##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937411_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2324978 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.206638084317355 33.0 31.0 34.0 31.0 34.0 2 32.4023964957948 34.0 31.0 34.0 31.0 34.0 3 32.47260791284907 34.0 31.0 34.0 31.0 34.0 4 36.10426163172297 37.0 35.0 37.0 35.0 37.0 5 36.03307515167886 37.0 35.0 37.0 35.0 37.0 6 36.07011206127542 37.0 35.0 37.0 35.0 37.0 7 36.01011106341652 37.0 35.0 37.0 35.0 37.0 8 35.98782999236982 37.0 35.0 37.0 35.0 37.0 9 37.682303660507756 39.0 37.0 39.0 35.0 39.0 10-11 37.5979220018426 39.0 37.0 39.0 35.0 39.0 12-13 37.493608326616425 39.0 37.0 39.0 35.0 39.0 14-15 38.72540406833957 40.0 38.0 41.0 35.0 41.0 16-17 38.684036365075286 40.0 38.0 41.0 34.5 41.0 18-19 38.667224593092925 40.0 38.0 41.0 34.0 41.0 20-21 38.56365178509216 40.0 38.0 41.0 34.0 41.0 22-23 38.50859255442417 40.0 38.0 41.0 34.0 41.0 24-25 38.54233222852001 40.0 38.0 41.0 34.0 41.0 26-27 38.39368609079312 40.0 38.0 41.0 34.0 41.0 28-29 38.249381069412266 40.0 38.0 41.0 34.0 41.0 30-31 38.296488181823655 40.0 38.0 41.0 34.0 41.0 32-33 38.19780058133884 40.0 38.0 41.0 34.0 41.0 34-35 38.07133637393558 40.0 37.5 41.0 33.5 41.0 36-37 37.90708987353859 40.0 37.0 41.0 33.0 41.0 38-39 37.798108197152835 40.0 37.0 41.0 33.0 41.0 40-41 37.617871222867485 40.0 37.0 41.0 33.0 41.0 42-43 37.4232577684606 40.0 37.0 41.0 32.5 41.0 44-45 37.13180468804436 40.0 36.5 41.0 31.5 41.0 46-47 36.78669583110033 39.5 36.0 41.0 31.0 41.0 48-49 36.46243405313943 39.0 36.0 41.0 30.0 41.0 50-51 36.21359298883688 39.0 36.0 41.0 30.0 41.0 52-53 35.984354260556444 39.0 35.0 40.0 29.0 41.0 54-55 35.77006879204878 39.0 35.0 40.0 29.0 41.0 56-57 35.47219393043719 38.0 35.0 40.0 28.0 41.0 58-59 35.21310975845793 38.0 34.5 40.0 28.0 41.0 60-61 34.867858534575376 38.0 34.0 40.0 27.0 41.0 62-63 34.49905784054731 37.5 34.0 40.0 26.0 41.0 64-65 34.086139524761094 37.0 33.5 39.5 26.0 41.0 66-67 33.652600368691665 36.0 33.0 39.0 25.0 40.5 68-69 33.205831410017645 36.0 32.5 39.0 24.0 40.0 70-71 32.76523433770126 35.0 32.0 38.5 23.0 40.0 72-73 32.28457925193271 35.0 31.5 37.5 22.0 39.5 74-75 31.796912917025452 35.0 31.0 37.0 20.5 39.0 76-77 30.47750946460569 33.5 29.5 35.5 20.0 38.0 78-79 31.132617598962227 34.0 31.0 36.0 20.0 38.5 80-81 30.94225837835885 34.0 31.0 36.0 19.5 37.5 82-83 30.53637109684479 34.0 30.5 35.0 18.0 37.0 84-85 30.08656963635785 34.0 30.0 35.0 15.0 36.5 86-87 29.70238557096024 34.0 30.0 35.0 8.5 36.0 88-89 29.249328810853264 34.0 29.0 35.0 4.5 36.0 90-91 28.694453452892887 34.0 29.0 35.0 2.0 35.0 92-93 28.18109870286945 33.0 28.5 35.0 2.0 35.0 94-95 27.673241424219928 33.0 27.0 35.0 2.0 35.0 96-97 26.990636900650244 33.0 26.0 34.5 2.0 35.0 98-99 25.99261885488809 32.0 24.5 34.0 2.0 35.0 100-101 22.992699715868277 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1490065269439711 1101 2 0.185781539567472 1101 3 0.12451162832480378 1101 4 0.06903647554911174 1101 5 0.032830143404147805 1101 6 0.05239279056387147 1101 7 0.04033148245518703 1101 8 0.09319557937462264 1101 9 0.1838381511599394 1101 10-11 0.21565036184677666 1101 12-13 0.15106240626555945 1101 14-15 0.2712961609188085 1101 16-17 0.16221149796789547 1101 18-19 0.1362824293890057 1101 20-21 0.12554531734431862 1101 22-23 0.07241910832384946 1101 24-25 0.08812239883695128 