##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937408_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2665422 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.691259020147655 33.0 31.0 34.0 30.0 34.0 2 31.78483519682812 33.0 31.0 34.0 30.0 34.0 3 31.829639359170894 33.0 31.0 34.0 30.0 34.0 4 35.44137476167001 37.0 35.0 37.0 33.0 37.0 5 35.46088836964653 37.0 35.0 37.0 33.0 37.0 6 35.48190830570169 37.0 35.0 37.0 33.0 37.0 7 35.36785019407809 37.0 35.0 37.0 33.0 37.0 8 35.37571086304533 37.0 35.0 37.0 33.0 37.0 9 36.994165276642875 39.0 37.0 39.0 33.0 39.0 10-11 36.879045607037085 39.0 37.0 39.0 33.0 39.0 12-13 36.734846864774134 39.0 37.0 39.0 32.5 39.0 14-15 37.901809732192504 40.0 38.0 41.0 33.0 41.0 16-17 37.83152799068965 40.0 38.0 41.0 33.0 41.0 18-19 37.79628741715196 40.0 38.0 41.0 33.0 41.0 20-21 37.686387183718 40.0 37.5 41.0 32.0 41.0 22-23 37.52806572467699 40.0 37.0 41.0 32.0 41.0 24-25 37.464400946641845 40.0 37.0 41.0 32.0 41.0 26-27 37.50033747001413 40.0 37.0 41.0 32.0 41.0 28-29 37.42015391934186 40.0 37.0 41.0 32.0 41.0 30-31 37.22074253157662 39.5 37.0 41.0 31.5 41.0 32-33 37.00048134966996 39.0 36.0 41.0 31.0 41.0 34-35 37.024897183260286 39.5 36.0 41.0 31.0 41.0 36-37 36.9005153780527 39.5 36.0 41.0 30.5 41.0 38-39 36.79642416848064 39.0 36.0 41.0 30.0 41.0 40-41 36.58879944714195 39.0 36.0 41.0 30.0 41.0 42-43 36.33192417560896 39.0 35.0 41.0 30.0 41.0 44-45 36.107416386598445 39.0 35.0 41.0 29.5 41.0 46-47 35.78228194259671 39.0 35.0 41.0 27.5 41.0 48-49 35.47316016000468 39.0 35.0 40.0 27.0 41.0 50-51 34.50496619297057 38.0 34.0 39.5 25.0 40.5 52-53 34.63553257232813 38.0 34.0 39.5 26.0 40.5 54-55 35.01897466892672 39.0 35.0 40.0 26.0 41.0 56-57 34.808206167728784 38.0 34.0 40.0 25.5 41.0 58-59 34.54588204044238 38.0 34.0 40.0 24.0 41.0 60-61 34.24462711720695 38.0 34.0 40.0 23.0 41.0 62-63 33.93889898109943 37.5 33.5 40.0 22.5 41.0 64-65 33.52656802562596 37.0 33.0 40.0 22.0 41.0 66-67 33.17514487386988 36.5 32.5 39.0 20.5 41.0 68-69 32.70546333751278 36.0 32.0 39.0 19.5 40.5 70-71 32.23805348646481 35.5 31.5 39.0 18.0 40.0 72-73 31.783385707779104 35.0 31.0 38.0 14.5 40.0 74-75 31.287875428356188 35.0 31.0 37.0 10.0 39.0 76-77 30.80133933763584 35.0 30.5 37.0 7.5 39.0 78-79 30.295108616946962 34.0 30.0 36.0 6.0 39.0 80-81 29.792635837777283 34.0 29.0 36.0 2.0 37.5 82-83 29.307354895397424 34.0 29.0 35.0 2.0 37.0 84-85 28.769232414229343 34.0 29.0 35.0 2.0 36.5 86-87 28.31556672827042 34.0 27.5 35.0 2.0 36.0 88-89 27.845002967635146 33.5 26.5 35.0 2.0 36.0 90-91 27.333925922424292 33.0 26.0 35.0 2.0 35.0 92-93 26.824387470351787 33.0 25.0 35.0 2.0 35.0 94-95 26.273905407849114 32.5 24.5 35.0 2.0 35.0 96-97 25.53315516267218 32.0 21.5 34.0 2.0 35.0 98-99 24.60350331017002 32.0 10.0 34.0 2.0 35.0 100-101 22.0225874176772 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04314250506394046 1101 2 8.332083119988454E-4 1101 3 -0.01806167835618311 1101 4 -0.12669230028534884 1101 5 -0.15894208702378165 1101 6 -0.10955299685583952 1101 7 -0.09023540825401 1101 8 -0.16407111090246929 1101 9 -0.21852084474812727 1101 10-11 -0.14147856945303516 1101 12-13 -0.10963116278745844 1101 14-15 -0.13831512505001342 1101 16-17 -0.2091767149512691 1101 18-19 -0.09404288733789201 1101 20-21 -0.004025871779965939 1101 22-23 -0.13184668727610216 1101 24-25 -0.18476061478906303 1101 26-27 -0.2270231861569343 1101 28-29 -0.16407025183364965 1101 30-31 -0.18961947890941389 1101 32-33 -0.38600077384811726 1101 34-35 -0.22188709035589937 1101 36-37 -0.10764037494190859 1101 38-39 -0.1405293934149512 1101 40-41 -0.18284065067797428 1101 42-43 -0.13222312562301397 1101 44-45 -0.18885076059992656 1101 46-47 -0.06937059846844562 1101 48-49 -0.22070765493121058 1101 50-51 -0.23210048245021397 1101 52-53 -0.06723624302463804 1101 54-55 -0.12170851415582717 1101 56-57 -0.0685456976204577 1101 58-59 -0.17827155325777966 1101 60-61 0.02060729030932862 1101 62-63 0.014072711853721387 1101 64-65 -0.1446899409521265 1101 66-67 -0.12570939991160657 1101 68-69 -0.2651455801546234 1101 70-71 -0.15804966347140947 1101 72-73 -0.11196181552823958 1101 74-75 -0.17097546266885644 1101 76-77 -0.09576893768102579 1101 78-79 -0.07681294735822775 1101 80-81 -0.0157266329974739 1101 82-83 0.15428821768083978 1101 84-85 0.1461864499665424 1101 86-87 -0.031557959252381806 1101 88-89 0.07941543404992046 1101 90-91 0.05144218502500664 1101 92-93 0.10009753247958031 1101 94-95 0.14208995202419672 1101 96-97 0.266527980739081 1101 98-99 0.35430948434683174 1101 100-101 0.5741770960928747 1102 1 -0.1687098050957161 1102 2 -0.07419766555071661 1102 3 -0.033179989147182454 1102 4 -0.1770297445051625 1102 5 -0.045237142907581074 1102 6 -0.2091331967511465 1102 7 -0.06588208999495038 1102 8 -0.18103206745877998 1102 9 -0.2149305349579862 1102 10-11 -0.22020447287329858 1102 12-13 -0.1580456584977341 1102 14-15 -0.09264929537395972 1102 16-17 -0.023959011045612044 1102 18-19 -0.06451152916423553 1102 20-21 -0.07777871355688859 1102 22-23 -0.14243494469312168 1102 24-25 -0.03265512864373932 1102 26-27 -0.10842106984863165 1102 28-29 -0.09552356072885004 1102 30-31 -0.09760836431883746 1102 32-33 -0.16873455173438145 1102 34-35 -0.022624225414951127 1102 36-37 0.07085348520812573 1102 38-39 -0.08940019483825523 1102 40-41 -0.1095943781836155 1102 42-43 -0.20064312643659576 1102 44-45 0.035535302374306355 1102 46-47 0.2245026187530712 1102 48-49 0.01734695208615733 1102 50-51 0.09441848665059638 1102 52-53 -0.02821088424310858 1102 54-55 -0.07622948190271472 1102 56-57 0.03756540837380129 1102 58-59 0.022171291911618596 1102 60-61 0.14404225026733286 1102 62-63 0.16677998887497125 1102 64-65 0.09575974098376605 1102 66-67 -0.036543899889345255 1102 68-69 -0.0405288266804007 1102 70-71 -0.19115434985130264 1102 72-73 -0.08623375650217113 1102 74-75 -0.13685077793147826 1102 76-77 -0.13128827097444784 1102 78-79 -0.19445857835167146 1102 80-81 -0.09617703065436345 1102 82-83 -0.2542686873189943 1102 84-85 -0.07605077105577962 1102 86-87 0.07770064014495404 1102 88-89 -0.21118657365300209 1102 90-91 -0.14573242480151194 1102 92-93 -0.12204494665513721 1102 94-95 -0.07345714855743424 1102 96-97 -0.016860115803815745 1102 98-99 0.007349725136101881 1102 100-101 0.11178727491183338 1103 1 -0.03550329891909243 1103 2 -0.0214597353766095 1103 3 -0.10722400250349295 1103 4 -0.042666848450267025 1103 5 -0.06204168884165995 1103 6 -0.1599397915121159 1103 7 -0.1003131825965724 1103 8 -0.12135872849891882 1103 9 0.023855968405371186 1103 10-11 0.045740865647822204 1103 12-13 -0.035235671910974986 1103 14-15 -0.21737869658399944 1103 16-17 -0.01882684412859703 1103 18-19 -0.06707999364091677 1103 20-21 -0.10841587611167114 1103 22-23 -0.10901256651333568 1103 24-25 -0.10386211960612002 1103 26-27 -0.10528519042716056 1103 28-29 -0.17012258680346548 1103 30-31 -0.32906625075337814 1103 32-33 -0.28338743173022607 1103 34-35 -0.14933332548846323 1103 36-37 -0.1729747587119519 1103 38-39 -0.17255220448038244 1103 40-41 -0.015540706789657577 1103 42-43 -0.013582332710846856 1103 44-45 -0.11185437955933963 1103 46-47 -0.030010221790092828 1103 48-49 -0.026125677612874654 1103 50-51 -0.18126976889094948 1103 52-53 -0.12103631657321046 1103 54-55 0.029996006692677213 