##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937411_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2324978 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.658145582452825 33.0 31.0 34.0 30.0 34.0 2 31.769719111320622 33.0 31.0 34.0 30.0 34.0 3 31.803217922922283 33.0 31.0 34.0 30.0 34.0 4 35.43367937244997 37.0 35.0 37.0 33.0 37.0 5 35.444503130782316 37.0 35.0 37.0 33.0 37.0 6 35.47972927055654 37.0 35.0 37.0 33.0 37.0 7 35.411250343014 37.0 35.0 37.0 33.0 37.0 8 35.39359426196721 37.0 35.0 37.0 33.0 37.0 9 36.98474007065873 39.0 37.0 39.0 33.0 39.0 10-11 36.864931410103665 39.0 37.0 39.0 33.0 39.0 12-13 36.71713882884053 39.0 37.0 39.0 32.5 39.0 14-15 37.79770690303306 40.0 37.5 41.0 32.5 41.0 16-17 37.71702076320722 40.0 37.0 41.0 32.5 41.0 18-19 37.63846453600851 40.0 37.0 41.0 32.0 41.0 20-21 37.50136926026827 40.0 37.0 41.0 32.0 41.0 22-23 37.578152352409354 40.0 37.0 41.0 32.0 41.0 24-25 37.54782776439175 40.0 37.0 41.0 32.0 41.0 26-27 37.464402673917775 40.0 37.0 41.0 32.0 41.0 28-29 37.32232606071972 40.0 37.0 41.0 32.0 41.0 30-31 37.07029700926202 39.0 36.5 41.0 31.0 41.0 32-33 36.821403256288875 39.0 36.0 41.0 30.5 41.0 34-35 36.81004744991135 39.0 36.0 41.0 30.0 41.0 36-37 36.66995386623013 39.0 36.0 41.0 30.0 41.0 38-39 36.51377367871868 39.0 35.5 41.0 30.0 41.0 40-41 36.27644089535471 39.0 35.0 40.5 30.0 41.0 42-43 35.999339348587384 39.0 35.0 40.0 29.0 41.0 44-45 35.74075432111616 39.0 35.0 40.0 28.5 41.0 46-47 35.33270959983277 38.5 35.0 40.0 26.5 41.0 48-49 34.97752537873477 38.0 34.5 40.0 25.5 41.0 50-51 34.01024418295571 37.5 33.5 39.5 23.5 40.5 52-53 34.12359837383407 38.0 33.5 39.5 24.0 40.5 54-55 34.42132054582882 38.0 34.0 40.0 23.5 41.0 56-57 34.13521375256024 38.0 33.5 40.0 23.0 41.0 58-59 33.852976888383466 38.0 33.0 40.0 22.0 41.0 60-61 33.503053577281165 37.5 33.0 40.0 20.5 41.0 62-63 33.096979842389906 37.0 32.0 40.0 18.5 41.0 64-65 32.65218638627978 36.0 31.5 39.0 17.0 41.0 66-67 32.205022585159945 36.0 31.0 39.0 13.0 40.0 68-69 31.691989773666677 35.0 31.0 39.0 8.0 40.0 70-71 31.19143772543224 35.0 30.0 38.0 7.5 40.0 72-73 30.6748091809901 35.0 29.5 37.5 4.0 39.5 74-75 30.093085181881293 34.0 29.0 37.0 2.0 39.0 76-77 29.53259364174629 34.0 29.0 36.0 2.0 39.0 78-79 28.934749275046904 34.0 28.0 36.0 2.0 38.5 80-81 28.300084990051516 34.0 26.5 35.0 2.0 37.0 82-83 27.698954785808727 33.0 26.0 35.0 2.0 37.0 84-85 27.05570977445808 33.0 25.5 35.0 2.0 36.0 86-87 26.40533523327963 32.0 24.5 35.0 2.0 36.0 88-89 25.78293149440554 32.0 22.0 34.5 2.0 35.0 90-91 25.149026571434224 31.5 19.5 34.0 2.0 35.0 92-93 24.39951087709217 31.0 14.5 34.0 2.0 35.0 94-95 23.553323085207687 31.0 4.5 34.0 2.0 35.0 96-97 22.622264597772535 30.5 2.0 34.0 2.0 35.0 98-99 21.36064685343259 29.5 2.0 34.0 2.0 35.0 100-101 18.29287890035949 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1467583058377926 1101 2 0.20798455646686165 1101 3 0.0862142298199089 1101 4 0.014192084313798148 1101 5 0.04664468101689323 1101 6 0.04297396628315653 1101 7 0.16377406663794858 1101 8 0.07758175978108284 1101 9 0.23950761073368199 1101 10-11 0.23690579786554622 1101 12-13 0.20891200114051145 1101 14-15 0.35322634536801445 1101 16-17 0.4011972868223097 1101 18-19 0.4028539290557873 1101 20-21 0.4761837887404923 1101 22-23 0.4485369374997674 1101 24-25 0.3697574403868984 1101 26-27 0.3298907171307164 1101 28-29 0.2361280087256219 1101 30-31 0.36102914495046434 