##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937413_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1504661 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84007095285915 33.0 31.0 34.0 30.0 34.0 2 31.908129472352908 33.0 31.0 34.0 30.0 34.0 3 31.936641542513563 33.0 31.0 34.0 30.0 34.0 4 35.58860035582766 37.0 35.0 37.0 33.0 37.0 5 35.59236864649247 37.0 35.0 37.0 33.0 37.0 6 35.68394807867021 37.0 35.0 37.0 33.0 37.0 7 35.62917294992028 37.0 35.0 37.0 33.0 37.0 8 35.62773873982246 37.0 35.0 37.0 33.0 37.0 9 37.235838504487056 39.0 37.0 39.0 34.0 39.0 10-11 37.15770097051761 39.0 37.0 39.0 34.0 39.0 12-13 37.052601881752764 39.0 37.0 39.0 33.0 39.0 14-15 38.16174208010974 40.0 38.0 41.0 33.0 41.0 16-17 38.14028143216312 40.0 38.0 41.0 33.0 41.0 18-19 38.18446447405761 40.0 38.0 41.0 33.5 41.0 20-21 38.15908732930541 40.0 38.0 41.0 33.5 41.0 22-23 38.03322110428861 40.0 38.0 41.0 33.0 41.0 24-25 37.88835724458865 40.0 38.0 41.0 33.0 41.0 26-27 37.713429470159724 40.0 37.0 41.0 33.0 41.0 28-29 37.530311811098976 40.0 37.0 41.0 32.0 41.0 30-31 37.43273335322708 39.0 37.0 41.0 32.5 41.0 32-33 37.207000447276826 39.0 36.0 41.0 32.0 41.0 34-35 36.97645183865336 39.0 35.5 41.0 31.5 41.0 36-37 36.933949906324415 39.0 36.0 41.0 31.0 41.0 38-39 36.87517753168322 39.0 35.5 41.0 31.0 41.0 40-41 36.66469689850405 39.5 35.0 41.0 30.5 41.0 42-43 36.35364178376392 39.0 35.0 41.0 30.0 41.0 44-45 35.98396416202719 39.0 35.0 41.0 29.5 41.0 46-47 35.45448011213157 39.0 35.0 41.0 26.5 41.0 48-49 34.99671221624007 39.0 35.0 41.0 24.0 41.0 50-51 34.022829062493145 38.0 34.0 40.0 22.5 40.5 52-53 34.118460237887476 38.0 34.0 39.5 23.0 40.5 54-55 34.465306138724934 39.0 35.0 40.0 22.0 41.0 56-57 34.271877186954406 38.5 34.0 40.0 20.5 41.0 58-59 34.0475515747401 38.0 34.0 40.0 19.5 41.0 60-61 33.777018212075674 38.0 34.0 40.0 18.0 41.0 62-63 33.47369706531903 38.0 34.0 40.0 15.5 41.0 64-65 33.124387154315826 37.0 33.0 40.0 12.5 41.0 66-67 32.745641709328545 36.5 33.0 39.5 8.0 41.0 68-69 32.28165447233629 36.0 32.0 39.0 7.0 41.0 70-71 31.868056658609483 35.5 32.0 39.0 2.0 40.0 72-73 31.47236952376648 35.0 31.0 38.0 2.0 40.0 74-75 31.029390008779387 35.0 31.0 37.0 2.0 39.5 76-77 30.5998643548281 35.0 31.0 37.0 2.0 39.0 78-79 30.14888336974242 35.0 30.0 36.0 2.0 39.0 80-81 29.716662756594342 34.5 30.0 36.0 2.0 37.5 82-83 29.297282244970795 34.0 29.5 35.5 2.0 37.0 84-85 28.86533079544163 34.0 29.0 35.0 2.0 37.0 86-87 28.459539391264876 34.0 29.0 35.0 2.0 36.0 88-89 28.09227327617317 34.0 28.5 35.0 2.0 36.0 90-91 27.68659252815086 34.0 27.0 35.0 2.0 35.5 92-93 27.299456821171013 34.0 26.5 35.0 2.0 35.0 94-95 26.831247370670205 33.5 25.5 35.0 2.0 35.0 96-97 26.28408857543327 33.0 25.0 35.0 2.0 35.0 98-99 25.649372184166403 33.0 22.0 35.0 2.0 35.0 100-101 23.93964819982707 31.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15730608131695334 1101 2 0.06332117838871909 1101 3 0.21934858523949075 1101 4 0.15783348219900262 1101 5 0.12071419211365964 1101 6 0.05893927845676927 1101 7 0.1261123113151399 1101 8 -5.996752658177229E-5 1101 9 0.09134758606755611 1101 10-11 0.10962887163109514 1101 12-13 0.16736513099015582 1101 14-15 0.288332742644414 1101 16-17 0.18074511610610955 1101 18-19 0.408709907451005 1101 20-21 0.2430978568579576 1101 22-23 0.2115376471243522 1101 24-25 0.23942525825881233 1101 26-27 0.24080288826950635 1101 28-29 