##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937410_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2314103 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.173519069808044 33.0 31.0 34.0 31.0 34.0 2 32.39227726682866 34.0 31.0 34.0 31.0 34.0 3 32.47990949408907 34.0 31.0 34.0 31.0 34.0 4 36.09309654756076 37.0 35.0 37.0 35.0 37.0 5 35.956294512387736 37.0 35.0 37.0 35.0 37.0 6 36.04219345465608 37.0 35.0 37.0 35.0 37.0 7 36.00550234799402 37.0 35.0 37.0 35.0 37.0 8 35.97952122269406 37.0 35.0 37.0 35.0 37.0 9 37.683514087315906 39.0 37.0 39.0 35.0 39.0 10-11 37.60521268932281 39.0 37.0 39.0 35.0 39.0 12-13 37.52583333585411 39.0 37.0 39.0 35.0 39.0 14-15 38.75019262323241 40.0 38.0 41.0 35.0 41.0 16-17 38.73478406103791 40.0 38.0 41.0 35.0 41.0 18-19 38.74167161098707 40.0 38.0 41.0 35.0 41.0 20-21 38.65591700110151 40.0 38.0 41.0 34.0 41.0 22-23 38.52668636616434 40.0 38.0 41.0 34.0 41.0 24-25 38.38268240437007 40.0 38.0 41.0 34.0 41.0 26-27 38.178602681038825 40.0 38.0 41.0 34.0 41.0 28-29 38.16897324794964 40.0 38.0 41.0 34.0 41.0 30-31 38.29814360034968 40.0 38.0 41.0 34.0 41.0 32-33 38.26420626048193 40.0 38.0 41.0 34.0 41.0 34-35 38.15371852506133 40.0 38.0 41.0 34.0 41.0 36-37 38.00625080214667 40.0 37.5 41.0 33.0 41.0 38-39 37.872143979762356 40.0 37.0 41.0 33.0 41.0 40-41 37.72356092187772 40.0 37.0 41.0 33.0 41.0 42-43 37.52694542982745 40.0 37.0 41.0 33.0 41.0 44-45 37.24791096161234 40.0 37.0 41.0 32.0 41.0 46-47 36.921292613163715 40.0 36.5 41.0 31.0 41.0 48-49 36.612339208756055 40.0 36.0 41.0 30.5 41.0 50-51 36.408079502079204 39.0 36.0 41.0 30.0 41.0 52-53 36.18533725594755 39.0 35.5 41.0 30.0 41.0 54-55 35.988293088077754 39.0 35.0 40.5 29.0 41.0 56-57 35.7524418748863 39.0 35.0 40.0 28.5 41.0 58-59 35.51424569260746 38.5 35.0 40.0 28.0 41.0 60-61 35.19032925500723 38.0 34.5 40.0 28.0 41.0 62-63 34.83746488380163 38.0 34.0 40.0 27.0 41.0 64-65 34.50677065800441 37.0 34.0 40.0 26.0 41.0 66-67 34.13622578597409 37.0 34.0 39.5 26.0 41.0 68-69 33.70597376175563 36.0 33.0 39.0 26.0 40.5 70-71 33.329870148390114 36.0 33.0 39.0 25.5 40.0 72-73 32.896816606693825 35.0 32.5 38.0 24.5 40.0 74-75 32.460047370406585 35.0 32.0 37.0 23.5 39.0 76-77 31.13208098343073 34.0 30.5 36.0 21.5 38.5 78-79 31.749663908650568 35.0 31.5 36.0 23.0 39.0 80-81 31.57527322681834 35.0 32.0 36.0 22.5 37.5 82-83 31.2391514120158 34.5 31.0 35.5 21.0 37.0 84-85 30.908234421717616 34.0 31.0 35.0 20.0 37.0 86-87 30.58285024478167 34.0 31.0 35.0 19.0 36.0 88-89 30.234739551350998 34.0 31.0 35.0 17.5 36.0 90-91 29.805822817739745 34.0 30.0 35.0 12.0 35.5 92-93 29.410344094450423 34.0 30.0 35.0 4.5 35.0 94-95 29.024795136603686 34.0 29.0 35.0 2.0 35.0 96-97 28.622851489324376 34.0 29.0 35.0 2.0 35.0 98-99 28.009190818213362 33.5 29.0 35.0 2.0 35.0 100-101 26.408748443781455 32.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0512998116451584 1101 2 0.09516885476942605 1101 3 0.06262080826041228 1101 4 0.09540854546358446 1101 5 -0.014369299159312732 1101 6 0.025452986475144712 1101 7 -8.16646132129506E-4 1101 8 -0.007942389256598403 1101 9 0.07222565124611435 1101 10-11 0.1070411800067319 1101 12-13 0.11700460966176962 1101 14-15 0.1141797654931409 1101 16-17 0.09317189414270644 1101 18-19 0.159792783835762 1101 20-21 0.09185544857258066 1101 22-23 -0.006731552890819614 1101 24-25 0.06601721516481263 1101 26-27 0.016573065404621445 1101 