1103 56-57 -0.03776958779982209 1103 58-59 -0.07650741569474206 1103 60-61 -0.021250071781125257 1103 62-63 0.0786137119626602 1103 64-65 0.020941399587421472 1103 66-67 0.015949125558982757 1103 68-69 0.11946188368564492 1103 70-71 -0.007668798811486965 1103 72-73 0.04980443440127402 1103 74-75 0.1484273815069379 1103 76-77 0.11167438126419071 1103 78-79 0.2067406803196299 1103 80-81 0.14816849600383009 1103 82-83 0.04334063438282598 1103 84-85 -0.10713598366656996 1103 86-87 0.15694997785559295 1103 88-89 0.23420459152413997 1103 90-91 0.27331149226746376 1103 92-93 0.18413310996515975 1103 94-95 -0.08677384302183455 1103 96-97 0.08818328884912319 1103 98-99 0.5178625221406428 1103 100-101 0.7897297109979746 1104 1 0.05433581704737378 1104 2 0.05510600644463892 1104 3 -0.023089893937950023 1104 4 -0.056021208504965614 1104 5 0.03862242978732411 1104 6 0.08893799551088932 1104 7 0.05180990687349407 1104 8 0.007295646761996011 1104 9 0.19759124699519504 1104 10-11 0.10700607969381082 1104 12-13 0.1221659105605255 1104 14-15 0.21142882291783138 1104 16-17 0.07690516427069838 1104 18-19 0.11919209737312286 1104 20-21 0.12330280777047875 1104 22-23 0.05750255057166953 1104 24-25 0.1344471770864999 1104 26-27 0.21001072422668443 1104 28-29 0.11002925892920246 1104 30-31 0.06388461494726982 1104 32-33 0.0498470327290903 1104 34-35 0.04683305670238269 1104 36-37 0.06671317786161524 1104 38-39 0.2531072867891382 1104 40-41 0.31117171520730125 1104 42-43 0.2674306859734301 1104 44-45 0.2329958107461536 1104 46-47 0.21946909348600485 1104 48-49 0.33236055791488184 1104 50-51 0.2667328349598108 1104 52-53 0.279275951880841 1104 54-55 0.21267419135535448 1104 56-57 0.15201536670397076 1104 58-59 0.33435813687462 1104 60-61 0.3645736352207365 1104 62-63 0.4017206093765253 1104 64-65 0.5325262573865288 1104 66-67 0.5002843320474213 1104 68-69 0.4645545761026426 1104 70-71 0.39092636333403874 1104 72-73 0.40190012990336754 1104 74-75 0.4411540267254388 1104 76-77 0.40694063525581825 1104 78-79 0.5019941200850795 1104 80-81 0.43618747928325874 1104 82-83 0.5471567678922575 1104 84-85 0.7529889920575918 1104 86-87 0.7502009242573884 1104 88-89 0.6568062667136907 1104 90-91 0.7659332316876153 1104 92-93 0.781852918082091 1104 94-95 1.0188337762600987 1104 96-97 1.0002529230627673 1104 98-99 1.1032196774052103 1104 100-101 1.423347178439876 1105 1 0.039611218872224185 1105 2 0.022787610307513972 1105 3 0.02424491302011944 1105 4 0.008613253927798326 1105 5 -0.03565349916416238 1105 6 -0.07132888783103652 1105 7 -0.028829401629550944 1105 8 -0.14469214486863535 1105 9 -0.07094628238264988 1105 10-11 -0.09538317274742525 1105 12-13 -0.06916358435896797 1105 14-15 0.023549240774904945 1105 16-17 -0.17967853091613506 1105 18-19 -0.04170034840709036 1105 20-21 -0.13917975482215894 1105 22-23 -0.17868891580143043 1105 24-25 -0.07646405304068082 1105 26-27 -0.061865176766708885 1105 28-29 -0.23124533910033307 1105 30-31 -0.22940602404088395 1105 32-33 -0.09912070187866107 1105 34-35 -0.10924448042218415 1105 36-37 -0.0037549164154242476 1105 38-39 -0.04412340537763981 1105 40-41 -0.036050094438827784 1105 42-43 -0.050157869845200764 1105 44-45 -0.09459679317205882 1105 46-47 0.047742593577744685 1105 48-49 0.03746232830318519 1105 50-51 -0.22823732850768863 1105 52-53 -0.11974755330741971 1105 54-55 0.07592385425642334 1105 56-57 0.05701870128959996 1105 58-59 0.15135636910739692 1105 60-61 0.2406947986270893 1105 62-63 0.2416846715716403 1105 64-65 0.19790772524491018 1105 66-67 0.07078766517689417 1105 68-69 2.585231453480219E-4 1105 70-71 0.029487278495771818 1105 72-73 0.1737335112896794 