1101 32-33 0.32824210615401483 1101 34-35 0.4077763124565905 1101 36-37 0.4538687220502169 1101 38-39 0.44062035322811255 1101 40-41 0.5449872925266632 1101 42-43 0.5852428503293865 1101 44-45 0.6171034663537895 1101 46-47 0.7797556262586909 1101 48-49 0.7021390285932867 1101 50-51 0.6710635497360897 1101 52-53 0.7022457670614131 1101 54-55 0.7609924453810137 1101 56-57 0.6650589464031924 1101 58-59 0.7636257498128103 1101 60-61 0.8864596341062949 1101 62-63 1.0066261199521378 1101 64-65 0.8030466774725582 1101 66-67 0.9597374399646554 1101 68-69 1.0956153529186246 1101 70-71 1.016375206536921 1101 72-73 0.9249787610206432 1101 74-75 1.2061554401050358 1101 76-77 1.23509954395443 1101 78-79 1.4174006674405177 1101 80-81 1.588533639987503 1101 82-83 1.4549218824424592 1101 84-85 1.6265452825176858 1101 86-87 1.7435854779493631 1101 88-89 1.7030995968264335 1101 90-91 1.699111854422572 1101 92-93 2.033834497315482 1101 94-95 2.123048889591235 1101 96-97 2.2515923010425567 1101 98-99 2.3490269558449413 1101 100-101 2.4484431754012697 1102 1 0.361761386964222 1102 2 0.24829742072653715 1102 3 0.1909377822473175 1102 4 0.21535726981245773 1102 5 0.275543716587201 1102 6 0.25106077238007174 1102 7 0.19077750413563166 1102 8 0.2628300688737468 1102 9 0.40496573339963504 1102 10-11 0.36319894291607113 1102 12-13 0.34609570309535087 1102 14-15 0.5928181517990296 1102 16-17 0.5851374906963684 1102 18-19 0.5306298012372963 1102 20-21 0.46717962365151067 1102 22-23 0.4986186059111688 1102 24-25 0.47331068447892477 1102 26-27 0.6293701181300335 1102 28-29 0.5621180154818219 1102 30-31 0.612884502769468 1102 32-33 0.6411399241225553 1102 34-35 0.7309163143869313 1102 36-37 0.84539547587935 1102 38-39 0.695748832488043 1102 40-41 0.7957995962440592 1102 42-43 0.8802267765011464 1102 44-45 0.9097881297685717 1102 46-47 1.0263278545441707 1102 48-49 1.118939334478604 1102 50-51 0.9748620217943582 1102 52-53 0.9910903817721177 1102 54-55 1.201910101349121 1102 56-57 1.2976164298282171 1102 58-59 1.3545178658413448 1102 60-61 1.3233151032913142 1102 62-63 1.3106089398881409 1102 64-65 1.2318944846757134 1102 66-67 1.3583698652937457 1102 68-69 1.3763742009485576 1102 70-71 1.4458044185852401 1102 72-73 1.528393614277359 1102 74-75 1.4948254329033652 1102 76-77 1.6051563406223615 1102 78-79 1.714987737103673 1102 80-81 1.8745241664517849 1102 82-83 1.8066256339923044 1102 84-85 1.7658818380410715 1102 86-87 1.7549245429202962 1102 88-89 1.7978369586397704 1102 90-91 2.0689041196815694 1102 92-93 2.0764912449823605 1102 94-95 2.0457870829374905 1102 96-97 2.0585442874410553 1102 98-99 2.3870256343015583 1102 100-101 2.3608012394144566 1103 1 0.27214042875434785 1103 2 0.3285765612844962 1103 3 0.27631172373142476 1103 4 0.17702498892322183 1103 5 0.2693271721659656 1103 6 0.2991199035469947 1103 7 0.26162285961886056 1103 8 0.19450410264910545 1103 9 0.3401332339258545 1103 10-11 0.29423470982398925 1103 12-13 0.22121333396273002 1103 14-15 0.3719536904075369 1103 16-17 0.3883362730994975 1103 18-19 0.409947493632167 1103 20-21 0.30378573430386524 1103 22-23 0.19200844739689416 1103 24-25 0.1921220703049329 1103 26-27 0.23908288184824045 1103 28-29 0.1309548218806853 1103 30-31 0.27584899492471493 1103 32-33 0.2603628965485214 1103 34-35 0.45340218734883564 1103 36-37 0.5659961785881791 1103 38-39 0.5566447052880292 1103 40-41 0.6290501912797595 1103 42-43 0.5058800204576883 1103 44-45 