28-29 0.08330128156207905 1101 30-31 0.14720882572398608 1101 32-33 0.14006333003366223 1101 34-35 0.18841205913582115 1101 36-37 0.2216634406507083 1101 38-39 0.24990557712951755 1101 40-41 0.16234693767774644 1101 42-43 0.20495884079105764 1101 44-45 0.21120008202753127 1101 46-47 0.2811409382265495 1101 48-49 0.20086812095515683 1101 50-51 0.24216671481926255 1101 52-53 0.35514140457156174 1101 54-55 0.20903828706683925 1101 56-57 0.1972129779748144 1101 58-59 0.20165818302172767 1101 60-61 0.26324878515455197 1101 62-63 0.2388641928801647 1101 64-65 0.25040338254509464 1101 66-67 0.20040078847993925 1101 68-69 0.09017797410425032 1101 70-71 -0.06932823387850817 1101 72-73 0.16494101501803726 1101 74-75 0.18020773970240356 1101 76-77 0.002132415522165587 1101 78-79 0.020167231773438488 1101 80-81 0.05229630661845519 1101 82-83 0.05201196454925494 1101 84-85 0.03262390559301309 1101 86-87 -0.07481002673948822 1101 88-89 -0.050886007814895606 1101 90-91 0.11694230219205792 1101 92-93 0.20852880350570402 1101 94-95 0.07543434586419906 1101 96-97 -0.02805089806432548 1101 98-99 -0.09735654049326925 1101 100-101 0.1252079819093197 1102 1 0.12777096114689357 1102 2 0.18905946955261754 1102 3 0.04808358647316879 1102 4 0.05340785510585988 1102 5 -0.025098608773525655 1102 6 0.06638235951245974 1102 7 0.05697878347523755 1102 8 0.1272979871286779 1102 9 0.07380940482769205 1102 10-11 0.16447557497554044 1102 12-13 0.15195004053199312 1102 14-15 0.22925587322261975 1102 16-17 0.14712166640885727 1102 18-19 0.20902490118535155 1102 20-21 0.2012892850812804 1102 22-23 0.1474817339696486 1102 24-25 0.18426847731272034 1102 26-27 0.07001783818993346 1102 28-29 0.10017877393586616 1102 30-31 0.0739603979716108 1102 32-33 0.09413693170848347 1102 34-35 0.2352742990940655 1102 36-37 0.2558431637779677 1102 38-39 0.20540675999854585 1102 40-41 0.28112852645466546 1102 42-43 0.25958641244373837 1102 44-45 0.1838593203328429 1102 46-47 0.10021508713614935 1102 48-49 0.23768944458980457 1102 50-51 0.17354971742634717 1102 52-53 0.10482113190833076 1102 54-55 0.14581948015091228 1102 56-57 0.16806837609513536 1102 58-59 0.06752609738369841 1102 60-61 0.11217641545957235 1102 62-63 0.13876239317931294 1102 64-65 0.1486546166296847 1102 66-67 0.09871376187310688 1102 68-69 0.03800895994069009 1102 70-71 0.03110415175643766 1102 72-73 0.013228520946412914 1102 74-75 -0.07800744704497475 1102 76-77 -0.10423627479065445 1102 78-79 0.1383325499974788 1102 80-81 0.11340369933938987 1102 82-83 0.1313532903167136 1102 84-85 0.11100476416228844 1102 86-87 0.10219130907464091 1102 88-89 0.18374181640767873 1102 90-91 0.10872872908569775 1102 92-93 0.040774893583353844 1102 94-95 0.03306693804519156 1102 96-97 0.19423320378308162 1102 98-99 0.2863652593759589 1102 100-101 0.35387157844854755 1103 1 0.06526784539199326 1103 2 0.01980101256172162 1103 3 0.06463210963123345 1103 4 0.02888773313542714 1103 5 -0.04632923321592841 1103 6 -0.027042358449776316 1103 7 -0.03032729452862526 1103 8 0.013862319460677952 1103 9 -0.006809098222383625 1103 10-11 0.03827385512320802 1103 12-13 -0.0068368172767137025 1103 14-15 -0.06685104927840513 1103 16-17 -0.061523482799010765 1103 18-19 0.037372804787111136 1103 20-21 -0.03988759970820155 1103 22-23 0.018515664595973647 1103 24-25 -0.10233866567649841 1103 26-27 0.003825742599381954 1103 28-29 -0.0010112558259791626 1103 30-31 