FastQCFastQC Report
Thu 26 May 2016
SRR937410_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937410_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314103
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89870.38835782158356824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69770.3014991121829927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45750.19770079378489203No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31150.13460939292676255No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30110.1301152109478273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA119950.030.8807071
GGTATCA82050.029.4977721
GTACATG95350.027.5276031
TACATGG94800.026.9250182
ACATGGG93450.026.1949733
TATCAAC145250.025.0015682
ATCAACG144200.024.9858423
TCAACGC144950.024.728074
CAACGCA145050.024.7104855
AACGCAG147600.024.3321726
TATACCG1302.9274868E-421.9399475
GAGTACT82900.021.65933612-13
ACGCAGA164550.021.5943347
CGCAGAG165550.021.4060388
CATGGGG42500.021.1402424
AGAGTAC129550.020.58895510-11
AGTACTT86750.020.34216712-13
GTACTTT88000.020.32267214-15
CATGGGA52650.020.0443364
GCAGAGT172600.019.8711539
Produced by FastQC (version 0.11.5)