##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937412_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1662911 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.254827829029935 33.0 31.0 34.0 31.0 34.0 2 32.43817738892821 34.0 31.0 34.0 31.0 34.0 3 32.47784818309579 34.0 31.0 34.0 31.0 34.0 4 36.12087477922751 37.0 35.0 37.0 35.0 37.0 5 36.06072122921792 37.0 35.0 37.0 35.0 37.0 6 36.12025598483623 37.0 35.0 37.0 35.0 37.0 7 36.064865167167696 37.0 35.0 37.0 35.0 37.0 8 36.05028651563433 37.0 35.0 37.0 35.0 37.0 9 37.747030959564285 39.0 37.0 39.0 35.0 39.0 10-11 37.6742504559775 39.0 37.0 39.0 35.0 39.0 12-13 37.58341667112671 39.0 37.0 39.0 35.0 39.0 14-15 38.7315190049257 40.0 38.0 41.0 34.5 41.0 16-17 38.72964458109905 40.0 38.0 41.0 34.5 41.0 18-19 38.79934193712111 40.0 38.0 41.0 35.0 41.0 20-21 38.744010352929294 40.0 38.0 41.0 34.5 41.0 22-23 38.60535260155234 40.0 38.0 41.0 34.0 41.0 24-25 38.53066219418839 40.0 38.0 41.0 34.0 41.0 26-27 38.45185851798443 40.0 38.0 41.0 34.0 41.0 28-29 38.31881171030801 40.0 38.0 41.0 34.0 41.0 30-31 38.309734856525694 40.0 38.0 41.0 34.0 41.0 32-33 38.17989447420818 40.0 37.0 41.0 34.0 41.0 34-35 38.019332964903114 40.0 37.0 41.0 34.0 41.0 36-37 37.82661308993687 40.0 37.0 41.0 33.0 41.0 38-39 37.67589726690123 40.0 37.0 41.0 33.0 41.0 40-41 37.46630938156041 40.0 36.5 41.0 33.0 41.0 42-43 37.19017854834083 40.0 36.0 41.0 32.5 41.0 44-45 36.78724748347928 40.0 36.0 41.0 31.5 41.0 46-47 36.26278977046877 40.0 36.0 41.0 30.0 41.0 48-49 35.799534972106144 39.0 35.0 41.0 28.5 41.0 50-51 35.51459939828409 39.0 35.0 41.0 27.0 41.0 52-53 35.26054972274523 39.0 35.0 41.0 26.5 41.0 54-55 35.039365305780045 39.0 35.0 40.0 26.0 41.0 56-57 34.78570440630918 38.5 35.0 40.0 25.5 41.0 58-59 34.5350223192943 38.0 34.0 40.0 25.0 41.0 60-61 34.21875464170963 38.0 34.0 40.0 23.5 41.0 62-63 33.87303499706238 37.5 34.0 40.0 22.5 41.0 64-65 33.484374990603825 37.0 33.5 40.0 22.0 41.0 66-67 33.077778967124516 36.0 33.0 39.0 20.0 41.0 68-69 32.65471303034258 36.0 32.5 39.0 18.5 40.0 70-71 32.250012778795735 35.0 32.0 38.5 17.5 40.0 72-73 31.802856256287917 35.0 32.0 38.0 13.5 40.0 74-75 31.337874967451654 35.0 31.0 37.0 9.0 39.0 76-77 30.047358517683747 34.0 29.5 35.5 7.0 38.5 78-79 30.620515469559106 34.5 31.0 36.0 6.5 38.5 80-81 30.42433449535183 34.5 31.0 36.0 2.0 37.5 82-83 30.087338709046968 34.0 31.0 35.0 2.0 37.0 84-85 29.745664981469243 34.0 30.5 35.0 2.0 36.5 86-87 29.350746371874383 34.0 30.0 35.0 2.0 36.0 88-89 28.908134289808658 34.0 29.0 35.0 2.0 36.0 90-91 28.43450701811462 34.0 29.0 35.0 2.0 35.0 92-93 28.146484387919738 34.0 29.0 35.0 2.0 35.0 94-95 27.775155134580263 34.0 28.5 35.0 2.0 35.0 96-97 27.366024399381566 33.5 27.0 35.0 2.0 35.0 98-99 26.725813347797928 33.0 26.0 35.0 2.0 35.0 100-101 24.440282432433246 31.0 20.