FastQCFastQC Report
Thu 26 May 2016
SRR937402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937402_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798247
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33660.42167399313746246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27940.3500169746958022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18140.2272479570859646No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9080.11374925305074744No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32050.033.5069121
GTATCAA46100.028.7579081
CGTAGCA908.9422136E-426.3903687
TATCAAC54750.024.2038042
ATCAACG55700.023.5351753
TCAACGC56050.023.388214
CAACGCA56700.023.2038615
TACGTAG1252.2561214E-422.7984225
AACGCAG58000.022.6837736
GTGTAGC3800.022.508311
GTACATG33800.021.9311751
ACGTAGC1100.002877760421.5894156
GAGTACT33150.021.56606512-13
GTACTTT33900.020.73602714-15
GGGCGTA1150.00372108720.6533329
TTACGTA1404.8522168E-420.3557344
CGCAGAG64750.020.1748778
TACATGG35950.020.082062
ACGCAGA65350.019.9896437
ACATGGG34550.019.9334983
Produced by FastQC (version 0.11.5)