##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937412_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1662911 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91285703203599 33.0 31.0 34.0 30.0 34.0 2 31.98007109219916 33.0 31.0 34.0 30.0 34.0 3 32.00700518548497 33.0 31.0 34.0 30.0 34.0 4 35.636827827827226 37.0 35.0 37.0 33.0 37.0 5 35.672358893530685 37.0 35.0 37.0 35.0 37.0 6 35.72233150180617 37.0 35.0 37.0 35.0 37.0 7 35.62879252106697 37.0 35.0 37.0 33.0 37.0 8 35.644891398276876 37.0 35.0 37.0 33.0 37.0 9 37.26690424201897 39.0 37.0 39.0 34.0 39.0 10-11 37.16883916216803 39.0 37.0 39.0 34.0 39.0 12-13 37.031924438529785 39.0 37.0 39.0 33.0 39.0 14-15 38.17087865796786 40.0 38.0 41.0 33.0 41.0 16-17 38.137580423726824 40.0 38.0 41.0 33.0 41.0 18-19 38.18820700566657 40.0 38.0 41.0 33.5 41.0 20-21 38.12414615093652 40.0 38.0 41.0 33.5 41.0 22-23 37.98356346190506 40.0 38.0 41.0 33.0 41.0 24-25 37.91308193884099 40.0 38.0 41.0 33.0 41.0 26-27 37.91186239071123 40.0 38.0 41.0 33.0 41.0 28-29 37.82060164374401 40.0 37.5 41.0 33.0 41.0 30-31 37.55321150680945 40.0 37.0 41.0 33.0 41.0 32-33 37.25451542505883 39.5 36.0 41.0 32.0 41.0 34-35 37.2306635773051 40.0 36.0 41.0 32.0 41.0 36-37 37.07330157777536 40.0 36.0 41.0 31.5 41.0 38-39 36.93934973068312 40.0 36.0 41.0 31.0 41.0 40-41 36.6836324974698 39.5 35.5 41.0 30.5 41.0 42-43 36.3511282323588 39.0 35.0 41.0 30.0 41.0 44-45 35.99113241779025 39.0 35.0 41.0 29.5 41.0 46-47 35.45481057013875 39.0 35.0 41.0 26.5 41.0 48-49 34.986436435864576 39.0 35.0 41.0 24.0 41.0 50-51 33.97481434664874 38.0 34.0 39.5 22.5 40.5 52-53 34.061251624410446 38.0 34.0 39.5 23.0 40.5 54-55 34.43215030750293 39.0 35.0 40.0 22.5 41.0 56-57 34.24029217438576 38.0 34.0 40.0 21.0 41.0 58-59 34.00322627007699 38.0 34.0 40.0 19.5 41.0 60-61 33.694137569599334 38.0 34.0 40.0 18.0 41.0 62-63 33.396200999331896 37.5 33.5 40.0 16.0 41.0 64-65 32.98883584268792 37.0 33.0 40.0 12.5 41.0 66-67 32.644076862802635 36.5 32.5 39.0 8.0 41.0 68-69 32.188541659776135 36.0 32.0 39.0 7.0 40.5 70-71 31.729517995851854 35.0 31.5 39.0 2.0 40.0 72-73 31.288154928315464 35.0 31.0 38.0 2.0 40.0 74-75 30.819151175258327 35.0 31.0 37.0 2.0 39.0 76-77 30.349370772097846 35.0 30.0 37.0 2.0 39.0 78-79 29.870374902805985 34.5 30.0 36.0 2.0 39.0 80-81 29.400613141653402 34.0 29.0 36.0 2.0 37.5 82-83 28.948308418189548 34.0 29.0 35.0 2.0 37.0 84-85 28.453856820960354 34.0 29.0 35.0 2.0 36.5 86-87 28.016886051027384 34.0 27.5 35.0 2.0 36.0 88-89 27.57993692987779 34.0 26.5 35.0 2.0 36.0 90-91 27.11073262489694 33.0 26.0 35.0 2.0 35.0 92-93 26.667757925709793 33.0 25.0 35.0 2.0 35.0 94-95 26.18960665964685 33.0 24.5 35.0 2.0 35.0 96-97 25.52148160665243 32.5 21.5 35.0 2.0 35.0 98-99 24.700553727770156 32.0 10.0 34.5 2.0 35.0 100-101 22.262044390830297 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05818356301130123 1101 2 -0.07430594590971396 1101 3 -0.11520217957696843 1101 4 -0.1350750162191332 1101 5 -0.16874296405041633 1101 6 -0.33565304876881186 1101 7 -0.1443887823932215 1101 8 -0.2728671611308613 1101 9 -0.15349133779640312 1101 10-11 -0.2148664712890067 1101 12-13 -0.09253371346301265 1101 14-15 -0.21544476390079836 1101 16-17 -0.24922903147649578 1101 18-19 -0.08773576360940893 1101 20-21 -0.058029921754524594 1101 22-23 -0.18787562381007206 1101 24-25 