##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937408_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2665422 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20454734747443 33.0 31.0 34.0 31.0 34.0 2 32.40496701835582 34.0 31.0 34.0 31.0 34.0 3 32.45973995862569 34.0 31.0 34.0 31.0 34.0 4 36.101204987427884 37.0 35.0 37.0 35.0 37.0 5 36.026597664459885 37.0 35.0 37.0 35.0 37.0 6 36.06616925950187 37.0 35.0 37.0 35.0 37.0 7 36.008636906276 37.0 35.0 37.0 35.0 37.0 8 35.98342138693235 37.0 35.0 37.0 35.0 37.0 9 37.696365153435366 39.0 37.0 39.0 35.0 39.0 10-11 37.61605610668779 39.0 37.0 39.0 35.0 39.0 12-13 37.52523746708776 39.0 37.0 39.0 35.0 39.0 14-15 38.735103484551416 40.0 38.0 41.0 34.5 41.0 16-17 38.708898253259704 40.0 38.0 41.0 34.5 41.0 18-19 38.70263095299731 40.0 38.0 41.0 34.5 41.0 20-21 38.60974509852473 40.0 38.0 41.0 34.0 41.0 22-23 38.461961745644786 40.0 38.0 41.0 34.0 41.0 24-25 38.403643025382095 40.0 38.0 41.0 34.0 41.0 26-27 38.36562315460741 40.0 38.0 41.0 34.0 41.0 28-29 38.248958138711245 40.0 38.0 41.0 34.0 41.0 30-31 38.327672316053516 40.0 38.0 41.0 34.0 41.0 32-33 38.28110389274194 40.0 38.0 41.0 34.0 41.0 34-35 38.1608786526111 40.0 38.0 41.0 34.0 41.0 36-37 38.006055701498674 40.0 37.5 41.0 33.0 41.0 38-39 37.891846206716984 40.0 37.0 41.0 33.0 41.0 40-41 37.728917409700976 40.0 37.0 41.0 33.0 41.0 42-43 37.52725328296982 40.0 37.0 41.0 33.0 41.0 44-45 37.254780856464755 40.0 37.0 41.0 32.0 41.0 46-47 36.92277789408206 40.0 36.5 41.0 31.0 41.0 48-49 36.62291243187758 40.0 36.0 41.0 31.0 41.0 50-51 36.39131420840677 39.0 36.0 41.0 30.0 41.0 52-53 36.16139189216567 39.0 35.5 41.0 30.0 41.0 54-55 35.93786987576451 39.0 35.0 40.0 29.0 41.0 56-57 35.68230227708783 39.0 35.0 40.0 28.0 41.0 58-59 35.427305132170446 38.0 35.0 40.0 28.0 41.0 60-61 35.11125930528074 38.0 34.0 40.0 27.5 41.0 62-63 34.760406607283954 38.0 34.0 40.0 26.5 41.0 64-65 34.37746030459717 37.0 34.0 40.0 26.0 41.0 66-67 33.974808304276024 36.5 33.5 39.0 26.0 41.0 68-69 33.55075725344805 36.0 33.0 39.0 25.5 40.0 70-71 33.15332412653606 35.5 32.5 39.0 25.0 40.0 72-73 32.69121324878387 35.0 32.0 38.0 23.5 40.0 74-75 32.2173743969998 35.0 31.5 37.0 22.5 39.0 76-77 30.929031500452837 34.0 30.5 36.0 21.5 38.5 78-79 31.537247760392162 34.5 31.0 36.0 22.0 39.0 80-81 31.342629234695295 34.5 31.0 36.0 20.5 37.5 82-83 30.999483759044534 34.0 31.0 35.5 20.0 37.0 84-85 30.638836176785514 34.0 31.0 35.0 18.5 37.0 86-87 30.235323712342737 34.0 30.5 35.0 17.5 36.0 88-89 29.78378864585045 34.0 30.0 35.0 10.5 36.0 90-91 29.289890869063136 34.0 29.0 35.0 6.5 35.0 92-93 28.952580679532172 34.0 29.0 35.0 2.0 35.0 94-95 28.524455602152305 34.0 29.0 35.0 2.0 35.0 96-97 28.067950966113433 33.5 29.0 35.0 2.0 35.0 98-99 27.34299296696733 33.0 27.5 35.0 2.0 35.0 100-101 24.871909026037905 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06844057122603431 1101 2 -0.03841498528545628 1101 3 -0.0016121205984944709 1101 4 -0.012359516186528197 1101 5 -0.06055200243324066 1101 6 -0.0731900257335596 1101 7 -0.0999087549952904 1101 8 -0.06565817061891721 1101 9 -0.06580063025388228 1101 10-11 -0.10788943257582417 1101 12-13 -0.11761063793885995 1101 14-15 -0.13838042557920716 1101 16-17 -0.12138447456350576 1101 18-19 -0.0627335480512059 1101 20-21 -0.05756416998818992 1101 22-23 -0.12725659191686844 1101 24-25 -0.17832589946147692 1101 26-27 -0.15669558323443056 1101 28-29 -0.200349792474654 1101 30-31 -0.13621670916985806 1101 32-33 -0.07140651755143068 1101 34-35 -0.010693037397459193 1101 36-37 -0.06729448373496894 1101 38-39 -0.07094202000028815 1101 40-41 0.12192597118792747 1101 42-43 0.11500679956490245 1101 44-45 0.062366211121165804 1101 46-47 0.10390033100018314 1101 48-49 0.12093816077231878 1101 50-51 0.3025676289401744 1101 52-53 0.3332750592560103 1101 54-55 0.17148218571723106 1101 56-57 0.2420887405101979 1101 58-59 0.3191325655187356 1101 60-61 0.22572203677690084 1101 62-63 0.05775327811935682 1101 64-65 0.11066343701997994 1101 66-67 0.14293031686189295 1101 68-69 0.13228900845856373 1101 70-71 0.0850017522147084 1101 72-73 0.11336086001158918 1101 74-75 0.0246039947959531 1101 76-77 0.10794484122198256 1101 78-79 -0.1789442895190696 1101 80-81 -0.23943813480928355 1101 82-83 0.058343761433533814 1101 84-85 0.04214327258807771 1101 86-87 0.044812789517465035 1101 88-89 0.034387539364761466 1101 90-91 -0.14842937218520902 1101 92-93 -0.07316825688539907 1101 94-95 -0.0203485554572147 1101 96-97 -0.11104649302337677 1101 98-99 0.11836381772279836 1101 100-101 0.7789391638035923 1102 1 -0.09385874033114305 1102 2 -0.09838367393108882 1102 3 -0.13449291870607283 1102 4 -0.049859842386446473 1102 5 -0.059825440089142035 1102 6 -0.11452655889619479 1102 7 -0.2059644070036839 1102 8 -0.15949581286127312 1102 9 -0.1565300669948968 1102 10-11 -0.1288154450619885 1102 12-13 -0.20865696830492197 1102 14-15 -0.19454902910584337 1102 16-17 -0.20641385558189995 1102 18-19 -0.2756069126383309 1102 20-21 -0.1642634432147716 1102 22-23 -0.09638412034998822 1102 24-25 -0.18211998825847076 1102 26-27 -0.17694621798109011 1102 28-29 -0.22698185700544116 1102 30-31 -0.14342246533197311 1102 32-33 -0.08256461744075949 1102 34-35 -0.0632045560921739 1102 36-37 -0.03537711708165858 1102 38-39 -0.009572158800267516 1102 40-41 0.02189211355538845 1102 42-43 0.051888362899106255 1102 44-45 0.00622754123394742 1102 46-47 -0.08929732619363762 1102 48-49 0.06299805184457341 1102 50-51 0.11329861800901853 1102 52-53 0.1475012804333673 1102 54-55 0.06488014884819648 1102 56-57 0.09938798173081409 1102 58-59 0.13436439388078014 1102 60-61 0.15474280573372567 1102 62-63 0.1247545971183186 1102 64-65 0.1387244970392274 1102 66-67 0.16428748888515088 1102 68-69 0.1630749411063377 1102 70-71 0.13041659983713316 1102 72-73 0.05719383951396395 1102 74-75 0.07697956487827895 1102 76-77 0.06778828828779382 1102 78-79 0.10549935157812484 1102 80-81 -0.024097883351359428 1102 82-83 -0.008631173748625542 1102 84-85 -0.020411078074527467 1102 86-87 -0.023874791169042453 1102 88-89 0.034975658626525075 1102 90-91 0.007944439871447884 1102 92-93 0.0241599163490136 1102 94-95 0.063938528868146 1102 96-97 0.09977526596937736 1102 98-99 0.2630501745839702 1102 100-101 0.7461727659256603 1103 1 -0.018909835411108133 1103 2 -0.009562904838148256 1103 3 -0.04339003527737617 1103 4 -0.02989278102545967 1103 5 0.0019978274506371463 1103 6 -0.04570215600322314 1103 7 -0.09466127942381064 1103 8 -0.10209171504004644 1103 9 -0.04152845941515437 1103 10-11 -0.11291097632523162 1103 12-13 -0.08782066262363486 1103 14-15 -0.19243390002221616 1103 16-17 -0.2371732611792794 1103 18-19 -0.18268911773535734 1103 20-21 -0.27734354296844543 1103 22-23 -0.1786240168787856 1103 24-25 -0.0929941348596941 1103 26-27 -0.12103797519970527 1103 28-29 -0.21970562247630454 1103 30-31 -0.269833702649251 1103 32-33 -0.13429849056922194 1103 34-35 -0.06504244046699625 