##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937404_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 78043 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95720308035314 31.0 31.0 34.0 30.0 34.0 2 32.1081583229758 33.0 31.0 34.0 30.0 34.0 3 32.070230513947436 33.0 31.0 34.0 30.0 34.0 4 35.82587804159245 37.0 35.0 37.0 35.0 37.0 5 35.770818651256356 37.0 35.0 37.0 35.0 37.0 6 35.87073792652768 37.0 35.0 37.0 35.0 37.0 7 35.8074779288341 37.0 35.0 37.0 35.0 37.0 8 35.821252386504874 37.0 35.0 37.0 35.0 37.0 9 37.404584652050794 39.0 37.0 39.0 34.0 39.0 10-11 37.35492613046654 39.0 37.0 39.0 34.0 39.0 12-13 37.26446958727881 39.0 37.0 39.0 33.5 39.0 14-15 38.18246351370398 40.0 37.0 41.0 33.0 41.0 16-17 38.223799700165294 40.0 37.5 41.0 33.5 41.0 18-19 38.39008623451174 40.0 38.0 41.0 34.0 41.0 20-21 38.427507912304755 40.0 38.0 41.0 34.0 41.0 22-23 38.27885268377689 40.0 37.5 41.0 34.0 41.0 24-25 38.122720807759826 40.0 37.0 41.0 33.0 41.0 26-27 37.856040900529194 40.0 36.5 41.0 33.0 41.0 28-29 37.61426393142242 39.5 36.0 41.0 33.0 41.0 30-31 37.28970567507656 39.0 35.5 41.0 33.0 41.0 32-33 36.81234063272811 39.0 35.0 41.0 33.0 41.0 34-35 36.436413259357025 38.0 35.0 41.0 31.5 41.0 36-37 36.023685660469226 38.0 35.0 41.0 31.0 41.0 38-39 35.64015991184347 38.0 35.0 40.0 30.0 41.0 40-41 35.15355637276886 38.0 35.0 40.0 29.0 41.0 42-43 34.509770254859504 38.0 35.0 40.0 24.0 41.0 44-45 33.600297272016704 38.0 33.5 40.0 10.0 41.0 46-47 32.458952116141106 38.0 33.0 40.0 2.0 41.0 48-49 31.450572120497675 38.0 32.5 40.0 2.0 41.0 50-51 30.806311904975463 37.0 31.0 40.0 2.0 41.0 52-53 30.366624809399944 37.0 30.0 40.0 2.0 41.0 54-55 29.968985046705022 37.0 28.5 40.0 2.0 41.0 56-57 29.527580948964033 36.0 27.0 40.0 2.0 41.0 58-59 29.123438360903606 36.0 26.0 40.0 2.0 41.0 60-61 28.63605960816473 35.0 23.5 39.5 2.0 41.0 62-63 28.171873198108734 35.0 21.0 39.0 2.0 41.0 64-65 27.68133593019233 35.0 16.0 39.0 2.0 40.0 66-67 27.147047140678858 35.0 7.5 38.5 2.0 40.0 68-69 26.60571095421755 35.0 2.0 37.5 2.0 40.0 70-71 26.08634342605999 34.0 2.0 37.0 2.0 39.5 72-73 25.56342657253053 34.0 2.0 36.5 2.0 39.0 74-75 25.09463372756045 34.0 2.0 36.0 2.0 39.0 76-77 24.010782517330192 31.5 2.0 35.0 2.0 37.0 78-79 24.34882692874441 33.0 2.0 35.0 2.0 37.0 80-81 24.08048767986879 33.0 2.0 35.0 2.0 37.0 82-83 23.743333803159796 33.0 2.0 35.0 2.0 36.0 84-85 23.398466230155172 32.5 2.0 35.0 2.0 36.0 86-87 23.029201850262034 32.0 2.0 35.0 2.0 35.5 88-89 22.58224312238125 32.0 2.0 35.0 2.0 35.0 90-91 22.104320695001473 31.0 2.0 35.0 2.0 35.0 92-93 21.840280358264035 31.0 2.0 34.0 2.0 35.0 94-95 21.481324398088233 31.0 2.0 34.0 2.0 35.0 96-97 21.078584882692873 31.0 2.0 34.0 2.0 35.0 98-99 20.491581564009586 30.0 2.0 34.0 2.0 35.0 100-101 18.798425227118386 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.03327396866695764 1101 2 0.19429508149713115 1101 3 0.2878446538538739 1101 4 0.34065876123287353 1101 5 -0.2398865326411581 1101 6 0.07594056128714044 1101 7 -0.16436800911115057 1101 8 0.15926590770022386 1101 9 -0.03152639197958251 1101 10-11 0.008055707870873619 1101 12-13 -0.1530631707306611 1101 14-15 0.030717034021449763 1101 16-17 0.02764547470752632 1101 18-19 0.1548424489765452 1101 20-21 0.2873038151130487 1101 22-23 0.4499703142274427 1101 24-25 0.11894525207815576 1101 26-27 -0.2622014523382745 1101 28-29 -0.34267056575897925 1101 30-31 0.13386255221369936 1101 32-33 0.44947069633783343 1101 34-35 0.492472830321141 1101 36-37 0.6414972656900559 1101 38-39 0.743599801303489 1101 40-41 1.3490364653938443 1101 42-43 1.3144494214323075 1101 44-45 1.6046809008789253 1101 46-47 1.2466649060925334 1101 48-49 1.2491543220197414 1101 50-51 1.2674770744230308 1101 52-53 1.261148542943456 1101 54-55 1.484177315226546 1101 56-57 1.2064288764977498 1101 58-59 1.1940348522755535 1101 60-61 1.2826529122467285 1101 62-63 1.5115018271834906 1101 64-65 1.266231277389327 1101 66-67 1.5989263966558056 1101 68-69 1.9490407240606658 1101 70-71 2.1309985150150226 1101 72-73 1.8327990565407752 1101 74-75 1.2713914534421562 1101 76-77 1.3885327890571517 1101 78-79 1.676595072664714 1101 80-81 1.8038456141478498 1101 82-83 1.8557895569035168 1101 