1.6293378907050737E-4 1101 26-27 -0.2898026738702413 1101 28-29 -0.20785419408705508 1101 30-31 -0.05819254340020308 1101 32-33 -0.2236578548791215 1101 34-35 -0.061448271331570936 1101 36-37 -0.08326941442450675 1101 38-39 0.011404642432786716 1101 40-41 -0.03860332906980801 1101 42-43 -0.02618592065965686 1101 44-45 0.13562657257440236 1101 46-47 0.3988443757590687 1101 48-49 0.2546235098502976 1101 50-51 0.2514418048278415 1101 52-53 0.2808096546318808 1101 54-55 0.1770638355708698 1101 56-57 0.2641066836222734 1101 58-59 0.253368063402462 1101 60-61 0.40872639874213235 1101 62-63 0.3450361232705035 1101 64-65 0.3093720869878851 1101 66-67 0.3732210521894146 1101 68-69 0.3583032079917743 1101 70-71 0.2720267302032191 1101 72-73 0.3544549163284749 1101 74-75 0.2877938922470662 1101 76-77 0.41530852771983007 1101 78-79 0.2227364427542753 1101 80-81 0.3376255609301104 1101 82-83 0.44797562227039833 1101 84-85 0.399940248360803 1101 86-87 0.5083724009292858 1101 88-89 0.388672548739617 1101 90-91 0.34559375696766637 1101 92-93 0.39953168172864295 1101 94-95 0.24632937730668658 1101 96-97 0.30976845053321966 1101 98-99 0.4320235765869356 1101 100-101 0.4987123574517902 1102 1 -0.13717015019460632 1102 2 -0.10063480830415017 1102 3 -0.022306004713577465 1102 4 -0.1350750162191332 1102 5 -0.14191733066740397 1102 6 -0.18910560713940328 1102 7 -0.07434407300424795 1102 8 -0.13277774235292128 1102 9 -0.11027226179044192 1102 10-11 -0.06732548768245294 1102 12-13 6.108468946521839E-4 1102 14-15 0.09752095890099355 1102 16-17 0.01579829092787577 1102 18-19 -0.03781027925769109 1102 20-21 -9.0125806848107E-4 1102 22-23 -0.046295892066396505 1102 24-25 -0.1391813285060124 1102 26-27 -0.23913203303566632 1102 28-29 -0.21058643452422388 1102 30-31 -0.23553978632121897 1102 32-33 -0.29171548031379047 1102 34-35 -0.044061286731469806 1102 36-37 -0.2255943026510394 1102 38-39 0.029288398021456885 1102 40-41 0.019022105604811657 1102 42-43 -0.09796932850863982 1102 44-45 0.22752920546064104 1102 46-47 0.10202371008594469 1102 48-49 -0.25233128398974003 1102 50-51 -0.18621343610609387 1102 52-53 -0.24030311238252722 1102 54-55 -0.17986611971974042 1102 56-57 -0.16634537797733628 1102 58-59 -0.29904127589212237 1102 60-61 -0.10394126146650962 1102 62-63 -0.015867999928708798 1102 64-65 -0.15237654689189384 1102 66-67 -0.23134924090546605 1102 68-69 -0.24254130268880658 1102 70-71 -0.32683069652306074 1102 72-73 -0.26054756752646924 1102 74-75 -0.4320272701970467 1102 76-77 -0.39666365310480955 1102 78-79 -0.5027975860584419 1102 80-81 -0.4251662920256756 1102 82-83 -0.4342896534375029 1102 84-85 -0.519334465995879 1102 86-87 -0.41562163781885175 1102 88-89 -0.5666180622887005 1102 90-91 -0.5155587517258269 1102 92-93 -0.4705627047591854 1102 94-95 -0.28099302706489837 1102 96-97 -0.3221242469332495 1102 98-99 -0.5505894388129633 1102 100-101 -0.37560458243892114 1103 1 0.13136162901290405 1103 2 -0.03141078074345671 1103 3 0.01734924429792528 1103 4 0.13495515353114484 1103 5 -0.03461802816617876 1103 6 -0.02746844382291158 1103 7 0.07844248913856688 1103 8 0.08427483055731244 1103 9 0.155738505619766 1103 10-11 0.07805999448832068 1103 12-13 0.15352947742224643 1103 14-15 0.05699817811431984 1103 16-17 0.1710598226788136 1103 18-19 0.11866854335467991 1103 20-21 0.12400271973433519 1103 22-23 