1101 26-27 0.09216640746962668 1101 28-29 0.08443686346690527 1101 30-31 0.1758678319436342 1101 32-33 0.17314881004534044 1101 34-35 0.2681692945497147 1101 36-37 0.4324841300021305 1101 38-39 0.3176281034456778 1101 40-41 0.29999258894536496 1101 42-43 0.3161512110799407 1101 44-45 0.26401973214306196 1101 46-47 0.37052682173077045 1101 48-49 0.20764366943453183 1101 50-51 0.19696157103467016 1101 52-53 0.2715479085633845 1101 54-55 0.21708754002149533 1101 56-57 0.254920660863597 1101 58-59 0.270555362535859 1101 60-61 0.11378625603018122 1101 62-63 0.20829295786831636 1101 64-65 0.18049539334545273 1101 66-67 0.14999335326334062 1101 68-69 0.1283919528649733 1101 70-71 0.06358842563107459 1101 72-73 0.16860478655479483 1101 74-75 0.17682347715367186 1101 76-77 0.3439988937055354 1101 78-79 0.35592903472756277 1101 80-81 0.3344689325975203 1101 82-83 0.33123098301020804 1101 84-85 0.34992527751958846 1101 86-87 0.5391420264930424 1101 88-89 0.823370582525019 1101 90-91 0.8087887307697414 1101 92-93 0.86735662366981 1101 94-95 0.9604255597128422 1101 96-97 1.2419696470929402 1101 98-99 1.5622978294854022 1101 100-101 2.2729826074834705 1102 1 0.20679331299500348 1102 2 0.2006281143200539 1102 3 0.19929004707586984 1102 4 0.17363010095838405 1102 5 0.1502054691497392 1102 6 0.12558832701397193 1102 7 0.09190612367100925 1102 8 0.10855547721486403 1102 9 0.22395040810361166 1102 10-11 0.13994255657396337 1102 12-13 0.139923057682509 1102 14-15 0.24100623805833266 1102 16-17 0.24360245950207826 1102 18-19 0.2745035429006748 1102 20-21 0.2920114509389862 1102 22-23 0.264189085530937 1102 24-25 0.22193304691291615 1102 26-27 0.21130220718898585 1102 28-29 0.07585544377293729 1102 30-31 0.1964916301187145 1102 32-33 0.296743482295156 1102 34-35 0.3040230635469001 1102 36-37 0.41640413950536725 1102 38-39 0.41880910291855145 1102 40-41 0.4242283582246813 1102 42-43 0.4375517500914583 1102 44-45 0.5528604861652369 1102 46-47 0.44738644858036736 1102 48-49 0.6150032554499347 1102 50-51 0.6508598270428578 1102 52-53 0.5770393667899967 1102 54-55 0.6079275367547581 1102 56-57 0.6800610934092006 1102 58-59 0.591510214753157 1102 60-61 0.7476960941039863 1102 62-63 0.7705873947646111 1102 64-65 0.8244649236035997 1102 66-67 0.7301890604746575 1102 68-69 0.7762607043034535 1102 70-71 0.7380764018135935 1102 72-73 0.9250435241096397 1102 74-75 0.6766150742165742 1102 76-77 0.6183723113800639 1102 78-79 0.723773359886625 1102 80-81 0.8416155125471789 1102 82-83 0.9156167251904606 1102 84-85 1.0223820860665604 1102 86-87 1.0147681633544536 1102 88-89 1.0052348496252712 1102 90-91 1.2284642844444456 1102 92-93 1.2992752373856504 1102 94-95 1.2506990004030136 1102 96-97 1.4764793767704987 1102 98-99 1.849137930160282 1102 100-101 2.5205937322059846 1103 1 0.1791697317706067 1103 2 0.1771067982791834 1103 3 0.16967459395820583 1103 4 0.16730377852189093 1103 5 0.09945499554989823 1103 6 0.05025146394503821 1103 7 0.15513469833786786 1103 8 0.05473283833271836 1103 9 0.15782518344727237 1103 10-11 0.17863380185820432 1103 12-13 0.22121254457552908 1103 14-15 0.21737692506145834 1103 16-17 0.28232058258945614 1103 18-19 0.2797910220541837 1103 20-21 0.3441958391451436 1103 22-23 0.22541882598202534 1103 24-25 0.20999768317204115 1103 26-27 0.18955838827412919 1103 28-29 0.1588631670934788 1103 30-31 