84-85 1.929805538850136 1101 86-87 1.8926867100371183 1101 88-89 1.9561049371694246 1101 90-91 2.470797129876207 1101 92-93 1.9522479332381657 1101 94-95 1.923383116274838 1101 96-97 1.9003828060588468 1101 98-99 2.005435968594867 1101 100-101 1.7307300338697402 1102 1 -0.3637884154604727 1102 2 -0.3497500206875088 1102 3 -0.10591855967677688 1102 4 0.019834236919976433 1102 5 -0.10757504567850873 1102 6 -0.23413695809270507 1102 7 -0.512324316369785 1102 8 0.02290227133659073 1102 9 -0.2528089853552018 1102 10-11 -0.2547173717626663 1102 12-13 -0.35752053507175674 1102 14-15 -0.6255549885296503 1102 16-17 -0.11426784453137628 1102 18-19 -0.19848736074862217 1102 20-21 -0.4145989333013276 1102 22-23 -0.07143560543429572 1102 24-25 -0.2672422038767408 1102 26-27 -0.05959398228894486 1102 28-29 -0.03770227823255823 1102 30-31 -0.1010740228391569 1102 32-33 0.1109400409467085 1102 34-35 0.183011942371877 1102 36-37 0.30305470050330285 1102 38-39 0.6610592798094785 1102 40-41 0.5742831179660826 1102 42-43 1.1762358907769155 1102 44-45 1.251879632379982 1102 46-47 0.6433350963673803 1102 48-49 1.125563765289641 1102 50-51 2.212332606487866 1102 52-53 1.9693056958680515 1102 54-55 1.5512139607515678 1102 56-57 1.759106818710574 1102 58-59 2.402896726835099 1102 60-61 1.8917966754602595 1102 62-63 2.0099162035048437 1102 64-65 2.4134300088903835 1102 66-67 3.1829644516240947 1102 68-69 2.091218313912673 1102 70-71 2.666851228193316 1102 72-73 2.7137891904378897 1102 74-75 2.5513421229277107 1102 76-77 2.6470775388809713 1102 78-79 2.511073578654848 1102 80-81 2.3205827529780123 1102 82-83 2.6916775061635576 1102 84-85 2.995432741105244 1102 86-87 3.160040480297866 1102 88-89 2.7621303071482792 1102 90-91 2.767925389213765 1102 92-93 2.853058363118368 1102 94-95 2.3165296983749037 1102 96-97 2.1123278377713213 1102 98-99 1.7149144745850045 1102 100-101 1.965226157900748 1103 1 -0.029613331285389677 1103 2 -0.1973931183306057 1103 3 -0.046996000754219835 1103 4 -0.20154623446049413 1103 5 0.22070778260432178 1103 6 0.1640111900554473 1103 7 -0.14111219515766749 1103 8 -0.22036372192940235 1103 9 -0.10129383384004598 1103 10-11 -0.3595153515606455 1103 12-13 -0.06122423877545202 1103 14-15 -0.5119186248932763 1103 16-17 -0.6517257569892934 1103 18-19 0.011108598847343387 1103 20-21 -0.09641711511951456 1103 22-23 0.27199873800005037 1103 24-25 0.284104597470062 1103 26-27 0.19419389649893048 1103 28-29 -0.29906591459618426 1103 30-31 -0.38390230566744066 1103 32-33 -0.033841103834433284 1103 34-35 0.29286042722036143 1103 36-37 0.04632069376929593 1103 38-39 0.060890929641125524 1103 40-41 0.5006299193128783 1103 42-43 0.7700911096321406 1103 44-45 0.2295732350735875 1103 46-47 0.31589401892630065 1103 48-49 -0.642533877807999 1103 50-51 -0.9783239591687014 1103 52-53 -0.978084876522523 1103 54-55 -1.381025096487491 1103 56-57 -1.128519443915689 1103 58-59 -1.1438204448820706 1103 60-61 -0.5168733582097715 1103 62-63 0.29358623717487475 1103 64-65 0.08093842639880222 1103 66-67 0.45400822266786633 1103 68-69 0.6168917145860746 1103 70-71 0.958517894859984 1103 72-73 1.2074760591247546 1103 74-75 1.2003320219176068 1103 76-77 1.1395017813052135 1103 78-79 0.7038345214157893 1103 80-81 0.17916861156387043 1103 82-83 -0.46699252750647346 1103 84-85 -0.10852348785098442 1103 86-87 -0.6307844355270511 1103 88-89 -0.4213713763534024 1103 90-91 -0.427781681493304 1103 92-93 -0.32413018907018554 1103 94-95 -0.22261171576650796 1103 96-97 0.11485309029657387 1103 98-99 0.4447124543829837 1103 100-101 0.7800409727155646 1104 1 0.18976694007507788 1104 2 0.10119507382194115 1104 3 0.041471179297118965 1104 4 -0.07265001150486938 1104 5 0.3672974415702157 1104 6 0.13880033563666672 1104 7 0.11301321738358894 1104 8 0.08995897700715716 1104 9 0.1396302585691913 1104 10-11 -0.10016441646713048 1104 12-13 -0.05347763078132317 1104 14-15 0.01943451042024691 1104 16-17 0.1398950717602503 1104 18-19 -0.08968898091062982 1104 20-21 -0.0584633197399711 1104 22-23 -0.47868883075682334 1104 24-25 0.1943539557392242 1104 26-27 0.07331930904018691 1104 28-29 0.44392088408368124 1104 30-31 0.5441780023633669 1104 32-33 0.8131977667192913 1104 34-35 0.6055666978474292 