0.09832222028461501 1103 24-25 0.1860821014755345 1103 26-27 0.011277298181347817 1103 28-29 0.0027318745158027014 1103 30-31 0.005761672893079606 1103 32-33 0.05165908905038208 1103 34-35 0.14166482686349724 1103 36-37 0.20053060092288888 1103 38-39 0.03752308394506798 1103 40-41 0.18207486429930952 1103 42-43 0.06156685701715503 1103 44-45 0.053861298885898634 1103 46-47 0.17568798175101819 1103 48-49 0.26257224954633784 1103 50-51 -0.013379135482907145 1103 52-53 0.12982681544432495 1103 54-55 0.34416103709430246 1103 56-57 0.30876477997968266 1103 58-59 0.24051663831593117 1103 60-61 0.43761221815174167 1103 62-63 0.36264887614914443 1103 64-65 0.29020359276940866 1103 66-67 0.4277055210281375 1103 68-69 0.3574603128266034 1103 70-71 0.2750404742005692 1103 72-73 0.28544615619616565 1103 74-75 0.36478531789902746 1103 76-77 0.3946678950839946 1103 78-79 0.15496557130487432 1103 80-81 0.21199177404082548 1103 82-83 0.14605396669020365 1103 84-85 0.15724621486309331 1103 86-87 0.3327755689550038 1103 88-89 0.3801882656851596 1103 90-91 0.36680628205863997 1103 92-93 0.28037974654312237 1103 94-95 -0.02333447432437552 1103 96-97 0.04577748126435921 1103 98-99 0.12683575272657777 1103 100-101 0.7749776089269567 1104 1 0.005576926421941408 1104 2 0.0416752169628829 1104 3 0.00695179800540302 1104 4 0.05862442795974232 1104 5 -8.865911213504774E-4 1104 6 0.04956352865314528 1104 7 0.044385264469283925 1104 8 0.037742715640476376 1104 9 0.14481950694190715 1104 10-11 0.12384484317239242 1104 12-13 0.0508749657019294 1104 14-15 0.10182465602868973 1104 16-17 0.16691589175163557 1104 18-19 0.20463337874426912 1104 20-21 0.18060868471582125 1104 22-23 0.025314382389524326 1104 24-25 0.06585185874183708 1104 26-27 0.09463071931663336 1104 28-29 0.08501881598359518 1104 30-31 0.10740010706717129 1104 32-33 0.04421418441265246 1104 34-35 0.1611017840362976 1104 36-37 0.27126961289521745 1104 38-39 0.3224455450295167 1104 40-41 0.2164374235583253 1104 42-43 0.3779015271023951 1104 44-45 0.5477129705562547 1104 46-47 0.4864733052376735 1104 48-49 0.6950808873011809 1104 50-51 0.4253990878788372 1104 52-53 0.3829499207993621 1104 54-55 0.35721883883135064 1104 56-57 0.31305188214990665 1104 58-59 0.3666965227562784 1104 60-61 0.49049865267646453 1104 62-63 0.7145820531628644 1104 64-65 0.7482412701812251 1104 66-67 0.7121626755727348 1104 68-69 0.5739279276375271 1104 70-71 0.5561126514991592 1104 72-73 0.6995718985317545 1104 74-75 0.5197338566027412 1104 76-77 0.6442555472947511 1104 78-79 0.46466980581352857 1104 80-81 0.578135427391107 1104 82-83 0.7381495992368272 1104 84-85 0.7353665809724141 1104 86-87 0.7241334313206913 1104 88-89 0.4350265040944983 1104 90-91 0.780267300765864 1104 92-93 0.7891511765754835 1104 94-95 0.6841527973138355 1104 96-97 0.6747119512020525 1104 98-99 0.8314610604351813 1104 100-101 1.4693325713445056 1105 1 -0.049063398728403484 1105 2 -0.09413221383054449 1105 3 -0.0896804687398216 1105 4 0.008014477925371466 1105 5 -0.03197696749782608 1105 6 -0.07137088284730453 1105 7 -0.05950982676305472 1105 8 -0.25319648869095346 1105 9 -0.08702775564806586 1105 10-11 -0.16063415264891034 1105 12-13 -0.24497271314615432 1105 14-15 -0.28535737920960713 1105 16-17 -0.2561222354386743 1105 18-19 -0.3080192553340808 1105 20-21 -0.29225744286728883 1105 22-23 -0.21621952872177985 1105 24-25 -0.24180465210652358 1105 26-27 -0.09632050373468815 1105 28-29 -0.2052536425334992 1105 30-31 -0.24286452462501273 1105 32-33 -0.36341096050819743 1105 34-35 -0.2593320917516948 1105 36-37 -0.22558822470256246 1105 38-39 -0.17608142893529788 1105 40-41 -0.08548659761220989 1105 42-43 -0.18110877528566505 1105 44-45 -0.04622402496897138 1105 46-47 -0.14008366862454835 1105 48-49 -0.20995744801817295 1105 50-51 -0.40971418855611574 1105 52-53 -0.3010736827939411 1105 54-55 -0.2112676379102325 1105 56-57 -0.36348728348711745 1105 58-59 -0.26554002910229 1105 60-61 -0.19412383493472873 1105 62-63 -0.2732954555617013 1105 64-65 -0.41229555620250125 1105 66-67 -0.24526936369623797 1105 68-69 -0.31808533985667253 1105 70-71 -0.4492843400100526 1105 72-73 -0.39270607393951096 1105 74-75 -0.3276332376078983 1105 76-77 -0.45747514836928005 1105 78-79 -0.36830838900405993 1105 80-81 -0.3088444881554011 1105 82-83 -0.1921202796525847 1105 84-85 -0.27448188055815237 1105 86-87 -0.24660523627229836 1105 88-89 -0.17241366979585848 1105 90-91 0.10955662799602806 1105 92-93 0.16087860373398044 1105 94-95 0.08632657334645799 1105 96-97 0.12130545024714934 1105 98-99 0.23692386176427505 1105 100-101 0.5426137844730512 1106 1 -0.05450969037444864 1106 2 0.02323280501824243 1106 3 -0.025421986785168826 1106 4 0.10650443643970675 1106 5 -0.021818478822375198 1106 6 -0.06668923490722989 1106 7 -0.2678243724064515 1106 8 -0.22158978988665012 1106 9 -0.25007282777446704 1106 10-11 -0.6434573161904069 1106 12-13 -0.31865772759586974 1106 14-15 -0.3032519665219624 1106 16-17 -0.16898767891979816 1106 18-19 -0.35648370535881924 1106 20-21 -0.23177055148151737 1106 22-23 -0.32693508004119565 1106 24-25 -0.24078522899552723 1106 26-27 -0.4258203845932371 1106 28-29 -0.07320902253361794 1106 30-31 -0.06334249567670014 1106 32-33 -0.03751678866345998 1106 34-35 0.12109678691343362 1106 36-37 -0.12120290292900648 1106 38-39 -0.09868622758624923 1106 40-41 -0.20574904483866163 1106 42-43 -0.12239678599006254 1106 44-45 0.17008796677055216 1106 46-47 0.03900593796428353 1106 48-49 0.2976998339682453 1106 50-51 0.3864419722787318 1106 52-53 0.429387577905878 1106 54-55 0.3107652817884414 1106 56-57 0.16188740109353716 1106 58-59 0.14604250537235686 1106 60-61 0.14158514833047775 1106 62-63 0.17041051556329734 1106 64-65 0.27023769648123164 1106 66-67 0.30462004860038405 1106 68-69 0.13664106249926533 1106 70-71 0.21870464398833533 1106 72-73 0.2658837189987615 1106 74-75 0.5245931800225918 1106 76-77 0.6280088408530027 1106 78-79 0.5893707355812374 1106 80-81 0.4513023918661041 1106 82-83 0.5578836917290282 1106 84-85 0.37610221802986743 1106 86-87 0.5189848804863217 1106 88-89 0.6698163220780842 1106 90-91 0.7812409825854232 1106 92-93 0.8476485568716754 1106 94-95 0.7978577853510274 1106 96-97 0.8737958120094653 1106 98-99 0.9247179731218189 1106 100-101 1.182879317157525 1107 1 0.042965584755823016 1107 2 0.008357823445720669 1107 3 0.08845182183824818 1107 4 -0.019716744585437596 1107 5 0.04988813007280868 1107 6 0.07485463531204317 1107 7 0.05791801464111046 1107 8 0.07019443875510234 1107 9 0.09877537466500996 1107 10-11 -0.020265292244296518 1107 12-13 0.019588745544872666 1107 14-15 0.009988097060855239 1107 16-17 0.04631385873314997 1107 18-19 0.17367374543179182 1107 20-21 