FastQCFastQC Report
Thu 26 May 2016
SRR937412_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937412_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662911
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145570.8753926097067131No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118110.7102605010129827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85190.5122944042104478No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA34780.20915130154289677No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33630.2022357179668665No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29520.1775200236212281No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26700.16056180998261482No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20560.12363860723754909No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT17330.10421483771530766No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16930.10180941734103628No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA211700.025.8447991
GTACATG143300.025.0449091
TACATGG143450.024.4362682
GGTATCA154900.024.028631
CGACCGA601.7121549E-423.74920346-47
GAGTACT116650.023.33253912-13
ACATGGG144050.023.1804083
GGACCGT1452.3357605E-522.9302656
AGTACTT118750.022.37992112-13
TAAGGTG5900.021.736565
GTACTTT124550.021.71846214-15
ATCAACG253400.021.4436383
GTATAGG5100.021.4376091
TCAACGC254450.021.355154
CAACGCA256750.021.1638475
TATCAAC257650.021.163662
CATGGGG38850.021.0288944
AACGCAG260150.020.9055086
CATGGGA104850.020.612094
TACTTTT135900.019.46770714-15
Produced by FastQC (version 0.11.5)