1104 36-37 1.103159711004352 1104 38-39 1.4005957334548427 1104 40-41 1.6476779574500284 1104 42-43 1.5152322904168827 1104 44-45 2.1342303674534975 1104 46-47 1.6836516280205416 1104 48-49 1.7996186707801982 1104 50-51 1.6001963928665042 1104 52-53 2.165937859084373 1104 54-55 1.8133276721227034 1104 56-57 2.068812789307632 1104 58-59 1.5951784550079147 1104 60-61 2.2532953253252472 1104 62-63 3.004440655950088 1104 64-65 1.9426454304325382 1104 66-67 1.5061717723561685 1104 68-69 2.100894281509433 1104 70-71 2.673864429513447 1104 72-73 2.4214474562644703 1104 74-75 2.5677687655919748 1104 76-77 2.794204751602244 1104 78-79 2.730282999596941 1104 80-81 2.672292923995112 1104 82-83 2.403836222035146 1104 84-85 2.6292836261937502 1104 86-87 2.968133789628798 1104 88-89 3.0383829324108085 1104 90-91 2.8414702436992165 1104 92-93 3.5020982671087637 1104 94-95 3.777938339238993 1104 96-97 3.749066511638709 1104 98-99 3.6993879219297376 1104 100-101 4.348889524237382 1105 1 -0.119769276704492 1105 2 -0.098075379539182 1105 3 0.057000100610302695 1105 4 0.06648495462332704 1105 5 -0.16204075540737506 1105 6 -0.17448783528568867 1105 7 -0.16567359866643727 1105 8 -0.30389198703662146 1105 9 -0.4451534829628585 1105 10-11 -0.5872931293384269 1105 12-13 -0.19480123682613737 1105 14-15 -0.010164238928368263 1105 16-17 -0.2112774139093716 1105 18-19 -0.3548270736672734 1105 20-21 -0.6192241326633692 1105 22-23 -0.19364453685374627 1105 24-25 -0.5382150906391061 1105 26-27 0.19463249299015928 1105 28-29 0.0776884713687167 1105 30-31 0.46507430251968884 1105 32-33 -0.22833428122234523 1105 34-35 0.104263935992293 1105 36-37 0.17809457291159703 1105 38-39 0.31357124153196025 1105 40-41 0.6037488276949503 1105 42-43 0.8225277568056342 1105 44-45 1.2059864904342135 1105 46-47 0.8931357343259094 1105 48-49 1.2620470188781638 1105 50-51 1.4586643449248662 1105 52-53 0.8709404646080827 1105 54-55 1.7478248060466228 1105 56-57 1.8611004391252486 1105 58-59 1.3032555785097415 1105 60-61 1.3376100725894489 1105 62-63 1.3505063151475873 1105 64-65 1.571045638874434 1105 66-67 0.9878288854358956 1105 68-69 1.1101178354437735 1105 70-71 0.7979915790705085 1105 72-73 0.8156631936276817 1105 74-75 2.134834945894216 1105 76-77 2.1592386234104772 1105 78-79 1.530491441493762 1105 80-81 1.327999020920597 1105 82-83 0.9839821313629216 1105 84-85 0.8542445433380976 1105 86-87 0.4246736541415679 1105 88-89 0.8405194618532228 1105 90-91 1.1444405407289189 1105 92-93 1.5282577251598326 1105 94-95 1.4077976409586377 1105 96-97 1.62947297333751 1105 98-99 1.2558235654940937 1105 100-101 1.6994366842165398 1106 1 0.2679722289583957 1106 2 0.35335699887520633 1106 3 -0.3921671212417941 1106 4 0.11045564083251946 1106 5 0.4510172059517217 1106 6 0.18780397017734174 1106 7 0.19222113817566822 1106 8 -0.04326573786939747 1106 9 -0.0042474203379399 1106 10-11 0.0430866174970177 1106 12-13 0.006930941721492445 1106 14-15 0.2391362274273945 1106 16-17 -0.014008926234481578 1106 18-19 -0.18913753336081385 1106 20-21 0.5259457540787977 1106 22-23 0.06085246514491871 1106 24-25 -0.6623323458023194 1106 26-27 -0.5500044157373551 1106 28-29 -0.381871821769181 1106 30-31 0.13209628786280092 1106 32-33 0.1659362051200972 1106 34-35 0.22851443565917862 1106 36-37 0.5706995029582274 1106 38-39 -0.5232042414793625 1106 40-41 -0.12196736151224741 1106 42-43 -0.41884466158211353 1106 44-45 -0.024060145142072997 1106 46-47 0.638971843586404 1106 48-49 -0.2879862922477514 1106 50-51 -0.8508626370871255 1106 52-53 -1.0651687958849223 1106 54-55 -0.418530956778163 1106 56-57 -0.24156445094803658 1106 58-59 0.09492779703072785 1106 60-61 0.3254848227560032 1106 62-63 0.3615656089517181 1106 64-65 0.5003594296805645 1106 66-67 -0.08525572482393073 1106 68-69 -0.38375291738767103 1106 70-71 0.1832014391637813 1106 72-73 0.5351835134144913 1106 74-75 0.6736090964605062 1106 76-77 0.6062739332039477 1106 78-79 0.830358196990101 1106 80-81 0.8018830982024063 1106 82-83 0.3036356956533943 1106 84-85 0.19082015959392606 1106 86-87 0.2132146268264421 1106 88-89 0.6828245917666145 1106 90-91 0.708997502754233 1106 92-93 1.1279912361525035 1106 94-95 1.3463053025175995 1106 96-97 