0.07207037610969991 1107 22-23 0.04986344191474501 1107 24-25 0.10847190324005851 1107 26-27 0.21937228671821885 1107 28-29 0.06456362403694982 1107 30-31 0.022726009678486037 1107 32-33 0.13714382524400293 1107 34-35 0.01684388498608058 1107 36-37 0.007414811807208821 1107 38-39 0.0857907845342325 1107 40-41 0.09563365704639182 1107 42-43 -0.05221949761764222 1107 44-45 -0.25182759371323726 1107 46-47 0.0744544788284216 1107 48-49 -0.049117660279172526 1107 50-51 -0.11673861060646828 1107 52-53 -0.15921991358543153 1107 54-55 -0.17446100765437933 1107 56-57 -0.23126014052051858 1107 58-59 -0.2619282606339155 1107 60-61 -0.1587140177860249 1107 62-63 -0.07071769482403312 1107 64-65 0.05913057353949824 1107 66-67 -0.04123104454972548 1107 68-69 -0.21785988932008138 1107 70-71 -0.315477368228283 1107 72-73 -0.22103982138466094 1107 74-75 -0.18778271491193266 1107 76-77 -0.21432404594779442 1107 78-79 -0.2044824983612159 1107 80-81 0.010504273712314216 1107 82-83 -0.028791478284496463 1107 84-85 -0.1349398478988455 1107 86-87 -0.0036267579889717183 1107 88-89 0.14244190134187917 1107 90-91 0.0793415100920356 1107 92-93 0.021284923421472257 1107 94-95 -0.04745186039270877 1107 96-97 0.1709368286935451 1107 98-99 0.4732501843552086 1107 100-101 1.0443091613411717 1108 1 0.030986120979441978 1108 2 -0.015030653451027831 1108 3 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72-73 -1.4171723634955278 2316 74-75 -1.2703992807002038 2316 76-77 -1.2582656212641545 2316 78-79 -1.0568036892448731 2316 80-81 -1.0304817416034204 2316 82-83 -1.0808844065896324 2316 84-85 -0.9401704606023635 2316 86-87 -0.9239484658178121 2316 88-89 -1.0066980187620977 2316 90-91 -0.7381949497718594 2316 92-93 -0.7512501411843253 2316 94-95 -0.7073171583070597 2316 96-97 -0.9534728618884216 2316 98-99 -0.7248377769957273 2316 100-101 -0.8352360256769202 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5359.0 3 1131.0 4 716.0 5 836.0 6 1071.0 7 1199.0 8 1627.0 9 2056.0 10 2843.0 11 4191.0 12 6385.0 13 9283.0 14 10345.0 15 14330.0 16 20068.0 17 28653.0 18 22569.0 19 11884.0 20 10934.0 21 11014.0 22 11520.0 23 12837.0 24 14145.0 25 16164.0 26 18726.0 27 21928.0 28 25912.0 29 31117.0 30 37711.0 31 47182.0 32 60115.0 33 79153.0 34 107857.0 35 154998.0 36 224797.0 37 323764.0 38 274430.0 39 34060.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.15971727444463 19.347146523736676 15.116691199606509 20.376445002212186 2 14.900525364917097 21.273301844381585 41.190043738226294 22.636129052475024 3 18.741055638073835 27.638146140705544 30.775715322991264 22.845082898229354 4 11.675319001491435 17.12589825095286 38.75635366606909 32.442429081486615 5 12.170090450565542 39.30996040904626 33.675571116079226 14.844378024308966 6 29.015583080125108 37.496195095606325 18.36142884783577 15.126792976432798 7 26.885830877078774 35.251020174439276 20.81975612255354 17.043392825928404 8 26.73827603519073 33.392800208776954 20.177713542837548 19.691210213194775 9 27.855031324299155 16.324675833431268 20.897209892391466 34.92308294987811 10-11 24.153936562947337 27.692187146839448 27.121193400135613 21.0326828900776 12-13 26.442940560974787 25.66945450337289 27.852489765609555 20.03511517004277 14-15 23.180347301885185 25.773766531100268 28.237706140624447 22.808180026390097 16-17 20.298248738712758 29.573716404223088 