1103 36-37 -0.05740572153498391 1103 38-39 -0.08092279580373685 1103 40-41 -0.01579061258168224 1103 42-43 -0.0777498604908402 1103 44-45 -0.15966448341225004 1103 46-47 -0.08031225324133118 1103 48-49 -0.14480475924304415 1103 50-51 -0.1118190095214473 1103 52-53 -0.06727812320477966 1103 54-55 -0.12602277091851022 1103 56-57 -0.24814919773703537 1103 58-59 -0.1868640524101437 1103 60-61 -0.23856858455481955 1103 62-63 -0.18531524677899114 1103 64-65 -0.27942771564357116 1103 66-67 -0.1653179549491952 1103 68-69 -0.05216685102690377 1103 70-71 -0.03190002513293422 1103 72-73 -0.03582725494641892 1103 74-75 0.0407097506207279 1103 76-77 0.17187970431436028 1103 78-79 0.07851922360807961 1103 80-81 -0.10814567912510142 1103 82-83 -0.13096186922781072 1103 84-85 -0.16004042000506757 1103 86-87 -0.0307886922961913 1103 88-89 0.02880929254377307 1103 90-91 0.05163805945445077 1103 92-93 0.11107273110391702 1103 94-95 0.1756549593378125 1103 96-97 0.2599834601047277 1103 98-99 0.3183511269075119 1103 100-101 0.9399055911968084 1104 1 0.04500752478995196 1104 2 0.03961194442383942 1104 3 -0.06139647200556908 1104 4 -0.03319065027593382 1104 5 0.0011776030706940332 1104 6 -0.030311529979861973 1104 7 -0.009126262699815868 1104 8 -0.0725564613952514 1104 9 0.011860846458148444 1104 10-11 -0.07691473288682715 1104 12-13 -0.13068988746546495 1104 14-15 -0.12408646249726729 1104 16-17 -0.13758730306528832 1104 18-19 -0.09721492587165415 1104 20-21 -0.1119727078055206 1104 22-23 -0.15720233258829097 1104 24-25 -0.008088495973701981 1104 26-27 -0.2627199192715892 1104 28-29 -0.13565312629351212 1104 30-31 -0.16906519079096682 1104 32-33 -0.21328743103774883 1104 34-35 -0.17967218621566872 1104 36-37 -0.15696855708973345 1104 38-39 -0.19168617627633466 1104 40-41 -0.1886356726405367 1104 42-43 -0.2000906380352987 1104 44-45 -0.1884764451791554 1104 46-47 -0.19966742998937548 1104 48-49 -0.08591695434276403 1104 50-51 -0.2477686661286711 1104 52-53 -0.19047808037679204 1104 54-55 -0.12937730752476284 1104 56-57 -0.14948125078679197 1104 58-59 -0.08328814292755737 1104 60-61 0.009586764872047127 1104 62-63 0.06516722307483036 1104 64-65 0.11016109577145983 1104 66-67 -0.054574735525790175 1104 68-69 -0.07295414569316705 1104 70-71 -0.021022113954316524 1104 72-73 0.11655439713195648 1104 74-75 0.05171455111460688 1104 76-77 0.09540969900072582 1104 78-79 -0.0528876474369504 1104 80-81 -0.1639214495306156 1104 82-83 -0.18461309788429503 1104 84-85 -0.03844543042445636 1104 86-87 -0.11969060423568934 1104 88-89 -0.09930534751030606 1104 90-91 -0.01491493078794548 1104 92-93 0.22646785634446687 1104 94-95 0.3204803754726875 1104 96-97 0.336321279556266 1104 98-99 0.653728129984632 1104 100-101 1.1959713617788772 1105 1 -0.019301342295506174 1105 2 -0.009550623177972284 1105 3 -0.08021867888966483 1105 4 -0.08181152973284611 1105 5 -0.06396534726582104 1105 6 -0.09355857180925398 1105 7 -0.12396647925858417 1105 8 -0.12106201369874015 1105 9 -0.11402165381124263 1105 10-11 -0.03975376122643581 1105 12-13 -0.05608195610259514 1105 14-15 -0.02135384966741327 1105 16-17 -0.07621694855077976 1105 18-19 -0.020769332038746313 1105 20-21 0.009366310341029305 1105 22-23 -0.05734882137155495 1105 24-25 -0.1361062959626338 1105 26-27 -0.002669419957435082 1105 28-29 -0.1291342298229381 1105 30-31 -0.1578740884555998 1105 32-33 -0.10756038944133905 1105 34-35 -0.21106738266498581 1105 36-37 -0.0744181961099244 1105 