FastQCFastQC Report
Thu 26 May 2016
SRR937410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937410_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314103
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100610.4347688931737265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82130.3549107364711078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54220.23430244894025892No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43060.18607641924322296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA149200.026.2159081
GGTATCA106800.026.2106591
GTACATG102450.024.6793421
TACATGG102550.023.8087222
TATAACG1601.860355E-623.7508222
ACATGGG101350.023.2938483
GAGTACT94800.021.9223812-13
ATCAACG176750.021.8762873
TCAACGC177800.021.720384
CAACGCA178500.021.6884255
TATCAAC179800.021.5844522
AACGCAG183100.021.141726
GTACTTT99450.020.89509614-15
AGTACTT99000.020.68045412-13
AGAGTAC151500.020.30229610-11
CATGGGG44800.020.1457864
TATACCG1456.1471877E-419.6558515
CGTATAG2001.2795343E-519.0105151
ACGCAGA203550.018.9497267
CGCAGAG204050.018.7170528
Produced by FastQC (version 0.11.5)