28.450830968372664 21.677203888691494 18-19 20.210013852464773 27.2409910011713 32.70377994198056 19.845215204383372 20-21 21.89324276195303 25.853972933443654 32.386851561807916 19.8659327427954 22-23 21.227210738410342 26.193375995622485 32.348924815097604 20.230488450869565 24-25 20.781264123314994 26.249209094360314 32.839185532904686 20.130341249420002 26-27 20.665310309001256 26.353123459483434 32.70939273665649 20.272173494858816 28-29 20.163770210601307 26.606933413052676 32.96405879140815 20.265237584937864 30-31 20.35848003548818 26.461024480093347 32.896300743033756 20.284194741384713 32-33 20.175105783408977 26.64376764626378 32.395986302777565 20.78514026754968 34-35 20.772061205773383 26.357384888065383 32.453098247677175 20.41745565848406 36-37 20.9181708777345 26.08824169261485 32.32457332392429 20.669014105726358 38-39 20.727694345957033 26.339071432510014 32.41001069358876 20.523223527944197 40-41 20.591910270389814 26.51697871826292 32.44409600750117 20.447015003846097 42-43 20.415924926448696 26.75821056704106 31.824150619566517 21.001713886943723 44-45 20.548301888498287 26.96621256115808 31.45720102041893 21.028284529924704 46-47 20.97830126044304 27.15867031893672 30.64600757236253 21.217020848257704 48-49 21.08491016697162 27.41187508457683 30.0893703987566 21.41384434969495 50-51 21.258711246387488 27.707633045517742 29.902401490545007 21.13125421754977 52-53 21.258466797493195 27.87213967691273 29.677153889979106 21.19223963561496 54-55 20.7473676850336 27.67661887069047 29.85920400049186 21.716809443784072 56-57 21.203088306101947 28.121013293190817 29.285304934672997 21.39059346603424 58-59 21.385568483696023 27.51958652444708 29.41807702295674 21.676767968900158 60-61 21.178621888433995 27.54481890181264 30.103540625461296 21.173018584292073 62-63 21.371122994652406 27.482172177449726 30.038713564811324 21.10799126308654 64-65 21.161172970030464 27.685977675358515 29.982819551669525 21.170029802941492 66-67 20.941428108783903 27.822167995963156 29.619015341512462 21.617388553740483 68-69 21.02096076815178 27.85863712830005 29.70731566237184 21.41308644117633 70-71 21.008546653206043 27.885305113018937 29.512660910652844 21.59348732312218 72-73 20.96193023545879 27.81694023478996 29.332084446294942 21.889045083456303 74-75 20.837518656952497 28.30243876572773 29.239790572130474 21.6202520051893 76-77 20.942913205509566 27.81995916517614 29.397276553483014 21.83985107583128 78-79 20.83997705280386 27.815490803646004 29.476691843501087 21.867840300049053 80-81 20.85565106308619 28.30285829635053 29.17670700880658 21.664783631756706 82-83 20.895557461608057 28.099893620578857 29.1461236284933 21.85842528931979 84-85 20.833963507920316 27.84372433482185 29.013709987930774 22.308602169327056 86-87 21.021317162046408 27.798391608960493 29.058029767677855 22.122261461315247 88-89 21.175826894043134 27.340281650591415 29.09998773755475 22.383903717810703 90-91 20.794860267592952 27.43493677782023 29.447458276736093 22.32274467785072 92-93 20.855408586383398 27.519184945252366 29.491563926668935 22.1338425416953 94-95 20.633208132217682 27.33537941300792 29.5029846110184 22.528427843755995 96-97 20.633564275112583 27.302392317394393 29.306566022090625 22.7574773854024 98-99 20.61180767109914 27.404819371498995 29.032539947991527 22.95083300941034 100-101 