FastQCFastQC Report
Thu 26 May 2016
SRR937412_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937412_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662911
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151710.9123158124517787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112200.674720414983123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77620.4667718236273619No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32410.19489918582533883No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26590.1599003193796902No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26340.15839693164577057No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24950.15003809584517752No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23190.13945424619838342No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20700.12448050436854408No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18060.10860472989835294No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17570.10565808993987051No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138200.027.9575061
GTATCAA194550.027.8477151
GTACATG137650.026.2048381
TACATGG138050.025.6399962
ACATGGG138900.024.3201033
CATGGGA98300.023.0555784
ATCAACG235300.023.0187113
GAGTACT116200.022.99443812-13
TCAACGC236400.022.8921954
TATCAAC237450.022.8302942
CAACGCA238900.022.5922895
AGTACTT117800.022.3595312-13
AACGCAG243150.022.2156016
GTACTTT126000.021.12962714-15
TACCTGG11350.020.9301452
ATGGGAG36600.020.7700215
ATGGGAT38600.020.6785495
CGCAGAG263100.020.2776558
ACGCAGA264200.020.2471717
GCAGAGT269100.019.5071729
Produced by FastQC (version 0.11.5)