FastQCFastQC Report
Thu 26 May 2016
SRR937408_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937408_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665422
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111730.41918315373700676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91660.3438855085611209No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61140.2293820640784086No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46240.1734809722437948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA163900.026.9759941
GGTATCA116950.025.772851
CAACGCA194400.022.699165
ATCAACG194000.022.672513
TCAACGC194500.022.6386494
GTACATG114750.022.496791
TATCAAC197900.022.3217122
GAGTACT102450.022.27777312-13
AACGCAG198800.022.2202426
TACATGG115900.021.6391832
ACATGGG113350.021.2459813
GTACTTT109450.020.78748514-15
AGTACTT110800.020.51315912-13
AGAGTAC168750.020.30873710-11
ACGCAGA222800.019.8057337
CGCAGAG224400.019.5586788
ACCGTCG1559.6295483E-418.386968
GCAGAGT238300.018.178639
TAGCCCT17500.017.914154
TACTTTT127600.017.8678714-15
Produced by FastQC (version 0.11.5)