24 10782.0 25 12367.0 26 14054.0 27 16476.0 28 19524.0 29 23273.0 30 28868.0 31 36324.0 32 46180.0 33 61672.0 34 85050.0 35 123880.0 36 184323.0 37 274656.0 38 253097.0 39 36568.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.15457891569054 19.246189059678688 15.30597481364878 20.29325721098199 2 14.911891214865683 21.23896109155388 41.383287678365335 22.465860015215107 3 18.654946402642555 27.70365838843477 31.01046782712321 22.63092738179946 4 11.59030624199105 17.172634629903357 38.96037685091711 32.276682277188485 5 12.052522190386792 39.131519533470346 33.90858876805568 14.907369508087179 6 28.71661711418078 37.616118313124495 18.4594693427418 15.207795229952925 7 26.568669391436057 35.389580358536485 21.0260086367867 17.015741613240763 8 26.59405952041995 33.441465998551806 20.346525566666713 19.61794891436153 9 27.806690214123847 16.27780603986496 20.988935392868704 34.92656835314249 10-11 24.069041482421614 27.624218148071588 27.21585545839977 21.09088491110703 12-13 26.370440927457384 25.738438400892683 28.005336428423274 19.88578424322666 14-15 23.149055146874176 25.686621454971558 28.503999713828687 22.660323684325583 16-17 20.06175917534952 29.56534432944394 28.71707706961168 21.655819425594864 18-19 19.961707987089543 27.129880307651284 33.15178530458193 19.756626400677245 20-21 21.647251203207155 25.654316714544457 32.99285970031913 19.705572381929258 22-23 20.97206068902924 26.000406828798422 32.92000105500896 20.107531427163376 24-25 20.555435933970813 26.08634864023977 33.35895964866899 19.99925577712042 26-27 20.457040709455736 26.204081603161296 33.23353736893425 20.105340318448718 28-29 19.953722873430902 26.499363725641096 33.46427361025901 20.08263979066899 30-31 20.15443987540742 26.33586517406104 33.413179062086705 20.096515888444834 32-33 19.93499806692417 26.462326700655076 33.00645683459736 20.596218397823392 34-35 20.482776239814786 26.217320494664786 32.99729815575733 20.3026051097631 36-37 20.745994183210794 25.948443737919323 32.8968694112759 20.408692667593982 38-39 20.484413663935175 26.261515744582148 32.945570211583686 20.308500379898987 40-41 20.327212623174397 26.3912136347625 33.002556066367404 20.279017675695695 42-43 20.208687106634198 26.638930839878896 32.41269176018326 20.73969029330366 44-45 20.317743968685182 26.851287483791065 31.972679741726438 20.85828880579732 46-47 20.782441672411625 27.110278634101785 31.10400036998978 21.003279323496805 48-49 20.88228556231739 27.378559808982423 30.48523321098438 21.25392141771581 50-51 21.026523448763804 27.679680628798188 30.34525073371111 20.948545188726904 52-53 21.035630634879503 27.812140747350316 30.09704632658349 21.055182291186682 54-55 20.545281236743577 27.67085115008856 30.247773643318983 21.53609396984888 56-57 20.99355141706255 28.148407723848916 29.639059988078248 21.218980871010277 58-59 21.19565570815122 27.441279732249885 29.871883709050078 21.491180850548815 60-61 21.002849636859157 27.49216783378467 30.533093069833445 20.97188945952273 62-63 21.207727705782702 27.429142886252517 30.481971805644726 20.881157602320062 64-65 20.9678846931259 27.618795422204094 30.413282674453594 21.00003721021641 66-67 20.76802335137791 27.787633957036213 30.00283583410551 21.44150685748037 68-69 20.82373613162207 27.7295308666237 30.150843445918024 21.295889555836204 70-71 20.811246506331234 27.867229025703576 29.94641355211094 21.375110915854247 72-73 20.783330906747196 27.757809121483092 29.721140093260388 21.737719878509324 74-75 20.65927173588666 28.25439457888131 29.602954214872092 21.483379470359942 76-77 20.70602278791172 27.784712510084464 29.856810523322803 21.652454178681015 78-79 20.652445659635163 27.744656283915308 29.819677129863393 21.783220926586136 80-81 20.65412919642338 28.19149647218811 29.58755545343104 21.566818877957473 82-83 20.678772166742895 28.01815608248272 