##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937410_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2314103 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.663973902630953 33.0 31.0 34.0 30.0 34.0 2 31.770524475358272 33.0 31.0 34.0 30.0 34.0 3 31.80499787606688 33.0 31.0 34.0 30.0 34.0 4 35.429295498082844 37.0 35.0 37.0 33.0 37.0 5 35.44206977822508 37.0 35.0 37.0 33.0 37.0 6 35.480096175494346 37.0 35.0 37.0 33.0 37.0 7 35.419632142562364 37.0 35.0 37.0 33.0 37.0 8 35.40860497566444 37.0 35.0 37.0 33.0 37.0 9 37.022942799002465 39.0 37.0 39.0 33.0 39.0 10-11 36.894432745647016 39.0 37.0 39.0 33.0 39.0 12-13 36.7659306867499 39.0 37.0 39.0 33.0 39.0 14-15 37.92556489490744 40.0 38.0 41.0 33.0 41.0 16-17 37.88509997178173 40.0 38.0 41.0 33.0 41.0 18-19 37.860236342116146 40.0 38.0 41.0 33.0 41.0 20-21 37.80075778822291 40.0 38.0 41.0 32.5 41.0 22-23 37.65148634265631 40.0 37.0 41.0 32.0 41.0 24-25 37.49068732031375 40.0 37.0 41.0 32.0 41.0 26-27 37.33756686716192 39.5 37.0 41.0 32.0 41.0 28-29 37.394322119629074 40.0 37.0 41.0 32.0 41.0 30-31 37.25027148748349 39.5 37.0 41.0 31.5 41.0 32-33 37.05492862677245 39.0 36.0 41.0 31.0 41.0 34-35 36.93742391760436 39.0 36.0 41.0 31.0 41.0 36-37 36.942727700538825 39.0 36.0 41.0 31.0 41.0 38-39 36.85771225394894 40.0 36.0 41.0 30.5 41.0 40-41 36.68812559337246 39.5 36.0 41.0 30.0 41.0 42-43 36.448384752104815 39.0 36.0 41.0 30.0 41.0 44-45 36.225212749821424 39.0 35.0 41.0 30.0 41.0 46-47 35.88492690256224 39.0 35.0 41.0 28.5 41.0 48-49 35.5709244575544 39.0 35.0 41.0 27.0 41.0 50-51 34.699054017906725 38.0 34.0 39.5 25.5 40.5 52-53 34.799131023986405 38.0 34.5 40.0 26.0 40.5 54-55 35.130317017003996 39.0 35.0 40.0 26.0 41.0 56-57 34.93649526403968 38.5 34.5 40.0 25.5 41.0 58-59 34.70926855891894 38.0 34.0 40.0 25.0 41.0 60-61 34.43225755292656 38.0 34.0 40.0 24.5 41.0 62-63 34.10928618994055 38.0 34.0 40.0 23.0 41.0 64-65 33.75931300378592 37.0 33.5 40.0 22.0 41.0 66-67 33.39594045727438 37.0 33.0 39.5 22.0 41.0 68-69 32.93292217329998 36.0 32.5 39.0 20.5 41.0 70-71 32.49547816151657 36.0 32.0 39.0 19.0 40.0 72-73 32.10702008510425 35.0 31.5 38.0 18.0 40.0 74-75 31.65117931224323 35.0 31.0 37.5 15.5 39.5 76-77 31.20994938427546 35.0 31.0 37.0 12.0 39.0 78-79 30.726988599902427 35.0 30.5 36.5 7.5 39.0 80-81 30.23779645936244 34.0 30.0 36.0 6.5 37.5 82-83 29.75724978533799 34.0 29.5 35.5 2.0 37.0 84-85 29.3082440582809 34.0 29.0 35.0 2.0 37.0 86-87 28.88135705281917 34.0 29.0 35.0 2.0 36.0 88-89 28.47376261990067 34.0 29.0 35.0 2.0 36.0 90-91 28.051637070605757 34.0 27.5 35.0 2.0 35.5 92-93 27.601170518339075 33.5 27.0 35.0 2.0 35.0 94-95 27.06807389299439 33.0 25.5 35.0 2.0 35.0 96-97 26.433398599803034 33.0 25.0 35.0 2.0 35.0 98-99 25.667705154005677 32.0 23.5 34.5 2.0 35.0 100-101 23.727873175913086 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0016010475232270949 1101 2 0.0792744960893792 1101 3 0.11942271300371488 1101 4 0.06397045783905497 1101 5 -0.05139944902081339 1101 6 0.04223719155073269 1101 7 -0.07775255482025756 1101 8 0.01507862219594358 1101 9 0.11456476787304837 1101 10-11 0.0758676647054557 1101 12-13 0.09504348059115131 1101 14-15 0.0932874612136132 1101 16-17 0.2340679533624126 1101 18-19 0.17675024516749716 1101 20-21 0.11692448161072377 1101 22-23 0.23988586824329872 1101 24-25 0.04171501937367594 1101 26-27 0.10959662422946082 1101 28-29 0.06022468913439383 1101 