38-39 -0.16494134342790545 1105 40-41 -0.13956367762639843 1105 42-43 -0.15524817900411136 1105 44-45 -0.20211708460415423 1105 46-47 -0.23024426961764988 1105 48-49 -0.19693710455929647 1105 50-51 -0.2508306602982415 1105 52-53 -0.23405281441082337 1105 54-55 -0.07953471743998364 1105 56-57 -0.2921421044989643 1105 58-59 -0.31583544599043023 1105 60-61 -0.30292448389386806 1105 62-63 -0.12370603646078138 1105 64-65 -0.12169307945008967 1105 66-67 -0.10483494969646756 1105 68-69 -0.12450217974379996 1105 70-71 -0.1186788758823738 1105 72-73 -0.024790387372263467 1105 74-75 -0.0093060359471977 1105 76-77 0.1061106609340321 1105 78-79 -0.1142148998840149 1105 80-81 -0.33936229922380434 1105 82-83 -0.4749506627922173 1105 84-85 -0.38005610808797385 1105 86-87 -0.2820051270113453 1105 88-89 -0.041268122468004265 1105 90-91 0.13825962384829538 1105 92-93 0.3811999100123664 1105 94-95 0.4236390580638236 1105 96-97 0.24350680192128848 1105 98-99 0.3741724890015554 1105 100-101 0.8669173647902007 1106 1 -0.20421109576465568 1106 2 -0.06576394616044468 1106 3 -0.05573426390884606 1106 4 -0.028569779348515567 1106 5 -0.1104264779266586 1106 6 -0.09420439375750078 1106 7 -0.08144973998270189 1106 8 -0.12902778947670868 1106 9 -0.21065668907736068 1106 10-11 -0.25058142967896657 1106 12-13 -0.2282742011318888 1106 14-15 -0.1784818317100374 1106 16-17 -0.08948828314733248 1106 18-19 -0.12226475842423667 1106 20-21 -0.13232604194219988 1106 22-23 -0.16563495445837617 1106 24-25 -0.17710149762557847 1106 26-27 -0.10901297986897873 1106 28-29 -0.16423780648651842 1106 30-31 -0.19923114448811674 1106 32-33 -0.3117926427444999 1106 34-35 -0.17449851286616536 1106 36-37 -0.2152958653285495 1106 38-39 -0.29317224073480475 1106 40-41 -0.28217407257626803 1106 42-43 -0.3744434424657541 1106 44-45 -0.20288407796973473 1106 46-47 -0.3446738191000165 1106 48-49 -0.3533613602935546 1106 50-51 -0.19380597401601563 1106 52-53 -0.17654567691638334 1106 54-55 -0.210580456138743 1106 56-57 -0.31120464120790103 1106 58-59 -0.27829873705208996 1106 60-61 -0.23620997856436787 1106 62-63 -0.022334973574210437 1106 64-65 -0.2718561309779588 1106 66-67 -0.2325423456218374 1106 68-69 -0.23359942076383788 1106 70-71 -0.10424881566694921 1106 72-73 -0.10481510685213635 1106 74-75 -0.08270744148456544 1106 76-77 -0.010982361430972531 1106 78-79 -0.0669627075623751 1106 80-81 -0.09498464021983466 1106 82-83 -0.04448722361797053 1106 84-85 0.008534051618251937 1106 86-87 -0.0039646054507507245 1106 88-89 0.11477787159727626 1106 90-91 0.10427104128574172 1106 92-93 0.24877589432264813 1106 94-95 0.22522501188333877 1106 96-97 0.34976972985174726 1106 98-99 0.3518900995880294 1106 100-101 0.8316421179784506 1107 1 -0.04491485112969684 1107 2 -0.033385080424125135 1107 3 0.017022232809075888 1107 4 -0.01223477481962476 1107 5 0.017719898627476027 1107 6 -0.04942041003959474 1107 7 -0.01654013148548472 1107 8 -0.03133878756646169 1107 9 0.011428642782163934 1107 10-11 -0.011059573643031229 1107 12-13 -0.0744203079261112 1107 14-15 -0.002296944186760186 1107 16-17 0.06448911294575765 1107 18-19 0.008938348859061307 1107 20-21 0.009952436918929664 1107 22-23 0.01096128951862596 1107 24-25 -0.0019468517238507843 1107 26-27 -0.029680536962679582 1107 28-29 7.283309682151184E-4 1107 30-31 -0.0253584064688539 1107 32-33 0.058923410821485334 1107 34-35 0.10062671714253923 1107 36-37 -0.0358718027442535 1107 38-39 -0.02122984873198419 1107 40-41 -0.09413839079646635 1107 42-43 -0.005526199543666621 1107 44-45 -0.027803040337126106 1107 46-47 -0.03831195372118401 1107 48-49 0.12671149681483485 1107 50-51 0.12302306947673713 1107 52-53 0.045877677273999495 1107 54-55 0.24760634602196774 1107 56-57 0.22238855535935187 1107 58-59 0.03990528697628548 1107 60-61 0.18127967515601284 1107 62-63 0.27090611502766393 1107 64-65 0.267510996912371 1107 66-67 0.24161909581722796 1107 68-69 0.3020457605126339 1107 70-71 0.36233316722194076 1107 72-73 0.43606996884513194 1107 74-75 0.512672152384205 1107 76-77 0.42480724009196535 1107 78-79 0.3326203982295688 1107 80-81 0.3046612450642101 1107 82-83 0.20912876546881165 1107 84-85 0.2980041214268532 1107 86-87 0.4178932946766736 1107 88-89 0.5171945530678421 1107 90-91 0.6502849821710335 1107 92-93 0.7860551243445109 1107 94-95 0.7519128755383413 1107 96-97 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52-53 0.03448440725210844 2316 54-55 -0.04755883547547057 2316 56-57 -0.009879360801804182 2316 58-59 -0.05782442616990835 2316 60-61 0.008188431379082317 2316 62-63 0.13845202976639825 2316 64-65 -0.13357673266131798 2316 66-67 0.1151663493000541 2316 68-69 0.02928223013290676 2316 70-71 0.1358546219905108 2316 72-73 0.11120210009783449 2316 74-75 0.30122449588363054 2316 76-77 0.09355802451232975 2316 78-79 -0.031598179633739676 2316 80-81 0.0180598421719651 2316 82-83 0.10947112137950654 2316 84-85 0.1828456336498654 2316 86-87 -0.1866382146178367 2316 88-89 -0.13277755643026978 2316 90-91 -0.018255926222611407 2316 92-93 0.20949151308233027 2316 94-95 0.06991689177061744 2316 96-97 0.07651538612332587 2316 98-99 -0.02308283959044033 2316 100-101 -0.3988216018580637 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 19.0 5 39.0 6 114.0 7 241.0 8 604.0 9 1165.0 10 2025.0 11 3306.0 12 4944.0 13 7297.0 14 9344.0 15 13001.0 16 17348.0 17 24964.0 18 22501.0 19 13786.0 20 11845.0 21 11959.0 22 12472.0 23 13790.0 24 16051.0 25 19253.0 26 23296.0 27 29401.0 28 36866.0 29 46155.0 30 58031.0 31 74129.0 32 94818.0 33 124980.0 34 171661.0 35 245979.0 36 381869.0 37 601757.0 38 520415.0 39 49986.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.402767817921735 20.06341351695345 15.229193495702976 22.304625169421843 2 15.470383301405931 21.208461549428197 39.6639256372912 23.657229511874668 3 19.159105012264472 25.027256471958285 30.200095894758878 25.61354262101836 4 12.752277125348257 17.522291029337943 37.565383642815284 32.160048202498515 5 13.313521221982812 38.04052941183092 34.38681940576422 14.25912996042205 6 31.284389488793895 37.27038345147597 17.408312830013408 14.036914229716722 7 28.205477406579522 33.22074328192684 20.747859063217756 17.825920248275885 8 26.270924453988897 35.24657633950646 19.753795083855387 18.728704122649248 9 26.252953566076965 16.84251124212226 20.503394959597394 36.40114023220338 10-11 24.618179382952896 27.32803323152951 27.469549363787067 20.58423802173053 12-13 25.692199145947075 24.66036078980479 28.0247019885067 21.622738075741434 14-15 22.4160189268341 25.97018408342094 26.598771226469953 25.015025763275005 16-17 21.616314414753084 29.404874725278024 27.18033016910643 21.79848069086246 18-19 21.51529851558215 27.731668756392047 29.793499866062483 20.95953286196332 20-21 22.349143962944705 26.749178929265234 29.90588357115684 20.995793536633222 22-23 22.036277822955444 26.847896330854347 29.700035923279877 21.41578992291033 24-25 21.942376743148635 26.92716420183916 