0.1728862804644109 1103 32-33 0.21001982173266498 1103 34-35 0.3520706987859725 1103 36-37 0.436148476546542 1103 38-39 0.3723668497166912 1103 40-41 0.38267316967527165 1103 42-43 0.362022532624124 1103 44-45 0.41163463166157754 1103 46-47 0.4785987022679734 1103 48-49 0.4924571503026769 1103 50-51 0.3938131470992019 1103 52-53 0.4321712461313254 1103 54-55 0.41510795805622536 1103 56-57 0.49479223442052245 1103 58-59 0.3429378146948636 1103 60-61 0.41807545972307025 1103 62-63 0.5033961251932055 1103 64-65 0.6390542275332649 1103 66-67 0.6486539493203693 1103 68-69 0.6347249454912571 1103 70-71 0.6576225555351911 1103 72-73 0.5768053155314092 1103 74-75 0.645980369467626 1103 76-77 0.592612041954375 1103 78-79 0.5678947309784199 1103 80-81 0.6682386242195939 1103 82-83 0.6146096171449287 1103 84-85 0.5713048690525895 1103 86-87 0.7245120146521984 1103 88-89 0.9404434764285945 1103 90-91 1.1769605846986515 1103 92-93 1.339684233353978 1103 94-95 1.2606143191316619 1103 96-97 1.305079140375362 1103 98-99 1.6339798206737122 1103 100-101 2.3854835494045368 1104 1 0.14254209625136838 1104 2 0.14898531604293197 1104 3 0.10809134078923677 1104 4 0.09478324738745414 1104 5 -0.01965505237846088 1104 6 0.10207944050932838 1104 7 0.09714721772957091 1104 8 0.08211925324073377 1104 9 0.08949502722008873 1104 10-11 0.07062097861685146 1104 12-13 0.1090966049761306 1104 14-15 0.14874177319229176 1104 16-17 0.19134887924364108 1104 18-19 0.18055087539433856 1104 20-21 0.30024814450618464 1104 22-23 0.2250979502663597 1104 24-25 0.044527315389537137 1104 26-27 0.152437160773907 1104 28-29 0.18910030079584317 1104 30-31 0.20769671167552417 1104 32-33 0.17655968792094257 1104 34-35 0.19977915503979915 1104 36-37 0.1918458213327341 1104 38-39 0.3066537951173913 1104 40-41 0.3134776312883858 1104 42-43 0.43613270824791783 1104 44-45 0.2948330880529326 1104 46-47 0.3018195937654937 1104 48-49 0.3501446776016621 1104 50-51 0.3865238658542651 1104 52-53 0.2718240848009046 1104 54-55 0.2198604875718786 1104 56-57 0.1501301329203244 1104 58-59 0.1418386189141856 1104 60-61 0.24686108920592176 1104 62-63 0.3839155967829342 1104 64-65 0.1671994480239931 1104 66-67 0.26592649264023294 1104 68-69 0.29970319470167794 1104 70-71 0.19564338102399148 1104 72-73 0.17708765341877353 1104 74-75 0.2683096521270407 1104 76-77 0.20317926508938555 1104 78-79 0.16610873019653027 1104 80-81 0.20812855075215353 1104 82-83 0.09515832771568711 1104 84-85 0.18824049287099598 1104 86-87 0.3597634207754261 1104 88-89 0.4092857895888855 1104 90-91 0.523527803904571 1104 92-93 0.6268933742518144 1104 94-95 0.6708157766932281 1104 96-97 0.8062347840880655 1104 98-99 1.0235928445606177 1104 100-101 1.7078005863988892 1105 1 0.14327035269455024 1105 2 0.1741149003388074 1105 3 0.15348971989447335 1105 4 0.06365521558707599 1105 5 0.02976123975410161 1105 6 0.016107439476812146 1105 7 0.057735482441543695 1105 8 0.09670690229723533 1105 9 0.2033204687012926 1105 10-11 0.07897068956629028 1105 12-13 0.06262230424364645 1105 14-15 0.17331378857559798 1105 16-17 0.1812437580896642 1105 18-19 0.18177434311265017 1105 20-21 0.2360014511694004 1105 22-23 0.1127043271152317 1105 24-25 0.18738338215865014 1105 26-27 0.19084830059696145 1105 28-29 0.13740333437453245 1105 30-31 0.005004337199196129 1105 32-33 0.2115959050503946 1105 34-35 0.22116829061540244 