0.7508238966764864 1103 46-47 0.5678304208886331 1103 48-49 0.6925820863770582 1103 50-51 0.8183705465915594 1103 52-53 0.8212636302622656 1103 54-55 0.9807520890112329 1103 56-57 0.8859359194737877 1103 58-59 0.7795498439505124 1103 60-61 0.7130149138513744 1103 62-63 0.8324997771479019 1103 64-65 0.8124025723038315 1103 66-67 1.304894371049805 1103 68-69 1.0894052399537593 1103 70-71 1.1841991984625082 1103 72-73 1.2537939478211761 1103 74-75 1.2603530312259323 1103 76-77 1.007534540457204 1103 78-79 1.0135046004964288 1103 80-81 0.9069944956239127 1103 82-83 1.2112510448502896 1103 84-85 1.3343315069259667 1103 86-87 1.4371808211541328 1103 88-89 1.628406897598012 1103 90-91 1.5734459826695364 1103 92-93 1.8612165570725168 1103 94-95 1.7236520786467544 1103 96-97 2.080397560542206 1103 98-99 2.1344320882125984 1103 100-101 2.158504158732473 1104 1 0.15015559635297393 1104 2 0.17423730659364978 1104 3 0.08452954726418582 1104 4 0.1520408853681161 1104 5 0.11624064246302623 1104 6 0.15492916192463468 1104 7 0.14561384628549234 1104 8 0.09495241186327519 1104 9 0.2205241915172209 1104 10-11 0.15770454848899362 1104 12-13 0.20020137708581132 1104 14-15 0.4214507340944351 1104 16-17 0.36878430072886204 1104 18-19 0.34356289199937606 1104 20-21 0.25812331913128617 1104 22-23 0.20732240463922125 1104 24-25 0.18870556249357406 1104 26-27 0.27820499863259585 1104 28-29 0.3581821413700865 1104 30-31 0.38219457414139413 1104 32-33 0.35868560060092136 1104 34-35 0.3828607717892467 1104 36-37 0.315583624096611 1104 38-39 0.36709261504469026 1104 40-41 0.43201875004356083 1104 42-43 0.36888688130436265 1104 44-45 0.3284095164675733 1104 46-47 0.5008227310038791 1104 48-49 0.2746941820163542 1104 50-51 0.1822165956619628 1104 52-53 0.27846470342782226 1104 54-55 0.3552790428987791 1104 56-57 0.5077468896251389 1104 58-59 0.5755477052156337 1104 60-61 0.5216754149020844 1104 62-63 0.48273345979919924 1104 64-65 0.5540090529455242 1104 66-67 0.6202698512992484 1104 68-69 0.5893900147284761 1104 70-71 0.6149006851648338 1104 72-73 0.7234761040535993 1104 74-75 0.6244999831358271 1104 76-77 0.6900334223035642 1104 78-79 0.7155584015312222 1104 80-81 0.7906683719531742 1104 82-83 1.0112068098409175 1104 84-85 0.9416413504106487 1104 86-87 0.9999616283333665 1104 88-89 1.1630478867858116 1104 90-91 1.1622514475103998 1104 92-93 1.2637091392772746 1104 94-95 1.2965741955620764 1104 96-97 1.4850720976135001 1104 98-99 1.73894163622969 1104 100-101 1.7100250113974766 1105 1 0.12050473389171046 1105 2 -0.021156739315859596 1105 3 0.16200941654145495 1105 4 0.20287967713026234 1105 5 0.19678884918783268 1105 6 0.21263723375193422 1105 7 0.19284381597176292 1105 8 0.18845205915046392 1105 9 0.2549249509291869 1105 10-11 0.19552884314878582 1105 12-13 0.29005989746875827 1105 14-15 0.3867869543611846 1105 16-17 0.3480694507894526 1105 18-19 0.42101573704522366 1105 20-21 0.4237953698324688 1105 22-23 0.5581206911165779 1105 24-25 0.3205817151003387 1105 26-27 0.5092581391433768 1105 28-29 0.43186479550993084 1105 30-31 0.3939459990483556 1105 32-33 0.4465656055584688 1105 34-35 0.5732920660977214 1105 36-37 0.5546318990032475 1105 38-39 0.5569361323198478 1105 40-41 0.4425298559154953 1105 42-43 0.5532416839917573 1105 44-45 0.4019221819793444 1105 46-47 0.4057969912000132 1105 48-49 0.5257444772172519 1105 50-51 0.5030383310959863 1105 52-53 0.5038096739543221 1105 54-55 0.7255952698936241 