29.85921997809334 21.271239076918864 26-27 21.706428864468617 27.200539804686596 29.816330217300653 21.27670111354413 28-29 21.44809048136179 27.41279785783789 29.910861428982255 21.228250231818063 30-31 21.740614014354264 27.039592407922235 29.78746234162849 21.43233123609501 32-33 21.56159512452437 27.183950608946727 29.714112812155076 21.54034145437383 34-35 22.00298864495003 27.160370853095685 29.618780815945843 21.217859686008445 36-37 22.00241125090347 26.894714400705478 29.61732318884649 21.48555115954456 38-39 21.64466157213227 27.3420498306918 29.631907790390617 21.38138080678531 40-41 21.942297659975228 27.2072080183971 29.53025610780738 21.320238213820296 42-43 21.83067054470301 27.279745615024158 29.17571966022218 21.713864180050653 44-45 21.94124028364069 27.302789115914237 29.064916544875103 21.69105405556997 46-47 22.159886877199934 27.675354971933146 28.56018671715023 21.60457143371669 48-49 22.168553422309863 27.68130149747395 28.492486368012273 21.65765871220392 50-51 22.064179705877716 27.85035540338453 28.355753798085253 21.729711092652497 52-53 22.42254697380002 28.02556218114805 27.928691966975588 21.62319887807634 54-55 22.139890043677887 27.84000057026617 28.105399445191043 21.9147099408649 56-57 22.20608969236391 28.067019031132784 27.95795562578834 21.768935650714972 58-59 22.450159862115644 27.687623198127724 28.006972254299694 21.855244685456938 60-61 22.29067292158615 27.742642628446827 28.39345889694015 21.573225553026877 62-63 22.15176433600383 27.866619244532387 28.359768171794187 21.6218482476696 64-65 21.97706816726735 27.87499087855335 28.26166518128559 21.886275772893708 66-67 22.03232733878538 28.004627409843547 27.95591092142257 22.007134329948503 68-69 22.023491964874605 27.800438354602008 28.111439764510088 22.0646299160133 70-71 22.065399024994917 27.839906776487926 28.112152597224753 21.9825416012924 72-73 22.126214910809622 27.672222259739733 28.116598422313615 22.08496440713703 74-75 21.991902220361354 28.132824745950174 27.793009887364928 22.082263146323548 76-77 21.965508651162928 27.799650486864746 28.008135297149945 22.22670556482238 78-79 22.013568583136177 27.887085046945664 27.875041925818877 22.224304444099282 80-81 22.01580913484211 28.088020616630544 27.846220165594854 22.04995008293249 82-83 22.04065339266627 28.01091463943084 27.830585977172 22.117845990730885 84-85 22.015735293207293 27.90918243519429 27.74371067560163 22.33137159599679 86-87 21.862410530114932 27.846697446032938 27.903667786939558 22.38722423691258 88-89 21.97653879948466 27.844202531531593 27.58938359479287 22.58987507419088 90-91 21.962948024050956 27.803314979316557 27.67159754987946 22.562139446753026 92-93 22.011058637039397 27.68776937254663 27.757197728242673 22.5439742621713 94-95 21.76827909426725 27.62059816419314 27.83208437538221 22.779038366157405 96-97 21.75631100816306 27.63477978346393 27.887629050859488 22.72128015751352 98-99 21.683236609935737 27.798373876372512 27.463304542982648 23.055084970709103 100-101 21.509780507274996 27.749531212917983 27.699814924079476 23.040873355727552 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 316.0 1 243.0 2 211.5 3 264.0 4 259.5 5 267.5 6 479.5 7 1017.5 8 1722.5 9 2240.0 10 2319.0 11 2326.5 12 2518.0 13 2781.0 14 3133.5 15 3428.5 16 3591.0 17 3809.5 18 4048.0 19 4346.5 20 4800.5 21 5561.0 22 6630.5 23 7867.0 24 9582.0 25 12066.5 26 15356.0 27 19469.5 28 23431.0 29 27946.5 30 33732.0 31 39511.5 32 45619.5 