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04426909029019299 1101 2 -0.04087158645048561 1101 3 0.015145876162520722 1101 4 6.673738849940491E-4 1101 5 0.04584906079170992 1101 6 -0.038249152587873425 1101 7 -0.0877486418875364 1101 8 -0.06689956367262795 1101 9 -0.0020120375876615526 1101 10-11 -0.11220946591169678 1101 12-13 -0.1001875454844594 1101 14-15 -0.23593057008720564 1101 16-17 -0.10705302984209197 1101 18-19 0.009333169244612805 1101 20-21 -0.05710148240358848 1101 22-23 -0.08373079648948334 1101 24-25 -0.10688334280661849 1101 26-27 -0.10969005223862638 1101 28-29 -0.17993056811597086 1101 30-31 -0.06693335505151765 1101 32-33 0.023819745629928946 1101 34-35 -0.02469080979028604 1101 36-37 -0.12283909184204589 1101 38-39 -0.08808355653470556 1101 40-41 -0.14092039498046205 1101 42-43 -0.0436372891386938 1101 44-45 0.025828819433804995 1101 46-47 0.005482796472982443 1101 48-49 -0.015999190622494552 1101 50-51 0.056516028015963116 1101 52-53 0.10352016923038576 1101 54-55 -0.00787234331536979 1101 56-57 0.1882342599339637 1101 58-59 0.14313539919695728 1101 60-61 0.12762357311519423 1101 62-63 0.2514618321014481 1101 64-65 0.17080635765321972 1101 66-67 0.04982027861756677 1101 68-69 -0.061037895963430344 1101 70-71 -0.029836529619103658 1101 72-73 -0.11680625139271328 1101 74-75 -0.17057015810275544 1101 76-77 0.02218829492389318 1101 78-79 -0.2074485356669662 1101 80-81 -0.02474484385086484 1101 82-83 0.07691120087049086 1101 84-85 0.11946045619827217 1101 86-87 -0.04201091117900191 1101 88-89 -0.033664803448047564 1101 90-91 -0.09557514610611761 1101 92-93 -0.2174912181504105 1101 94-95 -0.15536670255122687 1101 96-97 -0.24136785458460608 1101 98-99 -0.2235544315300011 1101 100-101 0.32255276158991464 1102 1 -0.08699139530758515 1102 2 -0.08359389146787066 1102 3 -0.12792416854686905 1102 4 -0.06291931265251094 1102 5 -0.057479304831737466 1102 6 -0.18877076212587696 1102 7 -0.16921908401371155 1102 8 -0.09769936496422815 1102 9 -0.08695987663386262 1102 10-11 -0.0856322180229725 1102 12-13 -0.13918406808753758 1102 14-15 0.06014493910306129 1102 16-17 -0.05315337758177918 1102 18-19 -0.053259975315747 1102 20-21 -2.2120421183302597E-4 1102 22-23 -0.04771489981785493 1102 24-25 -0.03758378990050204 1102 26-27 0.010528526996345988 1102 28-29 -0.13174378222426952 1102 30-31 -0.030669072885586957 1102 32-33 0.03797771880429224 1102 34-35 -0.06492925986479747 1102 36-37 -0.016778485781436814 1102 38-39 0.10242811758352843 1102 40-41 0.09156842767229278 1102 42-43 0.048265343747544875 1102 44-45 0.029802987342399945 1102 46-47 -0.01587835603570653 1102 48-49 0.24207333794183228 1102 50-51 0.25025671355992785 1102 52-53 0.1949260311280412 1102 54-55 0.2842289979663022 1102 56-57 0.08813490573623994 1102 58-59 0.16077076929133938 1102 60-61 0.11570106938941649 1102 62-63 0.2815164769101912 1102 64-65 0.13801947240732204 1102 66-67 0.2251804375842923 1102 68-69 0.2109442202809788 1102 70-71 0.28139049472267885 1102 72-73 0.30818132933257303 1102 74-75 0.08700559947300235 1102 76-77 0.23381273605653163 1102 78-79 0.2016423734239403 1102 80-81 0.06194169365534563 1102 82-83 0.3012033022117713 1102 84-85 0.19894381437015696 1102 86-87 0.16092003765358598 1102 88-89 0.41790002516596303 1102 90-91 0.3075545111219 1102 92-93 0.32324400291268063 1102 94-95 0.31333672516859323 1102 96-97 0.47249205599661437 1102 98-99 0.25210379002986016 1102 100-101 0.7537499796723743 1103 1 0.03443962824259472 1103 2 0.08036476749278876 1103 3 -0.0160432643428976 1103 4 0.07787447084419341 1103 5 0.09188882670353138 1103 6 -0.024913880636049157 1103 7 -0.023613487868331617 1103 8 -0.031670826884514724 1103 9 -0.09603988099575389 1103 10-11 -0.07333491472207498 1103 