1105 56-57 0.8469862600164575 1105 58-59 0.7114255407797074 1105 60-61 0.7257367064801841 1105 62-63 0.5597794659128894 1105 64-65 0.6549875009656674 1105 66-67 0.8437227373473846 1105 68-69 0.6829088430074997 1105 70-71 0.8004448646579121 1105 72-73 1.004982863372998 1105 74-75 0.878923725569436 1105 76-77 0.859931115397135 1105 78-79 0.9080754539765543 1105 80-81 0.8981791513599617 1105 82-83 1.0512137771226442 1105 84-85 1.0696379726923446 1105 86-87 1.1074516736315694 1105 88-89 1.1755132794618746 1105 90-91 1.1963415059169087 1105 92-93 1.2962608355447927 1105 94-95 1.3212339620416564 1105 96-97 1.1619435178735351 1105 98-99 1.2485177658192654 1105 100-101 1.410970129532199 1106 1 0.12235425832695412 1106 2 -0.5189452092879172 1106 3 -0.001973941552080305 1106 4 0.14676363486195498 1106 5 -0.040629617836970056 1106 6 -0.03986016901456679 1106 7 0.12398866342549297 1106 8 0.13821472621373232 1106 9 0.08882730584502241 1106 10-11 -0.20722702997656484 1106 12-13 -0.1500642685428133 1106 14-15 0.013166675074813838 1106 16-17 0.16916459806225959 1106 18-19 0.34873664898334766 1106 20-21 0.3160716032528015 1106 22-23 0.23340957375867077 1106 24-25 0.2601139788147435 1106 26-27 0.3083736360879854 1106 28-29 0.38538071694627263 1106 30-31 0.2544932185784603 1106 32-33 0.3608983031348032 1106 34-35 0.4185966438205142 1106 36-37 0.2755498296931975 1106 38-39 0.341755268442121 1106 40-41 0.5390039377859566 1106 42-43 0.5113697037694109 1106 44-45 0.5911563154294868 1106 46-47 0.571160274299281 1106 48-49 0.6589799682564603 1106 50-51 0.624899383053183 1106 52-53 0.6140026509913525 1106 54-55 0.7998058716182825 1106 56-57 0.5841745579473354 1106 58-59 0.39147960463979103 1106 60-61 0.4732510164604733 1106 62-63 0.5098030820790953 1106 64-65 0.5840049867346764 1106 66-67 0.6811002369973593 1106 68-69 0.8595150010794619 1106 70-71 0.8908601877635647 1106 72-73 0.9317602182207416 1106 74-75 0.6976602525057558 1106 76-77 0.9539555988513797 1106 78-79 1.038661685125163 1106 80-81 0.9393159524290411 1106 82-83 0.7719513859899045 1106 84-85 0.5611470484367729 1106 86-87 0.6894488004675416 1106 88-89 1.0198111057394996 1106 90-91 0.7573409464289576 1106 92-93 1.164854167341847 1106 94-95 1.1650357671121334 1106 96-97 1.1973255468154598 1106 98-99 1.3700979842429248 1106 100-101 1.150029743055132 1107 1 0.1761869122763855 1107 2 0.2548994084604068 1107 3 0.0929596522197933 1107 4 0.13836696348584354 1107 5 0.15408256069627413 1107 6 0.22484930820256466 1107 7 0.22812044131050158 1107 8 0.1373506526174424 1107 9 0.26225624143958726 1107 10-11 0.1811502050535836 1107 12-13 0.2556961963538811 1107 14-15 0.2813020351482862 1107 16-17 0.3298232017154632 1107 18-19 0.3688723635683502 1107 20-21 0.36059070236871094 1107 22-23 0.3393218259747002 1107 24-25 0.49950001735494 1107 26-27 0.43955149680395067 1107 28-29 0.46923090145254065 1107 30-31 0.4052002045603871 1107 32-33 0.44288739862255966 1107 34-35 0.5165321933915976 1107 36-37 0.5890567771345516 1107 38-39 0.7185774741487947 1107 40-41 0.5721079971554417 1107 42-43 0.4736806478455975 1107 44-45 0.47166275717054873 1107 46-47 0.7258390059435058 1107 48-49 0.6690634657637773 1107 50-51 0.5141951427978526 1107 52-53 0.4079471653018558 1107 54-55 0.6630003333602872 1107 56-57 0.7847557753623491 1107 58-59 0.7065088663231691 1107 60-61 0.6011895183634692 1107 62-63 0.6026094135469933 1107 64-65 0.6381399216259993 1107 66-67 0.8139055489549634 