0.03575698995334875 1103 32-33 0.1288343480838421 1103 34-35 0.14917672718613773 1103 36-37 0.10192620313936374 1103 38-39 0.10328847566152177 1103 40-41 0.1318262854336254 1103 42-43 0.09760894765416595 1103 44-45 -0.028604320564141972 1103 46-47 -0.22145670601056366 1103 48-49 -0.2249323694075045 1103 50-51 -0.2205961249029187 1103 52-53 -0.13063814848505473 1103 54-55 -0.25597296094264976 1103 56-57 -0.16558433598366662 1103 58-59 -0.16116602224531817 1103 60-61 -0.2837129182518723 1103 62-63 -0.27141074442025825 1103 64-65 -0.24984559024904485 1103 66-67 -0.3168465806654126 1103 68-69 -0.34850352327121215 1103 70-71 -0.30683332426981735 1103 72-73 -0.3408240462211225 1103 74-75 -0.3753368549339413 1103 76-77 -0.5842199996403181 1103 78-79 -0.3013522043937549 1103 80-81 -0.37047950833563803 1103 82-83 -0.4374133618677014 1103 84-85 -0.45016284843647014 1103 86-87 -0.3368777647521455 1103 88-89 -0.264195380032735 1103 90-91 -0.3009211096198996 1103 92-93 -0.21762773339928287 1103 94-95 -0.21889818798515037 1103 96-97 -0.09928141064215623 1103 98-99 -0.29131737045875283 1103 100-101 -0.1642079360602544 1104 1 0.020158299272004854 1104 2 0.12403713410002837 1104 3 0.07033566939624336 1104 4 0.006102087389876942 1104 5 -0.09100885319171681 1104 6 -0.020574697341842807 1104 7 0.012370442171231844 1104 8 0.04342678022889146 1104 9 0.08259985010671045 1104 10-11 0.12607241331632224 1104 12-13 0.15724767758059244 1104 14-15 0.06193184623018766 1104 16-17 0.1458223303186088 1104 18-19 0.19813453655073943 1104 20-21 0.04643612998614799 1104 22-23 -0.009396175910218574 1104 24-25 0.04050231619739719 1104 26-27 0.09503020444682164 1104 28-29 0.03183586407943295 1104 30-31 0.024357715255767687 1104 32-33 0.03628490071196211 1104 34-35 0.10725496905214271 1104 36-37 0.1438374770580424 1104 38-39 0.030449573034807997 1104 40-41 0.05167731866404068 1104 42-43 0.10296044306986118 1104 44-45 0.23291401962767821 1104 46-47 0.17787796421907132 1104 48-49 0.014584295069454356 1104 50-51 0.07053066577172729 1104 52-53 0.17790600311170834 1104 54-55 0.09540918122741715 1104 56-57 0.06055278347596982 1104 58-59 0.07896451869913079 1104 60-61 0.0556933173781573 1104 62-63 0.14827452090224114 1104 64-65 0.12448290675730789 1104 66-67 0.007717305303870603 1104 68-69 0.05945650356837717 1104 70-71 -0.05580733749253852 1104 72-73 0.07481394540792508 1104 74-75 -0.04886110265116628 1104 76-77 0.018188862468935696 1104 78-79 0.02219984482310977 1104 80-81 0.12199487791986385 1104 82-83 0.1496772551848231 1104 84-85 0.17949111673775064 1104 86-87 0.1838856738124086 1104 88-89 0.2581209687740298 1104 90-91 0.17525662700957412 1104 92-93 0.20370054776420332 1104 94-95 0.20164912911148392 1104 96-97 0.3348435518199544 1104 98-99 0.4443558393358238 1104 100-101 0.5732387923953439 1105 1 0.08668638949222185 1105 2 0.08547287291129635 1105 3 0.08424861635338488 1105 4 0.022549074129166513 1105 5 -0.07959146626298974 1105 6 0.009861603865829238 1105 7 -0.013238869929168118 1105 8 -0.04499362588050815 1105 9 0.03658324461299145 1105 10-11 0.12992120268407348 1105 12-13 0.035277588189167375 1105 14-15 0.12398807107035026 1105 16-17 0.1329493727055322 1105 18-19 0.1650989279786259 1105 20-21 0.11366077774840022 1105 22-23 0.10966558409450755 1105 24-25 0.12384302481685694 1105 26-27 0.24247130082380153 1105 28-29 0.06584575041615892 1105 30-31 -0.03294389201394665 1105 32-33 