##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937403_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 821948 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.774226593409804 33.0 31.0 34.0 30.0 34.0 2 31.886596232365065 33.0 31.0 34.0 30.0 34.0 3 31.91327431905668 33.0 31.0 34.0 30.0 34.0 4 35.52812221697723 37.0 35.0 37.0 33.0 37.0 5 35.531571340279434 37.0 35.0 37.0 33.0 37.0 6 35.56905424674067 37.0 35.0 37.0 33.0 37.0 7 35.50508061337214 37.0 35.0 37.0 33.0 37.0 8 35.4912902032732 37.0 35.0 37.0 33.0 37.0 9 37.10617581647501 39.0 37.0 39.0 33.0 39.0 10-11 36.98940748076521 39.0 37.0 39.0 33.0 39.0 12-13 36.856469630682234 39.0 37.0 39.0 33.0 39.0 14-15 37.98137108430217 40.0 38.0 41.0 33.0 41.0 16-17 37.906679619635305 40.0 38.0 41.0 33.0 41.0 18-19 37.84498106936205 40.0 38.0 41.0 33.0 41.0 20-21 37.72584275404283 40.0 37.5 41.0 32.0 41.0 22-23 37.783473528739044 40.0 38.0 41.0 33.0 41.0 24-25 37.74153231104644 40.0 38.0 41.0 32.5 41.0 26-27 37.66027473757464 40.0 37.0 41.0 32.0 41.0 28-29 37.52387316472575 40.0 37.0 41.0 32.0 41.0 30-31 37.2756153673955 39.5 37.0 41.0 32.0 41.0 32-33 37.017194518388024 39.0 36.0 41.0 31.0 41.0 34-35 37.033702253670555 39.0 36.0 41.0 31.0 41.0 36-37 36.90061232584056 39.0 36.0 41.0 31.0 41.0 38-39 36.76158589107827 39.0 36.0 41.0 30.0 41.0 40-41 36.53730832120767 39.0 35.5 41.0 30.0 41.0 42-43 36.269828504966256 39.0 35.0 41.0 30.0 41.0 44-45 35.997218802162664 39.0 35.0 41.0 29.5 41.0 46-47 35.57485449201166 39.0 35.0 40.0 27.5 41.0 48-49 35.21018300427764 39.0 35.0 40.0 26.0 41.0 50-51 34.24545652523031 38.0 34.0 39.5 24.5 40.5 52-53 34.34791252001343 38.0 34.0 39.5 24.0 40.5 54-55 34.64596239664796 38.0 34.0 40.0 24.0 41.0 56-57 34.38907814119628 38.0 34.0 40.0 23.0 41.0 58-59 34.11132151912287 38.0 34.0 40.0 22.5 41.0 60-61 33.78100986923747 38.0 33.5 40.0 21.5 41.0 62-63 33.40531456977814 37.0 33.0 40.0 20.0 41.0 64-65 32.97274280124777 37.0 32.0 39.5 18.0 41.0 66-67 32.53407818986116 36.0 32.0 39.0 15.5 40.5 68-69 32.053503992953324 35.5 31.0 39.0 10.5 40.0 70-71 31.582265544778988 35.0 31.0 38.5 7.5 40.0 72-73 31.08704565252303 35.0 30.5 38.0 6.5 40.0 74-75 30.53368278771893 35.0 30.0 37.0 2.0 39.0 76-77 30.012845703134506 34.0 29.0 36.5 2.0 39.0 78-79 29.461450724376725 34.0 29.0 36.0 2.0 38.5 80-81 28.88185992301216 34.0 28.5 35.5 2.0 37.5 82-83 28.32410809442933 34.0 27.5 35.0 2.0 37.0 84-85 27.757111155450225 33.5 26.0 35.0 2.0 36.5 86-87 27.165130884192187 33.0 25.5 35.0 2.0 36.0 88-89 26.607442319952114 32.5 25.0 35.0 2.0 35.5 90-91 26.046236501579173 32.0 23.5 35.0 2.0 35.0 92-93 25.38040605975074 32.0 20.5 34.0 2.0 35.0 94-95 24.614908120708368 31.0 16.5 34.0 2.0 35.0 96-97 23.751145449590485 31.0 4.5 34.0 2.0 35.0 98-99 22.555217605006643 31.0 2.0 34.0 2.0 35.0 100-101 19.48861302661482 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1666885486790015 1101 2 0.2071550127350612 1101 3 0.04800820318666155 1101 4 0.11877099312896888 1101 5 0.1362479864629833 1101 6 0.11476427052332383 1101 7 0.18727730350441618 1101 8 0.18914769105202112 1101 9 0.2087770575456247 1101 10-11 0.34871381043831207 1101 12-13 0.3974160813193208 1101 14-15 0.4030029193242868 1101 16-17 0.4251549360937261 1101 18-19 0.37942951717706563 1101 20-21 0.4157715954727266 1101 22-23 0.4685401522914887 1101 24-25 0.4642204929030669 1101 26-27 0.3025067122238667 1101 28-29 