1107 68-69 0.9696189274105755 1107 70-71 0.94980806085848 1107 72-73 1.0128972116346624 1107 74-75 0.836927579884609 1107 76-77 0.9777011136336036 1107 78-79 1.0079228703406393 1107 80-81 1.1160505729327426 1107 82-83 1.2470538052608084 1107 84-85 1.2272975880145296 1107 86-87 1.0470579677187857 1107 88-89 1.1529751771924985 1107 90-91 1.185341796793228 1107 92-93 1.2956092595351194 1107 94-95 1.3049615282258067 1107 96-97 1.4659082942682744 1107 98-99 1.5167601403488717 1107 100-101 1.2091845110904735 1108 1 0.2618473391533165 1108 2 0.3594282292143127 1108 3 0.2368255072003116 1108 4 0.2774738424689218 1108 5 0.31999174237285644 1108 6 0.4189307696940716 1108 7 0.33697143376227245 1108 8 0.2738104610306067 1108 9 0.3379767460238625 1108 10-11 0.3507385615032348 1108 12-13 0.4835520554993238 1108 14-15 0.5071184472596713 1108 16-17 0.5613314203230075 1108 18-19 0.4228511281242149 1108 20-21 0.5063577058432926 1108 22-23 0.5120620654867807 1108 24-25 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1.6755368822990278 2316 100-101 2.181013112426033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9104.0 3 1734.0 4 1702.0 5 2029.0 6 2320.0 7 2832.0 8 3439.0 9 4505.0 10 5696.0 11 7396.0 12 9813.0 13 12353.0 14 14121.0 15 17405.0 16 21604.0 17 27032.0 18 23812.0 19 18463.0 20 19124.0 21 20298.0 22 22626.0 23 25110.0 24 28823.0 25 33055.0 26 38341.0 27 44456.0 28 51977.0 29 62065.0 30 73395.0 31 89364.0 32 110550.0 33 139843.0 34 179993.0 35 237874.0 36 315202.0 37 377938.0 38 246908.0 39 22673.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25999568686651 19.13534612896269 14.939831787793834 22.66482639637697 2 14.881572168004329 20.26132377891172 41.105872563159 23.751231489924958 3 18.85621354635541 24.730230095577102 30.282049026810565 26.13150733125692 4 12.061515644064654 16.78763133727974 38.50168792925787 32.649165089397734 5 13.126817839641214 37.535257358235455 34.888767400169186 14.44915740195415 6 30.953252240148583 36.634387148191394 18.185666066247798 14.226694545412228 7 27.90720629233785 32.89649952500574 21.291596480732128 17.904697701924288 8 25.82113445475948 34.71324840885129 20.467090398280707 18.998526738108517 9 26.0208260410003 16.77114460459318 20.977089285745077 36.23094006866145 10-11 24.277708333737497 26.85912976450603 28.135075089018063 20.728086812738418 12-13 25.46343665070741 24.26135186742258 28.52508760060032 21.75012388126969 14-15 22.2915477267305 25.529106259187873 27.14283158837806 25.036514425703572 16-17 21.38000502566061 28.928107623272 27.723694696721207 21.968192654346183 18-19 21.278276094276094 27.32416430976431 30.28717037037037 21.110389225589227 20-21 22.156394478572295 26.382522690961157 30.276092935211967 21.18498989525458 22-23 21.75554539385474 26.455818078929983 30.178462835398978 21.6101736918163 24-25 21.624535518666423 26.492659778023015 30.307295225768687 21.57550947754187 26-27 21.400798081982906 26.740831948103555 30.26173623989522 21.596633730018322 28-29 21.192905899703042 26.933904817609864 30.383887695063944 21.489301587623146 30-31 21.45802619213891 26.57536490529274 30.278259145263775 21.68834975730457 32-33 21.25453292304085 26.753459418242727 30.18697216473827 21.80503549397816 34-35 21.717186955817088 26.754639671171283 30.035254431911834 21.4929189410998 36-37 21.70977716211196 26.486771182357096 30.100462812510504 21.702988843020442 38-39 21.358312212574884 26.896440119686932 30.1123691866917 