0.12494143931011337 1105 34-35 0.1937291876171443 1105 36-37 0.1525839990802993 1105 38-39 0.08141822683542443 1105 40-41 0.12113830611372123 1105 42-43 -0.006329161476671175 1105 44-45 0.16094319024098525 1105 46-47 0.3169821259524426 1105 48-49 0.18580469525888788 1105 50-51 0.10247959129860362 1105 52-53 0.10134046913112371 1105 54-55 0.21228114724683422 1105 56-57 0.18452500405376782 1105 58-59 0.16041128343984212 1105 60-61 0.11240440842717447 1105 62-63 0.19875470382906713 1105 64-65 0.376760834178576 1105 66-67 0.10588374503865339 1105 68-69 0.06670904915208098 1105 70-71 0.057164927484961936 1105 72-73 0.1672580017659655 1105 74-75 0.2270920852484366 1105 76-77 0.14519669161883186 1105 78-79 0.09905923092303581 1105 80-81 0.0520592575078318 1105 82-83 0.048277998140068945 1105 84-85 0.11035617120140273 1105 86-87 0.10070517416760794 1105 88-89 0.33541014412330483 1105 90-91 0.12995626293190554 1105 92-93 0.2199401138309831 1105 94-95 0.2044666610792767 1105 96-97 0.3629795037498589 1105 98-99 0.362418457735064 1105 100-101 0.4469385467169147 1106 1 -0.1356283587749445 1106 2 -0.0362799785990191 1106 3 -0.08240188938617621 1106 4 -0.04756190027728735 1106 5 -0.07642912203524332 1106 6 -0.11644424370982875 1106 7 -0.09333161253594824 1106 8 -0.0960506236025509 1106 9 -0.18900380715476217 1106 10-11 -0.21792439955409293 1106 12-13 -0.3686018464220169 1106 14-15 -0.16413543414540044 1106 16-17 -0.0769343741932289 1106 18-19 -0.10444178497000678 1106 20-21 -0.29325942868916854 1106 22-23 -0.22963892756531123 1106 24-25 -0.20705577448310208 1106 26-27 -0.041647542898409995 1106 28-29 -0.3056151080620353 1106 30-31 -0.24850678360173362 1106 32-33 -0.1723827496996435 1106 34-35 -0.2141597811289202 1106 36-37 -0.20156753194421384 1106 38-39 -0.17793460518432624 1106 40-41 -0.1625498773249845 1106 42-43 -0.3639352327877816 1106 44-45 -0.4528053733449582 1106 46-47 -0.29904212355928195 1106 48-49 -0.509144487152497 1106 50-51 -0.23399091094889712 1106 52-53 -0.21435926246754633 1106 54-55 -0.15495350697835164 1106 56-57 -0.31275841719013897 1106 58-59 -0.33478924605385174 1106 60-61 -0.2452973290755125 1106 62-63 -0.26638122256156294 1106 64-65 -0.3142119480590253 1106 66-67 -0.1126370022621046 1106 68-69 -0.12021944077576308 1106 70-71 -0.048547676007913765 1106 72-73 -0.14493765102585598 1106 74-75 -0.045681544016652254 1106 76-77 -0.08820055452670772 1106 78-79 -0.20598825702732526 1106 80-81 -0.015123259455048554 1106 82-83 -0.3615683106310925 1106 84-85 -0.2837403801866891 1106 86-87 0.042277088262938634 1106 88-89 -0.1003741278025636 1106 90-91 0.029727167578371905 1106 92-93 -0.02668010032160595 1106 94-95 -0.11499791684846983 1106 96-97 -0.037749524604851814 1106 98-99 -0.19385275348175668 1106 100-101 -0.19937355408179158 1107 1 -0.023145868786798474 1107 2 -0.05435306803829576 1107 3 0.006462674073830499 1107 4 -0.018909397742092438 1107 5 -0.0921521458746426 1107 6 -0.025628155410672093 1107 7 -0.04399545325141929 1107 8 -0.03496968652687116 1107 9 -0.11064793639512516 1107 10-11 -0.050858655009776044 1107 12-13 0.01580566705603559 1107 14-15 -0.07847128190211805 1107 16-17 -0.1112723489329781 1107 18-19 -0.02466658272757627 1107 20-21 -0.02954584879768163 1107 22-23 0.030626898764019472 1107 24-25 0.0868004493413963 1107 26-27 0.03570427728343617 1107 28-29 -0.12705049576019434 1107 30-31 -0.015085882305207576 1107 32-33 -0.13278407272606074 1107 34-35 -0.11166730792746904 1107 36-37 -0.21879251375385422 1107 38-39 -0.18199404540822428 1107 40-41 -0.12120002012647291 1107 42-43 -0.10001316200866484 1107 44-45 -0.12845782718154908 1107 46-47 -0.1246612940502203 1107 48-49 -0.17837114261848086 1107 50-51 -0.22318331267798186 1107 52-53 -0.2548850927787001 1107 54-55 -0.38414989411504763 1107 56-57 -0.19886241448593012 1107 58-59 -0.2252322529346742 1107 60-61 -0.19847004296263293 1107 62-63 -0.10537200849969963 1107 64-65 -0.0904378458320636 1107 66-67 -0.12676836960424964 1107 68-69 -0.279130407557588 1107 70-71 -0.4009094507568278 1107 72-73 -0.12427037634282101 1107 74-75 -0.1320891556080639 1107 76-77 -0.17467521621465565 1107 78-79 -0.20986507749563543 1107 80-81 -0.33357854339654125 1107 82-83 -0.31931027822647096 1107 84-85 -0.29123745827060077 1107 86-87 -0.2699701982223388 1107 88-89 -0.06540736817886028 1107 90-91 -0.13756443463426393 1107 92-93 -0.04600449316462729 1107 94-95 -0.1636798677144462 1107 96-97 0.04284451239462683 1107 98-99 0.0016379350591755326 1107 100-101 0.185743490858421 1108 1 -0.05454822559836714 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76-77 -0.09475398223392872 2316 78-79 -0.08562312245999948 2316 80-81 -0.15838211135534763 2316 82-83 -0.23252661898762383 2316 84-85 -0.22566718986223577 2316 86-87 -0.18296311375186747 2316 88-89 -0.2834445878692051 2316 90-91 -0.3222600960723092 2316 92-93 -0.3795503495718968 2316 94-95 -0.532771450255094 2316 96-97 -0.45299665945207934 2316 98-99 -0.5863016288986778 2316 100-101 -0.8105678537888679 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 4.0 4 18.0 5 35.0 6 81.0 7 270.0 8 503.0 9 1009.0 10 1761.0 11 2911.0 12 4424.0 13 6322.0 14 8238.0 15 11355.0 16 15364.0 17 21878.0 18 19518.0 19 11426.0 20 9634.0 21 9610.0 22 9754.0 23 10965.0 24 12547.0 25 15120.0 26 18347.0 27 23274.0 28 29490.0 29 37537.0 30 47360.0 31 60616.0 32 78411.0 33 103296.0 34 142897.0 35 206617.0 36 327060.0 37 528906.0 38 485868.0 39 51667.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.47031733280991 20.067610159423012 15.27912645495005 22.18294605281703 2 15.466597640640888 21.314911220459937 39.655192530323845 23.56329860857533 3 19.256921580413664 25.09551217037444 30.240831976796194 25.406734272415704 4 12.816121681810777 17.60722630516127 37.63007751174214 31.946574501285813 5 13.294249589966443 38.10721162053474 34.38036852238815 14.218170267110663 6 31.229292732432395 37.32232316366212 17.39066930037254 14.057714803532944 7 28.136388051871503 33.333607017492305 20.769300242901892 17.760704687734297 8 26.32609698012578 35.34885871545044 19.65828660176319 18.6667577026606 9 26.163658229560223 16.905340859935794 20.542344052965664 36.38865685753832 10-11 24.55618872625808 27.428532783545073 27.43672170167015 20.5785567885267 12-13 25.745095950462847 24.706776915362944 28.046779132321987 21.501348001852218 14-15 22.40928774561893 26.07960838389648 26.65170910715729 24.859394763327302 16-17 21.5833521671248 29.513401088888436 27.161042529221906 21.742204214764858 18-19 21.453085709668066 27.78700861629755 29.896616529169183 20.863289144865202 20-21 22.30207557744837 26.735650919600378 30.033883539323874 20.928389963627374 22-23 21.937433234575245 26.91863088097518 29.839996335464065 21.30393954898551 24-25 21.901830177884023 26.961352444068776 30.003776859346992 21.13304051870021 26-27 