##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937413_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1504661 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17684847284538 33.0 31.0 34.0 31.0 34.0 2 32.374562110668116 34.0 31.0 34.0 31.0 34.0 3 32.43974290554484 34.0 31.0 34.0 31.0 34.0 4 36.05938081734025 37.0 35.0 37.0 35.0 37.0 5 35.971963119932 37.0 35.0 37.0 35.0 37.0 6 36.07109109626687 37.0 35.0 37.0 35.0 37.0 7 36.024168234572436 37.0 35.0 37.0 35.0 37.0 8 36.01357448621317 37.0 35.0 37.0 35.0 37.0 9 37.69037145244012 39.0 37.0 39.0 35.0 39.0 10-11 37.627195428073165 39.0 37.0 39.0 35.0 39.0 12-13 37.55422350948154 39.0 37.0 39.0 35.0 39.0 14-15 38.70056378147636 40.0 38.0 41.0 34.5 41.0 16-17 38.702255524666356 40.0 38.0 41.0 34.5 41.0 18-19 38.78347880353116 40.0 38.0 41.0 35.0 41.0 20-21 38.713590968331076 40.0 38.0 41.0 34.5 41.0 22-23 38.5954749940352 40.0 38.0 41.0 34.0 41.0 24-25 38.45117006422045 40.0 38.0 41.0 34.0 41.0 26-27 38.364719029734935 40.0 38.0 41.0 34.0 41.0 28-29 38.251728462424424 40.0 38.0 41.0 34.0 41.0 30-31 38.245341974039334 40.0 37.5 41.0 34.0 41.0 32-33 38.101756475378835 40.0 37.0 41.0 34.0 41.0 34-35 37.953272198854094 40.0 37.0 41.0 33.5 41.0 36-37 37.76271399338456 40.0 37.0 41.0 33.0 41.0 38-39 37.60094532921369 40.0 36.5 41.0 33.0 41.0 40-41 37.39972193072061 40.0 36.0 41.0 33.0 41.0 42-43 37.12047398051787 40.0 36.0 41.0 32.5 41.0 44-45 36.70603544585791 40.0 36.0 41.0 31.0 41.0 46-47 36.18069053427982 40.0 35.5 41.0 30.0 41.0 48-49 35.727343235453034 39.0 35.0 41.0 28.5 41.0 50-51 35.462284527877046 39.0 35.0 41.0 27.0 41.0 52-53 35.20863437013387 39.0 35.0 41.0 26.0 41.0 54-55 34.97713970123503 39.0 35.0 40.0 26.0 41.0 56-57 34.74039767097041 38.5 35.0 40.0 25.0 41.0 58-59 34.50556570549779 38.0 34.0 40.0 24.0 41.0 60-61 34.18576642845132 38.0 34.0 40.0 23.0 41.0 62-63 33.8225261371166 37.5 34.0 40.0 22.0 41.0 64-65 33.467499988369475 37.0 33.5 40.0 21.5 41.0 66-67 33.0496317775233 36.0 33.0 39.0 19.5 41.0 68-69 32.65949672384677 36.0 33.0 39.0 18.5 40.0 70-71 32.251836460172754 35.0 32.0 38.5 16.5 40.0 72-73 31.817412360657983 35.0 32.0 38.0 12.5 40.0 74-75 31.380044408674113 35.0 31.0 37.0 8.0 39.0 76-77 30.02885367534614 34.0 29.5 35.5 7.0 38.5 78-79 30.634120908297618 34.5 31.0 36.0 4.5 39.0 80-81 30.482309968823543 35.0 31.0 36.0 2.0 37.5 82-83 30.154535141138105 34.0 31.0 35.5 2.0 37.0 84-85 29.80141174656617 34.0 30.5 35.0 2.0 37.0 86-87 29.438724071402127 34.0 30.0 35.0 2.0 36.0 88-89 29.046692909565678 34.0 29.5 35.0 2.0 36.0 90-91 28.61891150232511 34.0 29.0 35.0 2.0 35.5 92-93 28.28503297420482 34.0 29.0 35.0 2.0 35.0 94-95 27.91521977375635 34.0 29.0 35.0 2.0 35.0 96-97 27.476537572250493 33.5 27.5 35.0 2.0 35.0 98-99 27.0528172126479 33.0 27.0 35.0 2.0 35.0 100-101 25.27755853311809 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1601616201013485 1101 2 0.19771667257582948 1101 3 0.15147463810443185 1101 4 0.12777774073927617 1101 5 -0.01324194608202589 1101 6 0.054451973644411567 1101 7 0.02381617455004914 1101 8 0.057809226294253335 1101 9 0.09142924052722634 1101 10-11 0.09152446670983494 1101 12-13 0.07759524078475977 1101 14-15 0.09939623083685234 1101 16-17 0.0646254227734886 1101 18-19 0.0715633246195182 1101 20-21 0.14269376294151925 1101 22-23 