0.39555140065712635 1101 30-31 0.3578020057567599 1101 32-33 0.4504413543205317 1101 34-35 0.4466635484583392 1101 36-37 0.49483266932127634 1101 38-39 0.6400962132670003 1101 40-41 0.8014283249024388 1101 42-43 0.7985982556045599 1101 44-45 0.9000751103357274 1101 46-47 1.1458044990766965 1101 48-49 1.0963028610328323 1101 50-51 1.1404008149040408 1101 52-53 1.069057991098994 1101 54-55 1.2243954005336235 1101 56-57 1.348340370161175 1101 58-59 1.104915366332051 1101 60-61 1.064205876550929 1101 62-63 1.3168725104850978 1101 64-65 1.2982657673682922 1101 66-67 1.426457116149031 1101 68-69 1.3547186231255424 1101 70-71 1.331124401703903 1101 72-73 1.222887202798816 1101 74-75 1.4494552500931057 1101 76-77 1.539716229638703 1101 78-79 1.6632032693401868 1101 80-81 1.6506262878089117 1101 82-83 1.4880891336724211 1101 84-85 1.7529635313572776 1101 86-87 1.8747195714700204 1101 88-89 1.868217778605949 1101 90-91 1.7666632974229088 1101 92-93 1.836756938461452 1101 94-95 1.7513488071073446 1101 96-97 1.74692964397234 1101 98-99 1.8299554150812405 1101 100-101 2.15434553612619 1102 1 0.10386321907126117 1102 2 0.1639695027050756 1102 3 0.2188480707816609 1102 4 0.19437069787399253 1102 5 0.14771465789731764 1102 6 0.11643657325672763 1102 7 0.030486311335309324 1102 8 -0.047117588903844876 1102 9 0.08652208597285238 1102 10-11 0.12425457912289772 1102 12-13 0.21434362891513814 1102 14-15 0.3438009123055963 1102 16-17 0.4176739514337058 1102 18-19 0.32406601641338995 1102 20-21 0.2420832580443033 1102 22-23 0.27989112259742654 1102 24-25 0.49006168863463984 1102 26-27 0.42673122094104343 1102 28-29 0.5163546806436372 1102 30-31 0.6117273517107904 1102 32-33 0.6329487101538618 1102 34-35 0.5744791341410291 1102 36-37 0.4948882211875656 1102 38-39 0.749818172983602 1102 40-41 0.9290264920739659 1102 42-43 0.9573425024744964 1102 44-45 1.0732823175199329 1102 46-47 1.2871316395475745 1102 48-49 1.5384344180686327 1102 50-51 1.5687864776697324 1102 52-53 1.6240278398446648 1102 54-55 1.887157260537819 1102 56-57 1.7395461904642957 1102 58-59 1.7236501374583568 1102 60-61 1.5239227919026561 1102 62-63 1.7098192751954926 1102 64-65 1.6745709169624448 1102 66-67 1.6998415784835856 1102 68-69 1.6462002233257849 1102 70-71 1.686927081474856 1102 72-73 1.8941347700101936 1102 74-75 1.3635241752730423 1102 76-77 1.5360644925893112 1102 78-79 1.519813366753894 1102 80-81 1.603783293984744 1102 82-83 1.654479743746851 1102 84-85 1.5974045774180716 1102 86-87 1.6502079809243106 1102 88-89 1.6932115210394052 1102 90-91 1.9884783876084917 1102 92-93 1.9891842357546032 1102 94-95 1.6930490390204227 1102 96-97 1.4984426341696668 1102 98-99 1.4327483969010935 1102 100-101 1.3481273667070433 1103 1 0.44760521485266125 1103 2 0.48324859911862816 1103 3 0.3256828604980413 1103 4 0.3519242312923865 1103 5 0.33266018304230727 1103 6 0.29730401175422116 1103 7 0.26756213291440645 1103 8 0.32695673199717845 1103 9 0.5013861055900648 1103 10-11 0.38685437341196405 1103 12-13 0.39516168715493905 1103 14-15 0.45341785800417256 1103 16-17 0.36807369064170814 1103 18-19 0.383087869786948 1103 20-21 0.2925856118365928 1103 22-23 0.38334276246359167 1103 24-25 0.49117856568261686 1103 26-27 0.4645705496264796 1103 28-29 0.3961611260921032 1103 30-31 0.5162873575561306 1103 32-33 0.4209227305178018 1103 34-35 0.6624956295558988 1103 36-37 0.5878503702163798 1103 