0.26206976268586146 1101 30-31 0.19189578608682467 1101 32-33 0.1641916005524422 1101 34-35 0.2730595254664152 1101 36-37 0.2148204751833518 1101 38-39 0.288107024973975 1101 40-41 0.1714181672338171 1101 42-43 0.2968805791682172 1101 44-45 0.2791775483406269 1101 46-47 0.3799687616384517 1101 48-49 0.49429157727799833 1101 50-51 0.5050132531284532 1101 52-53 0.613862944814052 1101 54-55 0.5069981549435028 1101 56-57 0.6563533653284779 1101 58-59 0.7006859201792963 1101 60-61 0.654081733050468 1101 62-63 0.6161679754175324 1101 64-65 0.5965545168601167 1101 66-67 0.7692791273133608 1101 68-69 0.674942590001848 1101 70-71 0.6643355269640487 1101 72-73 0.7799952100175958 1101 74-75 0.6650843089746417 1101 76-77 0.8279620817393543 1101 78-79 0.9511214453922641 1101 80-81 0.9086956000125781 1101 82-83 0.9410766478073178 1101 84-85 0.8700347982802867 1101 86-87 1.0174156752391745 1101 88-89 1.0372856789132783 1101 90-91 1.072091929220992 1101 92-93 0.8273642183604331 1101 94-95 0.8745653728708547 1101 96-97 0.8328629259460953 1101 98-99 0.9312457349518759 1101 100-101 1.0003138345400373 1102 1 0.1377033450767371 1102 2 0.025744277764534473 1102 3 0.11568978700976018 1102 4 -0.16223178042317699 1102 5 0.03723790968816587 1102 6 0.03209478611315575 1102 7 -0.007646127171845762 1102 8 0.0576156253504152 1102 9 0.28084669045282595 1102 10-11 0.09425872578809447 1102 12-13 0.2429315636493996 1102 14-15 0.24931287945642566 1102 16-17 0.29465134349744915 1102 18-19 0.1810800682625029 1102 20-21 0.3042112132427661 1102 22-23 0.3003536893554539 1102 24-25 0.3753511342897724 1102 26-27 0.2624757668600708 1102 28-29 0.32308732072618085 1102 30-31 0.24686457678241425 1102 32-33 0.3039419261171332 1102 34-35 0.34268646076760234 1102 36-37 0.4052156932493176 1102 38-39 0.5445886311928874 1102 40-41 0.5596504383032155 1102 42-43 0.5119573425812263 1102 44-45 0.5190082048087561 1102 46-47 0.7729545266031934 1102 48-49 0.9569247344820866 1102 50-51 0.8138147260279993 1102 52-53 0.7246028373402851 1102 54-55 0.86049803769032 1102 56-57 0.8043054262402549 1102 58-59 0.8248680168657714 1102 60-61 0.9880766737003981 1102 62-63 0.9323369397476142 1102 64-65 0.9630934524008055 1102 66-67 1.0242643833494043 1102 68-69 1.0164791555314139 1102 70-71 0.8585277523296533 1102 72-73 0.7043095283774505 1102 74-75 0.7157970586366034 1102 76-77 0.6592906351344574 1102 78-79 0.7866915702793769 1102 80-81 0.86715953343273 1102 82-83 0.8651255249209946 1102 84-85 0.719492190469726 1102 86-87 0.8621839380459626 1102 88-89 1.0267004607974073 1102 90-91 0.9846190596115214 1102 92-93 0.8042698320436337 1102 94-95 0.7448090100146665 1102 96-97 1.0735462874700872 1102 98-99 1.2716292276996448 1102 100-101 1.5162338229644021 1103 1 -0.12060726299775482 1103 2 -0.14007527355804683 1103 3 -0.05439637150717047 1103 4 0.004947469847387254 1103 5 -0.05993526391953452 1103 6 -0.08827723442205127 1103 7 -0.06316578472816303 1103 8 -0.02078593685971697 1103 9 0.061102481120919094 1103 10-11 -0.014003113001457734 1103 12-13 0.0916080558584298 1103 14-15 0.07790966815085199 1103 16-17 0.13460843847426673 1103 18-19 0.04674450040316458 1103 20-21 0.0051819858551027664 1103 22-23 -0.003852151210303134 1103 24-25 0.06184378471373009 1103 26-27 -0.0017659761299455567 1103 28-29 -0.13309648853496725 1103 30-31 -0.10534623662420728 1103 32-33 0.15434334761152257 1103 34-35 0.22085069793069323 1103 