1105 74-75 0.23344196018516783 1105 76-77 0.3269664964962864 1105 78-79 0.3731623317278796 1105 80-81 0.33520253879614614 1105 82-83 0.42075587789281244 1105 84-85 0.5519254194204102 1105 86-87 0.4485965371658729 1105 88-89 0.3981892023722047 1105 90-91 0.6017327022311356 1105 92-93 0.8161962376984846 1105 94-95 0.8688985749763596 1105 96-97 0.8892147165747417 1105 98-99 0.8472129092157026 1105 100-101 1.0399968081406925 1106 1 -0.2291116165134035 1106 2 -0.1989162462377827 1106 3 -0.23049737108024715 1106 4 0.049829283795467916 1106 5 -0.17440307142523181 1106 6 -0.27997151259035036 1106 7 -0.5336397409095213 1106 8 -0.35068546958760294 1106 9 -0.5259717679621048 1106 10-11 -0.819437997387233 1106 12-13 -0.43406817942426557 1106 14-15 -0.5872852587322441 1106 16-17 -0.2719397121395559 1106 18-19 -0.5657387019058717 1106 20-21 -0.44605971212488527 1106 22-23 -0.47169461813599867 1106 24-25 -0.33749314184183987 1106 26-27 -0.47058696514957177 1106 28-29 -0.16446852433810477 1106 30-31 -0.4506770343219344 1106 32-33 -0.28849553908249703 1106 34-35 -0.2648469028290634 1106 36-37 -0.3881335713488525 1106 38-39 -0.3754675398638696 1106 40-41 -0.2764206695753231 1106 42-43 -0.22400508149808473 1106 44-45 -0.02633680961766771 1106 46-47 -0.15913372475196041 1106 48-49 0.11127410785036318 1106 50-51 0.012503783460310558 1106 52-53 0.07935911073747803 1106 54-55 -0.20977217275114413 1106 56-57 -0.05807748251301348 1106 58-59 -0.006644200359964714 1106 60-61 -0.15621877767898695 1106 62-63 -0.22074778398181394 1106 64-65 -0.004393701743246936 1106 66-67 0.009559965058912212 1106 68-69 -0.1632497915887967 1106 70-71 0.09757725178443621 1106 72-73 0.03446375741193819 1106 74-75 0.1610500185313093 1106 76-77 0.18512207344588205 1106 78-79 0.24982132037428073 1106 80-81 0.03876860467049781 1106 82-83 0.13899358071767764 1106 84-85 -0.16612248223283288 1106 86-87 -0.023883888989786328 1106 88-89 0.37412684441983757 1106 90-91 0.5690329545332666 1106 92-93 0.6144363154320516 1106 94-95 0.620150573219437 1106 96-97 0.6852967556253873 1106 98-99 0.6591565293937443 1106 100-101 0.9871609628519522 1107 1 0.015304298444235087 1107 2 0.01620467419181537 1107 3 0.031133283734526174 1107 4 0.006957470197377802 1107 5 0.012250502016357245 1107 6 -0.043532431177688125 1107 7 0.07704242269657868 1107 8 0.054593435845099236 1107 9 0.1304421029610694 1107 10-11 0.08475734497758225 1107 12-13 0.17503188615152254 1107 14-15 0.2132488055846622 1107 16-17 0.08030107709461731 1107 18-19 0.1672723176769466 1107 20-21 0.154197491890379 1107 22-23 0.13537087101768464 1107 24-25 0.17062172434189193 1107 26-27 0.19865393483967608 1107 28-29 0.17079589520654537 1107 30-31 0.1469014866480549 1107 32-33 0.15671276820693691 1107 34-35 0.22048120089033318 1107 36-37 0.11622357213491341 1107 38-39 0.1668742119526243 1107 40-41 0.25118901085787826 1107 42-43 0.3472193488808273 1107 44-45 0.17529830829812454 1107 46-47 0.2080721601811817 1107 48-49 0.09650941306286853 1107 50-51 0.12548233316083923 1107 52-53 0.019947368285826883 1107 54-55 0.060581267565019914 1107 56-57 0.16575621707971067 1107 58-59 0.15364184932411007 1107 60-61 0.3451786828494363 1107 62-63 0.323969719269563 1107 64-65 0.40191914628542236 1107 66-67 0.22195642001887705 1107 68-69 0.1295196283835338 1107 70-71 0.4202785080322329 1107 72-73 0.5592010704716017 1107 74-75 0.41536699958889756 1107 76-77 0.46020823761343266 1107 78-79 0.5244972246731159 1107 80-81 0.8162432680737233 1107 82-83 0.7040787798904127 1107 84-85 0.6916413945022413 1107 86-87 0.7810950456120729 1107 88-89 0.7610386362178225 1107 90-91 0.8752670677578607 1107 92-93 