FastQCFastQC Report
Thu 26 May 2016
SRR937411_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937411_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324978
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92390.3973801042418466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69810.3002609056945915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46190.19866854654108554No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35600.15311972844474225No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30610.13165715976667305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA125150.032.245411
GGTATCA84000.031.6317751
TCAACGC150900.026.7611874
ATCAACG150850.026.737413
TATCAAC151600.026.606282
CAACGCA152300.026.4857045
AACGCAG155650.025.8795596
TATAGCG2059.549694E-1025.4942725
GTACATG99450.023.5153451
ACGCAGA171450.023.35667
GAGTACT85750.023.20879612-13
CGCAGAG173050.023.0033958
TACATGG98100.022.8101222
ACATGGG97750.021.9673983
GTACTTT91200.021.68956214-15
AGTACTT90200.021.56354112-13
GCAGAGT181950.021.3560419
AGAGTAC140150.021.13217210-11
GTGTAGC13700.020.8171711
GAGTACA81800.018.478421
Produced by FastQC (version 0.11.5)