21.63929623077076 27.246015002242817 29.943834499972777 21.17085426701365 28-29 21.42709287652763 27.484853330106652 29.98621458574614 21.101839207619573 30-31 21.711663378457885 27.069178568642826 29.862582330359434 21.356575722539855 32-33 21.53130176141684 27.201209280658638 29.815786073480737 21.451702884443776 34-35 21.951788662820974 27.18275720657205 29.751571127127875 21.113883003479103 36-37 21.94716640969087 26.96374739855218 29.702635499996543 21.386450691760412 38-39 21.629813494963255 27.389175802355393 29.766651556354905 21.214359146326444 40-41 21.90641471014903 27.22387465035048 29.64323541346258 21.22647522603791 42-43 21.724136589784635 27.392464846437626 29.31607867068191 21.567319893095828 44-45 21.84935156300303 27.433480705050727 29.133318612006466 21.583849119939778 46-47 22.083589192010898 27.788693934539648 28.673118698692324 21.45459817475713 48-49 22.137308092446204 27.79433927408142 28.535857854178886 21.532494779293483 50-51 21.990183669439087 28.003701650272266 28.407443402476034 21.598671277812613 52-53 22.38742923814874 28.227518257637957 27.90806361004278 21.47698889417052 54-55 22.06077257581015 27.96964957912418 28.139326555473115 21.830251289592557 56-57 22.140911618886456 28.229858394375707 27.952774790059042 21.6764551966788 58-59 22.408488299786136 27.87306787986533 27.973041822252508 21.74540199809602 60-61 22.187409117053132 27.932529364509705 28.424555864626594 21.45550565381057 62-63 22.03936039147782 28.039979205765693 28.385123739090268 21.535536663666225 64-65 21.90297925373244 28.070250114191115 28.264925977797876 21.76184465427857 66-67 21.946214148635562 28.140687774053276 28.017076162988424 21.89602191432274 68-69 22.00988892888519 27.914401390085057 28.15285231469818 21.922857366331577 70-71 22.017408041042252 28.021505525034968 28.109034040403557 21.852052393519216 72-73 22.082854566110495 27.825273983050884 28.154580846228537 21.93729060461008 74-75 21.917045179060743 28.301786912682797 27.805849609978466 21.975318298277994 76-77 21.891268452614252 27.938557618221832 28.076624074209317 22.093549854954595 78-79 21.99260361358159 28.00361522369575 27.907552083896007 22.096229078826656 80-81 21.962008605494223 28.260993568566306 27.856992536632987 21.92000528930648 82-83 21.96986982367166 28.13978441652277 27.894547839930304 21.99579791987527 84-85 21.924155843706874 28.100767447221937 27.75484470099721 22.220232008073978 86-87 21.805490075420153 28.009557050831358 27.92462133275831 22.26033154099018 88-89 21.879082305325216 28.01727062278559 27.615581501774123 22.488065570115072 90-91 21.80112626302678 27.947213740400283 27.771193276562805 22.48046672001013 92-93 21.899680105961526 27.836917781506216 27.860231909673416 22.403170202858842 94-95 21.67464023857192 27.7709332730652 27.880457352157617 22.67396913620526 96-97 21.623821411579346 27.793404182959875 27.955151520913287 22.627622884547492 98-99 21.564474675004078 28.026667329280585 27.482193151236945 22.926664844478392 100-101 21.2467200080213 28.190330401049856 27.621665201929517 22.94128438899932 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 306.0 1 226.0 2 181.5 3 228.5 4 213.5 5 228.5 6 430.0 7 899.5 8 1560.0 9 2060.5 10 2148.5 11 2166.5 12 2380.0 13 2690.5 14 3031.5 15 3256.5 16 3388.5 17 3514.0 18 3736.5 19 4091.0 20 4555.5 21 5188.0 22 5993.0 23 7167.0 24 8702.0 25 10829.5 26 13819.5 27 17371.5 28 20879.5 29 24886.5 30 29841.5 