FastQCFastQC Report
Thu 26 May 2016
SRR937403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937403_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821948
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33030.4018502387012317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28030.3410191398969278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17240.20974562867723995No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9720.11825565607556682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31300.033.4064981
GTATCAA44550.028.4849951
GTGTAGC3200.026.734691
GTACATG33550.023.6579191
ATCAACG53600.023.4838543
TCAACGC53650.023.461974
TATCAAC54550.023.2490272
CAACGCA54650.023.0326565
AACGCAG56050.022.372616
TACATGG35050.022.2251222
ACATGGG34150.022.1153963
GAGTACT32350.021.3637312-13
GTGTTAC2951.3278623E-1020.9446891
GTACTTT34200.020.13864314-15
ACGCAGA62800.019.967917
CGCAGAG63400.019.7789388
CTACACT3850.019.740024
AGTACTT33450.019.66717512-13
CATGGGG16350.019.4645944
CGGTTAC1101.3456247E-619.43158128-29
Produced by FastQC (version 0.11.5)