36-37 0.23170523066958282 1103 38-39 0.28306612016047694 1103 40-41 0.360159504013815 1103 42-43 0.5887035036801649 1103 44-45 0.29162618449591093 1103 46-47 0.3195483596034876 1103 48-49 0.32778784960315477 1103 50-51 0.33129566883667394 1103 52-53 0.3760277334711688 1103 54-55 0.54065930986674 1103 56-57 0.5911814938462072 1103 58-59 0.5083025891944857 1103 60-61 0.624146795270164 1103 62-63 0.6276410831953569 1103 64-65 0.6689099570950319 1103 66-67 0.8295926310037842 1103 68-69 0.8041663298566277 1103 70-71 0.696177142489578 1103 72-73 0.7087507027298869 1103 74-75 0.7949675792217548 1103 76-77 0.8197151983929842 1103 78-79 0.9526871822052989 1103 80-81 0.8849078029445074 1103 82-83 0.7766219670365508 1103 84-85 0.7965557916574468 1103 86-87 1.0522656693273724 1103 88-89 1.1127551003178873 1103 90-91 1.008532603069284 1103 92-93 1.0392709123023742 1103 94-95 1.1414660821251772 1103 96-97 1.0439790580400583 1103 98-99 1.0876210064493783 1103 100-101 1.4014876107564476 1104 1 0.1098789053183502 1104 2 0.06530966609149402 1104 3 0.041817914851677074 1104 4 -0.058484326975950296 1104 5 -0.016596116625215984 1104 6 0.09882241119704815 1104 7 0.05150041527467408 1104 8 0.10281519963429986 1104 9 0.17995157467154144 1104 10-11 0.18095569295791591 1104 12-13 0.12161537809753753 1104 14-15 0.16068142570738786 1104 16-17 0.24503228396470433 1104 18-19 0.17064664795917395 1104 20-21 0.2817251026280658 1104 22-23 0.4380348271929577 1104 24-25 0.34808564549062737 1104 26-27 0.37981823799913883 1104 28-29 0.3292640763087462 1104 30-31 0.21696119686652793 1104 32-33 0.1806445920054358 1104 34-35 0.25612104067078434 1104 36-37 0.1384744666944826 1104 38-39 0.12125575455127802 1104 40-41 0.047972036644829075 1104 42-43 0.0843449666325995 1104 44-45 0.09133377835399159 1104 46-47 0.22166159261699647 1104 48-49 0.2790959263680648 1104 50-51 0.08549467646702169 1104 52-53 0.17605551414947485 1104 54-55 0.2828470415066775 1104 56-57 0.3496305907485677 1104 58-59 0.164458523237613 1104 60-61 0.07862821831123057 1104 62-63 0.2810816331883359 1104 64-65 0.06565301095861287 1104 66-67 -0.027018622555814886 1104 68-69 0.13005044510970265 1104 70-71 -0.021806109177585853 1104 72-73 0.06671972888497635 1104 74-75 0.18856532531280124 1104 76-77 0.0874495519411127 1104 78-79 0.08948414089781664 1104 80-81 0.2323088914830116 1104 82-83 0.15614438430362654 1104 84-85 0.238480638154698 1104 86-87 0.23754184536534595 1104 88-89 0.128024966081135 1104 90-91 0.2981821088826031 1104 92-93 0.29479546436310855 1104 94-95 0.3972134919475465 1104 96-97 0.38745185553502637 1104 98-99 0.5436432580636854 1104 100-101 0.74811457876935 1105 1 0.22262376229728176 1105 2 0.21430974805198488 1105 3 0.3128954221598228 1105 4 0.28584810479193834 1105 5 0.21565807019007366 1105 6 0.14577894481450926 1105 7 0.15492499795915649 1105 8 0.24051429533683688 1105 9 0.2440747339754239 1105 10-11 0.2882467619959712 1105 12-13 0.38481784784045914 1105 14-15 0.4162315172299387 1105 16-17 0.4285869106346496 1105 18-19 0.4612293870789159 1105 20-21 0.5340514146789843 1105 22-23 0.5667653957255894 1105 24-25 0.5630325077696767 1105 26-27 0.33379537102101864 1105 28-29 0.3871083786883318 1105 30-31 0.4435717205940861 1105 32-33 0.302398273397678 1105 34-35 0.3027818335019745 1105 36-37 0.37633748338710404 1105 38-39 0.24215226576620097 1105 40-41 0.27975836563425815 1105 42-43 0.19197548872095638 