30-31 0.14132324371692562 1101 32-33 0.23644909334856123 1101 34-35 0.12628827890720373 1101 36-37 0.1611291118845557 1101 38-39 0.11049748226960787 1101 40-41 -0.010763413221880569 1101 42-43 0.1489595511148778 1101 44-45 0.17545596383227746 1101 46-47 0.22829532100556804 1101 48-49 0.3906089295399866 1101 50-51 0.31399266423489536 1101 52-53 0.24117857592973735 1101 54-55 0.43836997739231975 1101 56-57 0.34758170578571423 1101 58-59 0.4088663328734583 1101 60-61 0.2913341979739741 1101 62-63 0.4562379114055233 1101 64-65 0.40561660872282346 1101 66-67 0.3713492495502564 1101 68-69 0.2914731195959135 1101 70-71 0.3523273175885109 1101 72-73 0.24306559555924423 1101 74-75 0.3368639497049166 1101 76-77 0.23090011072207162 1101 78-79 0.23737601010999754 1101 80-81 0.28659258495983764 1101 82-83 0.14466870811625654 1101 84-85 0.4547452031441921 1101 86-87 0.5353760289909353 1101 88-89 0.5263704466510966 1101 90-91 0.6126307613141364 1101 92-93 0.5801892798108064 1101 94-95 0.789316805147326 1101 96-97 0.7864324846246618 1101 98-99 0.8353198520694995 1101 100-101 1.2073719873358009 1102 1 0.11449039103793268 1102 2 0.13740893062747261 1102 3 0.2320906369072091 1102 4 0.16926979526402164 1102 5 0.15707876121275177 1102 6 0.07939681853305558 1102 7 0.191251084992615 1102 8 0.038104297329311976 1102 9 0.1790500725144426 1102 10-11 0.2936584778963436 1102 12-13 0.22339480251393695 1102 14-15 0.3255085047763089 1102 16-17 0.42902265916058013 1102 18-19 0.3523654633457909 1102 20-21 0.3501269697048528 1102 22-23 0.39049182552530937 1102 24-25 0.39823889985760985 1102 26-27 0.30506439771354366 1102 28-29 0.2802079493627758 1102 30-31 0.21469585166951788 1102 32-33 0.24223701321093927 1102 34-35 0.28942661424706984 1102 36-37 0.33836891577805517 1102 38-39 0.3728695486000788 1102 40-41 0.4901732001525403 1102 42-43 0.5198328396030263 1102 44-45 0.17202816935682108 1102 46-47 0.34519308912956603 1102 48-49 0.20561389674799813 1102 50-51 0.16401223840204437 1102 52-53 0.2744696038050378 1102 54-55 0.3031557700548859 1102 56-57 0.2441833777984499 1102 58-59 0.3609014148323979 1102 60-61 0.35451020832373104 1102 62-63 0.14145506539177433 1102 64-65 0.19366268589134705 1102 66-67 0.24921086650275015 1102 68-69 0.26915386843759137 1102 70-71 0.2284536390201879 1102 72-73 0.26445517901535 1102 74-75 0.38716317487659424 1102 76-77 0.5817871923330173 1102 78-79 0.4837117119877128 1102 80-81 0.40817861167050395 1102 82-83 0.4723689609667865 1102 84-85 0.5416524005444074 1102 86-87 0.5420445759001815 1102 88-89 0.6616675811052808 1102 90-91 0.6230882074957016 1102 92-93 0.5292963750605288 1102 94-95 0.7211405422276727 1102 96-97 1.019265378767006 1102 98-99 1.1576501180817296 1102 100-101 1.5334392354745 1103 1 0.10121642457728797 1103 2 0.025355807542691622 1103 3 0.01163069305219011 1103 4 -0.08692713612605729 1103 5 -0.07679242722972646 1103 6 -0.03908086171512082 1103 7 0.011245880849834577 1103 8 -0.01582574023429828 1103 9 0.007582295670097494 1103 10-11 0.06947013112291245 1103 12-13 7.797547898178436E-4 1103 14-15 0.01535897664768271 1103 16-17 -0.041334363081588776 1103 18-19 0.03621556450704588 1103 20-21 -0.15287377038401218 1103 22-23 -0.25879547466511355 1103 24-25 -0.1270855861371416 1103 26-27 0.12353404541615731 1103 28-29 -0.004597196318457009 1103 30-31 -0.11240538015019297 1103 32-33 -0.11020009513774909 1103 34-35 -0.02843637656106779 1103 36-37 0.061899200323956904 