21.63287848104648 40-41 21.64359735213384 26.74986085580093 30.015542347780126 21.59099944428511 42-43 21.442481810371003 26.775383860810848 29.756343123325717 22.025791205492435 44-45 21.568678577100584 26.886848866553564 29.53755144021837 22.006921116127483 46-47 21.775542222681977 27.17701544774318 29.115289127961102 21.932153201613737 48-49 21.79083112464176 27.13041934033649 29.085030566797172 21.99371896822458 50-51 21.638073142175298 27.34712185247069 29.021953170936943 21.992851834417067 52-53 21.98446704155753 27.50597699221757 28.58124279619119 21.928313170033707 54-55 21.710511427985836 27.220289618314258 28.845322026935953 22.22387692676395 56-57 21.862722261212426 27.501079024221642 28.57090218060937 22.065296533956563 58-59 22.03107648592151 27.161135690514122 28.700868785058326 22.10691903850604 60-61 21.940173310329865 27.163788108654117 29.06286717263096 21.833171408385063 62-63 21.733876563172018 27.275859512972094 29.070108194904247 21.92015572895164 64-65 21.597689360803596 27.275303050543613 28.97820363100445 22.148803957648337 66-67 21.648605978934814 27.414113602734968 28.6438032746475 22.293477143682715 68-69 21.63242492468713 27.255362183748964 28.819753961076692 22.292458930487204 70-71 21.70581622580451 27.186862245310227 28.797298220368805 22.310023308516456 72-73 21.746041699123236 27.05189625016457 28.86961498374101 22.33244706697118 74-75 21.564063456874933 27.46557782155415 28.609011717482357 22.361347004088554 76-77 21.596570619030246 27.203406796568636 28.693783320342277 22.506239264058845 78-79 21.68677321149229 27.219012822919243 28.657348573807695 22.43686539178077 80-81 21.660149915797618 27.373995416545903 28.55931500310843 22.40653966454805 82-83 21.67758648404865 27.28300175961117 28.658239380382426 22.381172375957757 84-85 21.69432095754351 27.146422343945964 28.536983120867397 22.622273577643128 86-87 21.543936897530884 27.13792708931911 28.734600200421745 22.583535812728268 88-89 21.762650347210773 26.995645832296344 28.47160676265358 22.7700970578393 90-91 21.646690490837532 26.891721405877465 28.73539763992032 22.72619046336468 92-93 21.79271114742745 26.69515803963322 28.850025524150112 22.66210528878922 94-95 21.587587108323635 26.66819940972872 28.785065537694383 22.959147944253257 96-97 21.50782364987009 26.723195166841247 28.887049092048052 22.88193209124061 98-99 21.48393458675392 26.784021128630194 28.541665948357604 23.190378336258288 100-101 21.819136152407644 25.903296834946232 29.2735853508286 23.003981661817523 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3266.0 1 1839.0 2 389.5 3 381.5 4 333.5 5 296.0 6 482.0 7 951.5 8 1603.5 9 2063.0 10 2065.0 11 1969.5 12 2120.0 13 2367.5 14 2573.5 15 2790.0 16 3021.0 17 3227.5 18 3430.0 19 3762.5 20 4198.0 21 4795.5 22 5731.0 23 6933.0 24 8549.5 25 10771.0 26 13818.5 27 17621.5 28 21150.5 29 25031.0 30 29823.5 31 34925.0 32 40484.5 33 45979.5 34 51711.0 35 57081.5 36 62611.0 37 68380.5 38 71808.0 39 73694.0 40 75700.5 41 78234.5 42 80743.0 43 84329.5 44 87971.0 45 89706.5 46 89107.5 47 90459.5 48 96084.0 49 95285.5 50 88614.0 51 82329.0 52 75967.0 53 71367.0 54 66985.5 55 60688.5 56 53960.0 57 47853.5 58 42503.0 59 37184.0 60 31228.0 61 25542.5 62 20417.0 63 15511.5 64 11671.5 65 9028.5 66 6681.0 67 4987.0 68 3609.5 69 2403.0 70 1694.0 71 1228.0 72 935.0 73 701.5 74 550.5 75 437.0 76 289.5 77 177.0 78 109.5 79 80.5 80 53.5 81 35.5 82 32.0 83 18.0 84 10.5 85 11.5 86 10.0 87 9.0 88 10.5 89 9.5 90 8.0 91 4.0 92 2.5 93 3.5 94 1.0 95 1.0 96 3.0 97 2.