1105 44-45 0.2902122855801821 1105 46-47 0.22786805110399655 1105 48-49 0.17277130816736985 1105 50-51 0.06451278973641905 1105 52-53 0.11468417846661083 1105 54-55 0.31611753846048174 1105 56-57 0.37413131473293504 1105 58-59 0.4443932280432321 1105 60-61 0.5832651333182 1105 62-63 0.6238165183070379 1105 64-65 0.3751003240203872 1105 66-67 0.5258773149356486 1105 68-69 0.511021950177593 1105 70-71 0.48144499045905675 1105 72-73 0.6631749374744302 1105 74-75 0.5333608338776727 1105 76-77 0.7007927205338511 1105 78-79 0.8264011968768337 1105 80-81 0.6510238875215855 1105 82-83 0.6920102969337378 1105 84-85 0.8040271780557973 1105 86-87 0.4966162381746777 1105 88-89 0.5255661512298921 1105 90-91 0.6983842781037026 1105 92-93 0.8311039636664432 1105 94-95 0.7583088939637754 1105 96-97 0.8519049058314501 1105 98-99 0.8391388458570361 1105 100-101 1.0583493955876442 1106 1 0.14016357855439665 1106 2 0.19822747701533316 1106 3 0.07351309072325662 1106 4 0.08847867986267488 1106 5 0.1867122075839731 1106 6 0.15688934949391609 1106 7 0.11072656835683858 1106 8 0.15284541400676943 1106 9 0.1149413137509967 1106 10-11 0.15770109648432395 1106 12-13 0.06295387479789127 1106 14-15 0.08064325388632199 1106 16-17 -0.01353169571687829 1106 18-19 0.13712664365138494 1106 20-21 0.16378883401331024 1106 22-23 0.35996881626791577 1106 24-25 0.15258968105383985 1106 26-27 0.3277410746799845 1106 28-29 0.3445709466382354 1106 30-31 0.295019728986496 1106 32-33 0.42676010065392944 1106 34-35 0.4557183818060082 1106 36-37 0.25829539418597136 1106 38-39 0.258704225584367 1106 40-41 0.5724658711179345 1106 42-43 0.5963282559905707 1106 44-45 0.522594585227317 1106 46-47 0.6537645129407963 1106 48-49 0.7779053006014891 1106 50-51 0.6450274605569035 1106 52-53 0.798586012716541 1106 54-55 0.9216282356026113 1106 56-57 0.8086983427394259 1106 58-59 0.6119894705329756 1106 60-61 0.6168419206787306 1106 62-63 0.821757358183703 1106 64-65 0.78656120713066 1106 66-67 0.8205064912716011 1106 68-69 0.8777312676586035 1106 70-71 0.8787571944200216 1106 72-73 0.8806321012217708 1106 74-75 0.9151574133353435 1106 76-77 0.816170855766071 1106 78-79 0.8227017023287218 1106 80-81 0.9503205464528257 1106 82-83 0.7563656073574911 1106 84-85 0.9276944063356716 1106 86-87 0.8037823697193254 1106 88-89 0.9766128181084888 1106 90-91 1.063500945300195 1106 92-93 1.135364541598225 1106 94-95 1.2807617661384896 1106 96-97 1.2730949430250789 1106 98-99 1.5881025105421607 1106 100-101 1.8491996038083904 1107 1 0.12948540963011013 1107 2 0.08701768015213673 1107 3 0.138330993441226 1107 4 0.1845738628427327 1107 5 0.20037612456369658 1107 6 0.2379498589543445 1107 7 0.12341158370735172 1107 8 0.2838362821689273 1107 9 0.28771696087559206 1107 10-11 0.2406978302093492 1107 12-13 0.309789854827514 1107 14-15 0.325510641160605 1107 16-17 0.27578425017870245 1107 18-19 0.38321397972459437 1107 20-21 0.32981663168082065 1107 22-23 0.2827214131036513 1107 24-25 0.269161857883887 1107 26-27 0.2917767388716115 1107 28-29 0.3033241712265138 1107 30-31 0.23482782307090844 1107 32-33 0.17245582703228735 1107 34-35 0.35827966124417543 1107 36-37 0.2625682801802256 1107 38-39 0.32387842691638014 1107 40-41 0.3256254772032108 1107 42-43 0.3335395143401598 1107 44-45 0.46117905227521305 1107 46-47 0.6010820968781729 1107 48-49 0.6042959196243558 1107 50-51 0.633523404686926 1107 52-53 