0.19622558488402575 1101 24-25 0.25615663111893383 1101 26-27 0.2662154772544554 1101 28-29 0.14149918307216325 1101 30-31 0.24963772173261845 1101 32-33 0.2337202634409863 1101 34-35 0.164314809468884 1101 36-37 0.26835091025115787 1101 38-39 0.24004981718248075 1101 40-41 0.18435553478234112 1101 42-43 0.4506162806205509 1101 44-45 0.41700873498704283 1101 46-47 0.43442091331905175 1101 48-49 0.35305353783471816 1101 50-51 0.15036877622426204 1101 52-53 0.15480117909990554 1101 54-55 0.25637949308715946 1101 56-57 0.3737275669355995 1101 58-59 0.15955322133707028 1101 60-61 0.1151777483428944 1101 62-63 0.11497146694011207 1101 64-65 0.12038975919437434 1101 66-67 0.1110836224655074 1101 68-69 0.13162825652977972 1101 70-71 -0.022857649429305127 1101 72-73 -0.04235247398542441 1101 74-75 0.05036114900963895 1101 76-77 0.05153076784474209 1101 78-79 -0.032560201085257034 1101 80-81 -0.12681413357760363 1101 82-83 -0.13684441317256102 1101 84-85 -0.12260015001260527 1101 86-87 -0.03648562185723847 1101 88-89 -0.10337236794789817 1101 90-91 -0.014537450682890807 1101 92-93 -0.12925154621559543 1101 94-95 -0.29261830072591977 1101 96-97 -0.30408627959954515 1101 98-99 -0.05424293170037586 1101 100-101 0.03467718234713857 1102 1 0.17147380794593658 1102 2 0.21133799968227152 1102 3 0.21981977159842359 1102 4 0.09362163933273848 1102 5 -0.024007285043509796 1102 6 0.01513074483106891 1102 7 0.026087393633879685 1102 8 0.029489838605343266 1102 9 0.058708118489114725 1102 10-11 0.17114536471952135 1102 12-13 0.2061850592169634 1102 14-15 0.2085973624547961 1102 16-17 0.10821766111179443 1102 18-19 0.17333509504278055 1102 20-21 0.1902726094695808 1102 22-23 0.24348410569771772 1102 24-25 0.2858930359866818 1102 26-27 0.09322761670092206 1102 28-29 0.08618960972405176 1102 30-31 0.25922803412503725 1102 32-33 0.21237070426305849 1102 34-35 0.23714599981320816 1102 36-37 0.27228113712359203 1102 38-39 0.1874530334123108 1102 40-41 0.25756343215837063 1102 42-43 0.5076237798347094 1102 44-45 0.42259447700011066 1102 46-47 0.5313094629221382 1102 48-49 0.5959766846107541 1102 50-51 0.6062067373161639 1102 52-53 0.6733855589802573 1102 54-55 0.6297001530978434 1102 56-57 0.5914707176034426 1102 58-59 0.6269204133977766 1102 60-61 0.6076411129603727 1102 62-63 0.5792516571397428 1102 64-65 0.5988855256060788 1102 66-67 0.7236002749121013 1102 68-69 0.7028323718669967 1102 70-71 0.5141255010389543 1102 72-73 0.6752035705408481 1102 74-75 0.5906488434129216 1102 76-77 0.6977419932153381 1102 78-79 0.7175932259508322 1102 80-81 0.610381070517775 1102 82-83 0.6839069053020452 1102 84-85 0.6823683912081258 1102 86-87 0.646751663656751 1102 88-89 0.7436451601554417 1102 90-91 0.6483002759026455 1102 92-93 0.7753263173815306 1102 94-95 0.9200061248582259 1102 96-97 0.8470361077750326 1102 98-99 0.9897714180933086 1102 100-101 1.2177612902998973 1103 1 0.023564647915975456 1103 2 0.07195077023232699 1103 3 0.042155208034571956 1103 4 0.0023011117947717707 1103 5 -0.1313587859726013 1103 6 -0.05722634656147818 1103 7 -0.025746109122522398 1103 8 -0.0031555474618798485 1103 9 -1.243325499373782E-4 1103 10-11 -0.08860442677892166 1103 12-13 0.0020098508730512776 1103 14-15 0.012614697039971645 1103 16-17 -0.0195226865025262 1103 18-19 -0.07389730196674549 1103 20-21 -0.014430282869277278 1103 22-23 -0.014199919117373838 1103 24-25 -0.0797451778925975 1103 26-27 0.03282140622537355 1103 28-29 -0.11384095612244494 1103 30-31 0.043558607239639 1103 32-33 -0.06578314515849826 1103 34-35 -0.07724613311537354 1103 36-37 -0.13535425115723854 1103 38-39 0.08326962771326407 1103 40-41 -0.03521755210171307 1103 42-43 0.049988009895635344 1103 44-45 0.05294536694414376 1103 46-47 -0.08651739499844524 1103 48-49 -0.03930290659677382 1103 50-51 -0.08801615175703148 1103 52-53 -0.006577582790505687 1103 54-55 -0.0897586422493788 1103 56-57 -0.15062306611315535 1103 58-59 -0.053370788517021595 1103 60-61 -0.045145473378845224 1103 62-63 -0.028917753981509975 1103 64-65 -0.07952974311284322 1103 66-67 -0.08107052494731448 1103 68-69 0.05428600852238219 1103 70-71 0.089254094973505 1103 72-73 0.1077234076424709 1103 74-75 -0.12335837888839762 1103 76-77 -0.09749653820359683 1103 78-79 -0.10233809547941064 1103 80-81 0.013765449810392738 1103 82-83 -0.13934567246745644 1103 84-85 -0.041908258575968915 1103 86-87 -0.13166337274846285 1103 88-89 -0.1918122837897478 1103 90-91 0.04786591564316112 1103 92-93 0.009271863712019268 1103 94-95 0.009137261632258031 1103 96-97 -0.1278564222440508 1103 98-99 -0.11754440608769912 1103 100-101 0.07733545263474184 1104 1 0.13642768208169542 1104 2 0.15406587892503154 1104 3 0.04140719232270129 1104 4 0.04523515105383069 1104 5 0.042610138341487414 1104 6 -0.08273332782660248 1104 7 -0.04274002057757542 1104 8 -0.003717606661147954 1104 9 0.055802169185867 1104 10-11 -0.013297034540237007 1104 12-13 -0.018789723457338425 1104 14-15 -0.005527020514968228 1104 16-17 -0.00384803498567976 1104 18-19 -0.0421395069404511 1104 20-21 -0.04982760457984625 1104 22-23 -0.04077075211230863 1104 24-25 -0.056720571043982204 1104 26-27 -0.07007442332115232 1104 28-29 -0.045404125259715045 1104 30-31 -0.023029293791530847 1104 32-33 -0.03837985865913396 1104 34-35 -0.04680356950664333 1104 36-37 -0.03763781002327704 1104 38-39 -0.033120134558899395 1104 40-41 -0.03135613450075425 1104 42-43 0.06656778942920738 1104 44-45 0.06220613018444254 1104 46-47 0.24494042026712748 1104 48-49 0.01583771133316958 1104 50-51 -0.07396112667707655 1104 52-53 -0.09467262751675776 1104 54-55 -0.04374987489934057 1104 56-57 0.06066140029800238 1104 58-59 0.08999539820781166 1104 60-61 0.15414076944877309 1104 62-63 0.11264575032868152 1104 64-65 -0.20464221940903116 1104 66-67 0.0781340323730575 1104 68-69 0.08551074969798833 1104 70-71 -0.025856370285175956 1104 72-73 0.09864118843681169 1104 74-75 -0.032427094492888386 1104 76-77 0.06042372673769947 1104 78-79 0.1261481540516698 1104 80-81 0.16017784270008661 1104 82-83 -0.05933554637798366 1104 84-85 0.04363189836230319 1104 86-87 0.12412109946376759 1104 88-89 -0.03167343196324879 1104 90-91 0.08097100911128408 1104 92-93 0.19004608736775097 1104 94-95 0.09855124401505222 1104 96-97 0.1507599323895228 1104 98-99 0.1290584230295515 1104 100-101 0.5445018814575491 1105 1 0.030197318450305488 1105 2 0.1963779844901552 1105 3 0.1499420118730086 1105 4 0.09943016239169822 1105 5 -0.040062046998514234 1105 6 0.04692271129587766 1105 7 0.12880192953580405 1105 8 0.06782707546873468 1105 9 0.1361671665904538 1105 10-11 0.10484355502892129 1105 12-13 0.05518508735051597 