20.752085821834136 26.914881598826305 29.79895675016983 22.534075829169723 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2700.0 1 1659.5 2 682.0 3 779.0 4 691.5 5 673.0 6 1144.0 7 2434.0 8 4078.0 9 4771.5 10 4327.0 11 3846.5 12 3863.5 13 4120.0 14 4379.5 15 4468.5 16 4561.5 17 4732.0 18 4914.0 19 5178.0 20 5540.5 21 6073.0 22 6773.5 23 7785.5 24 8973.0 25 10503.0 26 12791.5 27 15171.5 28 16816.5 29 19101.5 30 22602.5 31 25791.0 32 29738.5 33 33964.0 34 37941.5 35 41377.5 36 45877.5 37 50311.5 38 51612.5 39 51944.5 40 52746.5 41 54438.5 42 55908.5 43 57941.0 44 60712.5 45 62464.5 46 61739.5 47 63153.0 48 73927.0 49 72406.5 50 58846.5 51 51241.5 52 45725.5 53 43337.5 54 41696.0 55 38045.0 56 33640.0 57 29753.0 58 27253.5 59 24829.5 60 21225.0 61 17751.0 62 14383.5 63 10401.5 64 6986.5 65 5211.0 66 3702.5 67 2669.5 68 1979.5 69 1368.5 70 986.0 71 717.0 72 552.0 73 479.0 74 377.5 75 282.0 76 221.0 77 150.5 78 97.0 79 61.0 80 37.5 81 28.0 82 18.0 83 14.5 84 15.0 85 10.5 86 9.5 87 7.5 88 2.0 89 1.0 90 4.0 91 5.0 92 2.5 93 2.5 94 2.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2355507901505252 2 0.21005333418324854 3 0.2012134143078012 4 0.2066256101499118 5 0.20692628769669574 6 0.23260415019204275 7 0.23338591181368096 8 0.22394463684466576 9 0.2198554222084044 10-11 0.22767303842478642 12-13 0.22845480004642463 14-15 0.21507464921454006 16-17 0.2280639192356055 18-19 0.2188631863040175 20-21 0.2116769929358817 22-23 0.21179726395459528 24-25 0.20572357750956002 26-27 0.21438309085693702 28-29 0.22592910865343965 30-31 0.2265304637470075 32-33 0.2250571437677663 34-35 0.2335963860964297 36-37 0.21131617987974102 38-39 0.21080502805020834 40-41 0.20665567790459022 42-43 0.21197767048266566 44-45 0.22782337719817838 46-47 0.2316119142876558 48-49 0.2355207223958468 50-51 0.23576126443327394 52-53 0.25413266254177164 54-55 0.23543051913181165 56-57 0.20987292765517815 58-59 0.19387688216627347 60-61 0.1909903777171478 62-63 0.19760528374639413 64-65 0.19096030996246943 66-67 0.19234342667767546 68-69 0.19477891480662526 70-71 0.1957711507110122 72-73 0.1984471808773891 74-75 0.20097287227037408 76-77 0.21306010965108776 78-79 0.20740737177155 80-81 0.20181476940136903 82-83 0.19757521599171576 84-85 0.19952962004581126 86-87 0.20605432281102237 88-89 0.202807005305756 90-91 0.21197767048266566 92-93 0.20094280451569568 94-95 0.19931914576306248 96-97 0.2089107595054696 98-99 0.21444322636629382 100-101 0.19204274913089156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1662911.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.119968507514905 #Duplication Level Percentage of deduplicated Percentage of total 1 71.54217323479118 31.56438430078163 2 14.305563211725497 12.623219967671853 3 5.3381098785862005 7.06551719198632 4 2.6990250860908223 4.763236071972791 5 1.5264420916767658 3.3673288506662042 6 0.9663713501952454 2.5581764122307336 7 0.6399855970403062 1.9765301070677002 8 0.46709476481493994 1.6486565050928184 9 0.3273556221885077 1.2998627767543416 >10 1.9283494595119923 15.879804115134858 >50 0.16226133533113182 4.912633238818658 >100 0.09018455655547479 7.157515878781494 >500 0.004359268610459529 1.3293664054604664 >1k 0.0023158614493066247 1.8010576772777076 >5k 1.3622714407686027E-4 0.466522381733617 >10k+ 2.7245428815372055E-4 1.5861881185689108 