1103 38-39 0.029402633886036256 1103 40-41 0.02814472943826729 1103 42-43 0.04840466335625848 1103 44-45 0.08242734249279238 1103 46-47 0.050189199336088564 1103 48-49 0.14092860611407332 1103 50-51 0.04763130402616156 1103 52-53 0.029560617871752015 1103 54-55 -0.02207992894447841 1103 56-57 -0.05239063057048554 1103 58-59 0.09463451153847302 1103 60-61 0.15170034104416175 1103 62-63 -0.14506080539459276 1103 64-65 -0.07749586013802201 1103 66-67 0.017448184289328594 1103 68-69 0.07993801204471396 1103 70-71 0.18643048191617595 1103 72-73 0.0715260084636995 1103 74-75 0.17300537692425522 1103 76-77 0.13350288045663916 1103 78-79 0.18157120871249077 1103 80-81 0.07847042680242922 1103 82-83 0.22793985676129935 1103 84-85 0.21420701186905333 1103 86-87 0.41742288586739207 1103 88-89 0.4964322755971011 1103 90-91 0.5046179236983228 1103 92-93 0.3815475366361447 1103 94-95 0.49809790568290424 1103 96-97 0.7680299126079113 1103 98-99 0.7952898573775506 1103 100-101 1.0082672642952133 1104 1 0.14602200176926416 1104 2 0.1639332329595966 1104 3 0.14814133504395244 1104 4 0.10594738057361042 1104 5 0.19959363002493546 1104 6 0.1451819351861232 1104 7 0.06799862463496709 1104 8 0.15563018441777388 1104 9 0.22665474027824928 1104 10-11 0.2740904148389802 1104 12-13 0.23992675903160432 1104 14-15 0.3954986615874816 1104 16-17 0.3275614774856166 1104 18-19 0.36751266282816886 1104 20-21 0.38392560543126564 1104 22-23 0.2683399473780739 1104 24-25 0.3485669866159853 1104 26-27 0.3731550740247158 1104 28-29 0.2549215913953944 1104 30-31 0.3536307477226259 1104 32-33 0.1641373611071586 1104 34-35 0.2774974986864436 1104 36-37 0.317509575210174 1104 38-39 0.3260749146178341 1104 40-41 0.3805458933474597 1104 42-43 0.5439490917949641 1104 44-45 0.4390953620875706 1104 46-47 0.4744704367256958 1104 48-49 0.5989432828424626 1104 50-51 0.544138594001673 1104 52-53 0.5085443850805262 1104 54-55 0.6441646075329643 1104 56-57 0.6613526800602472 1104 58-59 0.6251233428431959 1104 60-61 0.6885165795833714 1104 62-63 0.5545761035009491 1104 64-65 0.663278616912244 1104 66-67 0.8501212808837053 1104 68-69 0.9184976656169184 1104 70-71 0.8085828810565516 1104 72-73 0.8093726767413614 1104 74-75 0.6311057602799544 1104 76-77 0.720238280563624 1104 78-79 0.8624374864318796 1104 80-81 1.0005883233433153 1104 82-83 1.0214807296580055 1104 84-85 1.1304868134343842 1104 86-87 1.2398362613213614 1104 88-89 1.0156635524015023 1104 90-91 1.1527753558485863 1104 92-93 1.3650399563285198 1104 94-95 1.3381031345936485 1104 96-97 1.5007063964103118 1104 98-99 1.7363104275546348 1104 100-101 1.9401093805172138 1105 1 0.23546611732692568 1105 2 0.20966568027193233 1105 3 0.18095272505105342 1105 4 0.09426951531195016 1105 5 0.21851338493850392 1105 6 0.22852094181648397 1105 7 0.30277115009080546 1105 8 0.24504248017908026 1105 9 0.18356200407176004 1105 10-11 0.27767724588326104 1105 12-13 0.1769891258324492 1105 14-15 0.3002572011328297 1105 16-17 0.1927303444048647 1105 18-19 0.36469298487411095 1105 20-21 0.24978288994464748 1105 22-23 0.3505034715315176 1105 24-25 0.3593501976034261 1105 26-27 0.36006788778271215 1105 28-29 0.5009025291173899 1105 30-31 0.3755621353617471 1105 32-33 0.32484183659850885 1105 34-35 0.4592763733016483 1105 36-37 0.35134667265274544 1105 38-39 0.4508436660978248 1105 40-41 0.3404427380076527 1105 42-43 0.4956993285934459 1105 44-45 0.4550017082869573 1105 46-47 