12-13 0.057964547213813944 1103 14-15 -0.06050175171557015 1103 16-17 -0.04423088597236813 1103 18-19 -0.028699294537553044 1103 20-21 -0.030042003164979292 1103 22-23 0.0924718537635485 1103 24-25 0.026622850675586562 1103 26-27 -0.05099231921062142 1103 28-29 -0.10886081419935323 1103 30-31 -0.1071217241483211 1103 32-33 -0.0648619534076289 1103 34-35 -0.07839740795909478 1103 36-37 -0.0843086454347528 1103 38-39 0.03798843890954373 1103 40-41 -0.0872868988839528 1103 42-43 0.003443272576014067 1103 44-45 -0.29553919901537995 1103 46-47 0.045175202338917586 1103 48-49 -0.0840770099997954 1103 50-51 -0.07694614267131783 1103 52-53 -0.2573411428104535 1103 54-55 -0.282261173641821 1103 56-57 -0.17697198080206533 1103 58-59 -0.21673317862444463 1103 60-61 -0.1263059661801904 1103 62-63 -0.13804297203750338 1103 64-65 -0.5512615828473457 1103 66-67 -0.2019250838435056 1103 68-69 -0.4003680224986752 1103 70-71 -0.4202318866278141 1103 72-73 -0.21443305925543044 1103 74-75 -0.15318075022953792 1103 76-77 -0.021794919870995244 1103 78-79 -0.12871154561450382 1103 80-81 -0.29571443581205514 1103 82-83 -0.27225256796540975 1103 84-85 -0.18405652084929258 1103 86-87 -0.3878111769312902 1103 88-89 -0.2431183957484997 1103 90-91 -0.1715209294089597 1103 92-93 -0.005733093117594734 1103 94-95 -0.17917414926951025 1103 96-97 -0.0547402833908599 1103 98-99 0.09883014999092765 1103 100-101 0.6992178531290527 1104 1 -0.03554627377275921 1104 2 0.007522745272027009 1104 3 -0.005043914457210974 1104 4 -0.0741412487739197 1104 5 -0.07982462628005749 1104 6 -0.0717834706257463 1104 7 -0.021178115805717823 1104 8 -0.039882720324200704 1104 9 -0.11462292396904417 1104 10-11 -0.11538197131124406 1104 12-13 -0.11752833439963695 1104 14-15 -0.06925345917873216 1104 16-17 -0.1005125242604521 1104 18-19 -0.1490895092213691 1104 20-21 -0.14473750861059642 1104 22-23 -0.2370075434563077 1104 24-25 -0.08753284074655454 1104 26-27 -0.3402895383283706 1104 28-29 -0.20661024991473909 1104 30-31 -0.12816205314999252 1104 32-33 -0.0837678621432687 1104 34-35 -0.05885526375059413 1104 36-37 -0.11329278702819323 1104 38-39 -0.20192565834463494 1104 40-41 -0.16420784060010618 1104 42-43 -0.19988984161766865 1104 44-45 0.10751926014064139 1104 46-47 0.020361288431587354 1104 48-49 0.15482174017368777 1104 50-51 0.18065106402850972 1104 52-53 0.14625639990351402 1104 54-55 0.19463347131541298 1104 56-57 0.2805514488796703 1104 58-59 0.06569255129323182 1104 60-61 0.3244789190271504 1104 62-63 0.34076184026937284 1104 64-65 0.08908056720488133 1104 66-67 0.2138836510632487 1104 68-69 0.3643884768183412 1104 70-71 0.2365055621482881 1104 72-73 0.3735281384158462 1104 74-75 0.3142879675843204 1104 76-77 0.3926171649506607 1104 78-79 0.12281914489455659 1104 80-81 0.14638740569153086 1104 82-83 0.24838235266950903 1104 84-85 0.21176813820592244 1104 86-87 0.20474099656888356 1104 88-89 0.3658319983376508 1104 90-91 0.25232000441816993 1104 92-93 0.33980120589116325 1104 94-95 0.3989359903224532 1104 96-97 0.3219096556152685 1104 98-99 0.3044693438491741 1104 100-101 0.8392984965230887 1105 1 -0.08318173720434174 1105 2 -0.0816400336318992 1105 3 -0.09067201715571116 1105 4 -0.07373319857671135 1105 5 -0.1990752444379993 1105 6 -0.1453239656504408 1105 7 -0.1555931339290666 1105 8 -0.14904992563471353 1105 9 -0.09882241126803848 1105 10-11 -0.17687032854276907 1105 12-13 -0.15795520415335318 1105 14-15 -0.2195334806951692 1105 16-17 -0.20918930717158446 1105 18-19 -0.24066541256740948 1105 20-21 -0.2791686494069765 1105 22-23 -0.30431413367218596 1105 24-25 -0.28906214956756315 1105 26-27 -0.11489237093544347 1105 28-29 -0.38243606055540624 1105 30-31 -0.354420317852032 1105 32-33 -0.3426529939607761 1105 34-35 -0.3600452635893632 1105 36-37 -0.3671474982931002 1105 38-39 -0.5369303265913246 1105 40-41 -0.6053874926141916 1105 42-43 -0.553919557169742 1105 44-45 -0.5620449440075745 1105 46-47 -0.6833088182272249 1105 48-49 -0.5633561339492701 1105 50-51 -0.762376099744138 1105 52-53 -0.5842057785041206 1105 54-55 -0.4843186721939645 1105 56-57 -0.44071508968502826 1105 58-59 -0.4449223234848958 1105 60-61 -0.492822321392687 1105 62-63 -0.45920107758266937 1105 64-65 -0.4906312473433374 1105 66-67 -0.3641301528762497 1105 68-69 -0.34684653459211745 1105 70-71 -0.45179729200454233 1105 72-73 -0.4868641701897012 1105 74-75 -0.3540468094610958 1105 76-77 -0.39854350628018764 1105 78-79 -0.3886645953897023 1105 80-81 -0.46790549051216246 1105 82-83 -0.5338440125911106 1105 84-85 -0.42470515898330774 1105 86-87 -0.47052491760866033 1105 88-89 -0.3556044145727988 1105 90-91 -0.23749970012651644 1105 92-93 -0.18331856065253405 1105 94-95 -0.038791650827885604 1105 96-97 0.057814701865350315 1105 98-99 -0.016110808554056888 1105 100-101 0.40802168341857836 1106 1 -0.027158678751710852 1106 2 0.028843802844939148 1106 3 -0.016113298751101013 1106 4 -8.167992145757808E-4 1106 5 -0.03567580287535321 1106 6 0.044260322341934 1106 7 0.007361512892913424 1106 8 -0.022392790283923603 1106 9 -0.04150895928204079 1106 10-11 -0.1820526760569905 1106 12-13 -0.14484139657595563 1106 14-15 -0.01287607040256944 1106 16-17 0.030131953161642855 1106 18-19 -0.0029380509700587254 1106 20-21 0.043379938919606786 1106 22-23 0.03556052341428284 1106 24-25 0.0403488334410369 1106 26-27 0.005440950178247306 1106 28-29 -0.06638113940260126 1106 30-31 -0.03594545058783183 1106 32-33 0.04269871770470246 1106 34-35 0.19077146631391173 1106 36-37 0.058515111454504165 1106 38-39 -0.13658221971839168 1106 40-41 -0.06482945239937266 1106 42-43 -0.17271457889014386 1106 44-45 -0.1416781716410611 1106 46-47 -0.32381706342643923 1106 48-49 -0.2793139089979988 1106 50-51 0.003964913630255751 1106 52-53 0.04567718793464337 1106 54-55 -0.08291127564845624 1106 56-57 -0.14345442652540186 1106 58-59 -0.12423218900780597 1106 60-61 -0.02874346808329875 1106 62-63 0.11620476706909955 1106 64-65 0.10861174622301917 1106 66-67 0.053909848101319824 1106 68-69 0.1925805783356509 1106 70-71 0.1346210411149471 1106 72-73 0.023717423372993096 1106 74-75 0.13277202221953388 1106 76-77 0.2331074887036486 1106 78-79 0.32208547640066243 1106 80-81 0.31473896085674014 1106 82-83 0.10046903003708607 1106 84-85 0.1585525653539257 1106 86-87 0.30280801858651074 1106 88-89 0.4483594825134283 1106 90-91 0.4020958910289103 1106 92-93 0.47514903592841407 1106 94-95 0.40857135178100634 1106 96-97 0.39500317752689185 1106 98-99 0.16877701518991373 1106 100-101 0.9220985510371555 1107 1 0.02016661272293163 1107 2 -0.05305111886231373 1107 3 