FastQCFastQC Report
Thu 26 May 2016
SRR937403_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937403_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821948
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39410.47947072077552355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29730.3617017134903911No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20170.245392652576562No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11260.13699163450729243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40300.036.318741
GGTATCA25750.035.4330941
ATCAACG49600.029.3084243
TATCAAC50200.029.0527552
TCAACGC50150.028.990534
CAACGCA50950.028.443815
AACGCAG52300.027.8845026
GTACATG29750.026.835361
TACATGG31300.025.0433432
ACGCAGA58100.024.9388487
CGCAGAG58200.024.7327468
ACATGGG30350.024.261953
TACCTGG5850.023.5502132
GCAGAGT61500.023.0966389
GCGATGC1050.002193953822.6221268
GAGTACT35250.022.16697912-13
GTAATAG2005.375168E-721.3779093
TAATACG2257.135168E-821.1139833
AGTACTT37650.021.06935512-13
CATGGGG15400.020.9794394
Produced by FastQC (version 0.11.5)