0.4586702058471772 1105 48-49 0.38133354154480514 1105 50-51 0.24375490645726927 1105 52-53 0.21884245517288292 1105 54-55 0.23801458089313599 1105 56-57 0.2182237580853439 1105 58-59 0.4009558375152267 1105 60-61 0.35632108763451953 1105 62-63 0.24572235616493288 1105 64-65 0.2840961636799477 1105 66-67 0.354428053320369 1105 68-69 0.35069323156529464 1105 70-71 0.3933715384091485 1105 72-73 0.44465017496537484 1105 74-75 0.3919206430647044 1105 76-77 0.42695921354965094 1105 78-79 0.32956756279939015 1105 80-81 0.5307609642384143 1105 82-83 0.5956597789120899 1105 84-85 0.6527602268845385 1105 86-87 0.6792899968174559 1105 88-89 0.5634907783072549 1105 90-91 0.7818239439376207 1105 92-93 0.7456109366742467 1105 94-95 0.8823282855526813 1105 96-97 1.1338313224107885 1105 98-99 1.453546767242063 1105 100-101 1.676551705286336 1106 1 0.0670629224984225 1106 2 0.06851013337885092 1106 3 -0.013169245282679043 1106 4 0.06184127510697124 1106 5 -0.0981608749275793 1106 6 -0.0029814965344527877 1106 7 0.05385242980884186 1106 8 -0.0832832396521681 1106 9 0.006863137366920569 1106 10-11 -0.31084963282451383 1106 12-13 -0.27243683641269456 1106 14-15 -0.18421775146043018 1106 16-17 -0.26548992334677735 1106 18-19 -0.05237173598445111 1106 20-21 0.01914120368589778 1106 22-23 0.019827757994100637 1106 24-25 0.025413468851191112 1106 26-27 -0.08879820797280757 1106 28-29 0.13491299717519922 1106 30-31 0.10980640899303751 1106 32-33 0.18856713332206 1106 34-35 0.0948549385524089 1106 36-37 0.13753119145911086 1106 38-39 0.04647737340637548 1106 40-41 -0.013490336263586755 1106 42-43 -0.0566590704190304 1106 44-45 0.09209607104698136 1106 46-47 0.2738478339265882 1106 48-49 0.24836070245972763 1106 50-51 0.1507227835692575 1106 52-53 -0.18619025243209109 1106 54-55 0.13117915841483097 1106 56-57 0.01600938622972592 1106 58-59 -0.015052819544209228 1106 60-61 -0.018471276936509184 1106 62-63 0.13047699837093774 1106 64-65 0.2397474124973158 1106 66-67 0.33437307368394187 1106 68-69 0.14658543249678502 1106 70-71 0.2136441626919563 1106 72-73 0.21974334392827188 1106 74-75 0.06320153865618394 1106 76-77 0.3364725584961157 1106 78-79 0.30506987121322027 1106 80-81 0.37128396107525674 1106 82-83 0.2452755305373202 1106 84-85 0.12165685785910796 1106 86-87 0.15491111646922562 1106 88-89 0.2386693809149314 1106 90-91 0.36986859357440593 1106 92-93 0.6470633086354418 1106 94-95 0.7785557242081573 1106 96-97 0.8336097796513293 1106 98-99 1.0983429893208125 1106 100-101 0.9210155320152111 1107 1 0.06435227240073971 1107 2 0.041110187520239094 1107 3 0.09393151212380246 1107 4 0.03896295858898213 1107 5 -0.0450344605196662 1107 6 0.11177586268362205 1107 7 0.09046249867439116 1107 8 0.04741413864429944 1107 9 0.16409731461837396 1107 10-11 0.25013086338559276 1107 12-13 0.19786507343187054 1107 14-15 0.24131859560017688 1107 16-17 0.36281039161859496 1107 18-19 0.19301174401761045 1107 20-21 0.08349282477640685 1107 22-23 0.30778876545358 1107 24-25 0.3748313952360931 1107 26-27 0.33924522186969597 1107 28-29 0.27704613199011163 1107 30-31 0.3005120123400644 1107 32-33 0.29504698355953707 1107 34-35 0.4052513201030834 1107 36-37 0.4166288744082962 1107 38-39 0.4236646030575315 1107 40-41 0.2677462358132061 1107 42-43 0.20710061200879437 1107 44-45 0.302866035325124 1107 46-47 0.32689171136652817 1107 48-49 0.41932887004593766 1107 50-51 0.33768029547176326 1107 52-53 0.34320024876245725 