0.012641901002773182 1107 4 -0.0058104858000120885 1107 5 -0.05068858994342662 1107 6 -0.0028508898977577246 1107 7 -0.09181774321379521 1107 8 0.09619236389799113 1107 9 0.09308630918229 1107 10-11 0.02145976169017416 1107 12-13 -0.054607319520250996 1107 14-15 0.030745618259565788 1107 16-17 0.01766440154867155 1107 18-19 0.08395296090684923 1107 20-21 -0.023884846328151355 1107 22-23 0.01922336143834258 1107 24-25 -0.07806566626091893 1107 26-27 -0.07087461158744901 1107 28-29 0.06535017833841295 1107 30-31 0.047855032692837085 1107 32-33 0.04429277476258875 1107 34-35 0.011787285102890621 1107 36-37 0.053365832850246875 1107 38-39 -0.031450246920556424 1107 40-41 0.1049166329631177 1107 42-43 0.0850079769851746 1107 44-45 0.23378443820273986 1107 46-47 0.11755070418863056 1107 48-49 0.028275488311265917 1107 50-51 0.01817426261145272 1107 52-53 0.017846727151528796 1107 54-55 0.040737108822462176 1107 56-57 0.12440003805217259 1107 58-59 -0.10348589212990333 1107 60-61 -0.006337255939754982 1107 62-63 -0.09222604495779052 1107 64-65 0.04447194094539242 1107 66-67 0.0210116703386376 1107 68-69 -0.009115550932321526 1107 70-71 0.06089686362147262 1107 72-73 0.1958089262220426 1107 74-75 0.19250028901097238 1107 76-77 0.1778304996575848 1107 78-79 0.1258105512322487 1107 80-81 0.14033232568236897 1107 82-83 0.06239914021738713 1107 84-85 0.18009428837128993 1107 86-87 0.23700605469796088 1107 88-89 0.2117889485331581 1107 90-91 0.2774412059625391 1107 92-93 0.3870858761445426 1107 94-95 0.40877357333378583 1107 96-97 0.4877538072659604 1107 98-99 0.46252753596649754 1107 100-101 1.265780169065959 1108 1 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-0.2094659706210109 2316 70-71 -0.07227496550016355 2316 72-73 0.2370011433982846 2316 74-75 0.1723674143430145 2316 76-77 0.11181997471950567 2316 78-79 0.06493572852057383 2316 80-81 -0.19772759880082802 2316 82-83 0.0649768692640258 2316 84-85 -0.00533551285999323 2316 86-87 -0.1781853014517516 2316 88-89 -0.2300785900257658 2316 90-91 -0.16836392381292953 2316 92-93 -0.14901961689192333 2316 94-95 0.10563914042182887 2316 96-97 0.22637751480010948 2316 98-99 0.4252757910707139 2316 100-101 0.18136334990869685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 14.0 5 43.0 6 82.0 7 203.0 8 430.0 9 847.0 10 1613.0 11 2868.0 12 4534.0 13 6616.0 14 8283.0 15 12108.0 16 17866.0 17 26553.0 18 23132.0 19 11404.0 20 8838.0 21 8935.0 22 8829.0 23 9450.0 24 10570.0 25 12177.0 26 14553.0 27 17981.0 28 22241.0 29 27476.0 30 34298.0 31 42921.0 32 55527.0 33 72289.0 34 101137.0 35 147257.0 36 227704.0 37 361191.0 38 329010.0 39 33923.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.09893631426121 20.217671927422632 15.542389456421985 20.141002301894172 2 15.294985720823304 22.157890590656987 39.98464139091028 22.56248229760943 3 19.113229751922983 27.585180445616153 30.794792986515812 22.50679681594505 4 12.17533590192139 17.77509439771581 37.91519810741525 32.134371592947545 5 12.253326910737341 39.954380324725975 33.19147093685109 14.600821827685598 6 29.393515347484023 37.80400755061456 17.72295691110348 15.079520190797943 7 27.093993605189937 35.4706295165526 20.415825020100296 17.019551858157172 8 27.267183872137473 33.79934344050884 19.692635384575603 19.24083730277808 