1105 14-15 0.1191444545007414 1105 16-17 0.1330847300882212 1105 18-19 0.20645760258609158 1105 20-21 0.11125003932912136 1105 22-23 0.008899356895142319 1105 24-25 -0.0015433997738725225 1105 26-27 -0.004971836101525184 1105 28-29 2.5128549895470087E-4 1105 30-31 0.035082063201059555 1105 32-33 0.06324091013031108 1105 34-35 0.09180597069498475 1105 36-37 0.16701822909919883 1105 38-39 0.03873258243150701 1105 40-41 0.22588575824027401 1105 42-43 0.10565953054332056 1105 44-45 0.3026727586028741 1105 46-47 0.37107693913170436 1105 48-49 0.3305721593292361 1105 50-51 0.27300633500639293 1105 52-53 0.3225267823676745 1105 54-55 0.32617062342046665 1105 56-57 0.40378969579290924 1105 58-59 0.3177861530639703 1105 60-61 0.19255906849529936 1105 62-63 0.3080600606311137 1105 64-65 0.3262705473040768 1105 66-67 0.2179238037032789 1105 68-69 0.15591686395091386 1105 70-71 0.16864339749342605 1105 72-73 0.3215887610401502 1105 74-75 0.31412646669062383 1105 76-77 0.17912233067727001 1105 78-79 0.10158664270014128 1105 80-81 0.11474440828214227 1105 82-83 0.05569669141673472 1105 84-85 0.11647838635508734 1105 86-87 0.21452011047379216 1105 88-89 0.30999391770754414 1105 90-91 0.12854771047456026 1105 92-93 0.201779415237052 1105 94-95 0.22426976435491852 1105 96-97 0.31618626356456403 1105 98-99 0.5514035859943327 1105 100-101 0.8493744328877604 1106 1 -0.13856798021136285 1106 2 0.032002805674622437 1106 3 0.06044523849983108 1106 4 0.04705549220700078 1106 5 -0.021085348648384183 1106 6 -0.0527911838625954 1106 7 0.07642725935110661 1106 8 -0.027175626632548244 1106 9 0.03786949039755427 1106 10-11 -0.14126462439060816 1106 12-13 -0.16668683304875742 1106 14-15 0.07507954049113863 1106 16-17 0.045322864684649744 1106 18-19 0.028076002306342218 1106 20-21 0.15887219020174115 1106 22-23 0.02176851161428317 1106 24-25 -0.05527865742717353 1106 26-27 0.10115954959959339 1106 28-29 -0.07539562933715871 1106 30-31 0.0600542474525767 1106 32-33 0.08844197825505518 1106 34-35 -0.0746426734859611 1106 36-37 0.11169747378241368 1106 38-39 0.10829659044244266 1106 40-41 0.00670799176540271 1106 42-43 -0.27513806617601233 1106 44-45 -0.08586000040147468 1106 46-47 -0.10928196349728125 1106 48-49 0.03759205951043754 1106 50-51 0.15338033761312886 1106 52-53 0.08097084887115358 1106 54-55 -0.040649162318800336 1106 56-57 0.07219895287400391 1106 58-59 -0.05359541542635071 1106 60-61 0.04213070596868107 1106 62-63 -0.021461190600724933 1106 64-65 0.022081495582682464 1106 66-67 -0.09186892923374756 1106 68-69 -0.011881241399244402 1106 70-71 0.03487825777889242 1106 72-73 0.07752702597323236 1106 74-75 -0.14029464784668733 1106 76-77 -0.12043023314000223 1106 78-79 0.07977432747618707 1106 80-81 0.16172520746833285 1106 82-83 0.09106322350816143 1106 84-85 -0.042034822151759244 1106 86-87 0.12888734193261087 1106 88-89 0.24231184376533577 1106 90-91 0.10643260095998386 1106 92-93 0.15548129399217814 1106 94-95 -0.3931031574113035 1106 96-97 -0.1140645550131758 1106 98-99 0.16071470911478158 1106 100-101 0.5199048394745276 1107 1 0.082535294891386 1107 2 0.060748477615071295 1107 3 0.049630729569074106 1107 4 0.03195804343258857 1107 5 0.010676968543208432 1107 6 0.05989579321462912 1107 7 0.04497293325327689 1107 8 0.1427878852506126 1107 9 0.18279008950889164 