0.9206893447481548 1107 94-95 1.2817171600547148 1107 96-97 1.1808114487680044 1107 98-99 1.2969542248106762 1107 100-101 1.6721899455938996 1108 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-0.013547626791847733 2316 5 0.004494153440866455 2316 6 -0.10018501314090145 2316 7 -0.07046765274723299 2316 8 -0.4054840997501543 2316 9 -0.06641603200107227 2316 10-11 -0.6001531962376063 2316 12-13 -0.09838288744732893 2316 14-15 -0.12974858665341316 2316 16-17 -0.10841233405321304 2316 18-19 0.004967725505807152 2316 20-21 0.03636988308254985 2316 22-23 0.03827706131457376 2316 24-25 0.11688021351339728 2316 26-27 0.012478559363792385 2316 28-29 0.27380667488552746 2316 30-31 0.1267122495927353 2316 32-33 -0.008838278384146747 2316 34-35 0.03129016489763359 2316 36-37 -0.19776978992563699 2316 38-39 -0.10521088196863815 2316 40-41 -0.10587007517037961 2316 42-43 -0.12991950398282626 2316 44-45 -0.23054056827245972 2316 46-47 -0.42246654486281443 2316 48-49 -0.21927242743134912 2316 50-51 -0.2705321141191419 2316 52-53 -0.10311877403940883 2316 54-55 -0.21125072005159495 2316 56-57 -0.18113120290940543 2316 58-59 -0.01940267786215344 2316 60-61 -0.0802672165495224 2316 62-63 -0.06767568088941545 2316 64-65 0.03002649682719749 2316 66-67 -0.11407253032208331 2316 68-69 -0.05399569047463615 2316 70-71 -0.0728590339778421 2316 72-73 -0.12727970599996397 2316 74-75 0.043061570702704444 2316 76-77 0.09702300284513043 2316 78-79 0.19976253307233804 2316 80-81 0.20611692410016502 2316 82-83 -0.08542951056127635 2316 84-85 0.0057888515857307254 2316 86-87 0.017252292261726865 2316 88-89 -0.0014416661152196752 2316 90-91 -0.013701541482259216 2316 92-93 0.008833092394894493 2316 94-95 0.13912557461913266 2316 96-97 0.20207586467116911 2316 98-99 0.1994141670773395 2316 100-101 0.26700998213045324 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10421.0 3 2497.0 4 1961.0 5 2125.0 6 2493.0 7 2910.0 8 3577.0 9 4640.0 10 5897.0 11 7529.0 12 9643.0 13 12472.0 14 13911.0 15 17125.0 16 21329.0 17 27398.0 18 24074.0 19 17326.0 20 17485.0 21 18453.0 22 20314.0 23 22424.0 24 24916.0 25 28740.0 26 33202.0 27 39044.0 28 46129.0 29 55712.0 30 67180.0 31 83842.0 32 105430.0 33 136207.0 34 182654.0 35 254919.0 36 369302.0 37 513062.0 38 410508.0 39 48555.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32669202847864 19.139969824558236 14.833764303625715 22.699573843337408 2 14.961816161133093 20.308568730158015 40.87283683815557 23.856778270553328 3 18.944715475884802 24.736776612657955 30.12342467282932 26.195083238627927 4 12.15800980135986 16.779085920743203 38.35128870775819 32.71161557013875 5 13.269988051796103 37.586124443288135 34.71817954187774 14.425707963038018 6 31.065443784801573 36.613614031076466 18.103793533577793 14.21714865054417 7 28.07114528780552 32.90890752661969 21.15062791402576 17.86931927154903 8 25.99002641173427 34.738499765362725 20.301689388378836 18.96978443452417 9 26.04663929371776 16.789544135120096 20.91368497044624 36.2501316007159 10-11 24.35333299113422 26.842060632793846 28.017909422903763 20.78669695316817 12-13 25.579792317745913 24.262707691185323 28.378503136179912 21.778996854888856 14-15 22.357726557728025 25.534010096743064 26.985391062203462 25.12287228332545 16-17 21.55869765951674 28.936750601718487 27.503738354131425 22.000813384633346 18-19 21.393519242685855 27.432427249592408 30.00482420782221 21.16922929989953 20-21 22.34059032779236 26.449265145183126 29.96223926462256 21.247905262401957 22-23 21.942418791260458 26.531255633849547 29.871250734809074 21.655074840080918 24-25 21.77563327861176 26.60132034828561 30.014040378919166 