0.9559220073148573 1106 98-99 0.16974761612700462 1106 100-101 0.8228210946096084 1107 1 -0.2425762942483516 1107 2 -0.37035608129356845 1107 3 0.0555965918383734 1107 4 0.29104635813209967 1107 5 -0.6981811062845651 1107 6 -0.3541369580927025 1107 7 -0.02111219515766294 1107 8 -0.34740075896644385 1107 9 -0.8746271671733794 1107 10-11 -0.3072931293384258 1107 12-13 -0.5558538684050802 1107 14-15 -0.9052519582266143 1107 16-17 -0.2765405718041052 1107 18-19 -0.8450025122637683 1107 20-21 -0.3630837817861803 1107 22-23 -0.46522348422217163 1107 24-25 -0.07996947660401332 1107 26-27 -0.5608061035010721 1107 28-29 -1.7640659145961877 1107 30-31 -1.043346750111887 1107 32-33 -0.6125448075381357 1107 34-35 -1.0304729061129692 1107 36-37 -0.9966422691936643 1107 38-39 -1.2748498110996138 1107 40-41 -0.9134441547611871 1107 42-43 -0.6602792607382284 1107 44-45 0.2747584202587774 1107 46-47 -1.199846721814442 1107 48-49 -1.4688301741042906 1107 50-51 -1.19221284805759 1107 52-53 -0.8466033950410399 1107 54-55 -1.0862102816726775 1107 56-57 -1.8690749994712412 1107 58-59 -2.9227093337709604 1107 60-61 -2.4950215063579186 1107 62-63 -1.8982656146769763 1107 64-65 -1.4103578698974921 1107 66-67 -1.9500658514062081 1107 68-69 -0.6684786557842983 1107 70-71 -1.118148771806684 1107 72-73 -1.1703017186530218 1107 74-75 -1.2307790891935007 1107 76-77 -0.8621648853614516 1107 78-79 -0.5130173304360603 1107 80-81 -0.413053610658352 1107 82-83 0.28819265767871016 1107 84-85 0.8440691047416067 1107 86-87 0.4362526015099881 1107 88-89 0.1278878829058563 1107 90-91 -0.5088927926044171 1107 92-93 -0.9720931520331497 1107 94-95 -0.8715006046553988 1107 96-97 -0.9140357985923124 1107 98-99 -1.2575097678392382 1107 100-101 -1.9314405087659168 1108 1 -0.45718816639447013 1108 2 -0.11811863837119319 1108 3 -0.31170934423468566 1108 4 -0.2139764729181337 1108 5 0.04300610832730456 1108 6 0.11289500537761654 1108 7 -0.21873776593392336 1108 8 0.022279606330357637 1108 9 0.3187974903608648 1108 10-11 0.06476166518212523 1108 12-13 0.18414613159491466 1108 14-15 0.013195530357855034 1108 16-17 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2310 100-101 0.683976157900748 2311 1 0.2976535908091158 2311 2 -0.07702274796023545 2311 3 0.41053912057400765 2311 4 -0.4997582395690543 2311 5 0.4553821121062356 2311 6 0.2371274097233922 2311 7 0.12325562093428744 2311 8 0.4006452180450495 2311 9 0.08261421213695996 2311 10-11 0.664431008592608 2311 12-13 0.4772495798707723 2311 14-15 0.42486298430212344 2311 16-17 0.45587322129934194 2311 18-19 0.6945377176212943 2311 20-21 0.9840426549954273 2311 22-23 0.5713282399157578 2311 24-25 -0.12042924671895605 2311 26-27 0.1525272298322662 2311 28-29 0.3058191428750803 2311 30-31 0.7966532498881165 2311 32-33 0.5081448476342771 2311 34-35 0.8129753697490969 2311 36-37 0.23853014459943722 2311 38-39 0.2382536371762427 2311 40-41 -0.02746714326693933 2311 42-43 -0.9393597205083424 2311 44-45 -0.21742548778721016 2311 46-47 -3.08145591721674 2311 48-49 -3.6773359212307284 2311 50-51 -3.7393392848392004 2311 52-53 -4.223614889293913 2311 54-55 -4.778853959833597 2311 56-57 -5.424017528206878 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16-17 0.8641045894862174 2312 18-19 0.9309114662308531 2312 20-21 -0.04265367425929156 2312 22-23 0.43284103190686096 2312 24-25 0.4869122438260973 2312 26-27 0.7170433588645224 2312 28-29 0.5382996768016568 2312 30-31 0.3289113144042446 2312 32-33 0.25842293439735187 2312 34-35 -1.3388600028871593 2312 36-37 -1.6101906562904347 2312 38-39 -1.8451723917447822 2312 40-41 -3.4874226493848504 2312 42-43 -2.0088814112758655 2312 44-45 -2.5906179238272458 2312 46-47 -4.923502635792936 2312 48-49 -6.296357055824721 2312 50-51 -6.986836503971567 2312 52-53 -6.622947481062546 2312 54-55 -7.076962969844722 2312 56-57 -7.233806182266942 2312 58-59 -7.3848598714053715 2312 60-61 -5.8343763450676 2312 62-63 -5.585147335107084 2312 64-65 -4.7946589451663115 2312 66-67 -5.144259399793306 2312 68-69 -5.908048548257415 2312 70-71 -5.065675653527112 2312 72-73 -4.398258707900332 2312 74-75 -4.534435003171996 2312 76-77 -4.058078863856075 2312 78-79 -4.912802276672618 2312 80-81 -4.567247159045447 2312 