31 34932.5 32 40039.5 33 45158.0 34 51195.5 35 56474.5 36 61804.0 37 67420.5 38 70875.5 39 73486.5 40 75415.0 41 77649.0 42 80010.0 43 83727.0 44 88295.5 45 89958.0 46 88451.0 47 90110.0 48 97257.0 49 96251.0 50 88785.0 51 82663.0 52 76426.0 53 71606.0 54 66913.5 55 60818.0 56 53546.0 57 46972.0 58 42037.0 59 37296.5 60 31322.0 61 24908.0 62 19620.0 63 15078.5 64 11193.5 65 8571.0 66 6271.0 67 4712.5 68 3367.5 69 2087.0 70 1450.5 71 1057.5 72 813.5 73 660.0 74 521.0 75 401.5 76 272.0 77 147.0 78 89.5 79 61.5 80 41.5 81 22.0 82 10.5 83 9.0 84 7.5 85 4.0 86 2.0 87 3.0 88 3.5 89 1.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.049133508750474814 2 0.0 3 0.0 4 8.642657651798559E-5 5 0.011581161253410069 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005293627811726617 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013396119360287765 24-25 4.753461708489207E-4 26-27 0.002117451124690647 28-29 0.002722437160316546 30-31 0.001145152138863309 32-33 0.0 34-35 0.0 36-37 3.0249301781294955E-4 38-39 4.321328825899279E-4 40-41 0.0 42-43 0.011019388506043162 44-45 0.0 46-47 0.0 48-49 2.1606644129496397E-5 50-51 0.0 52-53 1.2963986477697838E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.753461708489207E-4 84-85 1.0803322064748198E-4 86-87 0.0 88-89 0.0 90-91 0.007238225783381293 92-93 0.0023983374983740998 94-95 0.0 96-97 0.0 98-99 0.0015340717331942442 100-101 0.011386701456244602 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2314103.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.03786579837928 #Duplication Level Percentage of deduplicated Percentage of total 1 75.55686726504184 41.5848872067933 2 13.750261249808313 15.135700667192095 3 4.674748352155641 7.718645173414104 4 2.0822585068002946 4.584122570192329 5 1.08329538766421 2.9811133083133026 6 0.6470857617428597 2.136853158890732 7 0.405241560731784 1.5612541444837187 8 0.28879869135860553 1.271589089419399 9 0.21905406366221347 1.0850641342587484 >10 1.151198680975035 11.602504672202665 >50 0.09503773405193638 3.613373533536135 >100 0.04324645945624142 4.3361468721562515 >500 0.0020422554143099034 0.7507547519179495 >1k 6.283862813257881E-4 0.613581837727001 >5k 1.5709657033144702E-4 0.589458772451748 >10k+ 7.854828516572351E-5 0.4349501070507545 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10061 0.4347688931737265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8213 0.3549107364711078 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5422 0.23430244894025892 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4306 0.18607641924322296 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2963986477697838E-4 2 0.0 0.0 0.0 0.0 1.2963986477697838E-4 3 0.0 0.0 0.0 0.0 1.2963986477697838E-4 4 0.0 0.0 0.0 0.0 1.2963986477697838E-4 5 0.0 0.0 0.0 0.0 1.2963986477697838E-4 6 4.3213288258992794E-5 0.0 0.0 0.0 1.2963986477697838E-4 7 4.3213288258992794E-5 0.0 0.0 0.0 1.2963986477697838E-4 8 4.3213288258992794E-5 0.0 0.0 0.0 1.2963986477697838E-4 9 4.3213288258992794E-5 0.0 0.0 0.0 2.5927972955395675E-4 10-11 1.7285315303597118E-4 0.0 0.0 2.1606644129496397E-5 2.5927972955395675E-4 12-13 1.9445979716546758E-4 0.0 0.0 4.3213288258992794E-5 2.5927972955395675E-4 14-15 2.5927972955395675E-4 0.0 0.0 1.0803322064748199E-4 2.5927972955395675E-4 16-17 2.5927972955395675E-4 0.0 0.0 2.3767308542446035E-4 2.5927972955395675E-4 18-19 2.8088637368345315E-4 0.0 