21.60900599418346 26-27 21.582076100772117 26.819693383833815 29.929582694228497 21.668647821165575 28-29 21.35439169638234 27.026447231318983 30.04791358558095 21.571247486717724 30-31 21.575429519021423 26.68276697112474 29.987948273463267 21.753855236390574 32-33 21.40165594801501 26.76297472007605 29.916431743754096 21.918937588154847 34-35 21.885543094090632 26.792646818534543 29.73765740546271 21.58415268191212 36-37 21.82581764006448 26.573511234766915 29.77449630364184 21.826174821526763 38-39 21.47863308919736 26.977820973636323 29.832801949077346 21.71074398808897 40-41 21.800174187240568 26.836471341724877 29.73887140729417 21.624483063740385 42-43 21.585431857715996 26.84691858922914 29.49933760492152 22.068311948133346 44-45 21.760138082488492 26.968389940134173 29.246694594025453 22.024777383351886 46-47 21.925019766524674 27.202686555096662 28.90820153299858 21.964092145380086 48-49 21.90910743890852 27.185936328612637 28.845159078810873 22.059797153667972 50-51 21.77501392394162 27.386559833085695 28.7661676611802 22.072258581792486 52-53 22.116852592054038 27.498915816590397 28.40660206575963 21.977629525595944 54-55 21.89758293068902 27.265758034333576 28.571863736739818 22.26479529823759 56-57 21.96111686877606 27.534181448972728 28.396897515715352 22.107804166535857 58-59 22.189909603821025 27.195541861120358 28.441217108904905 22.173331426153712 60-61 22.06559930772409 27.215698303630802 28.792810044015305 21.925892344629805 62-63 21.91042440822377 27.325718983850315 28.801364951373515 21.962491656552398 64-65 21.728752917047586 27.389667185921184 28.700665808593563 22.180914088437664 66-67 21.740366860737872 27.442398894515886 28.44142452875582 22.375809715990425 68-69 21.78640498585478 27.285589128620114 28.621561224253885 22.306444661271225 70-71 21.782280388250534 27.251895756021465 28.58861920467624 22.37720465105176 72-73 21.818877984018567 27.13326512594151 28.65321353243175 22.39464335760817 74-75 21.688156330111607 27.493824157572234 28.403198392758387 22.414821119557768 76-77 21.669134708857186 27.273805672179453 28.47100509443763 22.586054524525736 78-79 21.764971714028015 27.307195017367853 28.435757739588592 22.492075529015537 80-81 21.73779091176869 27.519982164974362 28.339664003004582 22.402562920252358 82-83 21.861599655191792 27.33620059476784 28.42983508265604 22.37236466738433 84-85 21.83446613174296 27.275751873445376 28.25182128382817 22.637960710983492 86-87 21.710579989721122 27.26470896747641 28.48691435482737 22.5377966879751 88-89 21.882219412623417 27.12961511378687 28.193421704113153 22.794743769476554 90-91 21.754967553482377 27.096089780573596 28.463996387541457 22.68494627840257 92-93 21.84978267362848 26.90064943206415 28.573300257393075 22.6762676369143 94-95 21.69729375937389 26.853683004938194 28.55828132639268 22.890741909295233 96-97 21.611046025482153 26.888903593729317 28.64918578630124 22.850864594487287 98-99 21.559179081806068 26.90866788787723 28.39196121603696 23.140191814279742 100-101 21.685498768125694 26.488774794430743 28.925194366985018 22.90053207045855 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3547.0 1 2064.5 2 571.0 3 504.0 4 400.0 5 380.0 6 539.5 7 1029.0 8 1726.5 9 2160.0 10 2167.5 11 2110.5 12 2306.0 13 2556.5 14 2781.0 15 3048.0 16 3176.0 17 3335.5 18 3734.0 19 4072.0 20 4476.5 21 5248.5 22 6219.0 23 7572.0 24 9352.0 25 11756.5 26 15146.5 27 19486.0 28 23495.5 29 27793.0 30 33316.5 31 39175.5 32 45289.0 33 51527.5 34 57951.0 35 63997.5 36 70685.5 37 77372.5 38 81582.0 39 84282.0 40 86543.0 41 89439.5 42 92653.0 43 96662.0 44 100982.0 45 103356.5 46 102477.0 47 104000.5 48 111392.5 