9 28.044074517517775 16.367622801220268 20.52623381527935 35.0620688659826 10-11 24.54427752811489 28.03620041746121 26.555000905040504 20.8645211493834 12-13 26.535822836118356 25.910049301729227 27.588671547525834 19.965456314626582 14-15 23.18232906030449 26.141807950034607 27.771420118094113 22.904442871566786 16-17 20.503502592742485 29.91019964387751 28.052252946790297 21.534044816589702 18-19 20.444629929082193 27.531840248816685 32.21961367746079 19.803916144640333 20-21 21.940440588822856 26.16535100194779 32.11867622500543 19.77553218422393 22-23 21.36138645120281 26.486069759148666 31.965135459707874 20.187408329940652 24-25 21.029202305972188 26.629841430585554 32.35472137672782 19.986234886714442 26-27 20.93685307210413 26.741956482847062 32.32972970535042 19.99146073969839 28-29 20.398802060311933 26.991589765794373 32.541652759861805 20.06795541403189 30-31 20.5602960839951 26.893484390132578 32.405610297391945 20.140609228480375 32-33 20.370452778290602 26.990049377266732 32.07177052770713 20.56772731673553 34-35 20.93398263647303 26.730624789901565 32.093148701283475 20.242243872341938 36-37 21.212596829474588 26.446973544276737 31.964408186730438 20.37602143951824 38-39 20.91723274464431 26.70087990647686 32.127410842718525 20.25447650616031 40-41 20.788136459579892 26.954585636023598 32.022929683506625 20.234348220889885 42-43 20.72969508228962 27.193871153119233 31.40207055475314 20.674363209838006 44-45 20.840569652680728 27.345710045294148 31.017470599696434 20.79624970232869 46-47 21.267734713403183 27.671174223996353 30.1535981179991 20.907492944601362 48-49 21.397898023405943 27.86390853148485 29.673686685577277 21.064506759531927 50-51 21.49670066527914 28.25848767612939 29.390899452827 20.85391220576447 52-53 21.577763331892086 28.419620773450895 29.098731080617064 20.90388481403996 54-55 20.892549270526203 28.354403813553464 29.30734116257575 21.445705753344587 56-57 21.40754977265771 28.75842423316702 28.709083047739774 21.1249429464355 58-59 21.558008817068384 28.034272429492617 28.931373958077135 21.476344795361868 60-61 21.2958179962728 28.19188759951675 29.553505870127744 20.9587885340827 62-63 21.49219050207738 28.09311502539823 29.545297373100542 20.869397099423843 64-65 21.278288495295296 28.291141257710123 29.446825476528808 20.98374477046577 66-67 21.13672950626943 28.3742485316412 29.111570011864735 21.377451950224636 68-69 21.225940534400216 28.412855528648258 29.077924194379616 21.28327974257191 70-71 21.132820698161236 28.589473519629134 28.89357879044639 21.38412699176324 72-73 21.123619965229647 28.408766314011995 28.70397152944445 21.763642191313906 74-75 21.07569196427229 28.94261929832685 28.47302110575972 21.50866763164114 76-77 21.12488281092614 28.418839011829256 28.856926197493433 21.599351979751173 78-79 21.033386633439793 28.37277521166196 28.779411525932534 21.814426628965712 80-81 21.08698005303955 28.92170953328803 28.519492937080177 21.47181747659224 82-83 21.10261558042709 28.788553494331143 28.39472355682135 21.714107368420418 84-85 20.907821497813465 28.576574474533707 28.331370902950503 22.184233124702327 86-87 20.954999996993223 28.36883633579909 28.371903246776288 22.304260420431397 88-89 21.071422343107958 28.084756189597638 28.432339433679854 22.411482033614547 