FastQCFastQC Report
Thu 26 May 2016
SRR937408_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937408_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665422
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98320.36887217108585435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77730.2916236153224518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48630.18244765744411204No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37680.14136598257236566No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35030.13142384207829005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97600.030.0855461
GTATCAA141050.029.6434541
GTACATG114700.024.688921
ATCAACG167850.024.6806833
TCAACGC168100.024.6453694
CAACGCA168300.024.5878525
TATCAAC169550.024.48882
TACATGG112500.024.1544632
AACGCAG171800.024.1118686
ACATGGG112050.023.2343623
GAGTACT91750.022.46882612-13
ACGCAGA192100.021.4154937
CGCAGAG193800.021.2031258
GTGTAGC14500.020.6439021
GTACTTT98150.020.51860214-15
AGTACTT97150.020.19314412-13
AGAGTAC154700.020.03545410-11
GCAGAGT205150.019.821659
GTACCTG21100.019.1406081
GTTCGCG1759.9353034E-519.001927
Produced by FastQC (version 0.11.5)