1107 10-11 0.0631149304936045 1107 12-13 -0.05094986136628421 1107 14-15 0.01816469904769491 1107 16-17 -0.11143871685942486 1107 18-19 0.01575464204503163 1107 20-21 0.16951357675018386 1107 22-23 0.1577068545437328 1107 24-25 0.07344764246199276 1107 26-27 0.022622556338113498 1107 28-29 0.0652454629522694 1107 30-31 -0.047537634922285577 1107 32-33 0.014735737021517537 1107 34-35 0.1840798991224446 1107 36-37 0.022277556371108176 1107 38-39 0.0421510891722221 1107 40-41 0.2252318414579051 1107 42-43 0.2651334858440748 1107 44-45 0.201555277526154 1107 46-47 0.09269202400652432 1107 48-49 -0.12621334700838105 1107 50-51 -0.09892003217235867 1107 52-53 0.013118890577466402 1107 54-55 0.01053596583100358 1107 56-57 0.0808712562354259 1107 58-59 0.17082955343123984 1107 60-61 0.23924917464480444 1107 62-63 0.20279065587454426 1107 64-65 0.21201538539249043 1107 66-67 0.3911300114338232 1107 68-69 0.18338584769256983 1107 70-71 0.24891392197051943 1107 72-73 0.44775439009913853 1107 74-75 0.2839645551037506 1107 76-77 0.2320145687746411 1107 78-79 0.08084493982917706 1107 80-81 0.08887550516035958 1107 82-83 0.1465911241663207 1107 84-85 0.25190074493438885 1107 86-87 0.23437723413572797 1107 88-89 0.24643858111955552 1107 90-91 0.2265993967736577 1107 92-93 0.26485663116395486 1107 94-95 0.4149850418216623 1107 96-97 0.365019514785061 1107 98-99 0.4422980187439194 1107 100-101 0.5906775636263291 1108 1 -0.09226340029787394 1108 2 -0.08755408985774693 1108 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-0.3623964024771311 2316 60-61 -0.45491778691455664 2316 62-63 -0.21710931165686276 2316 64-65 -0.32205730778655095 2316 66-67 -0.19329053352788605 2316 68-69 -0.07859488274144866 2316 70-71 -0.3223068608510431 2316 72-73 -0.18804388140401684 2316 74-75 -0.2873547279604054 2316 76-77 -0.1593801581790366 2316 78-79 -0.3980811156738824 2316 80-81 -0.2577519559776462 2316 82-83 -0.3173669074340175 2316 84-85 -0.44587278476708647 2316 86-87 -0.4703912720533694 2316 88-89 -0.49089994232532774 2316 90-91 -0.377367609293227 2316 92-93 -0.2636310982595269 2316 94-95 0.0012989803356191487 2316 96-97 -0.002173337745482229 2316 98-99 -0.2285294927386552 2316 100-101 -0.7720991946942064 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 17.0 5 34.0 6 85.0 7 188.0 8 398.0 9 790.0 10 1505.0 11 2643.0 12 4281.0 13 6308.0 14 7628.0 15 11488.0 16 16676.0 17 24221.0 18 21069.0 19 10338.0 20 8038.0 21 7775.0 22 7891.0 23 8257.0 24 9276.0 25 10945.0 26 12821.0 27 15919.0 28 19755.0 29 24602.0 30 30426.0 31 38465.0 32 48813.0 33 65149.0 34 90769.0 35 131648.0 36 203640.0 37 325421.0 38 304048.0 39 33326.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.15901351666759 20.288167612795714 15.50589920384188 20.04691966669482 2 15.2396453420405 22.240956600855608 40.02090836407669 22.4984896930272 3 19.167506833765213 27.733356550080053 30.793381366301116 22.305755249853622 4 12.242225989774441 17.839632980452073 37.922429038833336 31.99571199094015 5 12.217407517453582 40.02482021298588 33.17639567168455 14.581376597875991 6 29.283672534876626 37.915783023551484 17.743664519782197 15.056879921789692 7 27.046291490242652 35.52487902590683 20.506678913057495 16.922150570793022 8 27.305951307304436 33.79226284192918 19.66901514693343 19.23277070383296 