90-91 20.762222708028933 28.06825778053358 28.67712661302278 22.49239289841471 92-93 20.775368476378965 28.18837533624278 28.6602127297471 22.376043457631155 94-95 20.478847033906202 28.028349081820974 28.685900808882735 22.806903075390082 96-97 20.464053698604435 27.897644552234002 28.500292559253023 23.13800918990854 98-99 20.371595911621114 28.134079535945606 28.151879673475722 23.34244487895756 100-101 20.232667025316058 28.043816169835022 28.627709003232354 23.09580780161657 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1091.0 1 773.5 2 528.0 3 648.5 4 620.5 5 656.5 6 1132.5 7 2413.0 8 3831.0 9 4459.0 10 4222.0 11 3836.0 12 3976.5 13 4238.5 14 4473.0 15 4621.5 16 4716.5 17 4900.0 18 5036.0 19 5221.5 20 5664.0 21 6227.5 22 6920.5 23 7878.0 24 9059.5 25 10668.0 26 12738.0 27 14931.0 28 16661.5 29 18984.0 30 22372.0 31 25508.5 32 29449.0 33 33665.5 34 37763.0 35 40858.0 36 44886.5 37 49796.0 38 51790.0 39 52369.0 40 52859.0 41 54022.0 42 55236.0 43 57314.0 44 60550.5 45 62138.0 46 60973.5 47 62438.5 48 74657.0 49 73031.0 50 58891.5 51 52002.0 52 46065.5 53 43597.5 54 42107.0 55 38594.0 56 34150.0 57 30355.0 58 28057.5 59 25690.5 60 21884.5 61 17977.5 62 14501.0 63 10544.0 64 6978.5 65 5162.5 66 3730.5 67 2745.5 68 2030.0 69 1294.0 70 930.5 71 737.0 72 598.5 73 573.5 74 475.0 75 342.5 76 236.0 77 137.0 78 81.5 79 57.5 80 38.0 81 22.5 82 22.5 83 15.5 84 9.5 85 8.5 86 5.5 87 1.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07432748956498574 2 0.0 3 0.0 4 0.0 5 0.0019243362994171066 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.2027101871356916E-4 12-13 0.005352060332753828 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010824391684221223 24-25 6.915583576030226E-4 26-27 2.10474282748746E-4 28-29 0.003367588523979936 30-31 2.706097921055306E-4 32-33 0.0 34-35 0.0 36-37 3.006775467839229E-5 38-39 4.20948565497492E-4 40-41 6.013550935678458E-5 42-43 0.01301933777574386 44-45 1.8040652807035373E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.013550935678458E-5 82-83 6.314228482462381E-4 84-85 4.20948565497492E-4 86-87 0.0 88-89 0.0 90-91 0.009351071704980002 92-93 0.003006775467839229 94-95 0.0 96-97 0.0 98-99 1.5033877339196144E-4 100-101 0.011515950041824246 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1662911.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.6356902294479 #Duplication Level Percentage of deduplicated Percentage of total 1 70.31316667275082 29.978503933109447 2 14.783089787172388 12.605744735999938 3 5.594082890237562 7.1552275567807 4 2.8240931250605046 4.816286386367726 5 1.5831868108408107 3.3750131221178177 6 1.027191379740186 2.627700808377705 7 0.6788469376688713 2.0260175423361524 8 0.486843205205678 1.660551686996866 9 0.3628967713824977 1.3925118896937854 >10 2.066723091648165 16.31163724842827 >50 0.17635500174104066 5.203030663524272 >100 0.09533292032023707 7.384479073770761 >500 0.005508014534800037 1.6652422900175723 >1k 0.0022596982706871945 1.6973368168871934 >5k 1.4123114191794966E-4 0.5129704960643535 >10k+ 2.824622838358993E-4 1.587745749527512 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14557 0.8753926097067131 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11811 0.7102605010129827 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8519 