FastQCFastQC Report
Thu 26 May 2016
SRR937411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937411_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324978
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97920.4211652755423922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81300.34968072816172885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52390.22533546553988895No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42890.18447486384817405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA139200.027.7536221
GGTATCA98000.027.0083141
GTACATG99350.023.8193451
TCAACGC166100.023.2198054
ATCAACG166150.023.1842293
CAACGCA167050.023.0593225
TACATGG99750.022.8083972
TATCAAC169450.022.7046932
AACGCAG170500.022.6205846
GAGTACT91100.021.82488612-13
ACATGGG101400.021.4535773
AGTACTT94300.021.13465712-13
GTACTTT95050.020.56316214-15
AGAGTAC145300.020.30490310-11
ACGCAGA192500.019.8920367
CGCAGAG192950.019.7225328
GTCCTAC5800.018.8437881
GCAGAGT204600.018.2512179
CATGGGG44800.018.1296864
TACTTTT108750.018.12554214-15
Produced by FastQC (version 0.11.5)