1105 36-37 0.2913063306991148 1105 38-39 0.1867091513045338 1105 40-41 0.172751664139291 1105 42-43 0.05505687670144965 1105 44-45 0.18566272574496878 1105 46-47 0.23016103902759966 1105 48-49 0.29925315752591786 1105 50-51 0.18437034523368112 1105 52-53 0.15458481505154964 1105 54-55 0.3325668307802445 1105 56-57 0.29372229937219885 1105 58-59 0.2876340367849082 1105 60-61 0.31831773876655234 1105 62-63 0.3255515660970687 1105 64-65 0.3267713220736752 1105 66-67 0.37588754668465185 1105 68-69 0.381880620895231 1105 70-71 0.4550976686046724 1105 72-73 0.32807824681187725 1105 74-75 0.4248068209505256 1105 76-77 0.42205356296413044 1105 78-79 0.4215909216939586 1105 80-81 0.4286554425027127 1105 82-83 0.36459255524045986 1105 84-85 0.3963554968174634 1105 86-87 0.5658462491128446 1105 88-89 0.6702361168771773 1105 90-91 0.8709444116304574 1105 92-93 0.6661089508945537 1105 94-95 0.9352703236444135 1105 96-97 1.311002774848884 1105 98-99 1.5337746896819482 1105 100-101 2.216743820913617 1106 1 -0.006090594392709647 1106 2 0.10705315999896214 1106 3 0.05260429071491046 1106 4 0.07549870931966751 1106 5 0.09120195119179186 1106 6 0.10802526284532377 1106 7 0.01918093345584282 1106 8 0.011046409610074193 1106 9 0.039221072697678494 1106 10-11 -0.08694009634812261 1106 12-13 -0.17686141966893132 1106 14-15 0.003299183052746457 1106 16-17 -0.014426665917220305 1106 18-19 0.05411956733548351 1106 20-21 0.2060830100215867 1106 22-23 0.19627858892616956 1106 24-25 0.27009880000135666 1106 26-27 0.2255191206581273 1106 28-29 0.28250302074531675 1106 30-31 0.25424995458897826 1106 32-33 0.26220995261068225 1106 34-35 -0.054252864348597996 1106 36-37 0.3828327304132131 1106 38-39 0.38436502208733003 1106 40-41 0.27871143657424113 1106 42-43 0.3358980371459168 1106 44-45 0.24118713810556613 1106 46-47 0.3317247179632332 1106 48-49 0.3117626434888692 1106 50-51 0.34100436329964623 1106 52-53 0.3934360506373835 1106 54-55 0.34875302967793687 1106 56-57 0.3878985037558138 1106 58-59 0.46993422413437713 1106 60-61 0.4412915016689638 1106 62-63 0.4783378106472398 1106 64-65 0.41145666049306584 1106 66-67 0.43100555397052176 1106 68-69 0.48263911658630576 1106 70-71 0.5022194108948312 1106 72-73 0.5320709859984234 1106 74-75 0.562107724684271 1106 76-77 0.3485212109955427 1106 78-79 0.5094948139759126 1106 80-81 0.4856139853716357 1106 82-83 0.5682251955605295 1106 84-85 0.808988756551166 1106 86-87 0.7722052355407705 1106 88-89 0.9199224604136766 1106 90-91 0.9862384260899795 1106 92-93 1.1486180064821632 1106 94-95 0.7212196036373548 1106 96-97 0.9959467742997461 1106 98-99 1.1998696108715095 1106 100-101 1.834676199685326 1107 1 0.10779893973549548 1107 2 0.10810375763860947 1107 3 0.10464990530487484 1107 4 0.03203860721802698 1107 5 0.011322160724326125 1107 6 0.0773030537209749 1107 7 0.05600572269429449 1107 8 0.05707170003051942 1107 9 0.13628791453475486 1107 10-11 0.08760537680409897 1107 12-13 0.13318624050665306 1107 14-15 0.15312684140171484 1107 16-17 0.16163146702344733 1107 18-19 0.145169850239931 1107 20-21 0.09536226065063147 1107 22-23 0.1783009343520945 1107 24-25 0.03972963493588111 1107 26-27 0.07088914400452495 1107 28-29 0.019471584106618423 1107 30-31 0.09295029521740616 1107 32-33 0.11145720451873586 1107 34-35 0.06560949403746719 1107 36-37 0.018213271321350533 1107 38-39 