1107 54-55 0.33393292109794714 1107 56-57 0.3037082806282356 1107 58-59 0.3259408754192066 1107 60-61 0.4200594504487256 1107 62-63 0.4923736728293804 1107 64-65 0.5111110592777663 1107 66-67 0.448574964478766 1107 68-69 0.42313870303756573 1107 70-71 0.46040588473179866 1107 72-73 0.4082753245863415 1107 74-75 0.4773877098288999 1107 76-77 0.41856040719242316 1107 78-79 0.5273248277282363 1107 80-81 0.5872097107472563 1107 82-83 0.6251131636707008 1107 84-85 0.6168664910154078 1107 86-87 0.6251311159822386 1107 88-89 0.7237741437813874 1107 90-91 0.9253932350667604 1107 92-93 1.0036550582329653 1107 94-95 0.8974525540724336 1107 96-97 1.0984265977133525 1107 98-99 1.161033218232884 1107 100-101 1.1371811939151435 1108 1 0.14078146019062032 1108 2 0.12642663126956322 1108 3 0.08159870012941184 1108 4 0.10033174297038272 1108 5 0.028987354818482913 1108 6 0.12460686796891451 1108 7 0.18950993152952833 1108 8 0.20325897738877075 1108 9 0.1541819872534731 1108 10-11 0.21991904696880482 1108 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8321.0 3 1803.0 4 1710.0 5 1840.0 6 2082.0 7 2468.0 8 3002.0 9 3657.0 10 4817.0 11 6257.0 12 8033.0 13 10559.0 14 11960.0 15 14755.0 16 18351.0 17 23490.0 18 20128.0 19 14057.0 20 13873.0 21 14570.0 22 15941.0 23 17375.0 24 19888.0 25 22503.0 26 26336.0 27 30958.0 28 36725.0 29 44679.0 30 54841.0 31 68068.0 32 86651.0 33 113055.0 34 152090.0 35 214463.0 36 317519.0 37 457098.0 38 395863.0 39 54306.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31041620975453 19.139511276663804 14.915519293467188 22.63455322011448 2 14.923113050591319 20.231631358575846 41.03296095775507 23.812294633077766 3 18.874644738666298 24.73749653403577 30.26947871897962 26.118380008318315 4 12.140488702698276 16.75455551035104 38.41871137667681 32.68624441027387 5 13.139339205063413 37.49618718825931 34.9050349912132 14.459438615464071 6 30.950318091855873 36.68541055347074 18.139440835520986 14.224830519152407 7 27.92111562374584 32.806926627330114 21.332221554057888 17.93973619486616 8 25.88347659980898 34.70893395714215 20.431037692634742 18.976551750414124 9 26.10192092058723 16.70897858283943 20.914353004333652 36.27474749223969 10-11 24.322467583755678 26.80919970069319 28.130077217000903 20.73825549855023 12-13 25.502403283065224 24.230964573602897 28.509786975725564 21.75684516760632 14-15 22.354720134390913 25.477230612032837 27.143673582487615 25.024375671088634 16-17 21.457046399435416 28.85837710757694 27.685158826630186 21.99941766635746 18-19 21.281339206026185 27.302350238864072 30.298060015503598 21.11825053960615 20-21 22.21088452308103 26.334968978020424 30.25666323721711 21.197483261681434 22-23 21.815178046110027 26.388299016851075 30.19889402781079 21.59762890922811 24-25 21.65711925840598 26.432186995005736 30.31991501486331 21.59077873172497 26-27 21.49903075523171 26.69903543046329 30.206714498326008 21.59521931597899 28-29 21.25171174236865 26.93562723046038 30.351080903341053 21.461580123829922 30-31 21.45005085474508 26.573347090887754 30.254892901941922 21.721709152425245 32-33 21.21992234448982 26.70122544690019 30.224341989047872 21.854510219562123 34-35 21.76942221975896 26.72405730432727 29.99912534953544 21.50739512637833 36-37 21.718737519997767 26.431749147328365 30.071943255804083 21.77757007686978 38-39 21.34909482262264 26.892867934754843 30.093673672061627 21.66436357056089 40-41 21.69260576161545 