FastQCFastQC Report
Thu 26 May 2016
SRR937414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937414_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386410
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126990.9159628104240449No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101110.7292936432945522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72470.5227169452037997No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30070.2168911072482166No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28810.2078028865919894No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25350.18284634415504794No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23140.16690589363896682No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18020.12997598113112283No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15530.11201592602476901No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT15050.10855374672715863No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG500.001614250138.0080538
CTAAGGG3750.026.6017992
GTATCAA174950.025.8631231
GTACATG123550.025.0435711
TACATGG124100.024.4597512
GGTATCA127150.023.6990471
ACATGGG123550.023.5305273
TACACCG1050.002195586222.6205755
AGTACTT101450.022.59596412-13
CATGGGG33600.022.3378184
GAGTACT99900.021.73383312-13
ATCAACG213750.021.1347623
TCAACGC216400.020.8759484
TATCAAC217200.020.7771852
GTACTTT104900.020.73720714-15
CATATAG2751.2587407E-920.7286723
CATGGGA87050.020.6820414
CAACGCA218550.020.670585
ATGGGGA15250.020.5587675
TACCTGG8600.020.4374282
Produced by FastQC (version 0.11.5)