0.025171076279320914 1107 40-41 0.06874869697242758 1107 42-43 0.12357179076078495 1107 44-45 0.09269478723259539 1107 46-47 0.126741455889416 1107 48-49 0.13123121602191645 1107 50-51 0.16921320921205307 1107 52-53 0.017403675778894012 1107 54-55 0.011285369683854185 1107 56-57 0.057836775110452265 1107 58-59 0.12067673850551586 1107 60-61 0.2683455783874962 1107 62-63 0.2358510512476144 1107 64-65 0.3124772713998567 1107 66-67 0.2315402948459564 1107 68-69 0.2777879274376005 1107 70-71 0.4737089405433679 1107 72-73 0.3487975971674331 1107 74-75 0.35877585689699387 1107 76-77 0.3224626822222767 1107 78-79 0.42088469421628005 1107 80-81 0.35852121360311173 1107 82-83 0.3449435579643847 1107 84-85 0.43459039796274723 1107 86-87 0.6220914191873987 1107 88-89 0.76526718909167 1107 90-91 0.9312946410768959 1107 92-93 0.9740712782509746 1107 94-95 0.9337647164853635 1107 96-97 1.1545722465714796 1107 98-99 1.4687728861835083 1107 100-101 2.092382529357497 1108 1 0.08610569548224589 1108 2 0.07788301628083616 1108 3 0.15106549698186456 1108 4 -0.006047578332513126 1108 5 -0.0035415090936155025 1108 6 0.07625714248536042 1108 7 0.024197286711569177 1108 8 0.024322331901942107 1108 9 -0.003543548383092343 1108 10-11 0.048317522998893025 1108 12-13 0.12090882185119511 1108 14-15 0.04112442231834024 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0.4263189949415711 2316 80-81 0.38018316975966115 2316 82-83 0.5487086270835562 2316 84-85 0.6484190002568617 2316 86-87 0.7030668614297717 2316 88-89 0.6188583519606148 2316 90-91 0.7085308473181584 2316 92-93 0.7028053260609326 2316 94-95 0.9135302917227612 2316 96-97 1.181399388503614 2316 98-99 1.4143183268298785 2316 100-101 1.532639130342222 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 7.0 4 20.0 5 51.0 6 107.0 7 230.0 8 506.0 9 1020.0 10 1730.0 11 2864.0 12 4591.0 13 6617.0 14 8556.0 15 11509.0 16 16001.0 17 22625.0 18 20521.0 19 12811.0 20 11030.0 21 11535.0 22 11920.0 23 13228.0 24 15383.0 25 18444.0 26 22607.0 27 28333.0 28 35140.0 29 44329.0 30 55837.0 31 70311.0 32 90151.0 33 119157.0 34 164327.0 35 233403.0 36 354006.0 37 514538.0 38 373499.0 39 28026.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.401175587201934 20.167387782413098 15.211762756863866 22.219673873521103 2 15.462346509234285 21.30064082354363 39.681510827849046 23.555501839373036 3 19.235407818912694 25.111334386820005 30.16325315766429 25.490004636603015 4 12.724335456077435 17.64777989297103 37.64027014449169 31.987614506459845 5 13.282983906764526 38.10633509853107 34.39421545046952 14.216465544234891 6 31.17139172929808 37.389257016625535 17.41663792087495 14.022713333201434 7 28.09802931468599 33.3653909843448 20.802347377050452 17.734232323918764 8 26.281797075069097 35.31990410231839 19.77038062295643 18.627918199656083 9 26.249022571396374 16.95844003685196 20.52028019189859 36.27225719985307 10-11 24.566963644387172 27.412581968517554 27.45729206900022 20.563162318095053 12-13 25.669614005627423 24.706203434134146 28.070333531122554 21.55384902911588 14-15 22.34092107538222 26.106569610551155 26.648467211302645 24.90404210276398 16-17 21.554203953757842 29.524472919743754 27.226967308937976 21.694355817560425 18-19 21.43291248347296 27.842457003894232 29.853916897278165 20.87071361535464 20-21 22.294968445931197 26.828732751177164 30.006892539820278 