FastQCFastQC Report
Thu 26 May 2016
SRR937413_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937413_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504661
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135620.9013325925241632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109470.7275392929038501No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76980.5116102563966236No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32850.21832160200869166No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31320.20815319862746492No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA26600.17678400649714454No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25610.17020445136811546No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19290.12820163478683902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17120.11377978162522986No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16260.10806420848284098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG4950.026.8733225
GTACATG134550.026.7122121
TACATGG135900.025.9739422
TAACGCG559.4855015E-525.90753476-77
GTATCAA198000.024.707111
ACATGGG136200.024.6261273
ATGGGCG1551.4090019E-624.5203595
CCTATAC3700.024.39613
GTAAGGT4750.024.004144
GAGTACT105750.023.85121312-13
GGTATCA146900.023.8192331
AGTACTT106900.022.90589712-13
ACGTAAC1252.2430893E-422.819861
ATGGGAG33150.022.7867345
GTACTTT112200.022.3939314-15
CATGGGA98250.022.3880844
CATGGGG35450.021.7103734
TACCTGG10550.021.6151032
CTAAGGG4200.021.49182
ATGGGAT36800.021.172135
Produced by FastQC (version 0.11.5)