FastQCFastQC Report
Thu 26 May 2016
SRR937413_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937413_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504661
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145470.9667958430503615No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107610.7151777044796137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77770.5168606084692831No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA28640.19034187767211352No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24660.16389073685036032No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23450.1558490583593248No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22950.152526050718401No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20890.1388352592377951No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT19130.1271382723417434No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16630.11052323413712457No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16440.10926049123357355No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGCG600.00393936631.6862955
GTATCAA179550.029.6784251
GGTATCA123050.025.767061
GTACATG124800.025.6723691
TACATGG123500.025.6637572
AGTACTT113200.024.48643512-13
ACATGGG126150.024.257473
ATCAACG220350.024.0009783
TATCAAC221650.023.96822
TCAACGC221050.023.9305614
CAACGCA222750.023.727385
GAGTACT112050.023.67785612-13
AACGCAG226400.023.3969086
CATATAG2452.9649527E-1023.2735183
ATGGGCG1903.2687421E-722.5139475
GTACTTT119250.022.16784114-15
CATGGGA88750.022.0100824
TACTTTT128900.021.46642114-15
ACGCAGA245800.021.4336057
CGCAGAG245800.021.3169358