20.86940626307136 22-23 21.987224759415835 26.931038991537942 29.777725781796377 21.304010467249842 24-25 21.863652443948382 27.00161614467237 29.96747280350025 21.167258607879 26-27 21.638192182445447 27.335354311302577 29.882251079271917 21.144202426980055 28-29 21.416959599467937 27.55107461264588 29.95495519714225 21.07701059074393 30-31 21.730056940384962 27.123146028850627 29.839217660573198 21.307579370191213 32-33 21.527159193402774 27.333904808520685 29.746014691758244 21.3929213063183 34-35 21.916895557721404 27.265698858225758 29.72782968268947 21.089575901363368 36-37 21.98532631276511 27.033976235474057 29.643119203708597 21.33757824805224 38-39 21.620914262414527 27.45045329461182 29.704517634145354 21.224114808828297 40-41 21.891411445613677 27.295914197897787 29.614774849482444 21.19789950700609 42-43 21.703794974059043 27.457928074196538 29.27997845834811 21.55829849339631 44-45 21.828830208285844 27.489378394118134 29.0886838499117 21.593107547684323 46-47 22.065434597660712 27.827188042209432 28.67386272042144 21.433514639708417 48-49 22.114510330850443 27.858629199932217 28.4963556644407 21.530504804776648 50-51 21.99584254130577 28.005555321383685 28.373666331466364 21.624935805844185 52-53 22.36028090594425 28.21915480902503 27.917173392000567 21.503390893030147 54-55 22.080832592824535 27.990910021514182 28.111599335563604 21.81665805009768 56-57 22.068208817459777 28.254546924745092 28.022351179236964 21.654893078558164 58-59 22.349652340796343 27.89832850031269 28.032136218063137 21.71988294082783 60-61 22.156811806391286 27.993813274792277 28.422118402840802 21.427256515975635 62-63 22.041584909620653 28.019146847841142 28.422118402840802 21.517149839697407 64-65 21.889389921108933 28.099577716434304 28.25267593929921 21.75835642315755 66-67 21.955627107009185 28.178804272556558 27.979770131158233 21.885798489276027 68-69 21.992036053674486 27.9724582340134 28.137771626226137 21.897734086085975 70-71 22.0502731638751 27.967683995289416 28.110739112370098 21.87130372846539 72-73 22.078992332167367 27.848178849427253 28.13280587189741 21.94002294650797 74-75 21.887798508200937 28.327192773436995 27.79200491359488 21.993003804767184 76-77 21.884852243763167 27.985533626554748 28.05071703904295 22.07889709063914 78-79 21.958981977463875 28.00506069304742 27.96187748873323 22.07407984075548 80-81 21.95317547090768 28.222503610786852 27.849016205744743 21.97530471256072 82-83 21.932889872318757 28.201273177992224 27.86331175527249 22.002525194416528 84-85 21.92415915188729 28.134529676460897 27.75282648322089 22.188484688430922 86-87 21.7538832625513 28.03714701816533 27.92759759447186 22.281372124811504 88-89 21.802507378564442 28.013576902663168 27.66213271695474 22.521783001817653 90-91 21.835172289495045 27.913190888252178 27.77605957383476 22.47557724841802 92-93 21.838134944035332 27.83136318259024 27.847578592046535 22.482923281327892 94-95 21.63620472967916 27.782671491945298 27.877876693886993 22.70324708448854 96-97 21.610333517134357 27.836048341102583 27.926694360118674 22.626923781644383 98-99 21.530532586371898 28.055957590941837 27.439585371885723 22.973924450800546 100-101 21.399176423675947 27.904659404776194 27.791614456504583 22.90454971504328 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 319.0 1 232.0 2 182.5 3 242.5 4 241.5 5 245.0 6 