49 110807.0 50 102955.0 51 95772.0 52 88413.5 53 83606.0 54 78586.5 55 70849.5 56 62792.5 57 55537.0 58 49291.0 59 43488.5 60 37042.0 61 29961.0 62 23342.0 63 17853.0 64 13629.5 65 10510.5 66 7719.0 67 5688.5 68 4070.0 69 2640.0 70 1866.0 71 1388.5 72 1065.5 73 811.5 74 612.5 75 486.5 76 339.0 77 206.0 78 127.5 79 90.5 80 59.0 81 37.0 82 29.0 83 20.5 84 13.5 85 9.5 86 8.0 87 7.5 88 5.5 89 5.5 90 4.0 91 3.0 92 2.5 93 1.5 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23658542624770112 2 0.21227407892633887 3 0.20330739372602163 4 0.20961033562415257 5 0.21039820336141896 6 0.23407175299070843 7 0.2363603211799107 8 0.22503003276779435 9 0.21992765123121216 10-11 0.23078897075209853 12-13 0.23319009147519604 14-15 0.21953371736257898 16-17 0.23123918088767934 18-19 0.2220286318639225 20-21 0.2168887328160419 22-23 0.21450637084859359 24-25 0.20985419944759215 26-27 0.2167574215264975 28-29 0.22701846086660948 30-31 0.22705597837790792 32-33 0.22656825073102868 34-35 0.2343531343254464 36-37 0.2142062307582064 38-39 0.21392484942346837 40-41 0.2116175224786169 42-43 0.21593203627793273 44-45 0.23043255439476376 46-47 0.23465327441583358 48-49 0.2369418426050359 50-51 0.23759839905275787 52-53 0.2528305086399077 54-55 0.23763591656405628 56-57 0.2131932579531496 58-59 0.1987865336145646 60-61 0.19550375137595472 62-63 0.20261331976700125 64-65 0.19501602372907553 66-67 0.1962165840906243 68-69 0.20021219904390375 70-71 0.20017468153260537 72-73 0.20250076723310606 74-75 0.20488312920055435 76-77 0.2159695537892311 78-79 0.21096096603089493 80-81 0.20587734324996193 82-83 0.20231317967661405 84-85 0.20321359994777563 86-87 0.21006054575973335 88-89 0.20608368956210313 90-91 0.21525672107456156 92-93 0.20568975569346992 94-95 0.20411402021893718 96-97 0.21302442915230685 98-99 0.21632597014656593 100-101 0.19689189929399548 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2665422.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.26400964471326 #Duplication Level Percentage of deduplicated Percentage of total 1 76.02290319469428 42.013304553706874 2 13.29699925692057 14.696909903604066 3 4.627958870527263 7.672786909684648 4 2.0620194612184166 4.55821853569444 5 1.0984258502235065 3.0351708390377112 6 0.6607484920869034 2.190936662365223 7 0.43495428676626097 1.682612252920202 8 0.2927971190279584 1.2944914247924277 9 0.21836453721209886 1.0860929901497502 >10 1.1432192698751458 11.51762227560183 >50 0.09311342593753703 3.5430089826544475 >100 0.045843289317594654 4.488192444800266 >500 0.0018366550563200888 0.6796426601206589 >1k 6.802426134385133E-4 0.8791859556390287 >5k 1.3604852268770266E-4 0.6618236092284218 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9832 0.36887217108585435 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7773 0.2916236153224518 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4863 0.18244765744411204 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3768 0.14136598257236566 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3503 0.13142384207829005 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.7517511298398524E-5 2 0.0 0.0 0.0 0.0 3.7517511298398524E-5 3 0.0 0.0 0.0 0.0 3.7517511298398524E-5 4 0.0 0.0 0.0 0.0 3.7517511298398524E-5 5 0.0 0.0 0.0 7.503502259679705E-5 3.7517511298398524E-5 6 0.0 0.0 0.0 7.503502259679705E-5 3.7517511298398524E-5 7 0.0 0.0 0.0 7.503502259679705E-5 3.7517511298398524E-5 8 0.0 0.0 0.0 1.1255253389519558E-4 3.7517511298398524E-5 9 0.0 0.0 0.0 1.500700451935941E-4 3.7517511298398524E-5 10-11 0.0 0.0 0.0 1.8758755649199264E-4 9.379377824599631E-5 12-13 0.0 0.0 0.0 2.2510506779039116E-4 