29.624443370262547 21.678628380511835 84-85 20.618995047924017 27.73104750785006 29.39729752771905 22.25265991650687 86-87 20.794470596777742 27.717820678762926 29.476952932663774 22.010755791795557 88-89 20.942207515407237 27.269144347549652 29.489447940524098 22.299200196519013 90-91 20.53868538511977 27.226245332880545 29.95872523288181 22.276344049117878 92-93 20.644610788934564 27.403057130915187 29.930014655992395 22.02231742415786 94-95 20.371361121597285 27.21990322213572 29.927226947523565 22.481508708743423 96-97 20.31082699835418 27.278750160752658 29.68462135344343 22.725801487449733 98-99 20.321669539984885 27.384577145342696 29.32005682089136 22.97369649378106 100-101 20.369342832598925 27.097182515513612 30.087124767088092 22.446349884799368 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2137.0 1 1306.5 2 549.5 3 638.0 4 577.5 5 586.5 6 963.0 7 2187.5 8 3823.5 9 4571.0 10 4128.5 11 3576.5 12 3643.5 13 3835.0 14 3946.5 15 4016.5 16 4041.0 17 4193.0 18 4466.5 19 4699.0 20 4991.0 21 5448.0 22 6122.5 23 7022.0 24 8010.5 25 9319.0 26 11221.0 27 13154.0 28 14534.5 29 16437.5 30 19306.5 31 22128.0 32 25570.0 33 29120.0 34 32140.5 35 34702.0 36 38226.5 37 42165.0 38 43679.0 39 43734.0 40 44199.5 41 45652.5 42 46579.0 43 48010.5 44 50587.5 45 51733.0 46 51027.5 47 52213.0 48 61237.5 49 59801.5 50 47787.5 51 41860.5 52 37520.0 53 35283.0 54 33770.5 55 30782.0 56 27291.0 57 24045.0 58 21990.0 59 20045.5 60 17028.0 61 14235.5 62 11468.5 63 8104.5 64 5448.5 65 4093.5 66 2920.5 67 2108.5 68 1524.0 69 964.5 70 718.5 71 567.5 72 443.0 73 410.0 74 329.0 75 236.5 76 175.5 77 105.0 78 62.0 79 40.0 80 27.5 81 22.0 82 13.0 83 10.5 84 10.0 85 9.5 86 7.5 87 7.0 88 7.0 89 5.0 90 3.5 91 4.5 92 5.0 93 4.0 94 4.0 95 3.0 96 3.0 97 3.5 98 3.0 99 4.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29385246788468056 2 0.25778810020123916 3 0.25288334619629116 4 0.2583651300841742 5 0.25995196226224565 6 0.19417055560764854 7 0.18479382000995376 8 0.18868877171976545 9 0.1830627303611486 10-11 0.20455709350047968 12-13 0.19568525905035308 14-15 0.18922973723501704 16-17 0.1909968912515057 18-19 0.18540691426057226 20-21 0.180646417726358 22-23 0.18288240852273135 24-25 0.17426302464638888 26-27 0.17451547522017297 28-29 0.18558723609898947 30-31 0.1947475854905836 32-33 0.18789535563072973 34-35 0.19658686824243912 36-37 0.1792399073867038 38-39 0.17956448669585476 40-41 0.1765350798104457 42-43 0.18176441312454467 44-45 0.1823775073751632 46-47 0.18666916712949272 48-49 0.19207882228200893 50-51 0.19409842687228165 52-53 0.2088487532548092 54-55 0.19092476251613882 56-57 0.1720270338500155 58-59 0.1754892131476259 60-61 0.1712336177609798 62-63 0.1789153280775528 64-65 0.1717745832762314 66-67 0.16860091892008858 68-69 0.16777143846336942 70-71 0.1787350062391356 72-73 0.1756695349860431 74-75 0.17952842232817132 76-77 0.17848255566535154 78-79 0.17657114417812914 80-81 0.17877107060681904 82-83 0.17390238096955446 84-85 0.16906975569997332 86-87 0.1672304729481178 88-89 0.16733866605116812 90-91 0.17256799936526712 92-93 0.16838453271398793 94-95 0.1667977005359165 96-97 0.16560757640236295 98-99 0.17397450970492134 100-101 0.1661124775499311 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1386410.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.17904048693219 #Duplication Level Percentage of deduplicated Percentage of total 1 72.98841400007025 33.705349251862124 2 13.865374324570915 12.805793646016605 3 5.129488689922667 7.1062459766759885 4 2.5132111102836654 4.642307104559887 5 1.3491375431898018 3.1150938614701045 6 0.8604409231629054 2.384060173641187 7 0.5736837170452641 1.8544514517288897 8 0.403066046269181 1.4890562615657754 9 0.30654231331735576 1.2740246907876023 >10 1.782004852877542 15.41148993554934 >50 0.14388711511640387 4.617433312975453 >100 0.07833845059786938 