417.5 7 879.0 8 1520.0 9 2021.5 10 2102.5 11 2115.5 12 2371.5 13 2592.0 14 2831.0 15 3100.5 16 3307.0 17 3541.0 18 3800.0 19 4074.0 20 4442.0 21 5091.0 22 5988.5 23 7060.5 24 8567.5 25 10854.5 26 13820.0 27 17314.0 28 20967.0 29 25412.0 30 30605.5 31 35401.5 32 40437.0 33 45969.5 34 51829.0 35 56640.0 36 62142.0 37 68250.0 38 71885.0 39 74246.0 40 76065.0 41 78366.5 42 80811.0 43 84679.5 44 89017.0 45 90410.0 46 88872.0 47 90304.0 48 97139.5 49 96273.0 50 89009.0 51 82996.0 52 76671.0 53 71732.0 54 67280.0 55 60940.0 56 53575.5 57 46885.5 58 41575.5 59 36753.5 60 31071.0 61 24945.5 62 19579.0 63 15080.0 64 11201.5 65 8529.5 66 6322.5 67 4840.0 68 3433.5 69 2046.5 70 1452.0 71 1089.0 72 865.0 73 704.5 74 516.5 75 387.0 76 264.5 77 151.0 78 89.0 79 58.0 80 38.5 81 20.0 82 13.5 83 9.5 84 5.5 85 2.5 86 1.5 87 1.5 88 1.5 89 1.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04443052794478056 2 4.30111596754894E-5 3 0.0 4 0.0 5 0.0011182901515627245 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004838755463492558 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.15055798377447E-5 22-23 5.161339161058728E-4 24-25 2.15055798377447E-5 26-27 0.0013333459499401715 28-29 0.003032286757122003 30-31 1.0752789918872351E-4 32-33 4.30111596754894E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.007720503161750348 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.15055798377447E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.365613782151917E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.6559485724165994E-4 84-85 1.720446387019576E-4 86-87 0.0 88-89 0.0 90-91 0.008559220775422392 92-93 0.0012473236305891927 94-95 0.0 96-97 0.0 98-99 1.290334790264682E-4 100-101 0.010279667162441967 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2324978.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.955495645935805 #Duplication Level Percentage of deduplicated Percentage of total 1 75.50558833744276 41.494470311221576 2 13.765515782245668 15.129814852705248 3 4.631500475958814 7.635792127221126 4 2.068650028417663 4.547347505186875 5 1.1226026254042543 3.08465918462598 6 0.6707826783804907 2.2117916736664927 7 0.42444970985597125 1.6328090927335979 8 0.3051822000651898 1.341715125351972 9 0.20924369943022364 1.0349182091679443 >10 1.1557454410247898 11.640819181197362 >50 0.09460723184135915 3.587001908933888 >100 0.04330070121000231 4.3272202377715425 >500 0.001966033835330983 0.7328433113068054 >1k 6.291308273055499E-4 0.5978310742054983 >5k 2.3592406023958116E-4 1.0009662047042251 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9792 0.4211652755423922 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8130 0.34968072816172885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5239 0.22533546553988895 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4289 0.18447486384817405 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 8.60223193509788E-5 7 0.0 0.0 0.0 0.0 8.60223193509788E-5 8 0.0 0.0 0.0 0.0 8.60223193509788E-5 9 0.0 0.0 0.0 1.290334790264682E-4 1.290334790264682E-4 10-11 0.0 0.0 0.0 1.290334790264682E-4 1.290334790264682E-4 12-13 0.0 0.0 0.0 1.290334790264682E-4 1.290334790264682E-4 14-15 0.0 0.0 0.0 1.5053905886421292E-4 1.290334790264682E-4 16-17 2.15055798377447E-5 0.0 0.0 2.1505579837744702E-4 1.290334790264682E-4 18-19 4.30111596754894E-5 0.0 0.0 