33 51791.5 34 58276.5 35 63751.0 36 70070.5 37 77195.5 38 81298.5 39 83881.0 40 86082.5 41 88973.5 42 92263.0 43 96662.0 44 101161.0 45 103426.0 46 102412.0 47 103972.0 48 112113.0 49 111317.0 50 103215.5 51 96572.5 52 89048.0 53 83503.0 54 78499.0 55 70995.0 56 62614.5 57 55276.5 58 49285.0 59 43648.5 60 36621.5 61 29320.0 62 23209.5 63 17967.0 64 13373.5 65 10220.0 66 7633.0 67 5743.5 68 4076.5 69 2492.0 70 1755.0 71 1256.0 72 980.0 73 805.0 74 607.0 75 469.5 76 317.5 77 188.0 78 107.5 79 77.0 80 58.0 81 33.5 82 23.0 83 16.5 84 10.0 85 8.0 86 4.5 87 0.5 88 2.0 89 2.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07323418205447392 2 0.0 3 0.0 4 0.0 5 0.0018383580536215278 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.627626694759779E-5 12-13 0.004896035224441007 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.315914703187713E-4 24-25 8.441440042139669E-4 26-27 5.064864025283802E-4 28-29 0.002907607125625886 30-31 4.502101355807823E-4 32-33 0.0 34-35 0.0 36-37 1.8758755649199262E-5 38-39 6.190389364235758E-4 40-41 5.627626694759779E-5 42-43 0.012286984950225517 44-45 2.8138133473798894E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.8758755649199262E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.7517511298398524E-5 82-83 4.126926242823838E-4 84-85 4.689688912299816E-4 86-87 0.0 88-89 0.0 90-91 0.009266825290704436 92-93 0.0031702297047146756 94-95 0.0 96-97 0.0 98-99 1.3131128954439484E-4 100-101 0.011236494633870359 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2665422.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.31937380683577 #Duplication Level Percentage of deduplicated Percentage of total 1 74.41927785622168 39.6798929445066 2 14.12221038976202 15.059748295010023 3 4.897608713691969 7.834122892948744 4 2.243971069051987 4.78588528970031 5 1.1755882011015586 3.1340813368719815 6 0.7347434509445327 2.3505636427821623 7 0.4492064072003455 1.6765983039358632 8 0.3250044506532776 1.3863227034613932 9 0.2417520953039675 1.1601063304288224 >10 1.2378707266475557 12.010445616970035 >50 0.09947171469866149 3.651765878133151 >100 0.04998109107808281 4.765168771290219 >500 0.002397241359749496 0.8400366692507433 >1k 7.050709881499879E-4 0.6707887352537656 >5k 1.4101419762999756E-4 0.574435457864571 >10k+ 7.050709881499878E-5 0.4200371315916787 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11173 0.41918315373700676 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9166 0.3438855085611209 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6114 0.2293820640784086 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4624 0.1734809722437948 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.7517511298398524E-5 2 3.7517511298398524E-5 0.0 0.0 0.0 3.7517511298398524E-5 3 3.7517511298398524E-5 0.0 0.0 0.0 3.7517511298398524E-5 4 3.7517511298398524E-5 0.0 0.0 0.0 3.7517511298398524E-5 5 3.7517511298398524E-5 0.0 0.0 0.0 3.7517511298398524E-5 6 3.7517511298398524E-5 0.0 0.0 0.0 3.7517511298398524E-5 7 3.7517511298398524E-5 0.0 0.0 0.0 3.7517511298398524E-5 8 3.7517511298398524E-5 0.0 0.0 0.0 3.7517511298398524E-5 9 3.7517511298398524E-5 0.0 0.0 1.1255253389519558E-4 7.503502259679705E-5 10-11 3.7517511298398524E-5 0.0 0.0 1.3131128954439484E-4 1.1255253389519558E-4 12-13 3.7517511298398524E-5 0.0 0.0 1.6882880084279338E-4 1.500700451935941E-4 14-15 3.7517511298398524E-5 0.0 0.0 2.81381334737989E-4 1.500700451935941E-4 16-17 3.7517511298398524E-5 