FastQCFastQC Report
Thu 26 May 2016
SRR937409_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937409_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518954
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94410.3747984282364823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75260.2987748089087772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48960.19436639176420054No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34390.13652492264646357No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33900.13457967076810454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA139750.029.8349421
GGTATCA95550.029.8039321
GTACATG108600.026.8354021
TACATGG107800.026.1431392
ACATGGG106750.025.8660453
ATCAACG163500.025.3757133
TCAACGC164500.025.2234614
CAACGCA164500.025.1920625
TATCAAC166800.024.9876462
AACGCAG168400.024.6255826
GAGTACT90550.023.50031312-13
TAGCGCG1050.002189230222.6327384
ACGCAGA187100.022.0124457
CGCAGAG187650.021.8972998
GTACTTT97150.021.4884214-15
CATGGGA60050.021.290984
AGTACTT96000.020.9539712-13
GCAGAGT195500.020.6041569
AGAGTAC147600.020.5641910-11
CATGGGG47000.019.1126234
Produced by FastQC (version 0.11.5)