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27862629237782033 2 0.24228186245203182 3 0.24060442722468772 4 0.239486137073125 5 0.24103453882144263 6 0.21208802835983825 7 0.21290524039367253 8 0.21316330735172548 9 0.22322791871579 10-11 0.2307333660791629 12-13 0.22436771444719047 14-15 0.2152493485959867 16-17 0.2101095150147657 18-19 0.2006685654659958 20-21 0.20563635440851483 22-23 0.20296966250863446 24-25 0.2057008711480281 26-27 0.20064705988615808 28-29 0.2123676008977289 30-31 0.2235720079931939 32-33 0.21535687649517546 34-35 0.21445364214199014 36-37 0.20714174499715693 38-39 0.19746423407017186 40-41 0.1954212039855861 42-43 0.2050987149125712 44-45 0.20161481097885656 46-47 0.21066866009054708 48-49 0.21406654170491074 50-51 0.22460427582540565 52-53 0.23477641508865887 54-55 0.21477622583955633 56-57 0.19761477312903605 58-59 0.18843189053831907 60-61 0.19176525541316952 62-63 0.1950986202880199 64-65 0.18959319184955728 66-67 0.19417388035499691 68-69 0.19318462368246064 70-71 0.1970556280532547 72-73 0.19653949413714883 74-75 0.19780832334757575 76-77 0.20613098274478298 78-79 0.20630302738348494 80-81 0.2010986770627507 82-83 0.196948100154066 84-85 0.18630283813438236 86-87 0.1880232845214019 88-89 0.19602336022104294 90-91 0.1937867799179175 92-93 0.19899113023865173 94-95 0.19438893615337435 96-97 0.20101265474339972 98-99 0.20275460671025702 100-101 0.19202332237122244 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2324978.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.79093657117254 #Duplication Level Percentage of deduplicated Percentage of total 1 76.77230645024767 43.59971186038635 2 13.287408160827644 15.092087081136857 3 4.300715733523959 7.3272502329960885 4 1.9628873977222838 4.4589685480160055 5 1.0007351605307362 2.84163435131216 6 0.6001624108639511 2.045027124466598 7 0.40035993679039306 1.59157710431313 8 0.27902456901640876 1.2676853280647695 9 0.19578178030546356 1.0006767600406936 >10 1.0698780759766613 11.140628142676496 >50 0.0872624373507929 3.3949351552690765 >100 0.04112459455927208 4.090407887902785 >500 0.0015182530869330977 0.5611393800515402 >1k 6.832138891195314E-4 0.8890023936722434 >5k 1.518253086932292E-4 0.6992686496951549 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9239 0.3973801042418466 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6981 0.3002609056945915 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4619 0.19866854654108554 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3560 0.15311972844474225 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3061 0.13165715976667305 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.30111596754894E-5 0.0 0.0 0.0 0.0 3 4.30111596754894E-5 0.0 0.0 0.0 0.0 4 4.30111596754894E-5 0.0 0.0 0.0 0.0 5 8.60223193509788E-5 0.0 0.0 0.0 0.0 6 8.60223193509788E-5 0.0 0.0 0.0 0.0 7 8.60223193509788E-5 0.0 0.0 4.30111596754894E-5 0.0 8 8.60223193509788E-5 0.0 0.0 4.30111596754894E-5 0.0 9 8.60223193509788E-5 0.0 0.0 1.720446387019576E-4 4.30111596754894E-5 10-11 8.60223193509788E-5 0.0 0.0 1.935502185397023E-4 4.30111596754894E-5 12-13 8.60223193509788E-5 0.0 0.0 2.580669580529364E-4 4.30111596754894E-5 14-15 1.0752789918872351E-4 0.0 0.0 3.6559485724165994E-4 8.60223193509788E-5 16-17 1.290334790264682E-4 0.0 0.0 4.516171765926387E-4 8.60223193509788E-5 