38-39 0.47714681345277654 1103 40-41 0.6402981180173057 1103 42-43 0.4126646102968934 1103 44-45 0.7984595043226292 1103 46-47 0.6212653612854027 1103 48-49 0.5866596594899178 1103 50-51 0.6833237846551512 1103 52-53 0.7571614307047412 1103 54-55 1.0231320228100174 1103 56-57 0.8460634209625155 1103 58-59 0.8461248190359925 1103 60-61 0.8868556419785776 1103 62-63 1.0294026824653884 1103 64-65 0.5525808042550153 1103 66-67 0.8212327046505408 1103 68-69 1.060432737696246 1103 70-71 1.3059367142382996 1103 72-73 1.263079164628298 1103 74-75 1.1052513762474803 1103 76-77 1.0779614331812475 1103 78-79 0.9953749567821326 1103 80-81 1.0237760354883285 1103 82-83 1.1627057720405318 1103 84-85 1.3560603745398865 1103 86-87 1.3395644076243372 1103 88-89 1.4502811279324028 1103 90-91 1.211755839619311 1103 92-93 1.274248434104262 1103 94-95 1.3495427034932135 1103 96-97 1.5397498219278596 1103 98-99 1.8634895744957873 1103 100-101 1.580649365009645 1104 1 0.18587743275316626 1104 2 0.2167879408715372 1104 3 0.17165691347470613 1104 4 0.3511158513860906 1104 5 0.12604204306890665 1104 6 0.11712760210814821 1104 7 0.14126218274358848 1104 8 0.10556728646334079 1104 9 0.27954586727035036 1104 10-11 0.2927421932239085 1104 12-13 0.30038407228791897 1104 14-15 0.3754226401717773 1104 16-17 0.3651565325541455 1104 18-19 0.3562352279440546 1104 20-21 0.2276943039818562 1104 22-23 0.38597178957568445 1104 24-25 0.2707408933865665 1104 26-27 0.10857240657011857 1104 28-29 0.15214107611952699 1104 30-31 0.15800926052903463 1104 32-33 -0.012513636898937364 1104 34-35 0.29400344628487574 1104 36-37 0.3763582012846953 1104 38-39 0.3398647265062209 1104 40-41 0.2782568422468401 1104 42-43 0.1831456590757199 1104 44-45 0.15535340759665672 1104 46-47 0.3295741982579372 1104 48-49 0.2612656863116385 1104 50-51 0.32376142110286565 1104 52-53 0.5285292776180199 1104 54-55 0.6471067604898337 1104 56-57 0.737317951295573 1104 58-59 0.6743328863448212 1104 60-61 0.7300111197830788 1104 62-63 0.6688307402385547 1104 64-65 0.8720051338015722 1104 66-67 0.7629724421690511 1104 68-69 0.7874777058449993 1104 70-71 1.0351092809430753 1104 72-73 1.1236287016470818 1104 74-75 1.151264952011136 1104 76-77 1.0406370452019118 1104 78-79 0.9794349955700277 1104 80-81 0.8717759673764967 1104 82-83 0.8530998527638083 1104 84-85 0.8955773361785866 1104 86-87 0.7895555351619521 1104 88-89 0.9001355837009406 1104 90-91 0.9055738842361443 1104 92-93 0.9974235747193454 1104 94-95 0.9106712807087334 1104 96-97 0.9868762195647385 1104 98-99 0.8813828217453228 1104 100-101 0.6268650357498764 1105 1 -0.039188303034300986 1105 2 -0.028057466690341926 1105 3 -0.10156459346154989 1105 4 -0.04601353477765713 1105 5 -0.11684942487922712 1105 6 -0.12697521773045395 1105 7 -0.11370653608858561 1105 8 -0.07342899606454267 1105 9 -0.05209979416768107 1105 10-11 -0.096818510375698 1105 12-13 0.01965871187446311 1105 14-15 0.12896693249202684 1105 16-17 0.1395975516855188 1105 18-19 0.07183637038670554 1105 20-21 -0.019812722947143868 1105 22-23 0.0526999607607479 1105 24-25 -0.13090295465042345 1105 26-27 0.019783758460583556 1105 28-29 -0.1113238799054983 1105 30-31 0.03606438792045452 1105 32-33 0.2275276179745731 1105 34-35 0.19232605032876648 1105 36-37 0.33743853495048626 1105 38-39 0.10396573294379152 1105 40-41 0.12129066217611495 1105 42-43 0.16631185728044784 1105 44-45 