3.440892774039152E-4 1.290334790264682E-4 20-21 4.30111596754894E-5 0.0 0.0 4.946283362681281E-4 1.290334790264682E-4 22-23 4.30111596754894E-5 0.0 0.0 6.881785548078304E-4 1.290334790264682E-4 24-25 8.60223193509788E-5 0.0 0.0 0.001010762252374001 1.290334790264682E-4 26-27 8.60223193509788E-5 0.0 0.0 0.0015484017483176184 1.290334790264682E-4 28-29 8.60223193509788E-5 0.0 0.0 0.002817230958744556 1.290334790264682E-4 30-31 8.60223193509788E-5 0.0 0.0 0.005785000976353325 1.290334790264682E-4 32-33 8.60223193509788E-5 0.0 0.0 0.009311916069743456 1.720446387019576E-4 34-35 8.60223193509788E-5 0.0 0.0 0.013290448339726226 1.935502185397023E-4 36-37 8.60223193509788E-5 0.0 0.0 0.01789264242500359 2.365613782151917E-4 38-39 1.720446387019576E-4 0.0 0.0 0.022322791871578998 2.580669580529364E-4 40-41 1.720446387019576E-4 0.0 0.0 0.025742179065780406 2.580669580529364E-4 42-43 1.935502185397023E-4 0.0 0.0 0.03068846242846169 2.580669580529364E-4 44-45 2.1505579837744702E-4 0.0 0.0 0.03492506165649739 2.580669580529364E-4 46-47 2.580669580529364E-4 0.0 0.0 0.04316169873435362 2.580669580529364E-4 48-49 3.440892774039152E-4 0.0 0.0 0.05464567836770928 2.580669580529364E-4 50-51 3.440892774039152E-4 0.0 0.0 0.06468878415193606 2.580669580529364E-4 52-53 3.440892774039152E-4 0.0 0.0 0.08565672449373715 2.580669580529364E-4 54-55 3.440892774039152E-4 0.0 0.0 0.12808723351360743 2.580669580529364E-4 56-57 3.871004370794046E-4 0.0 0.0 0.19757176196936058 2.580669580529364E-4 58-59 3.871004370794046E-4 0.0 0.0 0.27054019435882837 2.580669580529364E-4 60-61 3.871004370794046E-4 0.0 0.0 0.334927900393036 2.580669580529364E-4 62-63 3.871004370794046E-4 0.0 0.0 0.41854159480218733 2.580669580529364E-4 64-65 3.871004370794046E-4 0.0 0.0 0.5167790835010052 2.580669580529364E-4 66-67 4.3011159675489404E-4 0.0 0.0 0.6700493510046117 2.580669580529364E-4 68-69 5.376394959436175E-4 0.0 0.0 0.860567282787192 2.580669580529364E-4 70-71 5.80650655619107E-4 0.0 0.0 1.0418163096597044 2.580669580529364E-4 72-73 6.236618152945963E-4 0.0 0.0 1.2366568629896713 2.580669580529364E-4 74-75 6.45167395132341E-4 0.0 0.0 1.4509384604929596 2.580669580529364E-4 76-77 6.45167395132341E-4 0.0 0.0 1.6988332792826428 2.580669580529364E-4 78-79 6.45167395132341E-4 0.0 0.0 1.9237386332257769 2.580669580529364E-4 80-81 6.45167395132341E-4 0.0 0.0 2.1660204956778086 2.580669580529364E-4 82-83 6.881785548078304E-4 0.0 0.0 2.445636044728165 2.580669580529364E-4 84-85 7.096841346455752E-4 0.0 0.0 2.818822371652549 2.580669580529364E-4 86-87 7.311897144833199E-4 0.0 0.0 3.2396005467578615 2.580669580529364E-4 88-89 7.311897144833199E-4 0.0 0.0 3.7531537932832055 2.580669580529364E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13920 0.0 27.753622 1 GGTATCA 9800 0.0 27.008314 1 GTACATG 9935 0.0 23.819345 1 TCAACGC 16610 0.0 23.219805 4 ATCAACG 16615 0.0 23.184229 3 CAACGCA 16705 0.0 23.059322 5 TACATGG 9975 0.0 22.808397 2 TATCAAC 16945 0.0 22.704693 2 AACGCAG 17050 0.0 22.620584 6 GAGTACT 9110 0.0 21.824886 12-13 ACATGGG 10140 0.0 21.453577 3 AGTACTT 9430 0.0 21.134657 12-13 GTACTTT 9505 0.0 20.563162 14-15 AGAGTAC 14530 0.0 20.304903 10-11 ACGCAGA 19250 0.0 19.892036 7 CGCAGAG 19295 0.0 19.722532 8 GTCCTAC 580 0.0 18.843788 1 GCAGAGT 20460 0.0 18.251217 9 CATGGGG 4480 0.0 18.129686 4 TACTTTT 10875 0.0 18.125542 14-15 >>END_MODULE