FastQCFastQC Report
Thu 26 May 2016
SRR937409_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937409_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518954
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106150.4214050752812477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88340.3507011243555857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58790.23339052638515828No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45120.17912196888073384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA157200.027.0337121
GGTATCA112300.025.5245591
GTACATG110300.022.9706021
TATAACG1452.3336499E-522.9337332
ATCAACG186500.022.5173933
TCAACGC187350.022.440594
CAACGCA188300.022.4282885
TACATGG109450.022.3530182
TATCAAC188550.022.297772
ACATGGG106950.022.0759943
AACGCAG192800.021.8758246
GAGTACT99500.021.72107312-13
ATACCGT1805.176411E-621.1094046
GTACTTT104350.020.48229214-15
CGTATAG1404.829355E-420.372481
AGTACTT104150.020.34082412-13
AGAGTAC162400.020.31324210-11
ACGCAGA213650.019.6091467
CGCAGAG215050.019.4814878
TGCACCG2502.1719097E-719.0022355
Produced by FastQC (version 0.11.5)