9 28.021328392242506 16.40209987498845 20.56868623563713 35.007885497131916 10-11 24.50415741485956 28.099551992109852 26.534714463922437 20.86157612910815 12-13 26.53792242646106 25.984091569183 27.600057423480223 19.87792858087572 14-15 23.180935772243714 26.247573373670214 27.852220533395894 22.719270320690175 16-17 20.446432784527545 30.005795325325774 28.06004143125927 21.487730458887416 18-19 20.373991217955407 27.58937062899882 32.37809048018125 19.65854767286452 20-21 21.87469469867299 26.14309801343957 32.31412258309347 19.66808470479397 22-23 21.25058485439784 26.589973203034862 32.112361163541244 20.047080779026057 24-25 20.959387767648117 26.608447437373624 32.52879808195553 19.90336671302273 26-27 20.892943304786122 26.78115458693829 32.44080651743072 19.885095590844866 28-29 20.354948651213736 27.038465730700008 32.67962105845228 19.926964559633976 30-31 20.466869281519227 26.950954401024553 32.5581310341665 20.024045283289723 32-33 20.304208057496005 27.031470876164132 32.14494826409403 20.519372802245822 34-35 20.81359189877321 26.780251498510292 32.2494900844775 20.156666518238993 36-37 21.130407447258882 26.527204466653952 32.10836194996747 20.2340261361197 38-39 20.85326861000584 26.77280796139463 32.2009077127672 20.17301571583234 40-41 20.703533885705816 27.018743756899394 32.22925296794427 20.048469389450513 42-43 20.63462943280443 27.278576636974496 31.56855355192673 20.51824037829434 44-45 20.743243827014858 27.44604930944578 31.120232397862374 20.690474465676985 46-47 21.17809260690614 27.784231797062592 30.2724666885099 20.765208907521362 48-49 21.287186947757668 28.08097637939709 29.71210791002093 20.919728762824317 50-51 21.42014048347103 28.391444983288594 29.508174931097436 20.68023960214294 52-53 21.400900269230082 28.684933018134984 29.143375152276825 20.770791560358116 54-55 20.84316666677743 28.52974856130384 29.32417999801949 21.302904773899236 56-57 21.281604294920918 28.909834175272703 28.795954703418246 21.012606826388136 58-59 21.496137668218953 28.2400155250917 28.952933584375483 21.31091322231386 60-61 21.181814375463976 28.4177299737283 29.582178311260808 20.818277339546913 62-63 21.414856901321958 28.2896612592471 29.588060034785247 20.707421804645698 64-65 21.120538114565342 28.46348778894382 29.492756175643553 20.923217920847286 66-67 21.042613585385677 28.54064802636607 29.128654228427532 21.288084159820716 68-69 21.118544309980784 28.602821499327757 29.09246667521787 21.186167515473585 70-71 21.037030932548927 28.76481812182279 28.957153804079454 21.240997141548828 72-73 20.985113243228785 28.63119529700906 28.739259613221463 21.644431846540694 74-75 20.994928425738422 29.13174462553359 28.534434002077546 21.338892946650443 76-77 20.971102460953 28.574941465220405 28.975995257403497 21.4779608164231 78-79 20.900322398201325 28.56909297177238 28.800274613351444 21.730310016674853 80-81 20.985590774267425 29.139420773184128 28.565570583673 21.309417868875446 82-83 20.97605860751329 29.02365893542932 28.48077647112774 21.51950598592965 84-85 20.837862383555915 28.722480516084097 28.42147798291749 22.018179117442497 86-87 20.847586266939864 28.578762924007467 28.432583817883234 22.14106699116944 88-89 20.888891251916544 28.26227967628589 28.49628587435974 22.35254319743783 90-91 20.672811534671645 