26.740561503501414 30.021132084946522 21.54570064993661 42-43 21.476150069701195 26.73426095088014 29.758058929978443 22.031530049440224 44-45 21.609962289440716 26.918252226732655 29.469932966942814 22.001852516883815 46-47 21.81200600586039 27.167253167450596 29.148576638796335 21.872164187892686 48-49 21.808164374249735 27.157752994827046 29.049091674667665 21.984990956255555 50-51 21.67734594250662 27.372950101871137 28.96422245945126 21.985481496170983 52-53 21.985534428052574 27.499873333139906 28.60291433379511 21.911677905012414 54-55 21.777293814823555 27.22720256663932 28.787687231965226 22.207816386571906 56-57 21.85299852125821 27.488688664249427 28.604173376414245 22.05413943807812 58-59 22.105021878295382 27.12451927489078 28.668055957181913 22.10240288963192 60-61 21.927322972792215 27.177265508021044 29.073970904428403 21.821440614758338 62-63 21.737047760382485 27.33303953162582 29.027190222463883 21.90272248552781 64-65 21.57217198277047 27.360375844632067 28.916821259307213 22.150630913290247 66-67 21.602037094953687 27.42576518216871 28.644256169235582 22.327941553642024 68-69 21.65373281113108 27.26379264969163 28.83788788580808 22.244586653369208 70-71 21.72294542645384 27.19837383486268 28.766031832818932 22.312648905864545 72-73 21.716741225841137 27.133572753852558 28.86312338150892 22.28656263879738 74-75 21.5672943281455 27.5156731380931 28.569690727853107 22.34734180590829 76-77 21.574593103633383 27.198510966986163 28.72250136420377 22.50439456517669 78-79 21.686716737189496 27.264401840945606 28.61283843760748 22.43604298425742 80-81 21.674211592417294 27.450787704101533 28.519970856398498 22.355029847082676 82-83 21.726790272375986 27.27839811987608 28.708079290564037 22.286732317183898 84-85 21.752207666170964 27.22907676148505 28.47815232393374 22.540563248410248 86-87 21.564267840129787 27.205058958124383 28.71404151763549 22.51663168411033 88-89 21.73152244887977 27.048429031837074 28.48134730288148 22.738701216401676 90-91 21.606722725452638 27.01399305217363 28.71872125363895 22.660562968734784 92-93 21.709286795072106 26.84634136258734 28.81198741297397 22.63238442936658 94-95 21.499542678505804 26.782547417075037 28.805886612838506 22.912023291580653 96-97 21.40417066337926 26.83117972567257 28.95194075746505 22.812708853483112 98-99 21.365474955715587 26.916913268354005 28.611733507411902 23.105878268518502 100-101 21.289604746812703 26.659484232551577 29.098359236555442 22.952551784080274 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2821.0 1 1589.5 2 363.5 3 361.0 4 311.0 5 292.5 6 462.0 7 980.0 8 1686.5 9 2098.0 10 2106.0 11 2033.0 12 2150.0 13 2448.5 14 2703.5 15 2890.5 16 3017.5 17 3218.5 18 3546.5 19 3788.0 20 4175.0 21 4882.5 22 5734.5 23 6840.0 24 8446.0 25 10670.5 26 13622.0 27 17349.5 28 20905.0 29 24706.5 30 29433.5 31 34624.5 32 39957.5 33 45261.5 34 51287.5 35 56615.5 36 62053.5 37 67798.5 38 71326.0 39 73220.0 40 75186.0 41 77954.5 42 80439.5 43 83968.5 44 87678.5 45 89513.5 46 88741.5 47 89844.0 48 96357.0 49 95731.5 50 88183.0 51 82052.0 52 76126.5 53 71643.5 54 67363.0 55 61171.0 56 54194.5 57 47716.0 58 42223.5 59 37097.5 60 31412.0 61 25498.0 62 19881.0 63 14994.0 64 11404.5 65 8700.0 66 6348.0 67 4749.5 68 3366.5 69 2171.5 70 1556.5 71 1149.5 72 859.0 73 678.5 74 513.5 75 385.0 76 257.0 77 160.5 78 104.0 79 73.0 80 52.5 81 39.5 82 22.0 83 16.0 84 17.5 85 16.0 86 16.0 87 12.5 88 8.5 89 9.5 90 8.5 91 9.0 92 8.5 93 4.5 94 5.0 95 8.5 96 8.5 97 11.5 98 10.5 99 4.