0.14554608145648018 1105 46-47 0.3173958502662799 1105 48-49 0.1284979829840509 1105 50-51 0.16137679874363187 1105 52-53 0.3815916586135799 1105 54-55 0.5522436722678776 1105 56-57 0.678824434204266 1105 58-59 0.6410510838241947 1105 60-61 0.6626949533128581 1105 62-63 0.7044046816657072 1105 64-65 0.7261791296307507 1105 66-67 0.8188501282693323 1105 68-69 0.7524251172150258 1105 70-71 0.7034332450377399 1105 72-73 0.611724177211503 1105 74-75 0.5565052276480955 1105 76-77 0.5726063987244423 1105 78-79 0.6215367273680172 1105 80-81 0.6999267516724466 1105 82-83 0.8714895526459365 1105 84-85 0.8924895675685569 1105 86-87 0.7547042340467094 1105 88-89 0.8403994335513332 1105 90-91 0.9410471819059261 1105 92-93 1.323138054726602 1105 94-95 0.8310738557382003 1105 96-97 0.682981940845 1105 98-99 0.9703179019883201 1105 100-101 1.1762657067082607 1106 1 0.008370876533930982 1106 2 -0.7484353538270945 1106 3 -0.12969922473422457 1106 4 0.0613911852194704 1106 5 0.02466427891760503 1106 6 -0.1463954555263527 1106 7 0.05173762498041867 1106 8 0.08327203486329182 1106 9 0.02008383311778772 1106 10-11 -0.2385463023222414 1106 12-13 -0.3267107697879581 1106 14-15 -0.3015241052607678 1106 16-17 -0.10484226296299681 1106 18-19 0.01002236844697535 1106 20-21 -0.08328195675583316 1106 22-23 -0.15573137657177227 1106 24-25 -0.09188646698923719 1106 26-27 -0.0838478375019136 1106 28-29 0.1732078920690654 1106 30-31 0.2153768632248827 1106 32-33 0.06361583953426475 1106 34-35 0.3040362410683741 1106 36-37 0.004808770950340602 1106 38-39 0.3829154078602812 1106 40-41 0.3605011223219563 1106 42-43 0.0940413015852073 1106 44-45 -0.13484689242907422 1106 46-47 0.36673454777696435 1106 48-49 0.29291552671766397 1106 50-51 0.04323462409655576 1106 52-53 0.13880994950046244 1106 54-55 0.45974466009072046 1106 56-57 0.2842879925217403 1106 58-59 -0.07996834934510133 1106 60-61 -0.02278656541984958 1106 62-63 0.32333783300016705 1106 64-65 0.6738259560253752 1106 66-67 0.786219645852583 1106 68-69 0.6388082487930262 1106 70-71 0.43251403098550867 1106 72-73 0.3519705941086606 1106 74-75 0.3172789623368395 1106 76-77 0.659572884589597 1106 78-79 0.8787895022521717 1106 80-81 0.5837300492046857 1106 82-83 0.39837198376774197 1106 84-85 0.431746724794035 1106 86-87 0.49697227528382015 1106 88-89 0.7601623560701007 1106 90-91 0.8231277164078854 1106 92-93 0.9119566186118 1106 94-95 0.8673208473686458 1106 96-97 0.7035969054629838 1106 98-99 0.645725064601919 1106 100-101 0.7684766338248927 1107 1 -0.07969088987835349 1107 2 -0.05844918878938188 1107 3 -0.014203174392815043 1107 4 -0.016952189618749003 1107 5 -0.06354565547789548 1107 6 0.03751849993399503 1107 7 0.07668518601045804 1107 8 -0.26736839000393786 1107 9 -4.8116889051641465E-4 1107 10-11 0.01027683330501361 1107 12-13 0.025586280241050474 1107 14-15 -0.012156496924085047 1107 16-17 0.06889540830045604 1107 18-19 0.09066859507259295 1107 20-21 0.11600398067443507 1107 22-23 0.06953662003643046 1107 24-25 0.00730842746339988 1107 26-27 0.004957446561107304 1107 28-29 0.07853569141749261 1107 30-31 0.12230237757308515 1107 32-33 0.03781586631160394 1107 34-35 0.2509956733886298 1107 36-37 0.1817400870569159 1107 38-39 0.35166713723056375 1107 40-41 0.14733648627072427 1107 42-43 0.13484105166330096 1107 44-45 0.24026891959395158 1107 46-47 0.31511942750204724 1107 48-49 0.3908483155782889 