0.6682079389359998 1107 54-55 0.7722233744438256 1107 56-57 0.7906102415927947 1107 58-59 0.9384060824347742 1107 60-61 0.7566426582879657 1107 62-63 0.8544039236906542 1107 64-65 0.8762905869307573 1107 66-67 0.9881460926487264 1107 68-69 1.0995022654908908 1107 70-71 1.2965186680691296 1107 72-73 1.2611896729964513 1107 74-75 1.23023009531002 1107 76-77 1.0600382528489867 1107 78-79 0.9886319040923119 1107 80-81 0.9758928802210107 1107 82-83 0.999640072541748 1107 84-85 1.0124743496417707 1107 86-87 1.112867861769427 1107 88-89 1.2739414367586264 1107 90-91 1.278290893412521 1107 92-93 1.1890763737528864 1107 94-95 1.1029812637402756 1107 96-97 1.4750853599900893 1107 98-99 1.473014870401311 1107 100-101 1.6305153829571992 1108 1 0.028335809842651827 1108 2 -0.1537774314019913 1108 3 -0.1362186051476897 1108 4 -0.02032457086501438 1108 5 0.03731633139177859 1108 6 0.02708708980716068 1108 7 0.005792843333125575 1108 8 -0.09522921605424273 1108 9 0.016210455798777446 1108 10-11 -0.03796356021711489 1108 12-13 0.08807849925860012 1108 14-15 0.14389105381798828 1108 16-17 0.0248654692538679 1108 18-19 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64-65 -1.706460561326697 2316 66-67 -1.5715720507311417 2316 68-69 -1.2833878775533485 2316 70-71 -1.1862335746354766 2316 72-73 -1.7227405596465317 2316 74-75 -2.0905581984833717 2316 76-77 -1.891220178954189 2316 78-79 -1.7022250844998972 2316 80-81 -1.6368753049302605 2316 82-83 -1.6470056293819262 2316 84-85 -1.91757916882338 2316 86-87 -1.8372552635338266 2316 88-89 -1.8382109394685244 2316 90-91 -1.7867331297022027 2316 92-93 -1.8216769342135954 2316 94-95 -1.92427265894068 2316 96-97 -1.6416246589438437 2316 98-99 -1.7991712147977523 2316 100-101 -2.5946809791005094 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4642.0 3 760.0 4 680.0 5 800.0 6 865.0 7 1180.0 8 1431.0 9 1993.0 10 2593.0 11 3870.0 12 5787.0 13 8284.0 14 9575.0 15 12997.0 16 18494.0 17 25790.0 18 20196.0 19 10447.0 20 9512.0 21 9628.0 22 10130.0 23 10759.0 24 12144.0 25 13549.0 26 15472.0 27 18605.0 28 21912.0 29 26412.0 30 32513.0 31 40882.0 32 51810.0 33 68363.0 34 94561.0 35 134920.0 36 198272.0 37 294573.0 38 269611.0 39 40642.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.960094275358166 19.37217303900723 15.270155157083382 20.39757752855122 2 14.873055451532382 21.27432309789743 41.26554072873674 22.58708072183345 3 18.69495857899843 27.58921241457252 31.042495175546147 22.673333830882903 4 11.611619949423433 17.227751141970888 38.87798653290642 32.28264237569926 5 12.087389314197765 39.29434261461686 33.734167516173954 14.884100555011425 6 28.82718445826128 37.492766584249566 18.472595592627343 15.207453364861815 7 26.65983842643248 35.32662008101987 20.98252072612874 17.031020766418912 8 26.692247297485377 33.41475450119435 20.284126135329174 19.6088720659911 9 27.8299596921071 16.277383296563674 20.971558063543032 34.92109894778619 10-11 24.07207059612515 27.66574395685008 27.228264289931342 21.033921157093427 12-13 26.425372363982667 25.67701482796168 27.961779621792076 19.935833186263572 14-15 23.115131849461584 25.758546834251195 28.41218309467598 22.71413822161124 16-17 20.18821597388431 29.547537136410796 28.624437776117357 21.63980911358754 18-19 20.075416192374547 27.123793822011528 33.009532897034525 19.7912570885794 20-21 21.707325193859102 25.7157887376934 32.813678844802844 