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30028914011174096 2 0.26096504779605745 3 0.25681657212319414 4 0.2624775128851222 5 0.2633849919385611 6 0.1966204615784172 7 0.18547143320759707 8 0.19104594739300715 9 0.1846503807306762 10-11 0.20599774513061866 12-13 0.1967933147314532 14-15 0.19113237396952512 16-17 0.1914564736314676 18-19 0.1865301587699424 20-21 0.18186312363797116 22-23 0.18495287374848915 24-25 0.1751002440254388 26-27 0.17531631046673377 28-29 0.18676783185536686 30-31 0.19808971337922296 32-33 0.19078666766345317 34-35 0.19917004558569779 36-37 0.17965924593676255 38-39 0.18015619875174096 40-41 0.17754179481207188 42-43 0.1833539820829064 44-45 0.1846287740865467 46-47 0.18836672352094957 48-49 0.1942653373683021 50-51 0.1973766941229496 52-53 0.2115722593160287 54-55 0.19307697194117981 56-57 0.17419276497199995 58-59 0.17505703073717982 60-61 0.17250744672989923 62-63 0.17955121271611504 64-65 0.17283154639184167 66-67 0.17071409526715103 68-69 0.167970051462705 70-71 0.17840606057725175 72-73 0.1763534293849496 74-75 0.18060993827846036 76-77 0.1792271130541726 78-79 0.176936808776446 80-81 0.179853705733928 82-83 0.17468971778697837 84-85 0.16915841688982727 86-87 0.16870467736310787 88-89 0.16799165810683447 90-91 0.17406312510722297 92-93 0.17054124211411506 94-95 0.1690503836691798 96-97 0.1672786388505611 98-99 0.17436561812503593 100-101 0.16580938704975534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2314103.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.901127436248444 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95413687273445 43.787771489419676 2 13.09198174289605 14.898970430911321 3 4.379248573904283 7.475525435362104 4 1.8768830060970736 4.271870364514346 5 1.0306404201597876 2.932230094423036 6 0.6122743671098597 2.090346107331989 7 0.39901787977701864 1.5893197058573658 8 0.26522583873426825 1.207331939936358 9 0.20223581198369445 1.0356701138871638 >10 1.0588685821161972 11.120256792508854 >50 0.08640802141075582 3.3715451212925065 >100 0.040798274732220814 4.112532694786253 >500 0.0013683650066675945 0.5029421086936734 >1k 7.602027814815969E-4 0.9131425057640844 >5k 1.5204055629631938E-4 0.6905450953111247 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8987 0.38835782158356824 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6977 0.3014991121829927 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4575 0.19770079378489203 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3115 0.13460939292676255 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3011 0.1301152109478273 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.3213288258992794E-5 2 0.0 0.0 0.0 0.0 4.3213288258992794E-5 3 0.0 0.0 0.0 4.3213288258992794E-5 4.3213288258992794E-5 4 0.0 0.0 0.0 4.3213288258992794E-5 4.3213288258992794E-5 5 0.0 0.0 0.0 4.3213288258992794E-5 4.3213288258992794E-5 6 0.0 0.0 0.0 4.3213288258992794E-5 4.3213288258992794E-5 7 0.0 0.0 0.0 4.3213288258992794E-5 4.3213288258992794E-5 8 0.0 0.0 0.0 8.642657651798559E-5 4.3213288258992794E-5 9 0.0 0.0 0.0 3.4570630607194235E-4 1.2963986477697838E-4 10-11 0.0 0.0 0.0 3.8891959433093516E-4 1.2963986477697838E-4 12-13 0.0 0.0 0.0 5.185594591079135E-4 1.5124650890647478E-4 14-15 0.0 0.0 0.0 6.914126121438847E-4 1.7285315303597118E-4 16-17 0.0 0.0 0.0 8.426591210503596E-4 