82-83 -3.515678310063226 2312 84-85 -3.247113690957317 2312 86-87 -3.327618366231949 2312 88-89 -3.266950826771563 2312 90-91 -3.1491078463678583 2312 92-93 -2.987361969237451 2312 94-95 -2.4932210347629216 2312 96-97 -2.494465906119199 2312 98-99 -2.2828861119252615 2312 100-101 -2.655741584034736 2313 1 -0.08571354915031293 2313 2 -0.4887874538425869 2313 3 -0.2730308591420183 2313 4 -0.33444383794633836 2313 5 0.39554438391151336 2313 6 -0.5478624482887824 2313 7 -0.7489553324125637 2313 8 -0.6348517393586022 2313 9 0.2712551857677923 2313 10-11 -0.22552842345607615 2313 12-13 -0.44820680958155634 2313 14-15 -0.3844676445011217 2313 16-17 0.018753545842955077 2313 18-19 0.28480140930485476 2313 20-21 -0.3709269190410751 2313 22-23 0.10124710401311887 2313 24-25 -0.14781261385891042 2313 26-27 -0.4798257113442048 2313 28-29 -0.061320816556971636 2313 30-31 -0.9827585148177675 2313 32-33 -1.2448977487146067 2313 34-35 -0.37870820023061924 2313 36-37 -0.15193638684072397 2313 38-39 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-0.058335845597106584 2315 20-21 -0.20247772118011653 2315 22-23 -0.17370833270702235 2315 24-25 -0.2672422038767408 2315 26-27 -1.165654588349554 2315 28-29 -1.1816416721719492 2315 30-31 -0.9306194773846101 2315 32-33 -1.415575110568433 2315 34-35 -1.835927451567514 2315 36-37 -2.3560362085876037 2315 38-39 -1.8275770838268883 2315 40-41 -4.967383548700589 2315 42-43 -5.115430775889749 2315 44-45 -4.29736279186244 2315 46-47 -2.996816418784139 2315 48-49 -2.400951386225504 2315 50-51 -6.340697696542438 2315 52-53 -6.110239758677402 2315 54-55 -7.293483008945405 2315 56-57 -7.627256817653063 2315 58-59 -7.949376000437628 2315 60-61 -7.611991203327616 2315 62-63 -8.346144402555762 2315 64-65 -9.25702453656416 2315 66-67 -8.279156760497116 2315 68-69 -9.098175625481268 2315 70-71 -7.95814877180668 2315 72-73 -8.327877476228782 2315 74-75 -8.213809392223805 2315 76-77 -7.796104279300847 2315 78-79 -9.454835512254242 2315 80-81 -9.338508156112896 2315 82-83 -9.592413402927352 2315 84-85 -9.440173319500818 2315 86-87 -8.874050428793044 2315 88-89 -8.699990904972932 2315 90-91 -8.894953398665022 2315 92-93 -8.290881030821028 2315 94-95 -8.247864241019032 2315 96-97 -7.592217616774132 2315 98-99 -7.019934010263482 2315 100-101 -6.815076872402283 2316 1 -1.8566113819676495 2316 2 -0.9980753795391841 2316 3 -1.7482630572844329 2316 4 -0.933515045376673 2316 5 0.03795924459262778 2316 6 -0.42711941423305433 2316 7 -0.07093675656117426 2316 8 -0.34073409229977614 2316 9 -0.013574535594436554 2316 10-11 0.3890226601352609 2316 12-13 0.19730402633175714 2316 14-15 -0.04700634419152294 2316 16-17 -0.2165405718041029 2316 18-19 -0.24693233682517501 2316 20-21 -0.6139609747686379 2316 22-23 -0.46996032632743123 2316 24-25 -0.193478248533836 2316 26-27 -0.32115698069404885 2316 28-29 -0.6038904759996981 2316 30-31 -0.30860990800662336 2316 32-33 -1.1651763864855056 2316 34-35 -1.145736064007707 2316 36-37 -1.0745370060357757 2316 38-39 -0.08116560057329991 2316 40-41 0.4090119855896788 2316 42-43 0.2067382831214175 2316 44-45 0.6296707009605313 2316 46-47 0.20892520801011472 2316 48-49 -0.5747950863849951 2316 50-51 -0.6676514445488202 2316 52-53 0.020940464608084852 2316 54-55 -0.9916488781639075 2316 56-57 -1.7388995608747528 2316 58-59 -1.6862181057007852 2316 60-61 -1.3887057168842354 2316 62-63 -2.0889673690629387 2316 64-65 -2.4236912032308275 2316 66-67 -2.5148026935114736 2316 68-69 -1.6109347961351759 2316 70-71 -3.7783242104031736 2316 72-73 -6.052757859003901 2316 74-75 -5.691480843579466 2316 76-77 -5.946024534484259 2316 78-79 -5.658982242716764 2316 80-81 -5.398316768553087 2316 82-83 -4.887070500216026 2316 84-85 -5.103650193504009 2316 86-87 -5.370063187963698 2316 88-89 -4.741059485515198 2316 90-91 -5.152927880323713 2316 92-93 -4.363847537998062 2316 94-95 -4.313254990620308 2316 96-97 -5.225790184557226 2316 98-99 -4.536281697663799 2316 100-101 -5.811089631572937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 5.0 6 9.0 7 27.0 8 73.0 9 125.0 10 221.0 11 415.0 12 703.0 13 951.0 14 1143.0 15 1771.0 16 2536.0 17 3321.0 18 2834.0 19 1536.0 20 1231.0 21 1172.0 22 1085.0 23 1041.0 24 1071.0 25 1135.0 26 1103.0 27 1205.0 28 1273.0 29 1540.0 30 1760.0 31 2038.0 32 2370.0 33 2992.0 34 4004.0 35 5887.0 36 7856.0 37 12462.0 38 10158.0 39 990.