1107 50-51 0.39311367974880795 1107 52-53 0.2542006756128359 1107 54-55 0.36333014676898756 1107 56-57 0.5451511156529065 1107 58-59 0.4676881865588598 1107 60-61 0.7266417382352515 1107 62-63 0.7158607053168495 1107 64-65 0.6219662693203318 1107 66-67 0.6303504978184833 1107 68-69 0.7291139568306875 1107 70-71 0.8315928902705565 1107 72-73 0.9067722186010094 1107 74-75 0.7639849024448431 1107 76-77 1.0287039597000494 1107 78-79 1.0946631131773294 1107 80-81 0.8893872099133908 1107 82-83 0.9831820877531072 1107 84-85 0.8306122578863686 1107 86-87 0.8801587795012544 1107 88-89 0.7044940381337419 1107 90-91 0.5229688374861183 1107 92-93 0.5940175817036888 1107 94-95 0.7225446613553466 1107 96-97 0.9244305735131455 1107 98-99 0.8390244799631894 1107 100-101 1.095541390374187 1108 1 0.041277724840263375 1108 2 -0.05028089874609165 1108 3 0.06155169573705521 1108 4 -0.05785586927243003 1108 5 -0.08869175937400087 1108 6 0.10627391118940466 1108 7 0.03440975311002603 1108 8 -0.10066113892168715 1108 9 0.014169805135459512 1108 10-11 0.027216768369946465 1108 12-13 0.09846642093369695 1108 14-15 0.2298064359763501 1108 16-17 0.20070817886322345 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0.23890918060189748 2315 86-87 0.2327404097573016 2315 88-89 0.48975595967473495 2315 90-91 0.5537792673663127 2315 92-93 0.7998103935430123 2315 94-95 1.182258074557133 2315 96-97 1.3990089150682294 2315 98-99 1.5916061102192387 2315 100-101 2.5923607467284278 2316 1 -0.10964297194624706 2316 2 0.052631326834561065 2316 3 -0.44826675001541005 2316 4 -0.10781808689890227 2316 5 -0.0050236874681814925 2316 6 -0.08448730420989392 2316 7 0.07108351225733145 2316 8 -0.027994697623562104 2316 9 -0.2510177147499988 2316 10-11 -0.2801179834595118 2316 12-13 -0.12073348759319202 2316 14-15 -0.11573076971884433 2316 16-17 -0.2638062182096377 2316 18-19 0.10668128320109105 2316 20-21 0.19635762851137883 2316 22-23 0.07702936485656409 2316 24-25 0.12359647497638804 2316 26-27 -0.08869578958655921 2316 28-29 0.02882387391057506 2316 30-31 0.20960884986840966 2316 32-33 0.06731576507654324 2316 34-35 0.02047532513999073 2316 36-37 -0.16478755011659985 2316 38-39 -0.22334264882599086 2316 40-41 -0.12061384105600581 2316 42-43 -0.24561383124147085 2316 44-45 -0.22509653900090143 2316 46-47 -0.33050789246825474 2316 48-49 -0.2589309919738483 2316 50-51 -0.19482179976795777 2316 52-53 -0.024557507555144298 2316 54-55 -0.3028532911739177 2316 56-57 -0.007117224766879815 2316 58-59 -0.0955864304351337 2316 60-61 -0.12755546767687065 2316 62-63 0.1796819916771213 2316 64-65 0.08927544121747388 2316 66-67 -0.02768689166656202 2316 68-69 0.27383421059326096 2316 70-71 0.39560989776195044 2316 72-73 0.5352300710677689 2316 74-75 0.4554177161048294 2316 76-77 0.4666995053570666 2316 78-79 0.3215997400248689 2316 80-81 0.8682520273528205 2316 82-83 0.7154126337475724 2316 84-85 0.944381576911816 2316 86-87 0.8805394233562858 2316 88-89 1.0509312386466654 2316 90-91 1.322351978472824 2316 92-93 1.0125139034962238 2316 94-95 1.0486565598503184 2316 96-97 0.9992891818684129 2316 98-99 1.736435561828614 2316 100-101 2.0735909280673788 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3305.0 3 647.0 4 603.0 5 734.0 6 787.0 7 917.0 8 1120.0 9 1413.0 10 1862.0 11 2365.0 12 3038.0 13 4184.0 14 4595.0 15 5885.0 16 7472.0 17 10104.0 18 8722.0 19 6189.0 20 6192.0 21 6535.0 22 7073.0 23 7879.0 24 8898.0 25 9895.0 26 11624.0 27 13594.0 28 15715.0 29 18828.0 30 22926.0 31 28079.0 32 35573.0 33 45562.0 34 60606.0 35 83213.0 36 115533.0 37 146732.0 38 103228.0 39 10321.