18-19 1.5053905886421292E-4 0.0 0.0 4.946283362681281E-4 8.60223193509788E-5 20-21 1.720446387019576E-4 0.0 0.0 5.806506556191069E-4 8.60223193509788E-5 22-23 1.720446387019576E-4 0.0 0.0 7.742008741588092E-4 8.60223193509788E-5 24-25 2.1505579837744702E-4 0.0 0.0 0.001010762252374001 8.60223193509788E-5 26-27 2.1505579837744702E-4 0.0 0.0 0.0013978626894534055 8.60223193509788E-5 28-29 2.795725378906811E-4 0.0 0.0 0.002602175160367109 8.60223193509788E-5 30-31 3.0107811772842584E-4 0.0 0.0 0.005440911698949409 8.60223193509788E-5 32-33 3.0107811772842584E-4 0.0 0.0 0.009053849111690519 2.580669580529364E-4 34-35 3.225836975661705E-4 0.0 0.0 0.012946359062322312 2.580669580529364E-4 36-37 3.440892774039152E-4 0.0 0.0 0.01748403640808644 2.580669580529364E-4 38-39 3.871004370794046E-4 0.0 0.0 0.02191418585466185 2.795725378906811E-4 40-41 3.871004370794046E-4 0.0 0.0 0.025505617687565214 3.440892774039152E-4 42-43 4.3011159675489404E-4 0.0 0.0 0.030451901050246498 3.440892774039152E-4 44-45 4.731227564303834E-4 0.0 0.0 0.03460247795893122 3.440892774039152E-4 46-47 6.236618152945963E-4 0.0 0.0 0.04286062061662519 3.440892774039152E-4 48-49 6.45167395132341E-4 0.0 0.0 0.05430158909030537 3.440892774039152E-4 50-51 6.45167395132341E-4 0.0 0.0 0.06425867255518117 3.440892774039152E-4 52-53 6.45167395132341E-4 0.0 0.0 0.08514059057763126 3.440892774039152E-4 54-55 6.45167395132341E-4 0.0 0.0 0.12727002147977315 3.440892774039152E-4 56-57 6.45167395132341E-4 0.0 0.0 0.19602336022104294 3.440892774039152E-4 58-59 6.45167395132341E-4 0.0 0.0 0.26802404151781223 3.440892774039152E-4 60-61 6.45167395132341E-4 0.0 0.0 0.33146550203915903 3.440892774039152E-4 62-63 6.45167395132341E-4 0.0 0.0 0.41454155695236683 3.440892774039152E-4 64-65 6.881785548078304E-4 0.0 0.0 0.5125639898528072 3.440892774039152E-4 66-67 6.881785548078304E-4 0.0 0.0 0.6652751122806324 3.440892774039152E-4 68-69 6.881785548078304E-4 0.0 0.0 0.8533414079617098 3.440892774039152E-4 70-71 6.881785548078304E-4 0.0 0.0 1.033321605623795 3.440892774039152E-4 72-73 6.881785548078304E-4 0.0 0.0 1.2253664335748553 3.440892774039152E-4 74-75 6.881785548078304E-4 0.0 0.0 1.4357340155476739 3.871004370794046E-4 76-77 6.881785548078304E-4 0.0 0.0 1.6788976067730532 3.871004370794046E-4 78-79 6.881785548078304E-4 0.0 0.0 1.900383573521986 3.871004370794046E-4 80-81 8.172120338342986E-4 0.0 0.0 2.1388159371830615 3.871004370794046E-4 82-83 8.172120338342986E-4 0.0 0.0 2.4135282140304124 3.871004370794046E-4 84-85 8.387176136720434E-4 0.0 0.0 2.7776391862632677 4.3011159675489404E-4 86-87 8.602231935097881E-4 2.15055798377447E-5 0.0 3.1872989765924666 4.3011159675489404E-4 88-89 8.602231935097881E-4 4.30111596754894E-5 0.0 3.685540250273336 4.3011159675489404E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12515 0.0 32.24541 1 GGTATCA 8400 0.0 31.631775 1 TCAACGC 15090 0.0 26.761187 4 ATCAACG 15085 0.0 26.73741 3 TATCAAC 15160 0.0 26.60628 2 CAACGCA 15230 0.0 26.485704 5 AACGCAG 15565 0.0 25.879559 6 TATAGCG 205 9.549694E-10 25.494272 5 GTACATG 9945 0.0 23.515345 1 ACGCAGA 17145 0.0 23.3566 7 GAGTACT 8575 0.0 23.208796 12-13 CGCAGAG 17305 0.0 23.003395 8 TACATGG 9810 0.0 22.810122 2 ACATGGG 9775 0.0 21.967398 3 GTACTTT 9120 0.0 21.689562 14-15 AGTACTT 9020 0.0 21.563541 12-13 GCAGAGT 18195 0.0 21.356041 9 AGAGTAC 14015 0.0 21.132172 10-11 GTGTAGC 1370 0.0 20.817171 1 GAGTACA 8180 0.0 18.47842 1 >>END_MODULE