6.467255560508358 >500 0.003909093644095447 1.2738807890050188 >1k 0.0020327286949296326 1.566970128227974 >5k 1.5636374576381788E-4 0.5206144705093632 >10k+ 3.1272749152763576E-4 1.765973384916435 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14043 1.0129038307571354 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10414 0.7511486501107176 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7210 0.5200481819952251 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2591 0.18688555333559337 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2393 0.17260406373295056 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2154 0.15536529598026558 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2136 0.15406697874366168 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1812 0.13069726848479166 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1687 0.12168117656393129 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1617 0.11663216508824951 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1535 0.11071760878816513 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 7.212873536688281E-5 0.0 8 0.0 0.0 0.0 7.212873536688281E-5 0.0 9 0.0 0.0 0.0 7.212873536688281E-5 0.0 10-11 0.0 0.0 0.0 7.212873536688281E-5 0.0 12-13 0.0 0.0 0.0 7.212873536688281E-5 0.0 14-15 0.0 0.0 0.0 7.212873536688281E-5 0.0 16-17 0.0 0.0 0.0 7.212873536688281E-5 0.0 18-19 0.0 0.0 0.0 7.212873536688281E-5 0.0 20-21 0.0 0.0 0.0 1.8032183841720702E-4 0.0 22-23 0.0 0.0 0.0 4.3277241220129685E-4 0.0 24-25 0.0 0.0 0.0 4.6883677988473826E-4 0.0 26-27 0.0 0.0 0.0 6.852229859853867E-4 0.0 28-29 0.0 0.0 0.0 0.0012622528689204492 0.0 30-31 0.0 0.0 0.0 0.0028851494146753124 0.0 32-33 0.0 0.0 0.0 0.004832625269581148 0.0 34-35 0.0 0.0 0.0 0.006780101124486984 0.0 36-37 0.0 0.0 0.0 0.00948492870074509 0.0 38-39 0.0 0.0 0.0 0.012766786159938258 0.0 40-41 0.0 0.0 0.0 0.01792399073867038 0.0 42-43 0.0 0.0 0.0 0.0262187953058619 0.0 44-45 0.0 0.0 0.0 0.04205105271889268 0.0 46-47 0.0 0.0 0.0 0.0792694801682042 0.0 48-49 0.0 0.0 0.0 0.10938322718387779 0.0 50-51 0.0 0.0 0.0 0.14811635807589385 0.0 52-53 0.0 0.0 0.0 0.19907530961259656 0.0 54-55 0.0 0.0 0.0 0.25540785193413207 0.0 56-57 0.0 0.0 0.0 0.3139403206843574 0.0 58-59 0.0 7.212873536688281E-5 0.0 0.4148123570949431 0.0 60-61 0.0 7.212873536688281E-5 0.0 0.5417589313406568 0.0 62-63 0.0 7.212873536688281E-5 0.0 0.686881946898825 0.0 64-65 0.0 7.212873536688281E-5 0.0 0.8058582958864983 0.0 66-67 0.0 7.212873536688281E-5 0.0 0.9912652101470705 0.0 68-69 0.0 7.212873536688281E-5 0.0 1.2121594622081493 0.0 70-71 0.0 7.212873536688281E-5 0.0 1.3902453098289826 0.0 72-73 0.0 7.212873536688281E-5 0.0 1.7143197178323872 0.0 74-75 0.0 7.212873536688281E-5 0.0 1.9770486364062578 0.0 76-77 0.0 7.212873536688281E-5 0.0 2.2178864837962795 0.0 78-79 0.0 7.212873536688281E-5 0.0 2.5353971768812977 0.0 80-81 0.0 7.212873536688281E-5 0.0 2.8505997504345757 0.0 82-83 0.0 7.212873536688281E-5 0.0 3.2252003375624816 0.0 84-85 0.0 7.212873536688281E-5 0.0 3.7220591311372537 0.0 86-87 0.0 7.212873536688281E-5 0.0 4.346008756428473 0.0 88-89 0.0 7.212873536688281E-5 0.0 5.082046436479829 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAATA 40 5.375142E-4 47.524033 2 GTATCAA 17080 0.0 29.22722 1 GGTATCA 11855 0.0 28.072594 1 CGCACGT 35 0.008398773 27.139414 82-83 GTACATG 11695 0.0 26.667952 1 TACATGG 11590 0.0 26.324785 2 CCGTTGC 110 9.478019E-5 25.90908 9 ACATGGG 11805 0.0 24.758389 3 GAGTACT 10390 0.0 24.594423 12-13 ATCAACG 20680 0.0 24.060764 3 TATCAAC 20865 0.0 23.938538 2 TCAACGC 20805 0.0 23.873108 4 CAACGCA 21010 0.0 23.662796 5 AGTACTT 10595 0.0 23.490929 12-13 AACGCAG 21290 0.0 23.404173 6 CATGGGG 3055 0.0 23.181175 4 GTACTTT 11310 0.0 22.404427 14-15 CATGGGA 8500 0.0 21.919344 4 ATGGGAG 2745 0.0 21.643528 5 ACGCAGA 23110 0.0 21.43768 7 >>END_MODULE