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15171 0.9123158124517787 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11220 0.674720414983123 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7762 0.4667718236273619 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3241 0.19489918582533883 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2659 0.1599003193796902 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2634 0.15839693164577057 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2495 0.15003809584517752 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2319 0.13945424619838342 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2070 0.12448050436854408 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1806 0.10860472989835294 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1757 0.10565808993987051 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.013550935678458E-5 0.0 8 0.0 0.0 0.0 6.013550935678458E-5 0.0 9 0.0 0.0 0.0 2.4054203742713832E-4 0.0 10-11 0.0 0.0 0.0 2.4054203742713832E-4 0.0 12-13 0.0 0.0 0.0 2.4054203742713832E-4 0.0 14-15 0.0 0.0 0.0 2.4054203742713832E-4 0.0 16-17 0.0 0.0 0.0 2.4054203742713832E-4 0.0 18-19 0.0 0.0 0.0 2.706097921055306E-4 0.0 20-21 0.0 0.0 0.0 3.006775467839229E-4 0.0 22-23 0.0 0.0 0.0 4.5101632017588433E-4 0.0 24-25 0.0 0.0 0.0 7.817616216381994E-4 0.0 26-27 0.0 0.0 0.0 0.001353048960527653 0.0 28-29 0.0 0.0 0.0 0.0022550816008794217 0.0 30-31 0.0 0.0 0.0 0.004029079126904566 0.0 32-33 0.0 0.0 0.0 0.006193957463748812 0.0 34-35 0.0 0.0 0.0 0.009260868440944824 0.0 36-37 0.0 0.0 0.0 0.012267643908784053 0.0 38-39 0.0 0.0 0.0 0.015965977734226304 0.0 40-41 0.0 0.0 0.0 0.024505220062889715 0.0 42-43 0.0 0.0 0.0 0.03325493667430187 0.0 44-45 0.0 0.0 0.0 0.05303951925268399 0.0 46-47 0.0 0.0 0.0 0.0951343758024332 0.0 48-49 0.0 0.0 0.0 0.13157649447264466 0.0 50-51 0.0 0.0 0.0 0.17676832975426826 0.0 52-53 0.0 0.0 0.0 0.2324538114186508 0.0 54-55 0.0 0.0 0.0 0.2996552431248576 0.0 56-57 0.0 0.0 0.0 0.3732009710682051 0.0 58-59 0.0 0.0 0.0 0.48415098583147265 0.0 60-61 0.0 0.0 0.0 0.6195761529029515 0.0 62-63 0.0 0.0 0.0 0.7679304544861391 0.0 64-65 0.0 0.0 0.0 0.8974022061312963 0.0 66-67 0.0 0.0 0.0 1.0837320818732934 0.0 68-69 0.0 0.0 0.0 1.3117057978448636 0.0 70-71 0.0 0.0 0.0 1.5094614203646497 0.0 72-73 0.0 0.0 0.0 1.8098683573564671 0.0 74-75 0.0 0.0 0.0 2.068300708817249 0.0 76-77 0.0 0.0 0.0 2.3113383698826935 0.0 78-79 0.0 0.0 0.0 2.6177889255648683 0.0 80-81 0.0 0.0 0.0 2.934342246818982 0.0 82-83 0.0 0.0 0.0 3.293050560132202 0.0 84-85 0.0 0.0 0.0 3.7732025345914484 0.0 86-87 0.0 0.0 0.0 4.3513152537928965 0.0 88-89 0.0 0.0 0.0 5.014549786488875 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13820 0.0 27.957506 1 GTATCAA 19455 0.0 27.847715 1 GTACATG 13765 0.0 26.204838 1 TACATGG 13805 0.0 25.639996 2 ACATGGG 13890 0.0 24.320103 3 CATGGGA 9830 0.0 23.055578 4 ATCAACG 23530 0.0 23.018711 3 GAGTACT 11620 0.0 22.994438 12-13 TCAACGC 23640 0.0 22.892195 4 TATCAAC 23745 0.0 22.830294 2 CAACGCA 23890 0.0 22.592289 5 AGTACTT 11780 0.0 22.35953 12-13 AACGCAG 24315 0.0 22.215601 6 GTACTTT 12600 0.0 21.129627 14-15 TACCTGG 1135 0.0 20.930145 2 ATGGGAG 3660 0.0 20.770021 5 ATGGGAT 3860 0.0 20.678549 5 CGCAGAG 26310 0.0 20.277655 8 ACGCAGA 26420 0.0 20.247171 7 GCAGAGT 26910 0.0 19.507172 9 >>END_MODULE