2.1606644129496398E-4 18-19 0.0 0.0 0.0 9.074790534388487E-4 2.1606644129496398E-4 20-21 0.0 0.0 0.0 9.722989858273379E-4 2.1606644129496398E-4 22-23 0.0 0.0 0.0 0.0011019388506043164 2.1606644129496398E-4 24-25 4.3213288258992794E-5 0.0 0.0 0.0012747920036402874 2.1606644129496398E-4 26-27 4.3213288258992794E-5 0.0 0.0 0.0016637115979712225 2.1606644129496398E-4 28-29 6.481993238848919E-5 0.0 0.0 0.002333517565985611 2.1606644129496398E-4 30-31 8.642657651798559E-5 0.0 0.0 0.004904708217395682 2.1606644129496398E-4 32-33 8.642657651798559E-5 0.0 0.0 0.00911800382264748 2.1606644129496398E-4 34-35 8.642657651798559E-5 0.0 0.0 0.01421717183720863 2.1606644129496398E-4 36-37 8.642657651798559E-5 0.0 0.0 0.0191866999869928 2.3767308542446035E-4 38-39 1.2963986477697838E-4 0.0 0.0 0.023896948407223013 2.8088637368345315E-4 40-41 1.2963986477697838E-4 0.0 0.0 0.028153457300733806 3.0249301781294955E-4 42-43 1.2963986477697838E-4 0.0 0.0 0.03307977216225898 3.0249301781294955E-4 44-45 1.2963986477697838E-4 0.0 0.0 0.0372066411909928 3.0249301781294955E-4 46-47 2.1606644129496395E-4 0.0 0.0 0.04504985300999999 3.0249301781294955E-4 48-49 2.5927972955395675E-4 0.0 0.0 0.05498890930956833 3.0249301781294955E-4 50-51 2.5927972955395675E-4 0.0 0.0 0.06367478024962589 3.0249301781294955E-4 52-53 2.5927972955395675E-4 0.0 0.0 0.08137062179168343 3.0249301781294955E-4 54-55 2.5927972955395675E-4 0.0 0.0 0.1157467926017122 3.0249301781294955E-4 56-57 2.5927972955395675E-4 0.0 0.0 0.17544595033151075 3.4570630607194235E-4 58-59 2.5927972955395675E-4 0.0 0.0 0.24024427607587046 3.8891959433093516E-4 60-61 2.8088637368345315E-4 0.0 0.0 0.29748027637490637 3.8891959433093516E-4 62-63 3.4570630607194235E-4 0.0 0.0 0.3720448052657984 3.8891959433093516E-4 64-65 3.4570630607194235E-4 0.0 0.0 0.4674381390975251 3.8891959433093516E-4 66-67 3.4570630607194235E-4 0.0 0.0 0.6163295237938847 3.8891959433093516E-4 68-69 3.8891959433093516E-4 0.0 0.0 0.7883400177088056 3.8891959433093516E-4 70-71 3.8891959433093516E-4 0.0 0.0 0.9482507909112083 3.8891959433093516E-4 72-73 4.5373952671942436E-4 0.0 0.0 1.1289471557661868 3.8891959433093516E-4 74-75 5.185594591079135E-4 0.0 0.0 1.3303642923413521 3.8891959433093516E-4 76-77 5.185594591079135E-4 0.0 0.0 1.5626789300216974 3.8891959433093516E-4 78-79 5.185594591079135E-4 0.0 0.0 1.7691304146790356 3.8891959433093516E-4 80-81 5.617727473669063E-4 0.0 0.0 1.996929263736316 3.8891959433093516E-4 82-83 6.049860356258991E-4 0.0 0.0 2.268892093394287 3.8891959433093516E-4 84-85 6.049860356258991E-4 0.0 0.0 2.6259202809900857 4.753461708489207E-4 86-87 6.049860356258991E-4 0.0 0.0 3.0254487375886034 4.753461708489207E-4 88-89 6.049860356258991E-4 0.0 0.0 3.5338962872439126 4.753461708489207E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11995 0.0 30.880707 1 GGTATCA 8205 0.0 29.497772 1 GTACATG 9535 0.0 27.527603 1 TACATGG 9480 0.0 26.925018 2 ACATGGG 9345 0.0 26.194973 3 TATCAAC 14525 0.0 25.001568 2 ATCAACG 14420 0.0 24.985842 3 TCAACGC 14495 0.0 24.72807 4 CAACGCA 14505 0.0 24.710485 5 AACGCAG 14760 0.0 24.332172 6 TATACCG 130 2.9274868E-4 21.939947 5 GAGTACT 8290 0.0 21.659336 12-13 ACGCAGA 16455 0.0 21.594334 7 CGCAGAG 16555 0.0 21.406038 8 CATGGGG 4250 0.0 21.140242 4 AGAGTAC 12955 0.0 20.588955 10-11 AGTACTT 8675 0.0 20.342167 12-13 GTACTTT 8800 0.0 20.322672 14-15 CATGGGA 5265 0.0 20.044336 4 GCAGAGT 17260 0.0 19.871153 9 >>END_MODULE