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1730379938246 18.851185569273245 15.228016604311073 22.747759832591083 2 14.904996738739506 19.858820947655246 41.50127707303395 23.7349052405713 3 18.73056164131374 24.42366142783469 30.756344936303755 26.089431994547812 4 12.321116375242921 16.467046887077462 38.36930309451297 32.84253364316664 5 13.281022356115349 36.66417547423079 35.317224705964115 14.737577463689751 6 31.094100438810337 36.23671379814725 18.21428571428571 14.454900048756706 7 27.716607609317045 32.786275778929266 21.59268347089003 17.904433140863663 8 25.999924425911185 34.37389914759741 20.63891797407809 18.98725845241331 9 26.022681420276744 16.50333589737651 21.121417399816416 36.35256528253033 10-11 24.202480595772123 26.449792836665566 28.363912555554947 20.983814012007365 12-13 25.460800490561507 23.84904261874996 28.942275515385212 21.747881375303322 14-15 22.282048218913573 25.118791918492533 27.482440250529656 25.116719612064237 16-17 21.371768899030457 28.551255140872712 28.032292116150202 22.04468384394663 18-19 21.20887465278159 26.881439309451288 30.837322002952046 21.072364034815084 20-21 21.94940752798724 25.838642107656028 30.967548879017233 21.244401485339495 22-23 21.64311998508094 25.828684331451797 31.039141812738976 21.48905387072829 24-25 21.6069768930033 25.986854334199755 30.98050090045068 21.42566787234626 26-27 21.37714125177263 26.168718664701103 30.917758990225614 21.53638109330065 28-29 21.136186343580118 26.30969503425075 31.069208454206382 21.484910167962752 30-31 21.285983336279248 26.06183983567889 31.03017632825214 21.622000499789724 32-33 21.068647670869538 26.269576007567114 30.92850734770695 21.7332689738564 34-35 21.60350904351504 26.210134270768513 30.70029114042086 21.486065545295585 36-37 21.489864300715368 26.01926825017034 30.76057663358999 21.7302908155243 38-39 21.310265560934706 26.30123395396684 30.810821352132727 21.57767913296573 40-41 21.51141993007696 26.20041963573473 30.740480953985717 21.547679480202593 42-43 21.35879846321866 26.259482993993295 30.519212204540136 21.862506338247915 44-45 21.395302068637697 26.467715052698615 30.269145334824355 21.867837543839336 46-47 21.611504845996468 26.576830511707726 29.862222923760346 21.949441718535457 48-49 21.682509441977167 26.835814728204525 29.5085174343663 21.973158395452007 50-51 21.596266451147716 26.955593710211122 29.48215111803502 21.965988720606138 52-53 21.812637482865117 27.066501936112257 29.213992670018573 21.906867911004056 54-55 21.587666489110784 26.84023261851855 29.338772962911946 22.233327929458717 56-57 21.64121346561685 27.098822611705632 29.223240621114982 22.03672330156254 58-59 21.890342626733865 26.797058237465244 29.308975861289397 22.00362327451149 60-61 21.771663619744057 26.56782449725777 29.75862279098111 21.90188909201706 62-63 21.61365810851164 26.744224311823174 29.695111918241484 21.947005661423706 64-65 21.490666273419627 26.765803107545345 29.681884270809082 22.061646348225942 66-67 21.295975799708952 26.918923662317212 29.370641302837587 22.41445923513625 68-69 21.33213162266285 26.751712681460138 29.605348953134502 22.31080674274251 70-71 21.421628522762937 