28.18816124213405 28.71873353904849 22.420293684145808 92-93 20.619966423996075 28.30519603946709 28.78045733016271 22.29438020637412 94-95 20.24927874119154 28.240945967231156 28.823735047296367 22.68604024428094 96-97 20.274732979721012 28.001722647161053 28.557130144265052 23.166414228852876 98-99 20.143667342014606 28.281438321636866 28.305197897327396 23.269696439021132 100-101 19.985024666428266 28.377958010573796 28.631535008248733 23.00548231474921 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1147.0 1 786.0 2 563.0 3 689.5 4 621.0 5 697.0 6 1199.5 7 2326.5 8 3564.0 9 4223.0 10 4019.0 11 3634.0 12 3766.5 13 3985.5 14 4202.0 15 4371.5 16 4496.5 17 4653.0 18 4836.5 19 5034.0 20 5411.5 21 6003.0 22 6711.5 23 7430.0 24 8342.0 25 9911.5 26 11865.0 27 13766.0 28 15489.5 29 17712.0 30 20711.0 31 23411.5 32 26939.5 33 30678.0 34 34615.5 35 37717.0 36 41305.0 37 45563.5 38 47100.5 39 47484.0 40 47784.5 41 48908.5 42 49791.0 43 51550.5 44 54711.5 45 56093.5 46 54603.0 47 55824.5 48 67195.5 49 65428.5 50 52208.5 51 46384.0 52 41314.0 53 38938.0 54 37542.0 55 34441.0 56 30366.0 57 27000.0 58 25203.0 59 22953.5 60 19224.0 61 15772.5 62 12779.5 63 9224.0 64 6128.0 65 4497.5 66 3250.0 67 2434.5 68 1783.0 69 1134.5 70 817.5 71 635.5 72 536.0 73 549.0 74 436.5 75 286.5 76 208.5 77 125.0 78 69.0 79 45.5 80 30.5 81 18.0 82 11.5 83 6.5 84 2.5 85 0.5 86 0.5 87 2.0 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05409856439423896 2 0.0 3 0.0 4 0.0 5 0.016083356982071046 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003323007640923769 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.6398198664018E-4 24-25 2.3261053486466385E-4 26-27 0.0012627429035510325 28-29 0.0032565474881052943 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014887074231338487 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 9.96902292277131E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.984511461385655E-4 84-85 0.0014953534384156964 86-87 0.0 88-89 0.0 90-91 0.010201633457635972 92-93 9.96902292277131E-4 94-95 0.0 96-97 0.0 98-99 2.9907068768313924E-4 100-101 0.012428048577054897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1504661.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.85567680223567 #Duplication Level Percentage of deduplicated Percentage of total 1 71.27407223192664 31.257726761825744 2 14.360875937887101 12.596118674579596 3 5.511219997646404 7.250948490083961 4 2.690976385992325 4.720583626661103 5 1.5560115708442575 3.4119970275742393 6 0.934340913642996 2.458569187909969 7 0.6478624673889842 1.9888712887477167 8 0.4550136533005193 1.5963945375801667 9 0.33493692520584595 1.3219996986865945 >10 1.9734173640585213 16.050047503276502 >50 0.16621309149374194 5.009646858728589 >100 0.08702417883235919 6.952668549347182 >500 0.005761145427376051 1.7577082955296464 >1k 0.001819309082329279 1.4853020734542584 >5k 1.516090901941066E-4 0.511833804622001 >10k+ 3.032181803882132E-4 1.6295836213926502 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13562 0.9013325925241632 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10947 0.7275392929038501 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7698 0.5116102563966236 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3285 0.21832160200869166 