19.763207223644653 22-23 21.116332402645703 26.00806921492625 32.67935491560885 20.1962434668192 24-25 20.617500634206394 26.18789687837286 33.187205077379836 20.0073974100409 26-27 20.57467008595046 26.262503504410727 33.010383710500044 20.152442699138764 28-29 20.027448863838785 26.555177576901933 33.25361265204836 20.16376090721092 30-31 20.2220773391476 26.404740562362395 33.21170702600037 20.161475072489633 32-33 19.99395301197277 26.5392514787816 32.75562842723702 20.711167082008615 34-35 20.6413063853292 26.304535797511075 32.73954098985072 20.31461682730901 36-37 20.78757423420202 26.047902098251342 32.66068498062761 20.503838686919032 38-39 20.604705111541985 26.310744838386636 32.74678131591844 20.33776873415294 40-41 20.423315934872626 26.45717934530385 32.77435867729085 20.345146042532676 42-43 20.286947121922896 26.708634081495887 32.18174294275939 20.822675853821828 44-45 20.446368845480357 26.920045643110697 31.707344685064864 20.92624082634408 46-47 20.821329750097966 27.152330007454346 30.9711923990309 21.055147843416787 48-49 20.936199484779966 27.40941280431994 30.398281667602834 21.25610604329726 50-51 21.097315465293377 27.65901194791426 30.211620796736284 21.03205179005608 52-53 21.068599847818597 27.882197606056618 29.940975592699953 21.10822695342483 54-55 20.630620760755512 27.675945816257585 30.115606147235418 21.577827275751478 56-57 21.08320749742336 28.142701916700624 29.48754823709882 21.286542348777193 58-59 21.258023003993777 27.498440503113336 29.702673680019863 21.540862812873023 60-61 20.998478679880293 27.5368262665739 30.38525684344384 21.079438210101962 62-63 21.239175795861136 27.49304574428846 30.311000707077028 20.95677775277338 64-65 21.02584809914208 27.63028243562276 30.24658356890718 21.09728589632798 66-67 20.807760391228978 27.798757716827755 29.89253454864943 21.500947343293838 68-69 20.8880507457569 27.84398775630452 29.96409780975002 21.303863688188564 70-71 20.84513222190189 27.931656221057626 29.782436595608118 21.440774961432364 72-73 20.801273450188347 27.840475126635845 29.619336679214275 21.738914743961534 74-75 20.665915175613872 28.271732595247496 29.5553707529714 21.506981476167237 76-77 20.821437739111847 27.794922230697345 29.716073876259628 21.66756615393118 78-79 20.707882518309486 27.83461633474651 29.724826659972553 21.732674486971447 80-81 20.75073228655499 28.217674945663155 29.48340966068975 21.548183107092104 82-83 20.752143945845848 28.07129106857364 29.450880850613302 21.72568413496721 84-85 20.64000266307152 27.773805362760275 29.371947870374992 22.214244103793213 86-87 20.876539276753398 27.702603958085533 29.414563003694834 22.006293761466242 88-89 20.989708143264348 27.257207196109906 29.452541403466487 22.300543257159255 90-91 20.57853974493675 27.302913239345845 29.84592203752309 22.272624978194315 92-93 20.622937295102844 27.43307206154131 29.86342242631526 22.080568217040586 94-95 20.347627836861015 27.266825405703422 29.860317637877902 22.52522911955766 96-97 20.334213525937052 27.28017822235716 29.667650724660838 22.71795752704495 98-99 20.305050336235198 27.43455490409998 29.343732948119673 22.916661811545158 100-101 20.344214862828018 27.13460749798942 30.065789824081122 22.455387815101435 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2525.0 1 1552.5 2 630.0 3 703.5 4 643.0 5 655.5 6 1102.5 7 2498.0 8 4177.0 9 4888.0 10 4368.5 11 3731.0 12 3816.5 