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.79845398489879 22.50054482290051 16.316484418065045 15.384516774135653 2 16.106505388055304 25.975423804825542 37.498558486987946 20.419512320131208 3 20.7078149225427 29.267198851914973 33.70449623925272 16.32048998628961 4 11.507758543367117 21.52787565829094 37.2589469907615 29.705418807580436 5 9.168492676738554 42.9400684273248 33.58064557464857 14.310793321288074 6 23.809950924490344 41.3182476327153 17.044449854567354 17.827351588227003 7 21.352331407044833 41.91279166613277 19.95566546647361 16.77921146034878 8 25.997206668118856 33.01128864856553 19.566136616993195 21.425368066322413 9 25.731968273900286 18.338608203170047 22.280025114360033 33.64939840856964 10-11 23.382622400471533 29.656727701395386 25.73901567084812 21.22163422728496 12-13 26.95481688408211 30.13211512340142 26.6440708372844 16.268997155232068 14-15 22.39150212062581 29.882244403726148 31.04506490011916 16.681188575528875 16-17 16.101380008456875 33.20349038350653 31.228937893212716 19.46619171482388 18-19 15.059646605076688 30.971387568391783 37.85028766192996 16.118678164601565 20-21 17.514062760273184 28.195994515843832 38.26224004715349 16.027702676729493 22-23 16.79480279849312 28.62561185002947 38.16598754516799 16.413597806309426 24-25 17.50381200107633 29.520264469587275 38.27377215124995 14.70215137808644 26-27 16.73628640621196 28.787335187012285 40.03049600861064 14.445882398165114 28-29 15.102414771817196 31.781574951467505 38.581890172410475 14.534120104304815 30-31 16.00399779608677 30.269210563407352 38.50505490562895 15.221736734876925 32-33 15.319118947247032 30.94319798060044 38.8234691131812 14.914213958971336 34-35 16.116756147252158 30.75612162525787 37.98995425598709 15.13716797150289 36-37 16.364055712876237 30.563279217867073 37.86374178337583 15.20892328588086 38-39 15.701600399779608 31.761977371449074 37.607472803454506 14.928949425316812 40-41 15.415219814717526 31.984931383980626 37.010366080237816 15.58948272106403 42-43 15.190562846011893 32.139378716424034 36.11659319253639 16.55346524502768 44-45 16.3960893353664 31.67997129787425 34.98840382865856 16.93553553810079 46-47 17.39361633971016 31.908691362454032 34.05814743154415 16.639544866291658 48-49 17.3430032161757 31.537742014017912 33.93834168343093 17.18091308637546 50-51 16.715784887818256 32.23607498430353 33.5840498186897 17.464090309188524 52-53 17.59286547159899 33.18106684776341 31.540304703817128 17.68576297682047 54-55 17.019463629025026 31.75941468164986 31.90997270735364 19.311148981971478 56-57 17.65629204412952 32.73579949515011 31.052752969516806 18.555155491203568 58-59 18.82423792012096 31.82924797867842 30.88361544276873 18.46289865843189 60-61 17.184116448624476 31.093115333854414 32.97925502607537 18.74351319144574 62-63 16.771523390951142 30.825314249836627 32.83830708711864 19.564855272093588 64-65 16.09241059415963 31.547352100764964 32.53847238061069 19.82176492446472 66-67 16.642107556090874 30.574170649513732 31.58002639570493 21.203695398690463 68-69 16.245531309662624 30.088540932562818 32.065656112655844 21.600271645118717 70-71 16.97781991978781 30.706789846623018 31.655625744781723 20.659764488807454 72-73 16.476814064041616 29.454275207257535 32.9273605576413 21.141550171059546 74-75 15.408172417769691 31.0899119716054 31.385902643414525 22.116012967210384 76-77 15.804748664197943 29.42352292966698 32.19571261996592 22.57601578616916 78-79 15.419063849416348 30.30508822059634 31.136681060441042 23.139166869546276 80-81 15.604858859859307 29.02053995874069 31.505708391527747 23.868892789872252 82-83 15.019284240739081 28.728393321630385 31.721615007111463 24.530707430519072 84-85 14.68293120459234 28.985302974001513 30.861191907025614 25.470573914380534 86-87 14.68293120459234 29.082685186371616 30.401189088066833 25.83319452096921 88-89 14.788001486360084 28.734800046128417 29.55934548902528 26.917852978486216 90-91 14.79288803459875 27.63222809546692 30.341182123978854 27.233701745955468 92-93 14.202332137365453 27.484623270117886 