0.0 2.5927972955395675E-4 2.5927972955395675E-4 20-21 3.0249301781294955E-4 0.0 0.0 2.8088637368345315E-4 2.5927972955395675E-4 22-23 3.0249301781294955E-4 0.0 0.0 4.321328825899279E-4 2.5927972955395675E-4 24-25 3.4570630607194235E-4 0.0 0.0 7.130192562733811E-4 2.5927972955395675E-4 26-27 3.4570630607194235E-4 0.0 0.0 0.0012099720712517982 2.5927972955395675E-4 28-29 3.6731295020143875E-4 0.0 0.0 0.002009417904043165 2.5927972955395675E-4 30-31 3.8891959433093516E-4 0.0 0.0 0.004667035131971222 2.5927972955395675E-4 32-33 4.1052623846043156E-4 0.0 0.0 0.00892354402548201 3.4570630607194235E-4 34-35 4.3213288258992796E-4 0.0 0.0 0.013979498751784168 3.4570630607194235E-4 36-37 4.3213288258992796E-4 0.0 0.0 0.018970633545697835 4.1052623846043156E-4 38-39 5.185594591079135E-4 0.0 0.0 0.02374570189831654 4.753461708489207E-4 40-41 5.185594591079135E-4 0.0 0.0 0.02800221079182733 4.753461708489207E-4 42-43 5.401661032374099E-4 0.0 0.0 0.03282049243270503 4.753461708489207E-4 44-45 5.617727473669063E-4 0.0 0.0 0.03701218139382732 4.753461708489207E-4 46-47 6.049860356258991E-4 0.0 0.0 0.04494181978935251 4.753461708489207E-4 48-49 6.481993238848919E-4 0.0 0.0 0.05496730266543883 4.753461708489207E-4 50-51 6.481993238848919E-4 0.0 0.0 0.06365317360549638 4.753461708489207E-4 52-53 6.481993238848919E-4 0.0 0.0 0.08139222843581292 4.753461708489207E-4 54-55 6.481993238848919E-4 0.0 0.0 0.11622213877256111 4.753461708489207E-4 56-57 6.481993238848919E-4 0.0 0.0 0.17648306924972657 4.753461708489207E-4 58-59 6.481993238848919E-4 0.0 0.0 0.24210244747100712 4.753461708489207E-4 60-61 6.481993238848919E-4 0.0 0.0 0.299813793940892 4.753461708489207E-4 62-63 6.481993238848919E-4 0.0 0.0 0.37504812879979843 4.753461708489207E-4 64-65 6.481993238848919E-4 0.0 0.0 0.4712625151084459 4.753461708489207E-4 66-67 6.481993238848919E-4 0.0 0.0 0.6210829855023738 4.753461708489207E-4 68-69 6.914126121438847E-4 0.0 0.0 0.7940657784031221 4.753461708489207E-4 70-71 6.914126121438847E-4 0.0 0.0 0.9552945568974243 4.753461708489207E-4 72-73 6.914126121438847E-4 0.0 0.0 1.1369848273823595 4.753461708489207E-4 74-75 6.914126121438847E-4 0.0 0.0 1.339979248978978 4.753461708489207E-4 76-77 6.914126121438847E-4 0.0 0.0 1.5742168779868484 5.185594591079135E-4 78-79 6.914126121438847E-4 0.0 0.0 1.783606866245798 5.185594591079135E-4 80-81 7.778391886618703E-4 0.0 0.0 2.0133719199188627 5.185594591079135E-4 82-83 7.778391886618703E-4 0.0 0.0 2.2883596797549632 5.185594591079135E-4 84-85 7.778391886618703E-4 0.0 0.0 2.6495795563118842 5.185594591079135E-4 86-87 7.778391886618703E-4 0.0 0.0 3.056194992184877 5.185594591079135E-4 88-89 7.778391886618703E-4 0.0 0.0 3.572356113794416 5.185594591079135E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14920 0.0 26.215908 1 GGTATCA 10680 0.0 26.210659 1 GTACATG 10245 0.0 24.679342 1 TACATGG 10255 0.0 23.808722 2 TATAACG 160 1.860355E-6 23.750822 2 ACATGGG 10135 0.0 23.293848 3 GAGTACT 9480 0.0 21.92238 12-13 ATCAACG 17675 0.0 21.876287 3 TCAACGC 17780 0.0 21.72038 4 CAACGCA 17850 0.0 21.688425 5 TATCAAC 17980 0.0 21.584452 2 AACGCAG 18310 0.0 21.14172 6 GTACTTT 9945 0.0 20.895096 14-15 AGTACTT 9900 0.0 20.680454 12-13 AGAGTAC 15150 0.0 20.302296 10-11 CATGGGG 4480 0.0 20.145786 4 TATACCG 145 6.1471877E-4 19.655851 5 CGTATAG 200 1.2795343E-5 19.010515 1 ACGCAGA 20355 0.0 18.949726 7 CGCAGAG 20405 0.0 18.717052 8 >>END_MODULE