13 3982.5 14 4097.0 15 4242.0 16 4334.0 17 4489.5 18 4666.0 19 4962.5 20 5418.5 21 5834.5 22 6421.0 23 7382.5 24 8424.5 25 9864.0 26 11965.5 27 13889.0 28 15456.0 29 17726.5 30 20808.5 31 23735.0 32 27407.0 33 31216.0 34 34878.0 35 37854.5 36 41461.0 37 45711.0 38 47452.5 39 47740.5 40 47992.0 41 49212.5 42 50422.5 43 51991.0 44 54451.0 45 55972.5 46 55370.0 47 57129.5 48 66968.0 49 64850.5 50 52286.5 51 45682.5 52 40498.5 53 38358.5 54 37017.0 55 33892.5 56 30014.5 57 26425.5 58 24179.0 59 22034.5 60 18704.5 61 15500.0 62 12549.5 63 9030.5 64 6042.0 65 4455.5 66 3255.0 67 2376.0 68 1714.5 69 1149.0 70 836.5 71 638.5 72 510.5 73 473.0 74 377.5 75 261.0 76 201.0 77 133.5 78 75.5 79 55.0 80 37.0 81 21.0 82 15.5 83 11.5 84 7.5 85 4.0 86 5.5 87 5.5 88 1.5 89 4.0 90 4.5 91 1.5 92 1.5 93 0.5 94 0.5 95 1.0 96 1.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29076316858082984 2 0.26098902011815284 3 0.2640461871478027 4 0.2635809660780734 5 0.25195043933484024 6 0.1960574508145024 7 0.20296930670762386 8 0.19964629906670006 9 0.1973866538708719 10-11 0.20639200457777535 12-13 0.20642523465418455 14-15 0.1921695318746216 16-17 0.2017065638040728 18-19 0.19017572729006732 20-21 0.194462407146859 22-23 0.19300028378485254 24-25 0.1850250654466355 26-27 0.19818417570469363 28-29 0.19668882226627793 30-31 0.20383328869426404 32-33 0.20516249175063353 34-35 0.19469501768172368 36-37 0.19343227477817262 38-39 0.18642072865582346 40-41 0.186354268503005 42-43 0.1848589150645893 44-45 0.20007829006002015 46-47 0.18957758591470106 48-49 0.1975528042529181 50-51 0.2033680676245347 52-53 0.2102466934412469 54-55 0.20871810992642198 56-57 0.1797747133739759 58-59 0.17106843335475566 60-61 0.17784736894224012 62-63 0.17964179306833897 64-65 0.17645170573305216 66-67 0.18804900239987613 68-69 0.17701661703200922 70-71 0.18303126086208124 72-73 0.19366488531303727 74-75 0.18329710147335512 76-77 0.19137201004079987 78-79 0.189012674615744 80-81 0.17861166069965262 82-83 0.19100647920029826 84-85 0.17518896282950114 86-87 0.17927626222783735 88-89 0.18568966697482026 90-91 0.1833635616261736 92-93 0.18878006408087933 94-95 0.18774993171219298 96-97 0.18057223520779764 98-99 0.18678625949632507 100-101 0.17312869809212839 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1504661.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.42508336430464 #Duplication Level Percentage of deduplicated Percentage of total 1 72.71649950775766 33.03153052100309 2 13.830582110952902 12.56510690733792 3 5.190209573945072 7.072971077240009 4 2.540246175273405 4.615635771106017 5 1.4270446723327619 3.2411811602651253 6 0.8737450146043118 2.3813964076527863 7 0.596896378995761 1.8979847443013815 8 0.4289110696116777 1.5586656874386824 9 0.3262997763887375 1.3339975089791127 >10 1.8264196473884609 15.56757022708099 >50 0.15365703061327338 4.807230519053783 >100 0.08332754459692715 6.870245936740564 >500 0.0035208557365969066 1.1498671992042393 >1k 0.002200534835373067 1.7018434386136114 >5k 1.4670232235820447E-4 0.5182565753817079 >10k+ 2.9340464471640893E-4 1.6865163186010366 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14547 0.9667958430503615 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10761 0.7151777044796137 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7777 0.5168606084692831 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2864 