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3132 0.20815319862746492 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2660 0.17678400649714454 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2561 0.17020445136811546 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1929 0.12820163478683902 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1712 0.11377978162522986 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1626 0.10806420848284098 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.646015281847539E-5 0.0 10-11 0.0 0.0 0.0 6.646015281847539E-5 0.0 12-13 0.0 0.0 0.0 6.646015281847539E-5 0.0 14-15 0.0 0.0 0.0 6.646015281847539E-5 0.0 16-17 0.0 0.0 0.0 6.646015281847539E-5 0.0 18-19 0.0 0.0 0.0 6.646015281847539E-5 0.0 20-21 0.0 0.0 0.0 6.646015281847539E-5 0.0 22-23 0.0 0.0 0.0 1.9938045845542616E-4 0.0 24-25 0.0 0.0 0.0 1.9938045845542616E-4 0.0 26-27 0.0 0.0 0.0 6.646015281847539E-4 0.0 28-29 0.0 0.0 0.0 0.001196282750732557 0.0 30-31 0.0 0.0 0.0 0.0028910166476036796 0.0 32-33 0.0 0.0 0.0 0.004951281384976417 0.0 34-35 0.0 0.0 0.0 0.006479864899801351 0.0 36-37 0.0 0.0 0.0 0.008905660477675702 0.0 38-39 0.0 0.0 0.0 0.012295128271417946 0.0 40-41 6.646015281847539E-5 0.0 0.0 0.018874683400447012 0.0 42-43 6.646015281847539E-5 0.0 0.0 0.02728189273198415 0.0 44-45 6.646015281847539E-5 0.0 0.0 0.045226133992972505 0.0 46-47 6.646015281847539E-5 0.0 0.0 0.08450408430869147 0.0 48-49 6.646015281847539E-5 0.0 0.0 0.1169698689605167 0.0 50-51 6.646015281847539E-5 0.0 0.0 0.15804224340233447 0.0 52-53 6.646015281847539E-5 0.0 0.0 0.21180850703248105 0.0 54-55 6.646015281847539E-5 0.0 0.0 0.2747795018279865 0.0 56-57 6.646015281847539E-5 0.0 0.0 0.3422033268623298 0.0 58-59 6.646015281847539E-5 0.0 0.0 0.444851032890465 0.0 60-61 6.646015281847539E-5 0.0 0.0 0.5780371791386898 0.0 62-63 6.646015281847539E-5 0.0 0.0 0.7270076116813022 0.0 64-65 6.646015281847539E-5 0.0 0.0 0.8518197786743991 0.0 66-67 6.646015281847539E-5 0.0 0.0 1.0416299751239648 0.0 68-69 6.646015281847539E-5 0.0 0.0 1.2701864406667016 0.0 70-71 6.646015281847539E-5 0.0 0.0 1.4569062400102082 0.0 72-73 6.646015281847539E-5 0.0 0.0 1.7741205494127912 0.0 74-75 6.646015281847539E-5 0.0 0.0 2.031188420514654 0.0 76-77 6.646015281847539E-5 0.0 0.0 2.2696142187509345 0.0 78-79 6.646015281847539E-5 0.0 0.0 2.5852667145622834 0.0 80-81 6.646015281847539E-5 0.0 0.0 2.9054717308416977 0.0 82-83 6.646015281847539E-5 0.0 0.0 3.2827327883157733 0.0 84-85 6.646015281847539E-5 0.0 0.0 3.8003244584660596 0.0 86-87 6.646015281847539E-5 0.0 0.0 4.440634800795661 0.0 88-89 6.646015281847539E-5 0.0 0.0 5.1804359918945195 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 495 0.0 26.873322 5 GTACATG 13455 0.0 26.712212 1 TACATGG 13590 0.0 25.973942 2 TAACGCG 55 9.4855015E-5 25.907534 76-77 GTATCAA 19800 0.0 24.70711 1 ACATGGG 13620 0.0 24.626127 3 ATGGGCG 155 1.4090019E-6 24.520359 5 CCTATAC 370 0.0 24.3961 3 GTAAGGT 475 0.0 24.00414 4 GAGTACT 10575 0.0 23.851213 12-13 GGTATCA 14690 0.0 23.819233 1 AGTACTT 10690 0.0 22.905897 12-13 ACGTAAC 125 2.2430893E-4 22.81986 1 ATGGGAG 3315 0.0 22.786734 5 GTACTTT 11220 0.0 22.39393 14-15 CATGGGA 9825 0.0 22.388084 4 CATGGGG 3545 0.0 21.710373 4 TACCTGG 1055 0.0 21.615103 2 CTAAGGG 420 0.0 21.4918 2 ATGGGAT 3680 0.0 21.17213 5 >>END_MODULE