0.5122944042104478 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3478 0.20915130154289677 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3363 0.2022357179668665 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2952 0.1775200236212281 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2670 0.16056180998261482 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2056 0.12363860723754909 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1733 0.10421483771530766 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1693 0.10180941734103628 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.2027101871356916E-4 0.0 16-17 0.0 0.0 0.0 1.2027101871356916E-4 0.0 18-19 0.0 0.0 0.0 1.5033877339196144E-4 0.0 20-21 0.0 0.0 0.0 2.706097921055306E-4 0.0 22-23 0.0 0.0 0.0 4.5101632017588433E-4 0.0 24-25 0.0 0.0 0.0 8.118293763165918E-4 0.0 26-27 0.0 0.0 0.0 0.0014733199792412222 0.0 28-29 0.0 0.0 0.0 0.0023753526195929905 0.0 30-31 0.0 0.0 0.0 0.004329756673688489 0.0 32-33 0.0 0.0 0.0 0.006584838274567911 0.0 34-35 0.0 0.0 0.0 0.009621681497085532 0.0 36-37 0.0 0.0 0.0 0.012568321455567976 0.0 38-39 0.0 0.0 0.0 0.016236587526331836 0.0 40-41 0.0 0.0 0.0 0.024535287817568106 0.0 42-43 0.0 0.0 0.0 0.03322486891962348 0.0 44-45 6.013550935678458E-5 0.0 0.0 0.05282904496993525 0.0 46-47 6.013550935678458E-5 0.0 0.0 0.09537491783986034 0.0 48-49 6.013550935678458E-5 0.0 0.0 0.13223798507556928 0.0 50-51 6.013550935678458E-5 0.0 0.0 0.17709907505573058 0.0 52-53 6.013550935678458E-5 0.0 0.0 0.23260415019204275 0.0 54-55 6.013550935678458E-5 0.0 0.0 0.29992585291696305 0.0 56-57 6.013550935678458E-5 0.0 0.0 0.3732310388228835 0.0 58-59 6.013550935678458E-5 0.0 0.0 0.4845719343969701 0.0 60-61 6.013550935678458E-5 0.0 0.0 0.6209893373728359 0.0 62-63 6.013550935678458E-5 0.0 0.0 0.769614248748129 0.0 64-65 6.013550935678458E-5 0.0 0.0 0.8998076265055677 0.0 66-67 6.013550935678458E-5 0.0 0.0 1.0871898736613084 0.0 68-69 6.013550935678458E-5 0.0 0.0 1.316877451649547 0.0 70-71 6.013550935678458E-5 0.0 0.0 1.515685445583077 0.0 72-73 6.013550935678458E-5 0.0 0.0 1.8185880062132007 0.0 74-75 6.013550935678458E-5 0.0 0.0 2.078463609898545 0.0 76-77 6.013550935678458E-5 0.0 0.0 2.324628317450543 0.0 78-79 6.013550935678458E-5 0.0 0.0 2.6345366649207325 0.0 80-81 6.013550935678458E-5 0.0 0.0 2.955028862037716 0.0 82-83 6.013550935678458E-5 0.0 0.0 3.3176459834591268 0.0 84-85 6.013550935678458E-5 0.0 0.0 3.803961847627444 0.0 86-87 6.013550935678458E-5 0.0 0.0 4.388839811631531 0.0 88-89 6.013550935678458E-5 0.0 0.0 5.061305145013774 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 21170 0.0 25.844799 1 GTACATG 14330 0.0 25.044909 1 TACATGG 14345 0.0 24.436268 2 GGTATCA 15490 0.0 24.02863 1 CGACCGA 60 1.7121549E-4 23.749203 46-47 GAGTACT 11665 0.0 23.332539 12-13 ACATGGG 14405 0.0 23.180408 3 GGACCGT 145 2.3357605E-5 22.930265 6 AGTACTT 11875 0.0 22.379921 12-13 TAAGGTG 590 0.0 21.73656 5 GTACTTT 12455 0.0 21.718462 14-15 ATCAACG 25340 0.0 21.443638 3 GTATAGG 510 0.0 21.437609 1 TCAACGC 25445 0.0 21.35515 4 CAACGCA 25675 0.0 21.163847 5 TATCAAC 25765 0.0 21.16366 2 CATGGGG 3885 0.0 21.028894 4 AACGCAG 26015 0.0 20.905508 6 CATGGGA 10485 0.0 20.61209 4 TACTTTT 13590 0.0 19.467707 14-15 >>END_MODULE