30.444003075345975 27.869041517170682 94-95 13.896185436233871 27.59440308547852 30.203861973527413 28.305549504760197 96-97 13.592506695027101 27.78788616531912 30.075086811116947 28.544520328536834 98-99 14.213958971336316 27.85515677254847 28.525300155042732 29.405584101072485 100-101 13.657130041969692 26.577387626950312 28.875788934097972 30.889693396982025 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 562.0 1 348.5 2 142.0 3 160.5 4 144.5 5 140.0 6 206.0 7 304.0 8 379.0 9 398.5 10 350.0 11 292.5 12 293.5 13 300.5 14 301.0 15 321.0 16 355.5 17 401.0 18 440.5 19 484.5 20 554.0 21 588.5 22 645.0 23 738.5 24 834.0 25 960.0 26 1157.0 27 1591.0 28 1853.0 29 1946.0 30 2128.5 31 2285.0 32 2470.0 33 2551.5 34 2656.5 35 2836.0 36 3021.5 37 3083.0 38 2737.0 39 2343.5 40 2182.5 41 2068.5 42 1943.0 43 1824.0 44 1780.0 45 1777.5 46 1844.0 47 2520.5 48 3913.0 49 3724.5 50 2486.0 51 1789.5 52 1218.0 53 980.0 54 823.0 55 701.5 56 623.5 57 565.0 58 471.0 59 383.0 60 330.5 61 295.0 62 235.0 63 154.5 64 93.5 65 60.0 66 47.5 67 44.5 68 36.0 69 24.0 70 16.0 71 13.5 72 10.0 73 7.0 74 10.0 75 8.0 76 4.5 77 5.0 78 3.5 79 0.5 80 0.5 81 1.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046128416385838575 2 0.0 3 0.0 4 0.0 5 0.005125379598426508 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0064067244980331355 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001281344899606627 24-25 0.0 26-27 0.0 28-29 0.0032033622490165677 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014094793895672898 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.007047396947836449 92-93 0.0038440346988198814 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.013454121445869585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 78043.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.55215714413849 #Duplication Level Percentage of deduplicated Percentage of total 1 86.57074883879008 48.95762592417 2 7.467995921604169 8.446625578206886 3 2.21366262603376 3.755621900747024 4 0.9312337147388694 2.106531014953295 5 0.6230882519542313 1.7618492369591126 6 0.36932140024923527 1.2531553118152814 7 0.2854877081681205 1.1301462014530452 8 0.199388240625354 0.9020668093230655 9 0.15407273139231903 0.7841830785592557 >10 0.9878781012801631 10.729982189305895 >50 0.10649144669763226 3.9952333969734632 >100 0.07930214115781126 8.33130453724229 >500 0.004531550923303501 2.4627448970439376 >1k 0.006797326384955251 5.38292992324744 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1567 2.007867457683585 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1481 1.8976717963174148 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1153 1.4773906692464411 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 966 1.2377791730200018 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 956 1.2249657240239356 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 397 0.5086939251438309 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 376 0.48178568225209184 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 357 0.4574401291595659 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 347 0.4446266801634996 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 325 0.4164370923721538 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 272 0.3485258126930026 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 256 0.3280242942992965 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 243 0.3113668106044104 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 231 0.29599067180913086 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 213 0.2729264636162116 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 204 0.26139435951975193 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 201 0.25755032482093204 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 196 0.25114360032289895 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 191 0.2447368758248658 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 188 0.24089284112604592 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 183 0.23448611662801275 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 166 0.2127032533347001 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 