0.19034187767211352 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2466 0.16389073685036032 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2345 0.1558490583593248 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2295 0.152526050718401 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2089 0.1388352592377951 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1913 0.1271382723417434 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1663 0.11052323413712457 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1644 0.10926049123357355 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.646015281847539E-5 0.0 4 0.0 0.0 0.0 6.646015281847539E-5 0.0 5 0.0 0.0 0.0 6.646015281847539E-5 0.0 6 0.0 0.0 0.0 6.646015281847539E-5 0.0 7 0.0 0.0 0.0 1.3292030563695077E-4 0.0 8 0.0 0.0 0.0 1.3292030563695077E-4 0.0 9 0.0 0.0 0.0 2.6584061127390155E-4 0.0 10-11 0.0 0.0 0.0 2.6584061127390155E-4 0.0 12-13 0.0 0.0 0.0 2.6584061127390155E-4 0.0 14-15 0.0 0.0 0.0 2.9907068768313924E-4 0.0 16-17 0.0 0.0 0.0 3.3230076409237694E-4 0.0 18-19 0.0 0.0 0.0 3.3230076409237694E-4 0.0 20-21 0.0 0.0 0.0 3.3230076409237694E-4 0.0 22-23 0.0 0.0 0.0 5.316812225478031E-4 0.0 24-25 0.0 0.0 0.0 5.316812225478031E-4 0.0 26-27 0.0 0.0 0.0 9.96902292277131E-4 0.0 28-29 0.0 0.0 0.0 0.0015618135912341719 0.0 30-31 0.0 0.0 0.0 0.0032897775645145317 0.0 32-33 0.0 0.0 0.0 0.005316812225478031 0.0 34-35 0.0 0.0 0.0 0.006845395740302965 0.0 36-37 0.0 0.0 0.0 0.009337651470995793 0.0 38-39 0.0 0.0 0.0 0.012627429035510323 0.0 40-41 0.0 0.0 0.0 0.019074063858902437 0.0 42-43 0.0 0.0 0.0 0.02744804311403034 0.0 44-45 0.0 0.0 0.0 0.04555843475706488 0.0 46-47 0.0 0.0 0.0 0.08420501362100832 0.0 48-49 0.0 0.0 0.0 0.1161058869738765 0.0 50-51 0.0 0.0 0.0 0.15661335011673727 0.0 52-53 0.0 0.0 0.0 0.2100805430592007 0.0 54-55 0.0 0.0 0.0 0.27268600701420453 0.0 56-57 0.0 0.0 0.0 0.3392126199854984 0.0 58-59 0.0 0.0 0.0 0.4403317424988087 0.0 60-61 0.0 0.0 0.0 0.5726871368368025 0.0 62-63 0.0 0.0 0.0 0.7219898701435075 0.0 64-65 0.0 0.0 0.0 0.8464032762196934 0.0 66-67 0.0 0.0 0.0 1.0349839598421173 0.0 68-69 0.0 0.0 0.0 1.2617127711823461 0.0 70-71 0.0 0.0 0.0 1.4470701373930739 0.0 72-73 3.3230076409237694E-5 0.0 0.0 1.7619583414470101 0.0 74-75 6.646015281847539E-5 0.0 0.0 2.0169659478115003 0.0 76-77 6.646015281847539E-5 0.0 0.0 2.253298251233999 0.0 78-79 6.646015281847539E-5 0.0 0.0 2.56579388978647 0.0 80-81 6.646015281847539E-5 0.0 0.0 2.881878376591139 0.0 82-83 6.646015281847539E-5 0.0 0.0 3.253158020311552 0.0 84-85 6.646015281847539E-5 0.0 0.0 3.762708011970803 0.0 86-87 6.646015281847539E-5 0.0 0.0 4.39530897657346 0.0 88-89 6.646015281847539E-5 0.0 0.0 5.122216898025535 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTTGCG 60 0.003939366 31.686295 5 GTATCAA 17955 0.0 29.678425 1 GGTATCA 12305 0.0 25.76706 1 GTACATG 12480 0.0 25.672369 1 TACATGG 12350 0.0 25.663757 2 AGTACTT 11320 0.0 24.486435 12-13 ACATGGG 12615 0.0 24.25747 3 ATCAACG 22035 0.0 24.000978 3 TATCAAC 22165 0.0 23.9682 2 TCAACGC 22105 0.0 23.930561 4 CAACGCA 22275 0.0 23.72738 5 GAGTACT 11205 0.0 23.677856 12-13 AACGCAG 22640 0.0 23.396908 6 CATATAG 245 2.9649527E-10 23.273518 3 ATGGGCG 190 3.2687421E-7 22.513947 5 GTACTTT 11925 0.0 22.167841 14-15 CATGGGA 8875 0.0 22.010082 4 TACTTTT 12890 0.0 21.466421 14-15 ACGCAGA 24580 0.0 21.433605 7 CGCAGAG 24580 0.0 21.316935 8 >>END_MODULE