26.706054779744477 29.532857791442073 22.339458906050513 72-73 21.50021603510677 26.524296788968744 29.431660082014115 22.54382709391037 74-75 21.32972885349142 26.71298618275956 29.381914181121456 22.57537078262756 76-77 21.470874307082507 26.658796944093687 29.346061400900002 22.524267347923807 78-79 21.375114423239832 26.567644094054376 29.238352597879864 22.818888884825924 80-81 21.27661649154434 26.777170166980223 29.23899947161629 22.707213869859153 82-83 21.361353858154473 26.619864224164196 29.471522420076298 22.547259497605033 84-85 21.313397401796525 26.40706568997908 29.19868109395347 23.080855814270922 86-87 21.305835223401832 26.335164292756886 29.36057388207679 22.998426601764496 88-89 21.481524365533275 25.95821310281576 29.278616894533332 23.281645637117638 90-91 21.281649860478268 25.787296849471925 29.877334223867326 23.05371906618248 92-93 21.361408358517853 25.61465156997213 29.822309735759088 23.201630335750924 94-95 20.98735564169707 25.795063770247946 29.801903631330024 23.41567695672496 96-97 20.830050004113684 25.816505318840978 29.92933605141191 23.42410862563343 98-99 21.044109303364433 26.194572372129706 29.412560538387144 23.348757786118725 100-101 21.138770734561056 25.811344440517253 29.76063084254531 23.289253982376383 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1046.0 1 598.0 2 156.0 3 151.0 4 125.5 5 122.0 6 198.5 7 427.5 8 752.5 9 964.5 10 960.5 11 924.5 12 1001.5 13 1082.0 14 1132.5 15 1251.0 16 1339.5 17 1369.0 18 1485.0 19 1625.0 20 1814.0 21 2040.0 22 2311.0 23 2765.0 24 3331.5 25 4129.5 26 5182.0 27 6347.0 28 7495.0 29 8896.0 30 10543.5 31 12176.5 32 13875.0 33 15625.0 34 17504.5 35 19048.5 36 20947.0 37 22737.5 38 23925.0 39 24780.5 40 25502.5 41 27205.0 42 28873.5 43 30124.5 44 31314.5 45 32096.0 46 32094.5 47 32425.5 48 34018.0 49 33553.5 50 31372.0 51 29457.5 52 27570.0 53 26376.0 54 24824.0 55 22334.5 56 19792.0 57 17208.5 58 14897.5 59 12893.0 60 10829.0 61 8789.5 62 6825.0 63 5202.0 64 3903.5 65 2918.5 66 2139.0 67 1643.5 68 1228.5 69 823.0 70 558.0 71 392.5 72 283.5 73 220.0 74 176.5 75 144.5 76 96.0 77 58.5 78 34.5 79 20.0 80 20.5 81 13.5 82 7.0 83 5.0 84 4.0 85 4.0 86 2.5 87 1.5 88 1.5 89 2.5 90 2.5 91 0.5 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23359141940852707 2 0.20840734450354523 3 0.21011061527006575 4 0.2095023042820227 5 0.2098672908748485 6 0.18833308189812495 7 0.1896713660718196 8 0.18991469046703682 9 0.19818771990442216 10-11 0.20700822923104623 12-13 0.20238506572191917 14-15 0.19538948935942418 16-17 0.19003635266464544 18-19 0.18237163421530317 20-21 0.1860823312423657 22-23 0.18504820256269253 24-25 0.18559568245193125 26-27 0.18097251894280417 28-29 0.18589983794595277 30-31 0.19502450276659838 32-33 0.1893063794789938 34-35 0.1890022239849723 36-37 0.1862648245387786 38-39 0.18200664762247734 40-41 0.17939091037389227 42-43 0.18638648673638722 44-45 0.18012088355954392 46-47 0.18596066904475708 48-49 0.1876639398112776 50-51 0.19496367166779408 52-53 0.19691026682953178 54-55 0.18845474409573354 56-57 0.18103335004160848 58-59 0.17269948950541883 60-61 0.17616686213726415 62-63 0.1804250390535654 64-65 0.1738552803827006 66-67 0.17787013290378467 68-69 0.17653184873008998 70-71 0.18012088355954392 72-73 0.1799992213619353 74-75 0.18097251894280417 76-77 0.18297994520334618 78-79 0.1860215001435614 80-81 0.18510903366149684 82-83 0.1798167280655224 84-85 0.17269948950541883 86-87 0.17409860477791783 88-89 0.17951257257150088 90-91 0.1784176127930234 92-93 0.1829191141045419 94-95 0.17805262620019757 96-97 0.1843790604758452 98-99 0.18218914091889027 100-101 0.17738348411335025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 821948.