FastQCFastQC Report
Thu 26 May 2016
SRR937402_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937402_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798247
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41630.5215177758262793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30720.38484328785451116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19110.23939958433918324No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8880.11124376289544465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.043.1105421
GTATCAA36200.042.1512451
ATAGCGT704.3071395E-640.7161946
TATCAAC46350.032.6007582
ATCAACG46500.032.4955943
TCAACGC47150.031.9528564
CAACGCA47700.031.4847935
AACGCAG49100.030.6684466
ACGCAGA54100.027.7462187
CGCAGAG53900.027.5847828
GCAGAGT57100.025.2901549
GTACATG29450.025.0184461
CGTACAC950.001223647725.0090223
TACCTGG5500.024.1905442
GAGTACT32300.023.82464612-13
TACATGG31200.023.4539742
AGAGTAC50200.022.99410610-11
AGTACTT34300.022.98941812-13
ACATGGG31250.022.6561743
GTACTTT35850.021.06793414-15
Produced by FastQC (version 0.11.5)