152 0.1947644247402073 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 148 0.18963904514178082 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 142 0.18195097574414107 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 139 0.17810694104532118 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 137 0.1755442512461079 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 136 0.1742629063465013 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 126 0.161449457350435 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 112 0.14351062875594225 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 109 0.13966659405712237 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 108 0.1383852491575157 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 108 0.1383852491575157 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 107 0.1371039042579091 No Hit GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG 107 0.1371039042579091 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 106 0.13582255935830248 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 106 0.13582255935830248 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 105 0.13454121445869585 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 105 0.13454121445869585 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 103 0.1319785246594826 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 100 0.1281344899606627 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 100 0.1281344899606627 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 94 0.12044642056302296 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 92 0.11788373076380969 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 87 0.11147700626577657 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 84 0.10763297156695668 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 79 0.10122624706892355 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 79 0.10122624706892355 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.406724498033135E-4 0.0 22-23 0.0 0.0 0.0 0.0032033622490165677 0.0 24-25 0.0 0.0 0.0 0.0064067244980331355 0.0 26-27 0.0 0.0 0.0 0.015376138795279524 0.0 28-29 0.0 0.0 0.0 0.04164370923721538 0.0 30-31 0.0 0.0 0.0 0.08841279807285726 0.0 32-33 0.0 0.0 0.0 0.1281344899606627 0.0 34-35 0.0 0.0 0.0 0.16593416449905823 0.0 36-37 0.0 0.0 0.0 0.20693720128647028 0.0 38-39 0.0 0.0 0.0 0.2825365503632613 0.0 40-41 0.0 0.0 0.0 0.4356572658662532 0.0 42-43 0.0 0.0 0.0 0.6669400202452495 0.0 44-45 0.0 0.0 0.0 0.8854093256281793 0.0 46-47 0.0 0.0 0.0 1.3268326435426623 0.0 48-49 0.0 0.0 0.0 1.7477544430634393 0.0 50-51 0.0 0.0 0.0 2.070012685314506 0.0 52-53 0.0 0.0 0.0 2.365362684673834 0.0 54-55 0.0 0.0 0.0 2.6754481503786374 0.0 56-57 0.0 0.0 0.0 3.2687108388965056 0.0 58-59 0.0 0.0 0.0 3.931166151993132 0.0 60-61 0.0 0.0 0.0 4.5532591007521495 0.0 62-63 0.0 0.0 0.0 5.17022666991274 0.0 64-65 0.0 0.0 0.0 5.864715605499532 0.0 66-67 0.0 0.0 0.0 6.717450636187742 0.0 68-69 0.0 0.0 0.0 7.689991414989173 0.0 70-71 0.0 0.0 0.0 8.460720372102559 0.0 72-73 0.0 0.0 0.0 9.191086964878336 0.0 74-75 0.0 0.0 0.0 10.053432082313595 0.0 76-77 0.0 0.0 0.0 11.006112015171123 0.0 78-79 0.0 0.0 0.0 11.995950950117244 0.0 80-81 0.0 0.0 0.0 13.179913637353767 0.0 82-83 0.0 0.0 0.0 14.329920684750714 0.0 84-85 0.0 0.0 0.0 15.65739400074318 0.0 86-87 0.0 0.0 0.0 16.847763412477736 0.0 88-89 0.0 0.0 0.0 18.493650936022448 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTAAG 20 0.002056974 71.335686 1 CGCAGAA 40 8.955794E-8 71.24423 9 GGGATTC 25 0.0050053946 56.995388 7 TCTAGAG 40 5.359299E-4 47.496155 2 ATGGGGT 110 3.274181E-11 43.17832 5 GGTGAGA 95 1.8902938E-8 39.99676 9 GGGTGAG 100 2.9713192E-8 37.996925 8 TGGGGTG 145 6.4392225E-10 32.75597 6 CCGCGCC 30 0.0039297827 31.684414 94-95 ATGGGTA 60 0.003930166 31.664104 7 GGGGTGA 135 1.168155E-8 31.664103 7 CTATAGA 65 0.0057770954 29.26592 1 TAGTGTG 100 4.8976646E-5 28.497694 5 CATGGGT 105 6.8287845E-5 27.14066 4 GTACATG 840 0.0 26.609343 1 CATGGGG 270 0.0 26.386753 4 GTGTGAT 90 8.8768825E-4 26.386753 7 TTCTGAC 45 8.9170004E-4 26.386753 24-25 TACATGG 830 0.0 26.323172 2 ATGGGAT 145 7.736071E-7 26.204775 5 >>END_MODULE