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.74947365876905 #Duplication Level Percentage of deduplicated Percentage of total 1 84.21467845636602 57.055001397612756 2 9.639420584457914 13.061313419450551 3 2.645665932185763 5.377274231475661 4 1.1128985183412199 3.015931554129664 5 0.612590093383366 2.0751328197649617 6 0.36821122963760144 1.4967670201917387 7 0.26517278389443183 1.257572157623479 8 0.19295617236972062 1.0458143293807431 9 0.13612756645808644 0.8300313880186014 >10 0.744188559577503 9.16560325627034 >50 0.04845699253393026 2.188913784993256 >100 0.018732509392782266 2.1041726508414715 >500 1.8012028033701554E-4 0.0992108305492752 >1k 7.204811213480622E-4 1.2272611596974916 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3941 0.47947072077552355 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2973 0.3617017134903911 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2017 0.245392652576562 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1126 0.13699163450729243 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 6.083109880430392E-4 0.0 26-27 0.0 0.0 0.0 0.0012166219760860785 0.0 28-29 0.0 0.0 0.0 0.0031023860390195 0.0 30-31 0.0 0.0 0.0 0.006691420868473432 0.0 32-33 0.0 0.0 0.0 0.012227050859665089 0.0 34-35 0.0 0.0 0.0 0.01721520096161801 0.0 36-37 0.0 0.0 0.0 0.025001581608568912 0.0 38-39 0.0 0.0 0.0 0.034552064120844626 0.0 40-41 0.0 0.0 0.0 0.04452836432475047 0.0 42-43 0.0 0.0 0.0 0.06940828373571077 0.0 44-45 0.0 0.0 0.0 0.09878970445818958 0.0 46-47 0.0 0.0 0.0 0.13066520023164482 0.0 48-49 0.0 0.0 0.0 0.167528846107053 0.0 50-51 0.0 0.0 0.0 0.21491627207560576 0.0 52-53 0.0 0.0 0.0 0.3019655744645646 0.0 54-55 0.0 0.0 0.0 0.3755103729189681 0.0 56-57 0.0 0.0 0.0 0.5040464846924624 0.0 58-59 0.0 0.0 0.0 0.6807608267189652 0.0 60-61 0.0 0.0 0.0 0.8381917104245038 0.0 62-63 0.0 0.0 0.0 1.0703840145605317 0.0 64-65 0.0 0.0 0.0 1.2302481422182425 0.0 66-67 0.0 0.0 0.0 1.515181009017602 0.0 68-69 0.0 0.0 0.0 1.855470175728878 0.0 70-71 0.0 0.0 0.0 2.116435589599342 0.0 72-73 0.0 0.0 0.0 2.579603575895312 0.0 74-75 0.0 0.0 0.0 3.0233664416727093 0.0 76-77 0.0 0.0 0.0 3.336403276119657 0.0 78-79 0.0 0.0 0.0 3.680281477660387 0.0 80-81 0.0 0.0 0.0 4.265111661564966 0.0 82-83 0.0 0.0 0.0 4.792273963803063 0.0 84-85 0.0 0.0 0.0 5.470723208767465 0.0 86-87 0.0 0.0 0.0 6.3386004954084685 0.0 88-89 0.0 0.0 0.0 7.54013635899108 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4030 0.0 36.31874 1 GGTATCA 2575 0.0 35.433094 1 ATCAACG 4960 0.0 29.308424 3 TATCAAC 5020 0.0 29.052755 2 TCAACGC 5015 0.0 28.99053 4 CAACGCA 5095 0.0 28.44381 5 AACGCAG 5230 0.0 27.884502 6 GTACATG 2975 0.0 26.83536 1 TACATGG 3130 0.0 25.043343 2 ACGCAGA 5810 0.0 24.938848 7 CGCAGAG 5820 0.0 24.732746 8 ACATGGG 3035 0.0 24.26195 3 TACCTGG 585 0.0 23.550213 2 GCAGAGT 6150 0.0 23.096638 9 GCGATGC 105 0.0021939538 22.622126 8 GAGTACT 3525 0.0 22.166979 12-13 GTAATAG 200 5.375168E-7 21.377909 3 TAATACG 225 7.135168E-8 21.113983 3 AGTACTT 3765 0.0 21.069355 12-13 CATGGGG 1540 0.0 20.979439 4 >>END_MODULE