1.500700451935941E-4 14-15 3.7517511298398524E-5 0.0 0.0 2.81381334737989E-4 1.500700451935941E-4 16-17 7.503502259679705E-5 0.0 0.0 4.3145137993158306E-4 1.8758755649199264E-4 18-19 1.1255253389519558E-4 0.0 0.0 5.252451581775794E-4 1.8758755649199264E-4 20-21 1.1255253389519558E-4 0.0 0.0 6.190389364235758E-4 1.8758755649199264E-4 22-23 1.1255253389519558E-4 0.0 0.0 7.691089816171698E-4 1.8758755649199264E-4 24-25 1.1255253389519558E-4 0.0 0.0 9.566965381091624E-4 1.8758755649199264E-4 26-27 1.1255253389519558E-4 0.0 0.0 0.0012943541397947493 1.8758755649199264E-4 28-29 1.500700451935941E-4 0.0 0.0 0.0023073269448515097 1.8758755649199264E-4 30-31 1.500700451935941E-4 0.0 0.0 0.004877276468791809 1.8758755649199264E-4 32-33 1.500700451935941E-4 0.0 0.0 0.009004202711615648 1.8758755649199264E-4 34-35 1.500700451935941E-4 0.0 0.0 0.013675132868266264 1.8758755649199264E-4 36-37 1.500700451935941E-4 0.0 0.0 0.01870247938225167 1.8758755649199264E-4 38-39 1.8758755649199264E-4 0.0 0.0 0.024105001009221055 1.8758755649199264E-4 40-41 1.8758755649199264E-4 0.0 0.0 0.028419514808536883 1.8758755649199264E-4 42-43 1.8758755649199264E-4 0.0 0.0 0.03410341777024426 1.8758755649199264E-4 44-45 1.8758755649199264E-4 0.0 0.0 0.040593947224867206 1.8758755649199264E-4 46-47 2.2510506779039116E-4 0.0 0.0 0.0510425741214712 1.8758755649199264E-4 48-49 2.2510506779039116E-4 0.0 0.0 0.06428625560980587 2.063463121411919E-4 50-51 2.2510506779039116E-4 0.0 0.0 0.07726731451905178 2.2510506779039116E-4 52-53 2.2510506779039116E-4 0.0 0.0 0.0992900936512117 2.2510506779039116E-4 54-55 2.2510506779039116E-4 0.0 0.0 0.14089701368113566 2.2510506779039116E-4 56-57 2.2510506779039116E-4 0.0 0.0 0.20722797365670426 2.2510506779039116E-4 58-59 2.2510506779039116E-4 0.0 0.0 0.27614764191186236 2.2510506779039116E-4 60-61 2.2510506779039116E-4 0.0 0.0 0.34487972261052846 2.2510506779039116E-4 62-63 2.2510506779039116E-4 0.0 0.0 0.42957550436666314 2.2510506779039116E-4 64-65 2.2510506779039116E-4 0.0 0.0 0.5313605125192183 2.626225790887897E-4 66-67 2.2510506779039116E-4 0.0 0.0 0.6818432503370948 2.626225790887897E-4 68-69 2.2510506779039116E-4 0.0 0.0 0.860726744207859 2.626225790887897E-4 70-71 2.2510506779039116E-4 0.0 0.0 1.0285988485125432 2.626225790887897E-4 72-73 2.2510506779039116E-4 0.0 0.0 1.2116280273817805 2.626225790887897E-4 74-75 2.2510506779039116E-4 0.0 0.0 1.4096829695260262 3.001400903871882E-4 76-77 3.001400903871882E-4 0.0 0.0 1.639909177608649 3.001400903871882E-4 78-79 3.001400903871882E-4 0.0 0.0 1.8548469998371742 3.001400903871882E-4 80-81 3.751751129839853E-4 0.0 0.0 2.0890500641174268 3.001400903871882E-4 82-83 4.126926242823838E-4 0.0 0.0 2.3549554254448264 3.001400903871882E-4 84-85 4.126926242823838E-4 0.0 0.0 2.6969650584410276 3.3765760168558677E-4 86-87 4.126926242823838E-4 0.0 0.0 3.08836649506157 3.3765760168558677E-4 88-89 4.126926242823838E-4 0.0 0.0 3.551238790705562 3.3765760168558677E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9760 0.0 30.085546 1 GTATCAA 14105 0.0 29.643454 1 GTACATG 11470 0.0 24.68892 1 ATCAACG 16785 0.0 24.680683 3 TCAACGC 16810 0.0 24.645369 4 CAACGCA 16830 0.0 24.587852 5 TATCAAC 16955 0.0 24.4888 2 TACATGG 11250 0.0 24.154463 2 AACGCAG 17180 0.0 24.111868 6 ACATGGG 11205 0.0 23.234362 3 GAGTACT 9175 0.0 22.468826 12-13 ACGCAGA 19210 0.0 21.415493 7 CGCAGAG 19380 0.0 21.203125 8 GTGTAGC 1450 0.0 20.643902 1 GTACTTT 9815 0.0 20.518602 14-15 AGTACTT 9715 0.0 20.193144 12-13 AGAGTAC 15470 0.0 20.035454 10-11 GCAGAGT 20515 0.0 19.82165 9 GTACCTG 2110 0.0 19.140608 1 GTTCGCG 175 9.9353034E-5 19.00192 7 >>END_MODULE