SRR937406_2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR937406_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	54575
Sequences flagged as poor quality	0
Sequence length	101
%GC	35

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1481	2.7136967475950526	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1221	2.2372881355932206	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	798	1.462207970682547	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	665	1.2185066422354558	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	406	0.7439303710490152	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	365	0.668804397617957	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	356	0.652313330279432	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	282	0.5167201099404489	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	278	0.5093907466788823	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	253	0.46358222629409074	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	170	0.31149793861658265	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	166	0.304168575355016	No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT	165	0.3023362345396244	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	159	0.2913421896472744	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	145	0.2656894182317911	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	143	0.2620247366010078	No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA	124	0.22721026110856618	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	121	0.22171323866239118	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	115	0.21071919377004122	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	113	0.2070545121392579	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	110	0.2015574896930829	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	108	0.19789280806229959	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	107	0.19606046724690795	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105	0.1923957856161246	No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	102	0.1868987631699496	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	99	0.1814017407237746	No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG	99	0.1814017407237746	No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA	96	0.17590471827759963	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	94	0.1722400366468163	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	94	0.1722400366468163	No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT	93	0.17040769583142465	No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA	89	0.163078332569858	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	88	0.16124599175446633	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	86	0.157581310123683	No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG	85	0.15574896930829132	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	84	0.1539166284928997	No Hit
CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA	81	0.1484196060467247	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	80	0.14658726523133303	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	79	0.14475492441594137	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	72	0.13192853870819973	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	72	0.13192853870819973	No Hit
GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT	70	0.12826385707741642	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	63	0.11543747136967476	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	63	0.11543747136967476	No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA	56	0.10261108566193312	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	56	0.10261108566193312	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	55	0.10077874484654145	No Hit

Adapter Content

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTAA	20	0.002057037	71.30584	1
GTGCTGC	25	0.004980201	57.044666	1
ATGGGAG	45	9.5507444E-4	42.216507	5
TATGAGT	45	9.5507444E-4	42.216507	6
GTTAGTA	50	0.0015961626	38.029778	1
GAGTAAC	50	0.0016033961	37.994858	9
GTATGAG	50	0.0016033961	37.994858	5
TGAGTAA	55	0.00255989	34.54078	8
ATGAGTA	55	0.00255989	34.54078	7
TAGTATG	55	0.00255989	34.54078	3
AGAGAAC	35	2.0428165E-4	33.92398	10-11
GGTGAGA	60	0.003920867	31.662382	9
AGTATGA	65	0.0057997815	29.226814	4
TTAGTAT	65	0.0057997815	29.226814	2
GTACATG	525	0.0	28.0696	1
TACATGG	525	0.0	28.043823	2
CATGGGA	255	0.0	27.937395	4
TGGGAAG	190	3.7471182E-10	27.496279	6
ACATGGG	520	0.0	27.400139	3
ATGGGAA	210	4.0017767E-11	27.139183	5

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content