0.0 0.0 4.126926242823838E-4 1.500700451935941E-4 18-19 5.627626694759779E-5 0.0 0.0 4.502101355807823E-4 1.500700451935941E-4 20-21 1.1255253389519558E-4 0.0 0.0 4.8772764687918084E-4 1.500700451935941E-4 22-23 1.1255253389519558E-4 0.0 0.0 6.190389364235758E-4 1.500700451935941E-4 24-25 1.1255253389519558E-4 0.0 0.0 9.191790268107639E-4 1.500700451935941E-4 26-27 1.1255253389519558E-4 0.0 0.0 0.0013318716510931478 1.500700451935941E-4 28-29 2.063463121411919E-4 0.0 0.0 0.002326085700500709 2.2510506779039116E-4 30-31 2.2510506779039116E-4 0.0 0.0 0.004877276468791809 2.2510506779039116E-4 32-33 2.4386382343959042E-4 0.0 0.0 0.009060478978563245 2.626225790887897E-4 34-35 2.626225790887897E-4 0.0 0.0 0.013918996691705854 3.001400903871882E-4 36-37 2.626225790887897E-4 0.0 0.0 0.019002619472638854 3.188988460363875E-4 38-39 3.001400903871882E-4 0.0 0.0 0.024330106077011443 3.751751129839853E-4 40-41 3.188988460363875E-4 0.0 0.0 0.02875717241022247 3.751751129839853E-4 42-43 3.3765760168558677E-4 0.0 0.0 0.03453486915017585 3.751751129839853E-4 44-45 3.751751129839853E-4 0.0 0.0 0.04121298616129078 3.751751129839853E-4 46-47 4.8772764687918084E-4 0.0 0.0 0.05173664808049157 3.751751129839853E-4 48-49 5.627626694759779E-4 0.0 0.0 0.06535550468181023 3.751751129839853E-4 50-51 5.627626694759779E-4 0.0 0.0 0.07798014723372135 3.751751129839853E-4 52-53 5.627626694759779E-4 0.0 0.0 0.09996540885458288 3.751751129839853E-4 54-55 5.627626694759779E-4 0.0 0.0 0.14168488141840202 3.9393386863318454E-4 56-57 5.627626694759779E-4 0.0 0.0 0.20818467019481343 4.126926242823838E-4 58-59 5.627626694759779E-4 0.0 0.0 0.2776108248524999 4.126926242823838E-4 60-61 5.627626694759779E-4 0.0 0.0 0.34671808066415 4.126926242823838E-4 62-63 5.627626694759779E-4 0.0 0.0 0.43163896748807506 4.126926242823838E-4 64-65 5.627626694759779E-4 0.0 0.0 0.5342681196448442 4.126926242823838E-4 66-67 5.627626694759779E-4 0.0 0.0 0.6850885150644064 4.126926242823838E-4 68-69 5.627626694759779E-4 0.0 0.0 0.8650787755184732 4.126926242823838E-4 70-71 5.627626694759779E-4 0.0 0.0 1.0339075763612666 4.126926242823838E-4 72-73 5.815214251251771E-4 0.0 0.0 1.2181373155920525 4.126926242823838E-4 74-75 6.002801807743764E-4 0.0 0.0 1.4190811061062751 4.126926242823838E-4 76-77 6.002801807743764E-4 0.0 0.0 1.6524025088710155 4.502101355807823E-4 78-79 6.002801807743764E-4 0.0 0.0 1.8699290393791301 4.502101355807823E-4 80-81 6.37797692072775E-4 0.0 0.0 2.106664535672025 4.502101355807823E-4 82-83 6.37797692072775E-4 0.0 0.0 2.3764342006631596 4.502101355807823E-4 84-85 6.37797692072775E-4 0.0 0.0 2.7235274564402934 4.502101355807823E-4 86-87 6.37797692072775E-4 0.0 0.0 3.120481484732999 4.502101355807823E-4 88-89 6.37797692072775E-4 0.0 0.0 3.5901819674332995 4.502101355807823E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16390 0.0 26.975994 1 GGTATCA 11695 0.0 25.77285 1 CAACGCA 19440 0.0 22.69916 5 ATCAACG 19400 0.0 22.67251 3 TCAACGC 19450 0.0 22.638649 4 GTACATG 11475 0.0 22.49679 1 TATCAAC 19790 0.0 22.321712 2 GAGTACT 10245 0.0 22.277773 12-13 AACGCAG 19880 0.0 22.220242 6 TACATGG 11590 0.0 21.639183 2 ACATGGG 11335 0.0 21.245981 3 GTACTTT 10945 0.0 20.787485 14-15 AGTACTT 11080 0.0 20.513159 12-13 AGAGTAC 16875 0.0 20.308737 10-11 ACGCAGA 22280 0.0 19.805733 7 CGCAGAG 22440 0.0 19.558678 8 ACCGTCG 155 9.6295483E-4 18.38696 8 GCAGAGT 23830 0.0 18.17863 9 TAGCCCT 1750 0.0 17.91415 4 TACTTTT 12760 0.0 17.86787 14-15 >>END_MODULE